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- ArticleYalnız künye2017Oxidative renal damage in rats subjected to Ovarian Ischemia reperfusion and protective effect of tianeptine
Karaca, Ibrahim; Delibaş, Ilhan B.; Yapça, Ömer E.; Ingeç, Metin; Kumbasar, Serkan; Hirik, Erkan; Kuzucu, Mehmet; Bilgin, Aslı Ö.
Ischemia reperfusion (I/R) injury in an organ can also cause damage in other organs. The aim of this study was to research the effect of tianeptine on oxidative ovarian injury induced by I/R in rats, and on the complication of this damage on the kidney. Albino Wistar female rats were assigned into groups, control subjected to ovarian I/R (OIR), exposed to 25 mg/kg tianeptine + ovarian I/R (TOIR), sham operation + 25 mg/kg tianeptin (TSG) and sham operated (SHAM). MDA, MPO, TNF-α and IL-1β levels in OIR group were found to be significantly higher compared to the TOIR,TSG and SHAM groups, and the tGSH level was found to be lower. Histopathologically, dilated and congested blood vessel, edema, hemorrhage, and PNL infiltration were observed in the OIR group ovarian and kidney tissues. In the ovarian and kidney tissues of TOIR group, dilated and congested blood vessel was found. This information obtained suggests that tianeptine may be useful in the treatment of ovarian I/R injury and its complications on kidney.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2016Effects of nimesulide on the small intestine mucositis induced by methotrexate in rats
Arslan, Aynur; Ozcicek, Adalet; Suleyman, Bahadir; Coban, Taha Abdulkadir; Cimen, Ferda Keskin; Nalkiran, Hatice Sevim; Kuzucu, Mehmet; Altuner, Durdu; Cetin, Nihal; Suleyman, Halis
Intestinal mucositis is one of the major problems in the patients receiving cancer treatment. Nimesulide is a drug with antioxidant, antiinflammatory and antiulcer features. We aimed to investigate the effect of nimesulide on the small intestine mucositis induced by methotrexate (MTX) in rats. Experimental animals were divided into the control group, MTX group (MTXG) and nimesulide+MTX administered group (NMTXG) with eight rats per group. The control and MTXG groups were given distilled water by gavage and the NMTXG was given nimesulide 100 mg/kg orally. After one hour, the NMTXG and MTXG rat groups were administered oral MTX 5 mg/kg. This procedure was repeated once a day for 15 days and the rats were sacrificed. The duodenum and jejunum of each rat was removed for the assessment of biochemical markers and histopathological evaluation. Malondialdehyde (MDA) and myeloperoxidase (MPO) levels were significantly higher in the duodenal and jejunal tissues of the animals which received MTX, compared to the control and NMTXG (P<0.001). Also, the levels of total glutathione (tGSH), glutathione reductase (GSHRd), glutathione peroxidase (GSHPx), catalase (CAT) and superoxide dismutase (SOD) were significantly lower in the MTXG (P<0.001) compared to other groups. MTX led to villus and crypt epithelial damage and inflammation containing marked PMNL and eosinophils in the intestinal tissues histopathologically. Whereas, there was only mild irregularities in the villus structures of the NMTXG. Nimesulide protected the small intestines against damage by MTX. Intestinal mucositis caused by MTX may be preventable by co-administered nimesulide.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2016Can thıamıne pyrophosphate prevent desflurane ınduced hepatotoxıcıty ın rats?
