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- ArticleYalnız künye2026Enhanced wheat (Triticum aestivum) performance: effects of PGPR-enriched on yield and biochemical profiles
Yildiran Sogurtlupinar, Sevda; Inanç, Merve; Keçe, Yusuf Murat; Tarhan, Leyla; Sogutmaz Ozdemir, Bahar; Sahin, Fikrettin; Turan, Metin; Günes, Adem
Plant growth-promoting rhizobacteria (PGPR) are increasingly applied in sustainable agriculture to enhance plant growth, improve yield, and induce tolerance to stress condition while reducing chemical inputs. Tuff, a porous volcanic material with high water-holding and cation exchange capacity, was used as a carrier to inoculate and deliver PGPR to the plant root zone. This two-year greenhouse study evaluated the effects of PGPR-inoculated tuff (T1) compared with non-inoculated tuff (T0, control) on the growth, physiological performance, antioxidant enzyme activities, and biochemical profiles of 'K & imath;rik' wheat. Large tuff (9 & times; 14 & times; 11 mm) were inoculated with approximately 4.8 & times; 1010 cfu/g PGPR and applied to the root zone of each plant. PGPR application increased grain yield by 20.43% and significantly improved chlorophyll content, stomatal conductance, membrane permeability, and photosynthetic rate. Antioxidant enzymes, including CAT, SOD, and POD, showed increases of 11.43, 14.83, and 10.71%, respectively. Levels of IAA (13.59%), GA (7%), and SA (9.27%) were elevated, whereas ABA decreased by 16.67%. Amino acid and organic acid contents were generally enhanced, along with significant increases in nutrient element concentrations, most notably P (18.49%), Cu (10.61%), and Fe (9.99%); in contrast, Na and B concentrations decreased by 5 and 3.48%, respectively. These findings indicate that PGPR-inoculated tuff can improve nutrient availability and growth and physiological performance in wheat, offering a low-cost, scalable biological input to complement conventional fertilization in sustainable wheat production. Further field studies comparing this approach with standard chemical fertilization are needed to confirm its practical applicability and support broader adoption.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2026Impact of PGPR-enriched tuff on biochemical characteristics, antioxidant enzyme activities and hormonal responses in walnut saplings
Yıldıran Söğürtlüpınar, Sevda; İnanç, Merve; Keçe, Yusuf Murat; Tarhan Çelebi, Leyla; Soğutmaz Özdemir, Bahar; Şahin, Fikrettin; Turan, Metin; Güneş, Adem
Walnut (Juglans regia L.) is one of the most economically important tree nuts worldwide due to its nutritional and health-promoting properties. The use of plant growth-promoting rhizobacteria (PGPR) in agriculture is increasing attention as a sustainable alternative to intensively used chemical inputs; however, their application to walnut seedlings and their effects on changes in plant hormones, antioxidant enzyme activities and biochemical metabolites are not yet fully understood. Therefore, this study aims to investigate the effects of PGPR-enriched tuff on the hormonal responses and metabolite profiles of walnut seedlings. The experiment was conducted for two consecutive years in a greenhouse using the ‘Şebin’ cultivar. Medium-sized tuff pieces (8 × 8 × 11 mm) were inoculated with approximately 2.9 × 109 colony-forming units (cfu) g−1 PGPR applied directly to the root zone of each seedling. The results of the experiment showed that the application of tuff enriched with PGPR significantly improved physiological and biochemical properties compared to the control group. Significant increases were recorded in chlorophyll content, stomatal conductance, membrane permeability, and photosynthetic rate. Antioxidant enzyme activities and concentrations of key plant hormones including indole acetic acid (IAA), gibberellic acid (GA), and salicylic acid (SA) reached their maximum levels under PGPR treatment, while abscisic acid (ABA) showed the highest accumulation in the control. Amino acid and organic acid contents of walnut leaves generally increased with PGPR application. Most nutrient element concentrations were enhanced. PGPR-inoculated tuff demonstrated strong potential as a sustainable soil amendment to improve walnut seedling nutrient uptake, physiological performance, and stress tolerance.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2025Composition of Anthocyanins, Specific Sugars, and Organic Acids in Wild Edible Aromatic and Medicinal Vegetables
Yildirim, Ertan; Turan, Metin; Ekinci, Melek; Ercisli, Sezai; Ozturk, Halil Ibrahim; Aydin, Murat; Ilhan, Emre; Vicas, Simona Ioana; Iancu, Carmen Violeta; Gitea, Daniela
