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- ArticleYalnız künye2016Effects of nimesulide on oxidative mucosal injury induced by cisplatin in rat duodenum and jejunum
Ozcicek, Fatih; Cankaya, Murat; Cimen, Ferda K.; Kafa, Ayse H.T.; Nalkiran, Hatice S.; Suleyman, Bahadir; Altuner, Durdu; Cetin, Nihal
In this study, the impact of nimesulide on oxidative stress induced by cisplatin in rat duodenum and jejunum tissue was assessed by evaluating the biochemical and histopathological changes. The rats in the study were assigned to three groups: the control group and cisplatin (Cis) group were given distilled water, and the nimesulide + cisplatin (Nim + Cis) group was given nimesulide (50 mg/kg) orally for seven days. The Cis and Nim + Cis groups were injected with a single dose of intraperitoneal cisplatin (6 mg/kg) on the first day. After the rats were euthanized, the duodenum and jejunum of each rat was removed for the assessment of biochemical markers and histopathological evaluation. Cisplatin administration in the Cis group resulted in increased levels of malondialdehyde and nitric oxide and decreased levels of total glutathione, glutathione reductase, and catalase compared to the healthy and Nim + Cis groups. Nimesulide significantly inhibited the increase of oxidants (p < 0.001) and the decrease of antioxidants caused by cisplatin (p < 0.001). Inflammation, damage to the villus epithelium, hemorrhage, and capillary proliferation were determined histopathologically in the Cis group. The results of this study indicate that oxidative stress caused by cisplatin may be preventable by co-administered nimesulide.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2016The effect of metamizole on ischemia/reperfusion injury in the rat ovary: An analysis of biochemistry, molecular gene expression, and histopathology
Kumbasar, Serkan; Salman, Suleyman; Al, Ragip; Ozturk, Cengiz; Yarali, Oguzhan; Alp, Hamit; Altuner, Durdu; Suleyman, Bahadir
Objectives: In this study, we investigated the effect of metamizole on ischemia/reperfusion (I/R) injury an analysis of biochemistry, molecular gene expression, and histopathology in the rat ovary of female albino Wistar rats. Materials and Methods: Animals were divided into four groups; control group with induced ischemia-reperfusion (IRC), ischemia-reperfusion +100 mg/kg metamizole sodium (MS) (IRM-100), ischemia-reperfusion +200 mg/kg MS (IRM-200), and healthy group applied sham operation (SG). Results: Myeloperoxidase (MPO) activity and gene expression increased significantly in IRC and IRM-100 group rat ovarian tissue compared with the SG group (P < 0.0001). However, MPO activity and gene expression in IRM-200 group ovarian tissue decreased significantly compared with the IRC and IRM-100 groups (P < 0.0001). Histopathologically, pronounced congestion, dilated vessels, hemorrhage, edema, degenerative cells, and neutrophil migration and adhesion to the endothelium were observed in the IRC and IRM-100 group ovarian tissues. A small number of congested dilated vessels, mild congestion, and edema were observed in the IRM-200 group, but no neutrophil migration and adhesion to the endothelium or degenerative cells. Conclusions: At 200 mg/kg dose metamizole prevented ovarian injury induced with I/R. This data show that metamizole can be used in the ovarian I/R injury treatment.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2016Effects of nimesulide on the small intestine mucositis induced by methotrexate in rats
Arslan, Aynur; Ozcicek, Adalet; Suleyman, Bahadir; Coban, Taha Abdulkadir; Cimen, Ferda Keskin; Nalkiran, Hatice Sevim; Kuzucu, Mehmet; Altuner, Durdu; Cetin, Nihal; Suleyman, Halis
