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93 →- ArticleYayıncıda açık erişim2027From extraction to ocular delivery: An integrated approach using apigenin and apigenin 7-O-glucoside nanosuspensions to treat corneal neovascularization
Aytekin, Eren; Gözcü, Sefa; Polat, Heybet Kerem; Akşit, Hüseyin; Karakuyu, Nasıf Fatih; Kurt, Nihat; Sofu, Gülşah Usta; Akkaya, Züleyha Yalnız; Tebrizi, Behzat; Çalış, Sema
Corneal neovascularization (CV) is a vision-threatening condition characterized by the pathological ingrowth of blood vessels. While corticosteroids and anti-VEGF agents are standard treatments, their prolonged use is associated with significant ocular side effects. This study aimed to develop and optimize nanosuspensions of Apigenin (APG) and Apigenin-7-O-glucoside (APG 7) to enhance their ocular bioavailability and evaluate their efficacy in the treatment of CV. APG and APG 7 were isolated from Petroselinum crispum . Nanosuspensions were formulated using an anti-solvent precipitation method, optimized via a 23 factorial design considering PVA concentration, Span 80 concentration, and homogenization speed. The physical properties, including particle size, PDI, and zeta potential, were characterized. In vivo efficacy was assessed over a 7-day treatment period in an alkali-burn induced rat CV model through clinical scoring, histopathology, and immunohistochemical analysis of VEGFA and IL-6 expression. The optimized APG nanosuspensions exhibited a mean particle size of ∼166.9 nm with high stability. In vivo studies demonstrated that 0.05% APG nanosuspension significantly inhibited corneal angiogenesis, reduced stromal edema, and downregulated VEGFA and IL-6 expression compared to the untreated injury group (p < 0.05). Conversely, APG 7 formulations failed to show significant therapeutic efficacy, likely due to limited corneal permeability attributed to the glycoside moiety. The 0.05% APG nanosuspension presents a promising, non-invasive therapeutic alternative for CV management, offering effective anti-angiogenic activity with improved ocular delivery characteristics.
RDL Kalıcı Kaydıİncele - Conference ObjectYalnız künye2026Investigation of the Anticancer Efficacy of the Combination 6-Gingerol and Apigenin in Cervical Cancer Cells
Çetinkaya, B. Demir; Gözcü, S.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2026Chemical Composition and Antibacterial Activity of the Essential Oil from Cupressus arizonica Greene Cones Against Clinical and Foodborne
Gözcü, Sefa; Akşit, Zeynep; Dursun, Ahmet
Objective: The purpose of this study was to characterize the chemical composition of the essential oil (EO) derived from the cones of Cupressus arizonica Greene collected from Erzincan and to evaluate its antibacterial potential against a range of pathogenic microorganisms, including strains not previously investigated for this species. Methods: The essential oil was obtained from fresh cones via hydrodistillation for 4 hours. Chemical profiling was performed using Gas Chromatography-Mass Spectrometry (GC-MS). The antibacterial activity was assessed against six pathogenic bacteria using the disc diffusion method, with imipenem serving as a positive control. Results: GC-MS analysis identified 35 compounds, representing 93.83% of the total oil. The major components were α -pinene (53.02%), 3-carene (13.31%), α -myrcene (4.54%), sabinene (4.24%), and limonene (3.47%). The oil exhibited selective antibacterial activity, with inhibition zones recorded for Salmonella enterica (10.0 ± 0.3 mm), Clostridium perfringens (8.1 ± 0.5 mm), and Bacillus cereus (8.0 ± 0.6 mm). However, no inhibitory effect was observed against Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. Conclusion: This study identifies a distinct α-pinene/3-carene chemotype of C. arizonica influenced by the regional flora of Erzincan. Notably, this research represents the first report of the inhibitory efficacy of C. arizonica cone essential oil against the anaerobic pathogen Clostridium perfringens. The findings suggest that this essential oil could be a promising natural candidate for targeted applications against certain foodborne and anaerobic pathogens.
