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- ArticleYalnız künye2026Comprehensive Organ-Specific Phenolic Characterization of Endemic Hedysarum candidissimum by Liquid Chromatography–Tandem Mass Spectrometry: Antioxidant, Enzyme-Inhibitory, and Antiproliferative Activities Supported by Chemometric Analysis
Şimşek, Samed; Varol, Tuğçe; Altın, Sevgi; Yılmaz, Mustafa Abdullah; Aydın, Ali; Elveren, Müjgan; Alp, Cemalettin; Köksal, Ekrem; Osma, Etem
Hedysarum candidissimum (HC), a species endemic to Türkiye, represents a potential source of bioactive phytochemicals with pharmacological applications. This study provides the first comprehensive investigation of the species, integrating organ-specific phytochemical profiling with biological activity evaluations. Methanolic extracts from leaf, stem, and root were analyzed for their phytochemical composition and biological activities. Liquid Chromatography–Tandem Mass Spectrometry analysis quantified 53 phenolic compounds and revealed organ-dependent differences in metabolite distribution. Quinic acid was the major phenolic acid, with the highest content in the stem (91.81 mg/g extract), while flavonoid glycosides such as isoquercitrin (90.72 mg/g extract) and astragalin (43.61 mg/g extract) were higher in leaves. Antioxidant activity was determined by DPPH, FRAP, TPC, and TFC assays. The leaf extract showed the strongest activity, with the lowest DPPH IC50 (5.43 ± 0.18 µg/mL), highest reducing power (192.47 ± 0.91 mg TE/g extract), and highest phenolic and flavonoid contents (220.84 ± 1.07 mg GAE/g extract and 107.26 ± 0.77 mg QE/g extract). Enzyme inhibition assays demonstrated strong activity against acetylcholinesterase, butyrylcholinesterase, and α-glucosidase (2.44 ± 0.11, 3.35 ± 0.37, and 3.40 ± 0.20 µg/mL). Antiproliferative activity showed moderate growth inhibition in lung (A549, Calu1, H1650) and breast (MCF-7, MDA-MB-231) cancer cell lines (GI50: 2.40–19.15 µg/mL), with higher sensitivity in breast cells. The stem extract exhibited the highest tumor selectivity index (1.83), while LDH assays indicated low toxicity. Multivariate analyses confirmed organ-specific metabolite patterns and relationships between phenolic composition and biological activities. These findings indicate that the species, particularly the leaf extract, represents a source of phenolic metabolites for further phytochemical and biological investigation.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2026Phenolic Composition, Chemometric Analysis, and Multifunctional Biological Activities of Organ-Specific Extracts From Endemic Hedysarum erythroleucum
Elveren, Müjgan; Varol, Tuğçe; Şimşek, Samed; Aydın, Ali; Yılmaz, Mustafa Abdullah; Altın, Sevgi; Osma, Etem
Hedysarum erythroleucum is a plant species native to Türkiye and represents a potential source of bioactive compounds. This study investigated the phytochemical composition and biological activities of methanolic extracts prepared from the leaves, stems, and roots. LC-MS/MS analysis identified 53 phenolic compounds and revealed clear differences in metabolite distribution among plant organs. Quinic acid was the predominant phenolic acid, particularly in the leaf extract. Flavonoid glycosides such as isoquercitrin, astragalin, and rutin were also abundant in the aerial parts of the plant. The antioxidant potential of the extracts was evaluated using DPPH, FRAP, total phenolic content, and total flavonoid content assays. Among the tested plant parts, the leaf extract exhibited the strongest antioxidant activity, with the lowest DPPH IC50 value, the highest reducing power, and the highest total phenolic and flavonoid contents. These findings indicate that the leaves contain high levels of antioxidant compounds. Enzyme inhibition assays showed that the leaf extract exhibited the highest inhibitory activity against acetylcholinesterase, butyrylcholinesterase, and α-glycosidase. Its activity was comparable to that of the reference inhibitors under the experimental conditions. Antiproliferative activity was assessed against lung and breast cancer cell lines. All extracts inhibited cancer cell growth, with the highest sensitivity observed in H1650 and MDA-MB-231 cells. Tumor selectivity indices suggested moderate selectivity, especially for root extracts. LDH cytotoxicity analysis indicated