This study investigates the bioactive compound profiles of 12 wild edible plant species from the Amaranthaceae, Apiaceae, Asteraceae, Brassicaceae, Caryophyllaceae, Lamiaceae, Malvaceae, Polygonaceae, and Urticaceae families, consumed as aromatic and medicinal vegetables by local communities and forming part of the natural vegetation of Mount Ergan. The plants were collected and analyzed for their anthocyanin, organic acid, and sugar contents, using advanced liquid chromatography techniques. Statistically significant differences were observed between species for each compound analyzed, highlighting their diverse phytochemical profiles. Malva neglecta, Brassica nigra, and Taraxacum phaleratum exhibited the highest anthocyanin contents, suggesting their strong potential as natural antioxidant sources. Organic acid levels were notably elevated in Polygonum cognatum, T. phaleratum, Urtica dioica, and M. neglecta, which positions these species as promising candidates for use as natural acid regulators in food and pharmaceutical formulations. In terms of sugar content, Chenopodium album, Mentha longifolia, and T. phaleratum had the lowest levels, while M. neglecta, Cirsium arvense, P. cognatum, and Tragopogon buphthalmoides showed significantly higher concentrations, indicating potential applications in the development of natural sweeteners. This study’s findings provide valuable insights into the phytochemical diversity of these wild plant species, emphasizing their potential utility in health nutrition, pharmaceuticals, and cosmetics. This study emphasizes the significance of investigating underexploited plant species for their bioactive chemicals and illustrates their potential contribution to the development of sustainable, natural product-based solutions for diverse industrial uses.
RDL Kalıcı Kaydıİncele - CorrectionAçık Erişim2025Correction to: Essential oils combat Botrytis cinerea in apples: unveiling sugar, vitamin, and antioxidant dynamics (Journal of Food Measurement and Characterization, (2024), 18, 7, (5590-5601), 10.1007/s11694-024-02590-y)
Karakus, Sinem; Atıcı, Okkes; Turan, Metin; Kaya, Ozkan
In this article, the second reference ‘A. Muder, H. Garming, S. DreisiebnerLanz, K. Kerngast, F. Rosner, K. Klickova, G. Kurthy, K. Cimer, A. Bertazzoli, V. Altamura, G. De Ros, K.M.B.C. Zmarlicki Freitas, A. Duarte, E. Bravin, J. Kambor, D. Karamürsel, F.P. Öztürk, E. Kaçal, Apple production and apple value chains in Europe. Eur. J. Hortic. Sci. 1611–4434 (2022)’ was incorrectly published and should have been ‘A. Muder, H. Garming, S. DreisiebnerLanz, K. Kerngast, F. Rosner, K. Klickova, G. Kurthy, K. Cimer, A. Bertazzoli, V. Altamura, G. De Ros, K.M.B.C. Zmarlicki Freitas, A. Duarte, E. Bravin, J. Kambor, D. Karamürsel, F.P. Öztürk, E. Kaçal, Apple production and apple value chains in Europe. Eur. J. Hortic. Sci. 87(6), 1–22 (2022). https://doi.org/10.17660/ejhs.2022/059’. The original article has been corrected.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Essential oils combat Botrytis cinerea in apples: unveiling sugar, vitamin, and antioxidant dynamics
Karakus, Sinem; Atıcı, Okkes; Turan, Metin; Kaya, Ozkan
This study investigated the effects of individual and combination applications of EOCs against Botrytis Cinerea in apple fruits, with a focus on sugar, vitamin, and antioxidant enzyme dynamics. In protective and curative treatments, sucrose content varied between 18.9 and 87.9 g.L-1, with the “CT” group exhibiting significantly higher sucrose levels (87.9 g.L-1). Glucose content ranged from 6.2 g.L-1 (“CT” and “C + F”) to 35.5 g.L-1 (“T + C + E + F”). Fructose content varied significantly, with the “F + T + C + E” treatment showing the highest (11.4 g.L-1) and the “CT” group the lowest (2.5 g.L-1). Rhamnose content in the curative treatment reached 14.9 g.L-1 (“F + T”) and in the protective treatment, the “C + E + F” group had the highest (3.3 g.L-1). Galactose content ranged from 0.1 to 13.0 g.L-1. Xylose content in curative treatment ranged from 12.1 to 13.6 g.L-1, with the combination “F + T” displaying the highest. In preventive treatment, arabinose content ranged from 0.7 to 3.0 g.L-1, with the “T + C + F” group having the highest. In terms of vitamins, the combination “T + F” showed the highest B1 (25.4 mg.100 g-1) and B2 (24.6 mg.100 g-1) content. Vitamin A, B1, and B2 increased in the “F + T” group, and vitamin C increased in the “F + T + C” group (33.4 mg.100 g-1). The quantification of oxidative stress markers and antioxidant enzymes revealed significant reductions in H2O2, MDA, and proline levels in EOC applications. In preventive treatment, the combination “T + C + E + F” exhibited the most effective improvement in SOD activity (556.5 EU mg-1), CAT activity increased significantly in the “T + C + F” group (189.3 EU mg-1), and APX activity saw the highest increase in the “T + C + E + F” group (185.6 EU mg-1). Contrastingly, curative treatments showed higher H2O2, MDA, and proline levels. The most significant decrease in H2O2 level occurred in the “T + C + E + F” group (36.7 nmol.g-1), and a significant decrease in MDA level was noted in the same group. Notable changes were observed in the activities of antioxidant enzymes, with the “F + T + C + E” group displaying the highest SOD activity (440.7 EU mg-1), and the “F + T” group showing the highest GST activity (542.0 EU mg-1). This research provided comprehensive insights into the biochemical responses of apple fruits to EOC treatments, offering potential strategies for effective fungal management in agriculture.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2024Essential oils in post-harvest disease management: Metabolic impact on Narince (Vitis vinifera L. cv) grapes against Botrytis cinerea