Intestinal mucositis is one of the major problems in the patients receiving cancer treatment. Nimesulide is a drug with antioxidant, antiinflammatory and antiulcer features. We aimed to investigate the effect of nimesulide on the small intestine mucositis induced by methotrexate (MTX) in rats. Experimental animals were divided into the control group, MTX group (MTXG) and nimesulide+MTX administered group (NMTXG) with eight rats per group. The control and MTXG groups were given distilled water by gavage and the NMTXG was given nimesulide 100 mg/kg orally. After one hour, the NMTXG and MTXG rat groups were administered oral MTX 5 mg/kg. This procedure was repeated once a day for 15 days and the rats were sacrificed. The duodenum and jejunum of each rat was removed for the assessment of biochemical markers and histopathological evaluation. Malondialdehyde (MDA) and myeloperoxidase (MPO) levels were significantly higher in the duodenal and jejunal tissues of the animals which received MTX, compared to the control and NMTXG (P<0.001). Also, the levels of total glutathione (tGSH), glutathione reductase (GSHRd), glutathione peroxidase (GSHPx), catalase (CAT) and superoxide dismutase (SOD) were significantly lower in the MTXG (P<0.001) compared to other groups. MTX led to villus and crypt epithelial damage and inflammation containing marked PMNL and eosinophils in the intestinal tissues histopathologically. Whereas, there was only mild irregularities in the villus structures of the NMTXG. Nimesulide protected the small intestines against damage by MTX. Intestinal mucositis caused by MTX may be preventable by co-administered nimesulide.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2016Can thıamıne pyrophosphate prevent desflurane ınduced hepatotoxıcıty ın rats?
Arslan, Aynur; Kuyrukluyildiz, Ufuk; Binici, Orhan; Cetin, Nihal; Balci, Mecdi Gurhan; Kuzucu, Mehmet; Yilmaz, Adnan; Altuner, Durdu; Coban, Taha Abdulkadir
PURPOSE: To investigate the effects of thiamine pyrophosphate (TPP) against desflurane induced hepatotoxicity. METHODS: Thirty experimental animals were divided into groups as healthy (HG), desflurane control (DCG), TPP and desflurane group (TDG). 20 mg/kg TPP was injected to intraperitoneally TDG. After one hour of TPP administration, desflurane was applied for two hours. After 24 hours, liver tissues of the animals killed with decapitation were removed. The oxidant/antioxidant levels and ALT, AST and LDH activities were measured. The histopathological examinations were performed in the liver tissues for all rats. RESULTS: Notwithstanding the levels of oxidants and liver enzymes were significantly increased (p<0.0001), antioxidant levels were significantly decreased in DCG (p<0.0001). On contrary to the antioxidant parameters were increased (p<0.05) the oxidant parameters and liver enzymes were decreased in TDG (p<0.0001). Whereas multiple prominent, congestion, hemorrhage and dilatation were observed in sinusoids and lymphocyte-rich inflammation results in the centrilobular and portal areas of liver tissue in DCG, these findings were observed less frequently in TDG. CONCLUSİON: Thiamine pyrophosphate prevented liver oxidative damage induced with desflurane and may be useful in prophylaxis of desflurane induced hepatotoxicity.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2016Liv-52; prophylactic or therapeutic agent against desflurane-induced hepatotoxicity in rats
Kuyrukluyildiz, Ufuk; Binici, Orhan; Cetin, Nihal; Balci, Mecdi G.; Onk, Didem; Cankaya, Murat; Kafa, A. Humeyra Taskin; Altuner, Durdu; Yilmaz, Adnan
Liv-52 has hepatoprotective effect and may be beneficial for desflurane hepatotoxicity. We aimed to investigate the prophylactic or therapeutic effects of Liv-52 on hepatotoxicity induced desflurane. The study was designed into seven groups as healthy group (HG), desflurane control groups (DCG-1, DCG-2, and DCG-3), Liv-52 treatment after desflurane (DLG), Liv-52 treatment before desflurane group (LDG), and Liv-52 treatment before and after desflurane group (LDLG). Desflurane was adjusted as to 6% concentration for two hours. Liv-52 was given 20 mg/kg. Alanine transaminase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), malondialdehyde (MDA), and total glutathione (tGSH) levels were measured. MDA, tGSH, ALT, AST, and LDH levels of DLG, LDG, and LDLG were found statistically significant compared to controls and healthy groups (p < 0.05). But the differences between HG and LDLG for both MDA, tGSH, ALT, AST, and LDH parameters were found statistically insignificant (p > 0.05). Pathological findings such as sinusoidal dilatation congestion and inflammation areas were observed in control groups but not in LDLG. Both prophylactic and therapeutic uses of Liv-52 may be the most appropriate methods to be minimized desflurane-induced hepatotoxicity.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2016Gene expression and histopathological evaluation of thiamine pyrophosphate on optic neuropathy induced with ethambutol in rats