limited membrane damage, suggesting that the extracts primarily exert cytostatic effects by suppressing cell proliferation rather than inducing extensive cell death. Multivariate analyses revealed organ-specific metabolite patterns and associations between phenolic composition and the measured biological activities. Overall, the findings indicate that H. erythroleucum, particularly the leaf extract, contains a high content of phenolic compounds and exhibits promising in vitro antioxidant, enzyme inhibitory, and antiproliferative activities. These findings provide a basis for future phytochemical investigations, mechanistic studies, compound isolation, and in vivo validation.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2026Enhancing pepper resilience to tebucanozole stress: role of copper and Bacillus subtilis microorganisms
Elveren, Müjgan; Osma, Etem; Varol, Tuğçe; Yaman, Mehmet; Öztürk, Halil İbrahim
Background: In this study, 75 and 100 ppm tebuconazole was applied to pepper seedlings and stress was created. Applications containing Bacillus subtilis, copper and Bacillus subtilis-copper combination were made to reduce the effect of stress on plants. Results: In both tebuconazole doses, significant decreases were observed in plant height, stem diameter, leaf number, leaf area and dry and fresh weights of plants and roots (63%, 37%, 40%, 45%, 27% and 26% respectively). However, bacteria, copper, and combination applications significantly reduced these negative effects; especially, copper-bacteria combination at 75 ppm (K4) and bacteria-only application at 100 ppm (K6) were the most effective in reducing stress. Tebuconazole applications increased oxidative stress and significant increases were detected in catalase (CAT) and superoxide dismutase (SOD) enzyme activities. The highest CAT activity was recorded in the group treated with bacteria (K6) under 100 ppm stress, indicating that antioxidant defenses were strengthened. Photosynthetic pigments (chlorophyll a, chlorophyll b, total chlorophyll and carotenoids) were decreased by tebuconazole stress but were significantly preserved by bacterial and copper treatments. While EC (electrolyte conductivity) value increased due to stress, bacteria and copper applications had a positive effect by decreasing EC values. While tebuconazole stress disrupted the balance of some elements, mineral levels were rebalanced after ameliorative applications, and increases were determined in important elements such as Mn, Mg and B. According to the correlation analysis, significant positive correlations were determined between growth parameters, pigment contents and antioxidant enzyme activities. Conclusions: As a result, Bacillus subtilis and copper applications provided a decrease in toxicity due to stress and increased antioxidant enzyme activity. In addition, ameliorative applications provided protection in photosynthetic pigments and increased cell membrane integrity. These findings indicate that the use of beneficial bacteria such as Bacillus subtilis and micronutrients such as copper (Cu) at appropriate concentrations may be beneficial as sustainable solutions against fungicide stress.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2026First Report on the Phytochemical and Pharmacological Properties of Hedysarum yilmazunalii
Osma, Etem; Varol, Tuğçe; Şimşek, Samed; Altın, Sevgi; Yılmaz, Mustafa Abdullah; Aydın, Ali; Elveren, Müjgan; Kandemir, Ali
This study presents the phytochemical and biological evaluation of the species Hedysarum yilmazunalii Kandemir, F. Yıldız & H.İ. Turkoğlu. Methanolic extracts from leaves, stems, and roots were characterized by LC-MS/MS, and their antioxidant, enzyme inhibitory, and antiproliferative activities were investigated. The analyses showed organ-specific differences in phenolic composition, with quinic acid as the major compound in leaves together with higher levels of flavonoid glycosides, including astragalin and isoquercitrin. Aerial extracts showed strong enzyme inhibitory activity. Leaf and stem extracts exhibited cholinesterase inhibition, with IC50 values of 5.82 and 6.42 µg/mL for AChE and 5.41 and 5.50 µg/mL for BChE, respectively. The leaf extract showed α-glucosidase inhibitory activity (4.10 µg/mL), comparable to acarbose, and antioxidant activity (DPPH: 6.46 µg/mL), together with the highest phenolic (145.43 mg GAE/g extract) and flavonoid contents (87.20 mg QE/g extract). Antiproliferative assays demonstrated growth inhibition of lung and breast cancer cell lines, with the lowest GI50 values of 1.00 µg/mL for H1650 and 2.34 µg/mL for MDA-MB-231 cells. LDH cytotoxicity remained low (2.25%–12.59%), indicating limited membrane damage in both cancer and normal cells. These findings identify H. yilmazunalii as a source of compounds with antioxidant, neuroprotective, antidiabetic, and anticancer potential.