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An overview of ELISA: a review and update on best laboratory practices for quantifying peptides and proteins in biological fluids

Review ArticleAçık Erişim

Özet

The enzyme-linked immunosorbent assay (ELISA) detects antigen-antibody interactions by using enzyme-labelled conjugates and enzyme substrates that generate colour changes. This review aims to provide an overview of ELISA, its various types, and its applications in detecting metabolites in biological fluids. The article discusses the history of the assay, its underlying principles and procedures, common ELISA protocols, and the most accurate and reliable techniques for measuring peptide molecules in biological fluids. Additionally, we emphasize best laboratory practices to achieve consistent, high-quality results and outline the essential materials for setting up an ELISA laboratory, drawing from our over 30 years of experience in the field. © The Author(s) 2025.

Anahtar Kelimeler

Biological fluids, ELISA, antibody, antigenenzyme-linked immunosorbent assayBody FluidsHumansPeptidesProteinsantigenpeptides and proteinspeptideproteinantigen antibody reactionbody fluidcolorconjugatedrug therapyELISA kitenzyme linked immunosorbent assayenzyme substratehumanmetaboliteprotocolreviewchemistryimmunologymetabolismprocedures
RDL Kalıcı Bağlantı (PID)
https://rdlopen.rawdatalibrary.net/r/ebyu/11323Resmi atıflarda kullanılan kalıcı adres
DOI
https://doi.org/10.1177/03000605251315913Yayıncı resmi DOI adresi
Tam Kayıt
AlanDeğerDil
dc.contributor.authorAydin, Suleyman
dc.contributor.authorEmre, Elif
dc.contributor.authorUgur, Kader
dc.contributor.authorAydin, Mustafa Ata
dc.contributor.authorSahin, İbrahim
dc.contributor.authorCinar, Vedat
dc.contributor.authorAkbulut, Taner
dc.date.accessioned2026-10-18T20:26:23.524155089Z
dc.date.available2026-10-18T20:26:23.524155089Z
dc.date.issued2025
dc.description.abstractThe enzyme-linked immunosorbent assay (ELISA) detects antigen-antibody interactions by using enzyme-labelled conjugates and enzyme substrates that generate colour changes. This review aims to provide an overview of ELISA, its various types, and its applications in detecting metabolites in biological fluids. The article discusses the history of the assay, its underlying principles and procedures, common ELISA protocols, and the most accurate and reliable techniques for measuring peptide molecules in biological fluids. Additionally, we emphasize best laboratory practices to achieve consistent, high-quality results and outline the essential materials for setting up an ELISA laboratory, drawing from our over 30 years of experience in the field. © The Author(s) 2025.
dc.identifier.doi10.1177/03000605251315913
dc.identifier.eissn1473-2300
dc.identifier.issn0300-0605
dc.identifier.issue2
dc.identifier.pmcidPMC11808753
dc.identifier.pmid39922798
dc.identifier.scopus2-s2.0-85217404036
dc.identifier.startpage3000605251315913
dc.identifier.urihttps://rdlopen.rawdatalibrary.net/r/ebyu/11323
dc.identifier.volume53
dc.identifier.wosWOS:001415869000001
dc.language.isoen
dc.publisherSAGE Publications Ltd
dc.relation.ispartofJournal of International Medical Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBiological fluids, ELISA, antibody, antigen
dc.subjectenzyme-linked immunosorbent assay
dc.subjectBody Fluids
dc.subjectHumans
dc.subjectPeptides
dc.subjectProteins
dc.subjectantigen
dc.subjectpeptides and proteins
dc.subjectpeptide
dc.subjectprotein
dc.subjectantigen antibody reaction
dc.subjectbody fluid
dc.subjectcolor
dc.subjectconjugate
dc.subjectdrug therapy
dc.subjectELISA kit
dc.subjectenzyme linked immunosorbent assay
dc.subjectenzyme substrate
dc.subjecthuman
dc.subjectmetabolite
dc.subjectprotocol
dc.subjectreview
dc.subjectchemistry
dc.subjectimmunology
dc.subjectmetabolism
dc.subjectprocedures
dc.titleAn overview of ELISA: a review and update on best laboratory practices for quantifying peptides and proteins in biological fluids
dc.typeReview Article
dspace.entity.typePublication
rdl.citation.scopus178
rdl.citation.wos172
rdl.departmentErzincan Binali Yildirim University, School of Medicine, Department of Medical Biology, Erzincan, Turkey.
rdl.oa.statusgold
rdl.source.indexSCOPUS
rdl.source.indexWOS
rdl.source.indexPUBMED
rdl.wos.indexScience Citation Index Expanded (SCI-EXPANDED)