Arslan, Aynur; Kuyrukluyildiz, Ufuk; Binici, Orhan; Cetin, Nihal; Balci, Mecdi Gurhan; Kuzucu, Mehmet; Yilmaz, Adnan; Altuner, Durdu; Coban, Taha Abdulkadir
PURPOSE: To investigate the effects of thiamine pyrophosphate (TPP) against desflurane induced hepatotoxicity. METHODS: Thirty experimental animals were divided into groups as healthy (HG), desflurane control (DCG), TPP and desflurane group (TDG). 20 mg/kg TPP was injected to intraperitoneally TDG. After one hour of TPP administration, desflurane was applied for two hours. After 24 hours, liver tissues of the animals killed with decapitation were removed. The oxidant/antioxidant levels and ALT, AST and LDH activities were measured. The histopathological examinations were performed in the liver tissues for all rats. RESULTS: Notwithstanding the levels of oxidants and liver enzymes were significantly increased (p<0.0001), antioxidant levels were significantly decreased in DCG (p<0.0001). On contrary to the antioxidant parameters were increased (p<0.05) the oxidant parameters and liver enzymes were decreased in TDG (p<0.0001). Whereas multiple prominent, congestion, hemorrhage and dilatation were observed in sinusoids and lymphocyte-rich inflammation results in the centrilobular and portal areas of liver tissue in DCG, these findings were observed less frequently in TDG. CONCLUSİON: Thiamine pyrophosphate prevented liver oxidative damage induced with desflurane and may be useful in prophylaxis of desflurane induced hepatotoxicity.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2016The effect of melatonin on oxidative stress and apoptosis in experimental diabetes mellitus-related ovarian injury
Nayki, Umit; Onk, Didem; Balci, Gurhan; Nayki, Cenk; Onk, Alper; Çankaya, Murat; Taskin Kafa, Ayse Humeyra; Kuzucu, Mehmet
We aimed to evaluate the effect of melatonin on oxidative stress and ovarian injury in rats. Twenty-four Sprague-Dawley albino rats were divided into three groups: Group 1 as nondiabetic healthy controls (n = 8), group 2 as nontreated diabetic rats (n = 8) and group 3 as melatonin-treated diabetic rats (n = 8). After overt diabetes was produced by intraperitoneal injection of streptozosin, 20 mg/kg/day of melatonin was given intraperitoneally to group 3 for a week. NF-kB and caspase-3 immunoexpressions, lipid peroxidation, the activities of antioxidative enzymes, total oxidant capacity and total antioxidant capacity were assessed. Immunoexpressions of NF-kB and caspase-3 were significantly lower in group 3 than group 2. There was a significant decrease in superoxide dismutase activity in group 2 than group 1 and a significant increase in group 3 compared with group 2. We observed a nonsignificant decrease in catalase activity between group 1 and group 2 and a nonsignificant increase between group 2 and group 3. There was a nonsignificant increase in the plasma level of total oxidant status in group 2 than group 1, but a significant decrease was observed in group 3 compared to group 2. Total antioxidant status was significantly lower in group 2 compared with group 1 and group 3. In conclusion, melatonin ameliorates the negative effects of oxidative stress on DM-related ovarian injury.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2016Effect of thiamine pyrophosphate on retinopathy induced by hyperglycemia in rats: A biochemical and pathological evaluation
Cinici, Emine; Ahiskali, Ibrahim; Cetin, Nihal; Suleyman, Bahadir; Kukula, Osman; Altuner, Durdu; Coban, Abdulkadir; Balta, Hilal; Kuzucu, Mehmet; Suleyman, Halis
Purpose: Information is lacking on the protective effects of thiamine pyrophosphate (TPP) against hyperglycemia-induced retinopathy in rats. This study investigated the biochemical and histopathological aspects of the effect of TPP on hyperglycemia-induced retinopathy induced by alloxan in rats. Materials and Methods: The rats were separated into a diabetic TPP-administered group (DTPG), a diabetes control group (DCG) and a healthy group (HG). While the DTPG was given TPP, the DCG and HG were administered distilled water as a solvent at the same concentrations. This procedure was repeated daily for 3 months. At the end of this period, all of the rats were euthanized under thiopental sodium anesthesia, and biochemical and histopathological analyses of the ocular retinal tissues were performed. The results of the DTPG were compared with those of the DCG and HG. Results: TPP prevented hyperglycemia by increasing the amount of malondialdehyde and decreasing endogen antioxidants, including total glutathione, glutathione reductase, glutathione S-transferase and superoxide dismutase. In addition, the amounts of the DNA oxidation product 8-hydroxyguanine were significantly lower in the retinas of the DTPG compared to the DCG. In the retinas of the DCG, there was a marked increase in vascular structures and congestion, in addition to edema. In contrast, little vascularization and edema were observed in the DTPG, and there was no congestion. The results suggest that TPP significantly reduced the degree of hyperglycemia-induced retinopathy. Conclusions: The results of this study indicate that TPP may be useful for prophylaxis against diabetic retinopathy.