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2025EFFECTS OF LIGHT AT DIFFERENT DISTANCES AND POWER ON WHEAT (Triticum aestivum L.) AND BARLEY (Hordeum vulgare L.) GROWTH
Aricioğlu, Ayşegül; Akaltun, Yunus; Elveren, Müjgan; Osma, Etem
Global population growth increases the need for efficient methods of maintaining a stable food supply. Light is one of the most important influences on plant growth and development. Artificial lighting elements used in agricultural applications have different effects on plants. This study analyzed the effects of metal halide (MH) lamps with a wavelength of 450 nm, at distances of 1, 1.5, and 2.5 m, on barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.) growth. Growing occurred in light magnification rooms without daylight, using 250, 400, and 600 W MH lamps as the only light source. Plants exposed to light for 13–14 h a day were harvested after 18 days. After harvest, the amount of electrolyte leakage, chlorophyll, carotenoid, and B, Cu, Mn, Zn, K, and Ca intake were determined using inductively coupled plasma optical emission spectroscopy (ICP-OES). The data were analyzed with the SPSS 22 Statistical Package program. Wheat and barley grown at different light distances and intensities showed statistically significant changes in mineral element uptake and plant chlorophyll levels. This research contributes to the optimization of agricultural productivity and the understanding of the interaction between light quality and other environmental parameters.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2025Antioxidant and antimicrobial activities of plant flowers growing in distinct geological habitats (serpentine, gypsum, and limestone)
Şimşek, Samed; Varol, Tuğçe; Akşit, Zeynep; Elveren, Müjgan; Osma, Etem
In this study, the flowers of 20 plant species [Gypsum (Linum flavum, Paracaryum stenolophum, Onobrychis nitida, Gypsophila lepidioides, Psephellus recepii, Tanacetum heterotomum, Verbascum alyssifolium, limestone (Teucrium leucophyllum, Stachys sparsipilosa, Phlomis oppositiflora, Pelargonium endlicherianum, Chrysophthalmum montanum, serpentine (Convolvulus pseudoscammania, Fumana aciphylla, Gladiolus halophilus, Hypericum thymbrifolium, Salvia indica, Artedia squamata, Erysimum pulchellum, Consolida olopetala], including 11 endemic species, growing in various habitats (gypsum, limestone, and serpentine) in Erzincan province were examined. By focusing on the antioxidant capacities and antibacterial activities of these species, the study aims to elucidate the effects of these ecosystems on the biosynthesis of plant secondary metabolites, primarily phenolic compounds and flavonoids, and their adaptive biochemical responses to unique environmental conditions. Antioxidant properties were assessed using DPPH, FRAP, TPC, and TFC assays. Results showed significant differences in antioxidant compound concentrations among plants from different habitats, with the highest levels in serpentine and gypsum regions. Artedia squamata L. from serpentine had the strongest DPPH activity (24.83 ± 1.25 µg/ml), Erysimum pulchellum had the highest TPC (89.44 ± 0.76 mg GAE/g), and Tanacetum heterotomum had the highest TFC (43.48 ± 2.08 mg QE/g) in gypsum. FRAP activity was highest in serpentine species, particularly Consolida olopetala (32.14 ± 1.84 mg TE/g). Furthermore, Antibacterial assays against eight ATTC strains revealed significant inhibitory effects by Stachys sparsipilosa, Tanacetum heterotomum, Teucrium leucophyllum, and Verbascum alyssifolium against Enterococcus faecium and Salmonella enteritidis with Teucrium leucophyllum showing the highest activity (MIC of 31.25 µg/ml against Salmonella enteritidis). The study highlights the influence of habitat on the biosynthesis of bioactive compounds, suggesting that plants from serpentine and gypsum soils have enhanced antioxidant and antimicrobial properties, making them potential candidates for natural therapeutic agents.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2024Erzincan İli-Ergan Dağı’nın Farklı Rakımlarında Yayılış Gösteren Bazı Taksonların Antioksidan Kapasiteleri