RDL Kalıcı Kaydıİncele - ArticleYayıncıda açık erişim2026Comparative evaluation of the cytotoxic and antioxidant effects of apricot (Prunus armeniaca L.) fruit and traditionally prepared apricot jam: Differential impacts on healthy and cancer cell lines
Güven, Zühal Bayrakçeken; Gülmüş, Cennet
Cancer is a complex, multifactorial disease in which the toxic side effects of drugs hinder patients' quality of life and adherence to treatment. During this period, people try to cope with the negative effects of treatment and disease by consuming natural foods. This study compared the antiproliferative and antioxidant effects of apricot (Prunus armeniaca L.) fruit and traditionally prepared jam. Cytotoxic effects were investigated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method in L929, A549, CaCo-2, Hep3B, MCF-7 and PC-3 cell lines. Antioxidant capacity was determined by DPPH, CUPRAC, and TEAC methods. While jam exhibited cytotoxic effects in healthy cells at concentrations exceeding 400 µg/mL, the fruit aqueous extract did not reduce cell viability. In cancer cells, the fruit aqueous extract showed a concentration-dependent cytotoxic effect, with 64.60% and 58.8% cytotoxicity observed against the Hep-3B cell line at 800 and 1600 µg/mL, respectively. Notably, jam was found to promote cancer cell proliferation at higher concentrations, a critical observation that contrasts sharply with the fruit extract's antiproliferative effects. In all experiments, the aqueous extract showed a higher antioxidant effect than the jam. The results indicate that the antiproliferative and antioxidant effects of jam compared to fruit were reduced due to added sugar and high temperature.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2026Comparative phytochemical profiling, enzyme inhibitory activities, and in silico pharmacokinetic insights of essential oils and extracts from Origanum leptocladum Boiss. and Origanum vulgare subsp. hirtum (link) A.Terracc
Günes, Ferhat; Çeçen, Omer; Özer, Elif Beyza; Yuca, Hafize; Aydin, Bilge; Demirci, Betul; Karakaya, Songul
This study comparatively evaluated the phytochemical and biological profiles of Origanum leptocladum and O. vulgare subsp. hirtum (Lamiaceae). Aqueous extracts yielded more material than methanolic ones, with O. leptocladum showing superior extractability. GC-MS analysis revealed distinct chemotypes: O. leptocladum was rich in cis-sabinene hydrate and p-cymene, whereas O. vulgare was dominated by carvacrol. Methanol extracts of O. leptocladum exhibited the highest alpha-glucosidase inhibition (90.10%) and antioxidant capacity, correlating with elevated phenolic content. Molecular docking indicated strong binding affinities of carvacrol, cis-sabinene hydrate, and p-cymene to alpha-glucosidase, alpha-amylase, AChE, and BChE, implying multitarget inhibitory potential. ADME predictions confirmed favourable pharmacokinetic properties and BBB permeability, especially for carvacrol and cis-sabinene hydrate. This first report on alpha-glucosidase and alpha-amylase inhibition by O. leptocladum underscores its promising antidiabetic and neuroprotective potential, supporting its value for pharmaceutical and nutraceutical development.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2026From aquatic plant to therapeutic prospect: LC-MS/MS-guided phytochemical profiling and in vitro antidiabetic, anticholinesterase and molecular insights into Myriophyllum spicatum L.