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2014Four diclofenac complexes with cobalt(II) and nickel(II) ions: Synthesis, spectroscopic properties, thermal decompositions, crystal structures, and carbonic anhydrase activities
Caglar, Sema; Aydemir, Ismihan E.; Cankaya, Murat; Kuzucu, Mehmet; Temel, Ersin; Buyukgungor, Orhan
Cobalt(II) and nickel(II) complexes with diclofenac (dicl) in the presence of nitrogen-donor 3-picoline (3-pic) and 1-(2-aminoethyl)pyrrolidine (2-aepyr), [Co(dicl)2(3-pic)2] (1), [Ni(dicl)2(3-pic) 2(H2O)2] (2), [Co(dicl)2(2-aepyr) 2] (3), and [Ni(dicl)2(2-aepyr)2] (4), have been synthesized and characterized by FT-IR, UV-Vis, elemental and thermal analysis. The crystal structures of 1 and 3 have been determined by single-crystal X-ray diffraction; phase purity of complexes has been proved by powder X-ray diffraction analysis. Structural analyses have demonstrated that 1 and 3 are mononuclear and Co(II) ions have distorted octahedral environments. In 1, dicl is bidentate, whereas in 2, 3, and 4 dicl is monodentate. Dicl ligands are coordinated to metal(II) ions with O of carboxylate. Therefore, IR spectra of all complexes display {(OCO)asym and (OCO)sym} of dicl. The calculated Δ(OCO) values are consistent with the presence of monodentate (>200) and bidentate (<200) carboxylate. Compounds 2, 3, and 4 exhibit inhibition effects on human carbonic anhydrase-I. Compounds 3 and 4 show high thermal stability compared with 1 and 2.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2013Investigation of Some Vitamin Type Inhibition on Human Cord Blood Carbonic Anhydrase I and II
Çetin, Ahmet; Çoban, Taha Abdulkadir; Çankaya, Murat; Nayki, Usmit; Kuzucu, Mehmet
Objective: Human cord blood, a specialized blood tissue, contains many substances including several enzymes which act by different biochemical reactions. Carbonic anhydrase (CA) isoenzymes are one of the most important enzyme family which are crucial for living organisms to survive. However, there is limited data regarding the effect of vitamins on CA isoenzymes. The aim of this study is to test the inhibitory effect of some widely used vitamins on human cord blood CA I and II (hcbCA I and hcbCA II). Methods: We purified hcbCA I and hcbCA II from human cord blood erythrocytes by Sepharose-4B-l-tyrosine- sulfanilamide affinity gel chromatography. The inhibitory effects of vitamin A (retinol), vitamin B12 (cyanocobalamin) and vitamin K (menadione sodium bisulfate, k3) on two isoenzyme were checked using IC50 values. Result: IC50 values for vitamin A, vitamin B12 and vitamin K were found to be 44, 90, and 31 mu M for hcbCA I and of 51, 62, and 21 mu M for hcbCA II; respectively. All these substances were found to be non-competitive inhibitors. Conclusion: Vitamins are vitally important for continuation of life and activity of some of enzymes. While the physicans recommend pregnant women to take vitamins during pregnancy, we suggest that the findings obtained in this study be taken into consideration.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2012Effects of some drugs on human cord blood erythrocyte carbonic anhydrases I and II: An in vitro study
Cankaya, Murat; Aktas, Mehmet; Kuzucu, Mehmet; Gül, Ilhami; Coban, T. Abdulkadir
In the present study, we purified hcbCA I and II from human cord blood erythrocytes using by Sepharose-4B-l tyrosine-sulfanilamide affinity gel chromatography. Also, it was checked the inhibition effects of ampicillin sulfate, ceftriaxone, ceftizoxime and ranitidine on hcbCA I and hcbCA II. IC50 values for ceftriaxone, ceftizoxime and ranitidine were found to be 27.l, 79.4 and 55.5 M for hcbCA I and of 21.0, 79.1 and 66.1 M for hcbCA II, respectively. According to these results, Ampicillin sulfate inhibited only hcbCAII and IC50 values of this antibiotic was found to be 56.8 M. All these substances were found non-competitive inhibitors. It is important to study the inhibition effects of these drugs on hcbCA I and II izoenzymes. Because, pregnant woman is take all of these substance. For this reason, these drugs should be carefully used and the dosage should be very well ordered to minimize side effects.
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