Kandemir, Ali; Osma, Etem; Varol, Tuğçe; Şimşek, Samed; Adıgüzelli, Gökhan
Bu çalışmada, Erzincan il sınırları içerisinde bulunan Ergan Dağı’nın farklı rakımlarında 1500 m (Glaucium leiocarpum Boiss., Astragalus ornithopodioides Lam., Alcea calvertii (Boiss) Boiss.), 2000 m (Globularia trichosantha Fisch. & C.A. Mey, Hypericum scabrum L., Tanacetum mucroniferum Hub. Mor. Et Grierson, Inula oculus-christi L., Onobrychis cornuta (L.) Desv., Barbarea auriculata Hausskn. Ex Bornm), 3000 m (Scorzonera sericea DC., Hedysarum erythroleucum Boiss., Astragalus nezaketiae A. Duran & Aytaç) yayılış gösteren 12 bitki türü seçilmiştir. Çalışma ile rakım faktörünün bitkiler üzerindeki etkileri belirlenmesi amaçlanmıştır. Bunun için bitkilerin yaprak, dal ve kök olmak üzere farklı organları etilasetat, metanol ve suda ekstaksiyonu yapılarak fenolik (TPC) ve flavonoid (TPF) içeriği ile antioksidan aktivite (DPPH, FRAP) miktarının analizi yapılmıştır. Antioksidan aktiviteleri incelendiğinde, Globularia trichosantha bitki türünde en yüksek değerler belirlenmiştir. DPPH aktivitesi yaprakta 47,86±1,70, dalda 108,09±1,29, kökte ise 60,57±0,74 olarak ölçülmüştür. Fenolik içerik ise yaprakta 82,56±0,46, dalda 56,71±2,12, kökte ise 59,75±2,11 olarak tespit edilmiştir. FRAP aktivitesi yaprakta 54,87±1,54, dalda 41,09±3,06, kökte ise 48,95±2,73 olarak belirlenmiştir. Flavonoid içerik verileri ise Globularia trichosantha yaprakta 59,81±2,31, dalda 31,62±3,55 olarak tespit edilirken, Hypericum scabrum kökte 35,71±2,73 olarak belirlenmiştir. Analiz sonuçları SPSS 22 Paket İstatistik Programında değerlendirilmiş olup rakımlar (yükselti) arasında farklılıklar tespit edilmiştir. Bitkilerin fenolik ve flavonoidlerin içerikleri ile antioksidan aktiviteleri arasında özellikle metanolde çözünen örneklerde güçlü pozitif yönde korelasyon bulunmuştur. Sonuç olarak, bu çalışma farklı rakımlarda yetişen bitkilerin fenolik ve flavonoid içeriği ile antioksidan aktivitelerindeki değişkenliği açıklamaktadır. Bulgular, rakımın bu bitki türlerinin biyoaktif bileşenleri ve antioksidan potansiyeli üzerindeki etkisini vurgulamaktadır. Ayrıca, 2000 m rakımda yetişen Hypericum scabrum L., Tanacetum mucroniferum Hub. Mor. Et Grierson, Globularia trichosantha Fisch.&C.A.Mey bitkilerinin gösterdiği antioksidan performansı, bu bitkilerin farmasötik veya diğer uygulamalarda daha fazla keşif için potansiyel oluşturmaktadır.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Erzincan İli-Ergan Dağı'nda Yaygın Bitki Türleri ve Topraklarındaki Mineral Elementlerin Rakımsal Değişimi
Elverici, Mert; Kandemir, Ali; Kılıç, Engin; Osma, Etem; Varol, Tuğçe; Yildiz, Faruk; Türkoğlu, Halil İbrahim; Hasbenli, Abdullah; Kiymaz, Muhammet Onur
Bu çalışma, 2021-2022 yıllarında Erzincan il sınırları içerisinde yer alan Ergan Dağı’nın farklı yüksekliklerinde yetişen 30 bitki türü ile yapılmıştır. Bunun için 1500 m yükseklikte (Glaucium leiocarpum Boiss., Alcea calvertii, Genista aucheri, Astragalus ornithopodioides, Anchusa leptophylla, Linum mucronatum, Euphorbia virgata) 2000 m’de (Genista aucheri, Hypericum scabrum, Tanacetum mucroniferum, Barbarea auriculata, Onobrychis cornuta, Inula oculus-christi, Euphorbia virgata, Globularia trichosantha) 2500 m’de (Aster alpinus, Lallemantia canescens, Scutellaria orientalis, Dianthus orientalis, Coronilla orientalis, Campanula tridentata, Crepis armena, Anthemis cretica, Euphorbia petrophila, Papaver fugax) ve 3000 m’de (Senecio hypoleucus, Pedicularis comosa, Scorzonera sericea, Anthemis cretica, Astragalus nezaketiae, Papaver fugax, Campanula tridentata, Senecio hypoleucus, Hedysarum erythroleucum) olacak şekilde farklı rakımlardan toplanan bitkiler ve yetiştikleri topraklarda mineral elementlerin konsantrasyonu belirlenmiştir. Toplanan bitki (kök, yaprak, çiçek) ve toprak örnekleri ön işlemlerden geçirildikten sonra ICP-MS cihazında analiz edilmiştir. Elde edilen veriler istatistiksel olarak değerlendirilerek, rakımlar arasındaki farklılıklar olduğu tespit edilmiştir. Yükselti arttıkça topraktaki elementlerin genel olarak azaldığı görülmüştür.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023Ergan Dağı’nın Farklı Yükseltilerinde Yaygın Olarak Yetişen Bitkilerde Ağır Metal (Ba, Cd, Co, Cr, Pb) Birikiminin Belirlenmesi