Kaya, Ozkan; Bozkurt, Abdurrahim; Karakus, Sinem; Daler, Selda; Yilmaz, Turhan; Turan, Metin
There is an urgent need for alternative, environmentally friendly post-harvest disease management solutions because of the growing concern over the use of chemical fungicides in agriculture and their detrimental impact on human health and the environment. This study examines the effects of thymol, eugenol, and 1,8-cineole essential oils on the sugar, organic acid, and amino acid profiles of 'Narince' grape berries as well as their effectiveness against gray mold (Botrytis cinerea). Our study has noted that the application of EOs, particularly eugenol, significantly alters the metabolic profile of 'Narince' grapes, enhancing sugar content and modifying the levels of organic acids and amino acids, which suggests a potential for improving grape quality and resistance to Botrytis cinerea. The combined use of eugenol with other EOs and fungicides, as indicated by the treatments OF and OSF, not only displayed a synergistic effect in elevating the concentration of key metabolites but also appeared to be more effective in combating fungal infection compared to individual EO applications. The PCA analysis and subsequent heatmap visualization revealed distinct metabolic clustering among the treatments, with combined EO treatments, especially those including eugenol, markedly influencing the amino acid profile of the berries. Our investigation's results suggest that EOs may offer a viable, eco-friendly alternative to traditional fungicides. Our study also adds to the body of knowledge supporting the advancement of integrated pest management strategies that are efficacious yet environmentally considerate, aligning with the global shift towards more sustainable practices in agriculture and food security.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Zinc Oxide Nanoparticles: An Influential Element in Alleviating Salt Stress in Quinoa (Chenopodium quinoa L. Cv Atlas)
Türkoğlu, Aras; Haliloğlu, Kamil; Ekinci, Melek; Turan, Metin; Yildirim, Ertan; Öztürk, Halil Ibrahim; Stansluos, Atom Atanasio Ladu; Nadaroğlu, Hayrunnisa; Piekutowska, Magdalena; Niedbała, Gniewko
Climate change has intensified abiotic stresses, notably salinity, detrimentally affecting crop yield. To counter these effects, nanomaterials have emerged as a promising tool to mitigate the adverse impacts on plant growth and development. Specifically, zinc oxide nanoparticles (ZnO-NPs) have demonstrated efficacy in facilitating a gradual release of zinc, thus enhancing its bioavailability to plants. With the goal of ensuring sustainable plant production, our aim was to examine how green-synthesized ZnO-NPs influence the seedling growth of quinoa (Chenopodium quinoa L. Cv Atlas) under conditions of salinity stress. To induce salt stress, solutions with three different NaCl concentrations (0, 100, and 200 mM) were prepared. Additionally, Zn and ZnO-NPs were administered at four different concentrations (0, 50, 100, and 200 ppm). In this study, plant height (cm), plant weight (g), plant diameter (mm), chlorophyll content (SPAD), K/Na value, Ca/Na value, antioxidant enzyme activities (SOD: EU g−1 leaf; CAT: EU g−1 leaf; POD: EU g−1 leaf), H2O2 (mmol kg−1), MDA (nmol g−1 DW), proline (µg g−1 FW), and sucrose (g L−1), content parameters were measured. XRD analysis confirmed the crystalline structure of ZnO nanoparticles with identified planes. Salinity stress significantly reduced plant metrics and altered ion ratios, while increasing oxidative stress indicators and osmolytes. Conversely, Zn and ZnO-NPs mitigated these effects, reducing oxidative damage and enhancing enzyme activities. This supports Zn’s role in limiting salinity uptake and improving physiological responses in quinoa seedlings, suggesting a promising strategy for enhancing crop resilience. Overall, this study underscores nanomaterials’ potential in sustainable agriculture and stress management.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Understanding the impact of essential oils on grape metabolism and pathogen resistance: a study with a focus on Botrytis cinerea
Kaya, Ozkan; Karakus, Sinem; Bozkurt, Abdurrahim; Yilmaz, Turhan; Hajizadeh, Hanifeh Seyed; Turan, Metin