Civas, Ayse; Günes, Ferhat; Özer, Elif Beyza; Imad Abdulqader, Amenah; Akyüz, Beyzanur; Yuca, Hafize; Aydin, Bilge; Karakaya, Songul
Myriophyllum spicatum L. methanolic extract was examined through integrated biochemical, phytochemical, computational analyses. The extract exhibited strong alpha-glucosidase inhibitory activity with an IC50 value of 198.54 & micro;g/mL, demonstrating markedly higher potency than reference inhibitor acarbose (IC50= 4738.12 & micro;g/mL), while no inhibitory activity was detected against alpha-amylase under tested conditions. In anticholinesterase assays, extract showed acetylcholinesterase inhibition with an IC50 value of 9.70 & micro;g/mL, whereas standard inhibitor donepezil exhibited a significantly stronger activity (IC50= 0.30 & micro;g/mL). No inhibitory effect was observed against butyrylcholinesterase for the extract, while donepezil displayed potent inhibition (IC50= 15.40 & micro;g/mL). Extract showed strong ABTS center dot+ scavenging activity (85.7% at 40 & micro;g/mL) but weak DPPH center dot activity (25.6% at 25 & micro;g/mL), suggesting higher affinity for hydrophilic radicals. High total phenolic, flavonoid and tannin contents correlated with antioxidant potential. LC-MS/MS revealed thirteen phenolics, mainly vanillic acid, gallic acid and cyanidin-3-O-glucoside. Docking studies supported selective alpha-glucosidase binding of vanillic acid.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2026Development and characterization of olopatadine and verbascoside-loaded thermoresponsive in situ gels for ocular drug delivery: a factorial design approach for allergic conjunctivitis
Polat, Heybet Kerem; Çopur, Tuğba; Pezik, Esra; Turanlı, Yasin; Karakuyu, Nasıf Fatih; Köngül, Esra; Ünal, Sedat; Gözcü, Sefa; Sarman, Emine
Objective: Allergic conjunctivitis is one of the most prevalent conditions affecting the eyes, and various medications are used in its treatment. In this study, olopatadine and verbascoside were used in combination. Methods: A factorial design was employed to evaluate the effects of Poloxamer 407 and sodium alginate. The impact of polymer concentration on parameters such as viscosity, gelation time, mucoadhesion, pH, and gelation temperature–considered indicators of ideal formulations–was assessed. Results: The optimal formulation, determined through the factorial design, included 0.5% sodium alginate and 20% poloxamer 407. The obtained values were viscosity at 25 °C = 7451.8 cP, viscosity at 35 °C = 11367.3 cP, pH = 7.12, gelation temperature = 30 °C, gelation time = 52 s, and mucoadhesion = 0.301 N. Cytotoxicity tests on ARPE-19 and L929 cells, along with irritation studies, indicated acceptable safety profiles. In vitro release tests demonstrated an initial burst release of verbascoside and olopatadine during the first 2 h, followed by a controlled release over the subsequent 8 h. Mathematical analysis of the release kinetics revealed a good fit with the Weibull model. No adverse effects were observed in rats during Draize’s in vivo test. Conclusions: Consequently, the drug-loaded in situ gel formulation may be a promising option for treating allergic conjunctivitis.
RDL Kalıcı Kaydıİncele - ArticleYayıncıda açık erişim2026Therapeutic effects of Malus floribunda extract on letrozole-induced insulin resistance in rats
Ozdemir Sarikaya, Bengul; Gul, Huseyin Fatih; Gozcu, Sefa; Yayla, Muhammed
The present study aimed to investigate the therapeutic effects of Malus floribunda fruit ethanol extract on metabolic parameters and insulin signalling in a letrozole-induced PCOS-associated insulin resistance model. 60 female Sprague-Dawley rats were randomly allocated into five groups: Healthy Control, IR, IR + Metformin (300 mg/kg), IR + MF250, and IR + MF500. Treatments were administered orally for 15 days. Fasting insulin, HOMA-IR, OGTT, lipid profile, and reproductive hormones were assessed. Histological and IHC analyses of InR, IRS-1, and IRS-2 were performed on muscle and adipose tissues. The IR group exhibited significantly elevated insulin (p<0.001), HOMA-IR (p<0.001), testosterone, triglycerides, LDL, and total cholesterol levels, with reduced HDL and oestrogen levels compared to controls. Treatment with M. floribunda, particularly at 500 mg/kg, significantly improved insulin levels (p<0.001), HOMA-IR (p<0.001), lipid profile, and hormonal parameters. IHC analysis revealed suppressed InR, IRS-1, and IRS-2 expression in muscle and adipose tissues of the IR group, which was restored following M. floribunda treatment. M. floribunda extract administration, particularly at 500 mg/kg, significantly improved glucose homeostasis, lipid metabolism, hormonal balance, and insulin signalling pathway components (InR, IRS-1, IRS-2) in a letrozole-induced PCOS-associated insulin resistance model. These findings suggest that M. floribunda may serve as a promising alternative therapeutic agent for insulin resistance management.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2026Chemical Composition and Antibacterial Activity of Rosa villosa subsp. mollis R. Keller & Gams., Rosa hemisphaerica J. Herrm. and Natural Hybrid Rosa ozcelikii Korkmaz & Kandemir Essential Oil