Cinici, Emine; Cetin, Nihal; Suleyman, Bahadir; Altuner, Durdu; Yarali, Oguzhan; Balta, Hilal; Callk, Ilknur; Tumkaya, Levent; Suleyman, Halis
AIM: To compare the effects of thiamine pyrophosphate (TPP) and thiamine (TM) in oxidative optic neuropathy in rats induced by ethambutol. METHODS: The animals were divided into four groups: a control group (CG), an ethambutol control (ETC) group, TM plus ethambutol group (TMG), and TPP plus ethambutol group (TPPG). One hour after intraperitoneal administration of TM 20 mg/kg to the TMG group and TPP 20 mg/kg to TPPG group, 30 mg/kg ethambutol was given via gavage to all the groups but the CG. This procedure was repeated once daily for 90d. After that period, all rats were exposed to high levels of anaesthesia in order to investigate the gene expression of malondialdehyde and glutathione in removed optic nerve tissue and histopathologically to examine these tissues. RESULTS: Malondialdehyde gene expression significantly increased, whereas glutathione gene expression significantly decreased in the ETC group compared to the CG. TM could not prevent the increase of malondialdehyde gene expression and the decrease of glutathione, while TPP significantly could suppress. Histopathologically, significant vacuolization in the optic nerve, single-cell necrosis in the glial cells, and a decrease in oligodendrocytes were observed in the ETC group. Vacuolization in the optic nerve, a decrease in oligodendrocytes and single-cell necrosis were found in the TMG group, while no pathological finding was observed in the TPPG group except for mild vacuolization. CONCLUSION: TPP protects the optic nerve against the ethambutol-induced toxicity but TM does not. TPP can be beneficial in prophilaxis of optic neuropathy in ethambutol therapy.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2016Effect of thiamine pyrophosphate on retinopathy induced by hyperglycemia in rats: A biochemical and pathological evaluation
Cinici, Emine; Ahiskali, Ibrahim; Cetin, Nihal; Suleyman, Bahadir; Kukula, Osman; Altuner, Durdu; Coban, Abdulkadir; Balta, Hilal; Kuzucu, Mehmet; Suleyman, Halis
Purpose: Information is lacking on the protective effects of thiamine pyrophosphate (TPP) against hyperglycemia-induced retinopathy in rats. This study investigated the biochemical and histopathological aspects of the effect of TPP on hyperglycemia-induced retinopathy induced by alloxan in rats. Materials and Methods: The rats were separated into a diabetic TPP-administered group (DTPG), a diabetes control group (DCG) and a healthy group (HG). While the DTPG was given TPP, the DCG and HG were administered distilled water as a solvent at the same concentrations. This procedure was repeated daily for 3 months. At the end of this period, all of the rats were euthanized under thiopental sodium anesthesia, and biochemical and histopathological analyses of the ocular retinal tissues were performed. The results of the DTPG were compared with those of the DCG and HG. Results: TPP prevented hyperglycemia by increasing the amount of malondialdehyde and decreasing endogen antioxidants, including total glutathione, glutathione reductase, glutathione S-transferase and superoxide dismutase. In addition, the amounts of the DNA oxidation product 8-hydroxyguanine were significantly lower in the retinas of the DTPG compared to the DCG. In the retinas of the DCG, there was a marked increase in vascular structures and congestion, in addition to edema. In contrast, little vascularization and edema were observed in the DTPG, and there was no congestion. The results suggest that TPP significantly reduced the degree of hyperglycemia-induced retinopathy. Conclusions: The results of this study indicate that TPP may be useful for prophylaxis against diabetic retinopathy.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2016The effect of thiamine pyrophosphate on ethambutol-induced ocular toxicity