Background: The exploration of EOs and their impact on primary metabolites in agricultural products, such as grapes, holds significant importance in the post-harvest preservation and enhancement of berry quality. Therefore, our research aimed to dissect the effects of various EO treatments on the biochemical composition of grapes, specifically examining alterations in sugar, organic acid, and amino acid levels. Results: Our findings highlighted that the OF treatment, a combination of Eugenol and Botrytis cinerea, significantly escalated sugar content, with remarkable increases in sucrose, glucose, and fructose compared to the control group. The SF treatment led to the highest levels of certain organic acids, including tartaric, butyric, and malonic acids, whereas the TO treatment prominently augmented amino acids such as tyrosine and phenylalanine. Utilizing PCA, our study effectively captured the variability within the treatment dataset, revealing distinct clusters of treatments and their correlations with specific metabolites. The PCA biplot underscored the influence of treatments on metabolic profile of grape berries, with treatments like OSF, TO, and TOSF demonstrating close associations with specific amino acids, suggesting positive correlations. Conclusion: Our current results indicate that EOs substantial influence on the metabolic profile of grapes, resulting in enhanced fruit quality and extended shelf life. Variations in sugar, organic acid, and amino acid levels among different EO treatments highlight their potential for improving post-harvest characteristics. To sum up, these findings are valuable for the agricultural and food industries, showcasing the utility of EOs as natural preservatives and enhancers in fruit cultivation and storage. Graphical Abstract: (Figure presented.)
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2023Comparison of contents of sugars, organic acids and free amino acids in raisins obtained from Gök Üzüm (Vitis vinifera L.)
Karakus, Sinem; Ates, Fadime; Keskin, Nurhan; Turan, Metin; Kaya, Ozkan
Raisins are known as an important source of many bioactive compounds such as organic acids, free amino acids, and sugars. In this study, oak ash and potassium carbonate solutions applied before drying were compared for their effects on the Gök Üzüm raisin, more specifically, its contents of organic acids, free amino acids, and sugar profile. The total acidity quantified in the samples of raisin, ranged from 20.30 to 117.08 ng/µL DW for the potassium dipping solution, while values ranged from 17.98 to 164.72 ng/µL DW for the oak ash dipping solution. In samples from either dip solution, serine was predominant, followed by alanine, asparagine, glutamine, aspartate, sarcosine, glutamate, and leucine, whereas gly-cine, arginine, proline, histidine, and valine were found to be the lowest amino acids. In the samples examined, fructose was the most abundant sugar, whereas the lowest sugars in raisins were xylose for oak ash application and galactose for potassium carbonate application. There was wide variation in the sugar profiles for both variants, with values ranging from 3043.48 to 0.71 g/100 g DW. Drying Gök Üzüm grapes after the treatment of oak ash dipping solution promotes a higher content of organic acids, amino acids and sugars as compared to drying after potassium carbonate solution treatments. These results could be used for the development of an attractive pre-drying solution in further studies for the production of both, raisins and perhaps other dried fruits.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023Characterization of volatile compounds of Gök Üzüm raisins produced from grapes pre-treated with different dipping solutions
Karakus, Sinem; Kaya, Ozkan; Hajizadeh, Hanifeh Seyed; Gutiérrez-Gamboa, Gastón; Ates, Fadime; Turan, Metin; Araya-Alman, Miguel
Background: Raisins contain a wide range of secondary metabolites, including volatile compounds that may contribute to the health benefits and preference of consumers. To our knowledge, this is one of the first studies concerning the analysis of volatile compounds in raisin. The goal of this study was to compare volatile composition of Gök Üzüm (Vitis vinifera L.) raisins produced from grapes dried before the application of two pre-treatments solutions: wood ash (WA) and potassium carbonate (K2CO3). Results: Gök Üzüm raisins produced from grapes dipped into the WA solution presented higher contents of most of the studied volatile compounds (including the total contents of C6 compounds, alcohols, benzenoids, esters, aldehydes, terpenes and C13 norisoprenoids) and lower contents of (Z)-2-hexenol and 2-hexenoic acid than the raisins produced from grapes dipped into K2CO solutions. Gök Üzüm raisins were characterized by fruity, floral and grass aromas according to their odor activity values. Drying Gök Üzüm grapes after the treatment of WA solutions promotes a higher aromatic composition compared to K2CO solutions. Conclusion: These findings can greatly assist raisin producers in deciding which dipping solution to use before using a dipping solution to dry the grapes. Graphical Abstract: [Figure not available: see fulltext.]