Elverici, Mert; Kandemir, Ali; Kılıç, Engin; Osma, Etem; Varol, Tuğçe; Yildiz, Faruk; Türkoğlu, Halil İbrahim; Hasbenli, Abdullah; Kiymaz, Muhammet Onur; Akverdi, Aysel
Bu çalışma, Erzincan il sınırları içerisinde yer alan Ergan Dağı’nın farklı yüksekliklerinde yetişen yaygın 30 bitki türü ile yapılmıştır. Çalışma kapsamında 1500 m, 2000 m, 2500 m ve 3000 m olmak üzere farklı yüksekliklerden toplanan bitkilerde ve yetiştikleri topraklarda metal (Cd, Cr, Pb, Co, Ba) konsantrasyonları tespit edilmiştir. Toplanan bitkilerin çiçek, yaprak ve kök ile yetiştikleri topraklar laboratuvarda ön işlemlerden geçirilmiştir. Hazır hale getirilen örneklerde metal konsantrasyonları ICP-MS’te analizi gerçekleştirilmiştir. Elde edilen bitki ve toprağa ait metal verileri istatistiksel olarak değerlendirilerek, her bir metal için rakımlar arasındaki farklılıklar belirlenmiştir. Çalışmada Cd ve Pb konsantrasyonunun 2500 m yetişen bitkilerde ve topraklarda yüksek olduğu belirlenmiştir. İlave olarak, Turanecio hypochionaeus ve Crepis armena türlerinin bünyesinde kadmiyum metalini toprağa göre daha fazla biriktirdiği ve bazı türlerin biyomonitor özelliğe sahip olduğu anlaşılmıştır.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023Farklı Habitatlarda (Jips, Kireçtaşı, Serpentin) Yetişen Bitkilerde Bazı Ağır Metallerin (Ba, Cd, Co, Cr, Pb) Konsantrasyonunun Belirlenmesi
Osma, Etem; Varol, Tuğçe; Elveren, Müjgan
Bu çalışmada, Erzincan il sınırları içerisinde farklı habitatlarda (jips, kireçtaşı, serpentin) yetişen 25 bitki türü Jips (Ebenus macrophylla Jaub and Spach., Gypsophila lepidioides Boiss., Linum flavum L., Onobrychis nitida Boiss., Paracaryum stenolophum Boiss., Psephellus recepii Wagenitz & Kandemir, Tanacetum heterotomum (Bornm.) Grierson, Verbascum alyssifolium Boiss.), kireçtaşı (Alkanna megacarpa DC., Alyssum sibiricum Willd., Anthemis sp., Astragalus sp., Chrysophthalmum montanum (DC.) Boiss, Ebenus laguroides Boiss., Genista aucheri Boiss., Pelargonium endlicherianum Fenzl., Stachys sparsipilosa R.Bhattacharjee & Hub-Mor.) serpantin (Artedia squamata L., Consolida sp., Convolvulus pseudoscammania C. Koch., Erysimum pulchellum Willd., Fumana aciphylla Boiss., Gladiolus halophilus Boiss.& Heldr., Hypericum thymbrifolium Boiss & Noé, Salvia indica L.) ile çalışılmıştır. Çalışma kapsamında farklı bitkilerde ve yetiştikleri topraklarda metal (Cd, Cr, Pb, Co, Ba) konsantrasyonları belirlenmiştir. Toplanan bitkilerin çiçek, yaprak ve kök kısımları ile yetiştikleri habitatların toprakları laboratuvarda ön işlemlerden geçirilmiştir. Sonrasında hazırlanan örneklerde metal konsantrasyonu ICP-MS’ te analiz edilmiştir. Elde edilen bitki ve toprak verileri istatistiksel olarak değerlendirilerek, habitatlar arasındaki farklılıklar belirlenmiştir. Bitkinin generatif ve vegetatif kısımlarında metal bakımından farklılıklar olduğu tespit edilmiştir.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023Kaya Tuzu ile Deniz Tuzu Stresinin Buğday (Triticum vulgare L.) ve Arpa (Hordeum vulgare L.) Üzerindeki Etkileri
Osma, Etem; Varol, Tuğçe; Elveren, Müjgan; Kara, Ali
Bu çalışmada kaya tuzu ile deniz tuzunun buğday ve arpa gelişimi üzerindeki etkileri kıyaslanmıştır. Çalışma için 650 g toprağa 4 g arpa ve 5 g buğday tohumu ekilerek üzerleri 100 g toprak ile kaplanmıştır. Çimlenen buğday ve arpalara 50, 100 ve 250 NM olacak şekilde farklı konsantrasyonlarda bir kez kaya tuzu ile deniz tuzu çözeltisi verilmiştir. Bitkiler yetiştirilme sürecinde tarla kapasitesine uygun sulanarak, 15 gün yetiştirildikten sonra hasat edilmiştir. Hasadı yapılan örneklerde elektrolit sızıntı miktarı ile antioksidan aktiviteleri (CAT, SOD, POD) belirlenmiştir. Kontrol örnekleri ile deniz ve kaya tuzunun uygulandığı örnekler arasındaki ilişki SPSS 22 İstatistik Paket Programı’nda istatistiksel olarak değerlendirilmiştir. Örnekler arasında anlamlı farklılıklar olduğu tespit edilmiştir. Sonuç olarak, farklı tuz konsantrasyonlarının uygulandığı örneklerde konsantrasyon artışına bağlı olarak bitkilerin elektrolit sızıntı ile antioksidan aktivitelerinde önemli ölçüde artış gözlenmiştir.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023Antioxidant activities of plant species growing in different habitats (serpentine, gypsum and limestone)