Gözcü, Sefa; Akşit, Zeynep; Akşit, Hüseyin; Şimşek, Samed; Korkmaz, Mustafa
This study explores the essential oils (EOs) of Rosa hemisphaerica (RH), Rosa villosa subsp. mollis (RV) and the newly identified hybrid Rosa ozcelikii (RO). The major components of the EOs were found to be nonadecane (22.45%), viridiflorol (19.37%) and heneicosane (11.72%) in RH; α-muurolene (14.72%), σ-cadinene (17.88%) and farnesol (9.94%) in RV; and heptacosane (27.63%), heneicosane (12.42%) and α-citronellol (11.90%) in RO. Antibacterial activities were evaluated using the disc diffusion method. The EOs demonstrated moderate antibacterial effects: RV and RH exhibited inhibition zones of 8.0 ± 0.4 mm against Pseudomonas aeruginosa, while RO showed stronger activity with inhibition zones of 11.0 ± 0.5 mm against Staphylococcus aureus, 12.0 ± 0.4 mm against Clostridium perfringens and 9.0 ± 0.5 mm against Bacillus cereus. These findings highlight the chemical diversity and potential therapeutic applications of these Rosa species, emphasising their relevance in the search for natural antimicrobial agents.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2026Therapeutic Effects of Cichorium intybus L. Extract in an Experimentally Induced Insulin Resistance Model in Rats
Ermis, Uğur; Gül, Hüseyin Fatih; Gözcü, Sefa; Özdemir, Bengül; Hamdemir, Özgecan; Binnetoğlu, Damla
Introduction: Insulin resistance (IR) is a central metabolic feature of polycystic ovary syndrome (PCOS). Althou gh metformin improves insulin sensitivity, its adverse effects limit long-term use, prompting interest in herbal alternatives. The present study aimed to investigate the effects of Cichorium intybus L. ethanolic extract on PCOS-associated IR in a letrozole-induced rat model, compared with metformin. Materials and Methods: A total of twenty-four female Sprague–Dawley rats were allocated into four groups: healthy control (H), PCOS-associated insulin resistance (IR), metformin-treated (MET), and Cichorium intybus ethanolic extract–treated (C.I). PCOS was induced by oral letrozole administration (1 mg/kg/day) for 21 days. Following confirmation of IR by estrous cycle disruption and oral gl ucose tolerance test (OGTT), treatments were administered orally for 15 da ys. Metabolic parameters, serum insulin, glucose, lipid profile, sex hormones, and HOMA-IR index were evaluated. Skeletal muscle tissues were examined histopathologically and by immunohistochemical analysis of insulin receptor (INSR), insulin receptor subsrate 1 (IRS1), and insulin receptor subsrate 2 (IRS2) expression. Results: PCOS-associated IR rats showed impaired glucose tolerance, hyperinsulinemia, increased HOMA-IR, dyslipidemia, and hormonal imbalance. C. intybus ethanolic extract treatment significantly improved glucose tolerance, insulin levels, HOMA-IR, and lipid profile, with effects comparable to metformin. While metformin markedly restored INSR, IRS1, and IRS2 expression in skeletal muscle, C. intybus produced only moderate improvements. Conclusion: C. intybus ethanolic extract improved metabolic, hormonal, and biochemical paramete rs in PCOS-associated IR. However, its effects on skeletal muscle insulin signaling markers were less pronounced than metformin, suggesting a complementary therapeu tic role mainly through systemic metabolic regulation.
RDL Kalıcı Kaydıİncele
Tam Kayıt
| Alan | Değer | Dil |
|---|---|---|
| dc.date.accessioned | 2026-10-20T15:26:12.758544080Z | |
| dc.date.available | 2026-10-20T15:26:12.758544080Z | |
| dc.identifier.uri | https://rdlopen.rawdatalibrary.net/r/ebyu/22900 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.title | Farmakognozi Anabilim Dalı | tr |
| dc.title | Department of Pharmacognosy | en |
| dspace.entity.type | OrgUnit | |
| organization.identifier.code | ecz-farmakognozi | |
| organization.unitType | department | |
| rdl.unit.alias | pharmacogn |