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023Volatile organic compounds produced by some synthetic essential oils as biological fumigants against Botrytis cinerea on apples
Karakus, Sinem; Atıcı, Okkes; Turan, Metin; Azizi, Sahar; Hajizadeh, Hanifeh Seyed; Kaya, Ozkan
Background: Gray mold, attributed to Botrytis cinerea, poses a substantial threat to food security in fruit-growing regions impacted by global climate change. Addressing this disease requires the utilization of either resilient plant varieties or advanced technological interventions. In this study, the research focused on examining the volatile organic compounds (VOCs) emitted by synthetic essential oils, namely thymol, eugenol, 1,8-cineol, and their combination, as potential biological fumigants against B . cinerea on Golden Delicious apples. Results: In this study, a total of 53 compounds were identified and categorized into six distinct classes, which included (1) terpenes, (2) esters, (3) C6 compounds, (4) alcohols, (5) acids, and (6) aldehydes. The results we obtained revealed significant variations in the volatile compounds present in apples after harvest when treated with different essential oils to combat B. cinerea. Among the VOCs found in the fruits, the most abundant ones were pentanal, nerol, and ethyl octanoate. The essential oil combination of thymol, eugenol, and 1,8-cineol (Thy + Eug + Fun) had the most significant impact on the volatile compound content in the fruits. Conversely, both B. cinerea and the essential oils were observed to increase the volatile organic compound content in the fruits after harvest. Conclusion: The findings from this study underscore the significance of essential oils as effective biological fumigants for countering Botrytis cinerea on apples. Furthermore, the study suggests that these essential oils have the potential to influence the composition of volatile organic compounds in postharvest apples. This research offers valuable insights into the intricate interplay between volatile organic compounds and essential oils in apples, emphasizing the critical role of essential oils in preserving fruit quality during the post-harvest period. Graphical abstract: [Figure not available: see fulltext.].
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2022Antioxidant Activity, Phenolic Composition, and Hormone Content of Wild Edible Vegetables
Ozturk, Halil Ibrahim; Nas, Hazel; Ekinci, Melek; Turan, Metin; Ercisli, Sezai; Narmanlioglu, Haluk Kemal; Yildirim, Ertan; Assouguem, Amine; Almeer, Rafa; Sayed, Amany A.