Osma, Etem; Varol, Tuğçe; Elveren, Müjgan; Şimşek, Samed
In this study, plant species (gypsum, limestone, and serpentine) growing in different habitats in Erzincan province were investigated. Gypsum [Verbascum alyssifolium Boiss., Tanacetum heterotomum (Bornm.) Grierson, Psephellus recepii Wagenitz & Kandemir, Gypsophila lepidioides Boiss.], limestone [Cyclotrichium niveum (Boiss.) Manden. & Scheng, Chrysophthalmum montanum (DC.) Boiss, Teucrium leucophyllum Montbret & Aucher ex Bentham, Phlomis oppositiflora Boiss. & Hausskn] serpentine [Fumana aciphylla Boiss., Convolvulus pseudoscammania C. Koch., Hypericum thymbrifolium Boiss & Noé, Salvia indica L., Gladiolus halophilus Boiss. & Heldr.] were examined. The total phenolic contents (TPC), total flavonoid content (TFC), and the amount of antioxidant activity (DPPH, FRAP) were analyzed in different organs of the plants, including leaves, branches, and roots. According to the obtained data, when plant parts and habitats were taken into consideration, it was observed that the plant with the highest flavonoid content (29.71±0.57 mg QE g-1 extract) was S. indica growing in the serpentine area with its leaf parts. In terms of total phenolic content, it was determined that the root part of S. indica growing in the serpentine area had high values (91.53±2.48 mg GAE g-1 extract value). When evaluated in terms of the Iron (III) Ion Reducing Antioxidant Power (FRAP) method, it was observed that the highest value was the stem part of F. aciphylla growing in a serpentine area (100.35±1.60 mg TE g-1). In terms of DPPH radical capacity, the highest value belonged to the leaf part of Salvia indica (15.75±1.74 μg mL-1), which is also grown in the serpentine area. The results were evaluated utilizing the SPSS Statistical Program and differences were observed between habitats. A strong correlation was found between the phenolic and flavonoid contents of plants and their antioxidant activities. The findings showed that the phenolic, flavonoid content, and antioxidant activities of plants grown in different ecological conditions vary significantly.
RDL Kalıcı Kaydıİncele - ArticleYayıncıda açık erişim2023Investigation of the effects of mineral elements on flower coloration of plants growing in different habitats (limestone, serpentine, gypsum)
Osma, Etem; Varol, Tuğçe; Elveren, Müjgan
In this study, 25 plant species growing in different habitats in Erzincan province were studied with gypsum (Ebenus macrophylla, Gypsophila lepidioides, Linum flavum, Onobrychis nitida, Paracaryum stenolophum, Psephellus recepii, Tanacetum heterotomum, Verbascum alyssifolium), limestone (Alkanna megacarpa, Alyssum sibiricum, Anthemis sp., Astragalus sp., Chrysophthalmum montanum, Ebenus laguroides, Genista aucheri, Pelargonium endlicherianum, Stachys sparsipilosa), or serpentine (Artedia squamata, Consolida olopetala, Convolvulus pseudoscammania, Erysimum pulchellum, Fumana aciphylla, Gladiolus halophilus, Hypericum thymbrifolium, Salvia indica). Within the scope of the study, element concentrations were determined in plants with different flower colors (yellow, purple–blue–pink, white) and in the soils where they grow. Our goal was to assess whether there were differences in mineral element concentrations within plants from these different areas and whether those differences (if they existed) led to changes in plant morphology, specifically flower coloration. The flowers, leaves and root parts of the collected plants and the soils of the habitats where they grow were pre-treated in the laboratory. Mineral element concentrations in the prepared samples were determined by ICP-MS. Plant and soil data obtained were statistically evaluated, and the differences between the habitats were determined. It was observed that there were differences in terms of mineral elements in the generative and vegetative parts of the plant and these differences impacted flower color; these coloration differences in plants from other areas might be used to indicate the presence of potentially important minerals.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2022The Effects of LEDs with different CCT values on growth characteristics of Triticum aestivum L. (wheat) and Hordeum vulgare L. (barley)