Wild edible plants have been used since antiquity as folk medicine and as preservatives in foods. This study aimed to determine the antioxidant activities, phenolic compounds, and hormone contents of 12 species of edible wild plants belonging to 9 families, which are consumed as vegetables by the local people at Ergan Mountain in Erzincan in Turkey. Polygonum cognatum and Malva neglecta were determined to have more antioxidant enzyme activity, more phenolic compounds, and higher hormone content than the other species. The highest catalase (CAT), peroxidase (POD), glutathione reductase (GR), glutathione-s-transferase (GST) values for P. cognatum were determined as 45.12, 94.83, 36.76, and 1218.35 EU g−1, respectively. The highest superoxide dismutase (SOD) and ascorbate peroxidase (AxPOD) content for M. neglecta were determined as 97.53 EU g−1 and 81.93 EU g−1, respectively. P. cognatum is the species in which the highest levels of the hormones indolacetic acid (IAA), gibberellic acid (GA), salicylic acid (SA), cytokinin, zeatin and jasmonic acid were detected. The highest levels of caftaric acid (CA), catechin (CAE), ferulic acid (FA), malvidin-3-o-glucoside (MG), myricetin (MYR), rutin (RT), trans-coumaric acid (TPCA), tyrosol (TY), and vanilic acid (VA) compounds were found in M. neglecta. It was determined that Falcaria vulgaris species had the highest levels of ferulic acid (FA) and quercetin (QUE) phenolics. The results show that edible wild vegetables consumed and studied by the people of the region are an important source of natural antioxidants. The possibilities of using these wild plants as functional foods should be investigated.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2022Changes in Biogenic Amines of Two Table Grapes (cv. Bronx Seedless and Italia) during Berry Development and Ripening
Incesu, Melek; Karakus, Sinem; Seyed Hajizadeh, Hanifeh; Ates, Fadime; Turan, Metin; Skalicky, Milan; Kaya, Ozkan
Bronx Seedless and Italia (Vitis vinifera L.) are a variety preferred by consumers owing to their exciting flavour and widely cultivated in Aegean Region in Turkey. The aim was to identify the biogenic amines of these table grapes during berry ripeness. The biogenic amines were analyzed by HPLC in six different berry phenological stages. Italia grapes presented lower biogenic amine content than Bronx Seedless table grapes. The concentration of most of the biogenic amines analyzed linearly raised from the beginning of berry touch to when berries ripen for harvest stages. The most common biogenic amines in grape varieties were putrescine, followed by histamine, agmatine, and tyramine. There was also a positive correlation between all biogenic amines of the two grape varieties. The weakest correlation was found between spermine and cadaverine, whereas the strongest correlation was found among dopamine, trimethylamine, norepinephrine, tyramine, and histamine amines. The present study is the first report of a synthesis study regarding the effect of B.A.s on quality characteristics throughout berry ripeness in grape varieties containing foxy and muscat tastes. The concentration and composition of biogenic amines identified for both varieties might provide helpful information regarding human health and the vintage.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2016A multicenter evaluation of blood culture practices, contamination rates, and the distribution of causative bacteria
Altindis, Mustafa; Koroglu, Mehmet; Demiray, Tayfur; Dal, Tuba; Ozdemir, Mehmet; Sengil, Ahmet Zeki; Atasoy, Ali Riza; Turan, Metin; Cicek, Aysegul Copur; Ece, Gulfem
Background: The prognostic value of blood culture testing in the diagnosis of bacteremia is limited by contamination. Objectives: In this multicenter study, the aim was to evaluate the contamination rates of blood cultures as well as the parameters that affect the culture results. Materials and Methods: Sample collection practices and culture data obtained from 16 university/research hospitals were retrospectively evaluated. A total of 214,340 blood samples from 43,254 patients admitted to the centers in 2013 were included in this study. The blood culture results were evaluated based on the three phases of laboratory testing: the pre-analytic, the analytic, and the post-analytic phase. Results: Blood samples were obtained from the patients through either the peripheral venous route (64%) or an intravascular catheter (36%). Povidone-iodine (60%) or alcohol (40%) was applied to disinfect the skin. Of the 16 centers, 62.5% have no dedicated phlebotomy team, 68.7% employed a blood culture system, 86.7% conducted additional studies with pediatric bottles, and 43.7% with anaerobic bottles. One center maintained a blood culture quality control study. The average growth rate in the bottles of blood cultures during the defined period (1259 -26,400/year) was 32.3%. Of the growing microorganisms, 67% were causative agents, while 33% were contaminants. The contamination rates of the centers ranged from 1% to 17%. The average growth time for the causative bacteria was 21.4 hours, while it was 36.3 hours for the contaminant bacteria. The most commonly isolated pathogens were Escherichia coli (22.45%) and coagulase-negative staphylococci (CoNS) (20.11%). Further, the most frequently identified contaminant bacteria were CoNS (44.04%). Conclusions: The high contamination rates were remarkable in this study. We suggest that the hospitals’ staff should be better trained in blood sample collection and processing. Sterile glove usage, alcohol usage for disinfection, the presence of a phlebotomy team, and quality control studies may all contribute to decreasing the contamination rates. Health policy makers should therefore provide the necessary financial support to obtain the required materials and equipment.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2014Protective role of humic acids against dicamba-induced genotoxicity and DNA methylation in Phaseolus vulgaris L.
Yildirim, Nalan; Agar, Guleray; Taspinar, Mahmut Sinan; Turan, Metin; Aydin, Murat; Arslan, Esra
Dicamba is one of herbicides used widely in agriculture today. The wide use of dicamba in agriculture represents a potential danger to the ecosystems and the environment. Thus, the present study is aimed to investigate the DNA damage levels and the DNA methylation changes in Phaseolus vulgaris subjected to whether dicamba and humic acids (HAs) have any protective effect on these changes. Randomly Amplified Polymorphic DNAs (RAPDs) and Coupled Restriction Enzyme Digestion-Random Amplification (CRED-RAs) were used to determinate the DNA damage levels and the changes in the pattern of DNA methylation. The results showed that dicamba (0.2, 0.4 and 0.6 ppm) caused RAPDs profile changes (DNA damage) as increasing, genomic template stability (GTS) as decreasing and DNA hypomethylation. However, these effects of dicamba seen at higher levels decreased after treatment with five different concentrations (2, 4, 6, 8 and10%) of HAs. The results of this experiment have clearly shown that HAs could be used effectively to protect bean seedlings from the destructive effects of dicamba.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2012CHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITY OF FOXTAIL LILY (Eremurus spectabilis)
Tosun, Murat; Ercisli, Sezai; Ozer, Hakan; Turan, Metin; Polat, Taskin; Ozturk, Erdogan; Padem, Huseyin; Kilicgun, Hasan
Eremurus spectabilis is used as a vegetable in Turkey, especially in Eastern Anatolia region. In this study, eight E. spectabilis from different growing areas have been analyzed for its nutrition value and antioxidant properties. The results showed that there were significant differences among samples in terms of all above parameters. The mean values of the parameters investigated were 86.62-91.35% for water content, 4.78-5.15 for pH; 0.42-0.70% for acidity, and 0.61-1.11% for ash content. The antioxidant activity tests evaluated by using 2-diphenyl-1-picryhydrazyl (DPPH) free radical scavenging and beta-carotene/linoleic acid assays indicated that the extracts of E. spectabilis samples had high antioxidant capacity. In the DPPH and beta-carotene/linoleic acid systems, average values were 73.69 mu g extract. ml(-1) and 94.56%, respectively. The average amount of total phenolics in samples was 223 mg GAE . 100 g(-1) FW. Protein, K, Ca, Mg, Fe and Cu contents of E. spectabilis species were found higher than in some other commonly used vegetables. The results suggest that E. spectabilis could be a valuable source of antioxidants, phenolics and minerals.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2012Chemical composition and antioxidant activity of foxtail lily (Eremurus spectabilis); [Skład chemiczny i działanie przeciwutleniające pustynnika okazałego (eremurus spectabilis)]
Tosun, Murat; Ercisli, Sezai; Ozer, Hakan; Turan, Metin; Polat, Taskin; Ozturk, Erdogan; Padem, Huseyin; Kilicgun, Hasan
Eremurus spectabilis is used as a vegetable in Turkey, especially in Eastern Anatolia region. In this study, eight E. spectabilis from different growing areas have been analyzed for its nutrition value and antioxidant properties. The results showed that there were significant differences among samples in terms of all above parameters. The mean values of the parameters investigated were 86.62-91.35% for water content, 4.78-5.15 for pH; 0.42-0.70% for acidity, and 0.61-1.11% for ash content. The antioxidant activity tests evaluated by using 2-diphenyl-1-picryhydrazyl (DPPH) free radical scavenging and β-carotene/linoleic acid assays indicated that the extracts of E. spectabilis samples had high antioxidant capacity. In the DPPH and β-carotene/linoleic acid systems, average values were 73.69 μg extract · ml-1 and 94.56%, respectively. The average amount of total phenolics in samples was 223 mg GAE · 100 g-1 FW. Protein, K, Ca, Mg, Fe and Cu contents of E. spectabilis species were found higher than in some other commonly used vegetables. The results suggest that E. spectabilis could be a valuable source of antioxidants, phenolics and minerals.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2010Katı, sıvı ve kombine sığır gübresi uygulamalarının adi fiğ + arpa karışımlarında verim, kalite ve mineral içerikler üzerine etkileri
Serin, Yunus; Turan, Metin; Yolcu, Halil; Daşcı, Mahmut; Güneş, Adem