Osma, Etem; Elveren, Müjgan; Karagöz, Zehra; Sahin, Mustafa; Akaltun, Yunus
Light Emitting Diode (LED)s are used extensively in almost everywhere in our daily life and they have different color temperatures such as Correlated Color Temperature (CCT) which is represented by ˚K (Kelvin). In this study, wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) were cultivated in laboratory environment under LED with different CCT values (2000, 3000, and 6000˚K). After cultivation, plant height and weight values, the quantity of chlorophyll and carotene, the amount of leakage of electrolyte and element absorption capacity from the soil, POX (Peroxidase) and SOD (Superoxide Dismutase) enzyme activities of cultivated plants were determined. Results were evaluated on Statistical Package for the Social Sciences Program (SPSS 22) and significant differences were obtained on plants which were grown at different color temperatures of light. It was concluded that measurements from plants which were grown under LED light with cold color temperatures (6000˚K) were more consistent than those of plants grown under other lights with different temperatures. It has been deduced that cold color temperatures are closer to the optimum light values required for a plant to grow faster.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2022Farklı Şekillerde Üretilen Işığın Triticum aestivum L. (Buğday) ve Hordeum vulgare L. (Arpa) Üzerindeki Etkileri
Osma, Etem; Sahin, Mustafa; Kılıç, Elçin
Tarımsal uygulamada kullanılan farklı aydınlatma elemanları ile farklı niteliklerde ışıklar üretmektedir. Bu durum canlılar üzerinde farklı etkiler oluşturmaktadır. Bu çalışmada, aydınlatma uygulamalarında ışık üretim yöntemlerinden en fazla kullanılan termik, deşarja dayanan ve elektrolüminesans yol ile üretilen ışığın Triticum aestivum L. (Buğday) ve Hordeum vulgare L. (Arpa) üzerindeki etkisi incelenmiştir. Bu amaçla, LED, enkandesan, sodyum buharlı, civa buharlı ve metal halojenli deşarj ışık kaynaklarının kullanıldığı farklı aydınlatma ortamları oluşturulmuş ve ışık kaynağı haricinde diğer tüm değişkenlerin aynı olması sağlanmıştır. Sonrasında bu ortamlarda buğday ve arpa bitkileri yetiştirilmiş ve 15 gün sonunda hasat edilmiştir. Hasat işlemleri tamamlandıktan sonra bitkilerin yaş ağırlıkları, boyları, elektrolit sızıntı, klorofil ile karoten miktarları, Süperoksit dismutaz (SOD) ve Katalaz (CAT) enzim aktiviteleri belirlenmiştir. Elde edilen veriler SPSS İstatistik Paket Programı’nda değerlendirilerek çalışılan ışık parametreleri altında yetiştirilen bitkiler arasındaki farklılıklar belirlenmiştir. Farklı lambalar altında yetiştirilen buğday ve arpalarda elde edilen veriler arasında istatistiksel olarak anlamlı farklılıklar olduğu gözlenmiştir.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2022MIXTURES of PHARMACEUTICALS and PERSONAL CARE PRODUCTS (PPCPs) EFFECT PLANT STRESS MARKERS and NUTRIENT UPTAKE in WHEAT and BARLEY
Osma, Etem; Elveren, Müjgan
Common drug active substances (gemfibrozil, β-estradiol, caffeine, and ciprofloxacin) were combined in simple mixtures and applied to soil containing wheat or barley. Mixtures in water (50 μg/mL) were applied twice during the growing period. Harvested plant samples were extracted and antioxidant enzyme activities, as indicated by CAT, POD, and SOD were compared in exposed and control plants. Lipid peroxidation markers were also determined. Finally, mineral element uptake in exposed and control plants was determined. The data indicated differences in several, if not all, of the plant biomarkers between control plants and those treated with simple mixtures of pharmaceutical substances; many were directly related to oxidative damage. It was determined that there were statistically significant differences in the element intake of 8 nutrients (magnesium, potassium, phosphorus, calcium, zinc, mangan, copper, and iron) in control plants compared with plants treated with PPCPs; we observed both increases and decreases in plant nutrients depending on the particular nutrient, pharmaceutical treatment, and plant species.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2022EFFECTS OF PHARMACEUTICALS AND PERSONAL CARE PRODUCTS (PPCPs) IN WATER ON WHEAT (Triticum aestivum L.)