A field experiment was conducted to evaluate the effects of solid, liquid and the combination of solid and liquid cattle manure applications on the yield, quality and mineral content of common vetch (Vicia sativa L.) + barley (Hordeum vulgare L.) intercropping mixture in 2006-2007 at Gümü&#254;hane, Turkey. The treatments were control (no manure), two liquid ($L_1$ = 10 000 L $ha^{-1}$ liquid cattle manure and $L_2$ = 20 000 L $ha^{-1}$ liquid cattle manure), two solid ($S_1$ = 20 ton $ha^{-1}$ solid cattle manure, S2= 40 ton $ha^{-1}$ solid cattle manure) and four different combinations of liquid and solid cattle manure applications ($S_1L_1$ = 20 ton $ha^{-1}$ solid cattle manure + 10 000 L $ha^{-1}$ liquid cattle manure, $S_1L_2$ = 20 ton $ha^{-1}$ solid cattle manure + 20 000 L $ha^{-1}$ liquid cattle manure, $S_2L_1$ = 40 ton $ha^{-1}$ solid cattle manure + 10 000 L $ha^{-1}$ liquid cattle manure, and $S_2L_2$ = 40 ton $ha^{-1}$ solid cattle manure + 20 000 L $ha^{-1}$ liquid cattle manure). Total forage dry matter yield (TFDMY), crude protein content (CP), crude protein yield (CPY), acid detergent fiber (ADF), neutral detergent fiber (NDF), dry matter intake (DMI), dry digestible matter (DDM), relative feed value (RFV) and mineral contents (Mg, Ca, K, P, Na, Mn, Zn, Fe and Cu) were determined to evaluate the effects of different cattle manure applications on intercropping mixtures. Solid, liquid and the combined cattle manure treatments had no effect on the TFDMY, CPY, ADF, NDF, DMI, DDM, RFV, Mg, Ca, K, Mn and Zn of the intercropping mixture except CP (p<0.01), P (p<0.01), Na (p<0.05), Fe (p<0.05) and Cu (p<0.05) in the first year. The treatments had significant effects on TFDMY (p<0.01), CP (p<0.01), ADF (p<0.05), NDF (p<0.01), DMI (p<0.01), DDM (p<0.05), RFV (p<0.01) and Zn (p<0.01) values of the intercropping mixture except CPY, Mg, Ca, K, P, Na, Mn, Fe and Cu in the second year. The results of the study revealed that the common vetch and barley intercropping mixture can be fertilized with 40 ton $ha^{-1}$ solid cattle manure to improve the yield and quality. But if mineral content of forage is more important than yield and quality for the farmer, then it may be fertilized with 20 ton $ha^{-1}$ solid cattle manure.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2008Mineral content of some Lucerne cultivars for livestock
Yolcu, Halil; Dasci, Mahmut; Turan, Metin
The purpose of this study was to assess the mineral contents of twelve lucerne cultivars on the highlands for livestock. Lucerne cultivars (Bilensoy, alfa-484, Resis, alfa-1312, Savas, Kayseri, alfa-1313, Planet, Seker, Daisy, Bilensoy-80, CW-3567) were collected from cultivated fields. In general, Ca, Fe and Mn contents of the lucerne cultivars were the highest in Bilensoy cultivar, N in Planet cultivar, K in Savas cultivar, P in Daisy cultivar, Mg in alfa-1313 cultivar and Zn and Cu in Bilensoy-80 cultivar. Bilensoy, Daisy and Seker cultivars especially had very high mineral contents. The determination of mineral contents of Lucerne cultivars has been a phase in the evaluation of impacts to animal feeding. Lucerne cultivars had a very high nutritional potential and their N, P, K, Ca, Mg, Na, Fe and Zn values were higher than those required for animal nutrition. Because of high digestion, taste, cheapness of Lucerne plant and provision of macro and micro mineral, it has very important raw material nutrition for animal nourishment.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2008The effects of various fertilization and seeding patterns on trace minerals content of lucerne and smooth bromegrass mixture in an intercropping system for livestock
Yolcu, Halil; Turan, Metin
The effects of various fertilization and seeding patterns on trace mineral contents of lucerne-smooth bromegrass mixture were evaluated for animal feeding. The lucerne and smooth bromegrass mixtures were established with different seeding pattern (mixed, alternative and cross-seeding), N fertilizer rates (0, 60 and 120 kg N ha-1) and P fertilizer rates (0, 40, 80 and 120 P2O5 ha-1). Different seeding patterns, N and P fertilization affected the trace mineral contents of the lucerne-smooth bromegrass mixture under the intercropping system. Mn, Cu and Zn content of the mixture varied significantly depending on seeding patterns. However, the seeding patterns had no significant effect on the Fe contents of the mixture. N application decreased the content of Fe and increased the content of Cu, but had no effect on the Mn and Zn content; whereas, P application affected the Mn and Cu contents but had no effect on the contents of Fe and Zn.
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