Elveren, Müjgan; Osma, Etem
The present research attempts to fill data gaps in our knowledge of the potential negative impacts of pharmaceuticals and personal care products (PPCPs)6 in water on plants. Ciprofloxacin, doxylamine succinate, and ibuprofen were used as model PPCPs in trials assessing their impact on wheat (Triticum aestivum). Seed germination and growth, electrolyte leakage, and several other plant physiology markers were used as endpoints to assess plant stress over time and at varying PPCPs exposure scenarios/concentrations. Nearly all of the physiological parameters and antioxidant enzyme activities measured were lowest in control (untreated) plants. It was also observed that PPCPs applied at different concentrations to soil increased H2O2, peroxidase (POX), catalase (CAT), and superoxide dismutase (SOD) in plants depending on the exposure concentration as well as time.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2021Farklı Özellikteki Tencerelerde Pişirilen Sebzelerde Element Birikiminin Araştırılması
Osma, Etem; Elveren, Müjgan
Bu çalışmada, mutfaklarda yemek pişirmede kullanılan tencerelerden pişirme esnasında gıdalara bulaşabilecek elementler ile canlılar için gerekli mineral elementlerin konsantrasyonu tespit edilmiştir. Bunun için çelik, bakır, teflon, granit, alüminyum ve döküm tencerelerde pişirilmiş sebzeler patlıcan (Solanum melongena L.), taze fasulye (Phaseolus vulgaris L.), pırasa (Allium ampeloprasum L.) ile çiğ sebzelerde element konsantrasyonları ICP- OES’te analiz edilmiştir. Pişirilmiş ve çiğ sebzelere ait element verileri istatistiksel olarak SPSS 22 İstatistik Paket Programı’nda değerlendirilmiştir. Çiğ ve farklı tencerelerde pişirilen sebzeler arasında güçlü yönde anlamlı farklılıkların olduğu görülmüştür. Bakır tencerelerde Cu, Aliminyum tencerelerde Al konsantrasyonunun genelde yüksek olduğu tespit edilmiştir. Mg, Ca, K ve P gibi canlıların makro düzeyde ihtiyaç duyduğu mineral elementlerin konsantrasyonunun genelde çiğ sebzelerde daha yüksek olduğu belirlenmiştir. Sonuç olarak; pişirme araçlarının besinler üzerinde etkili olduğu ve mutfakta kullanılan ekipmanların insan sağlığı üzerinde oluşturabileceği etkiler dikkate alınarak gerekli önlemlerin alınması gerektiği anlaşılmıştır.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2020Investigation of Heavy Metal Accumulation in (Pinus nigra Arnold) Collected from Different Regions of Ankara
Osma, Etem; Türk, Hasan Can
In this study, heavy metal concentrations (Al, Cr, Cd, Cu, Fe, Zn, Mn, Pb, Ni) were detected in the samples of bark, leaf and soil belonging to P. nigra which were collected from industry, inner city, roadside and control area in Ankara. The aim of this study is to determine the level of heavy metal pollution in Ankara that capital our country. After plant and soil samples were preprocessed, the analyses of heavy metals were performed on ICP-OES. Data obtained from the parts of the plant and the soil was statistically evaluated on SPSS and it is detected that there are significant differences between the regions. It is also observed that there are differences between washed and unwashed leaves in terms of heavy metal concentrations. When the data obtained were examined, it was determined that the concentration of heavy metals in plant barks, especially in the industrial zone, was high. Consequently, it was concluded that P. nigra’s which are widely distributed in cities, may be biomonitors in detecting heavy metal pollution.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2020Exposure to pharmaceuticals affects nutrient and metal uptake by wheat, triticum aestivum L.
Osma, Etem; Elveren, Müjgan
Interest in water recycling has provided opportunities to evaluate the potential impact of pharmaceuticals and personal care products (PPCPs) on plants where recycled wastewater is used for watering and irrigation. Most researches have focused on the impact of pharmaceuticals on seed germination and plant physiology or the uptake of pharmaceuticals into plants. We sought to find out whether nutrient and metal uptake into wheat plants were affected by the presence of PPCPs (acetaminophen, caffeine, β-estradiol, and gemfibrozil) in soil. As acetaminophen, caffeine, β-estradiol, and gemfibrozil concentrations in soil increased, plant physiological parameters such as electrolyte leakage and lipid peroxidation also increased significantly over levels in control plants grown in parallel. Furthermore, increasing PPCP soil concentrations led to decreasing element concentrations in wheat plants, suggesting a reduction in uptake of nutrients and metals in the presence of pharmaceuticals. Our short-term observations suggest plants irrigated with recycled wastewater containing PPCPs experience stress which is ultimately reflected in a reduction of nutrient and metal uptake by wheat plants.
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