Cinici, Emine; Cetin, Nihal; Ahiskali, Ibrahim; Suleyman, Bahadir; Altuner, Durdu; Alp, Hamit Hakan; Sener, Ebru; Calik, Ilknur; Suleyman, Halis
Context: Ethambutol-induced retinal oxidative damage in patients with tuberculosis is still not being adequately treated. The protective effect of thiamine pyrophosphate against oxidative damage in some tissues has been reported, but no information on the protective effects of thiamine pyrophosphate against ethambutol-induced oxidative retinal damage has been found in the medical literature. Objective: The objective is to investigate whether thiamine pyrophosphate has a protective effect against oxidative retinal damage in rats induced by ethambutol. Materials and methods: Experimental animals divided into four groups (n = 10): the healthy group (HG), the ethambutol control group (EMB), thiamine + ethambutol group (Thi–EMB) and thiamine pyrophosphate + ethambutol group (TPP–EMB). The rats in the TPP–EMB and Thi–EMB groups were administered thiamine pyrophosphate and thiamine, respectively, at doses of 20 mg/kg intraperitoneally. Distilled water was administered intraperitoneally to the HG and the EMB groups as a solvent in the same volumes. One hour after drug injection, 30 mg/kg ethambutol was administered via an oral gavage to the TPP–EMB, Thi–EMB and EMB groups. This procedure was repeated once a day for 90 days. At the end of this period, all rats were euthanized under high-dose thiopental sodium anesthesia, and biochemical and histopathological investigations of the retinal tissue were performed. Results: Malondialdehyde (MDA) and DNA damage product 8-hydroxyguanine levels were significantly lower in the retinal tissue of TPP–EMB and HG groups compared to those of the Thi–EMB and EMB groups, and total glutathione (tGSH) was also found to be higher. In addition, severe retinal tissue vascularization, edema and loss of ganglion cells were observed in the Thi–EMB and EMB groups, whereas histopathological findings for the TPP–EMB group were observed to be close to normal. Discussion and conclusion: These findings suggest that thiamine pyrophosphate protects retinal tissues from ethambutol-induced oxidative damage, and thiamine does not. This positive effect of thiamine pyrophosphate may be useful in the prevention of ocular toxicity that occurs during ethambutol use.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2015A comparative investigation of the analgesic effects of metamizole and paracetamol in rats
Ince, Ilker; Aksoy, Mehmet; Ahiskalioglu, Ali; Comez, Mehmet; Dostbil, Aysenur; Celik, Mine; Yilmaz, Ismayil; Mammadov, Renad; Dogan, Hasan; Boztok Ozgermen, Basak
Background: This study investigated the effects of metamizole and paracetamol on pain and oxidative stress induced by scalpel incision and carrageenan in rats. Materials and methods: Total of 144 rats were divided into groups of 12 animals. Six groups each were used for scalpel incision and carrageenan tests. Pain was inflicted by applying a scalpel incision or carrageenan. Pain-created groups by scalpel incision received metamizole (SIM) or paracetamol (SIP) at doses of 250 or 500 mg/kg. Pain-created groups by carrageenan received metamizole (CAM) or paracetamol (CAP) at doses of 250 or 500 mg/kg. Analgesic activity was determined by Basile Algesimeter. The COX-2 and MPO gene expressions were determined, and malondialdehyde and tGSH were measured in rat paws. Results: In the scalpel incision test, pain was reduced in groups of SIM-250 and SIM-500 in the first hour by 65.2% and 91.3%, respectively, and in the third hour by 51.9% and 77.8%, respectively, compared with the SIC group. In SIP-250 and SIP-500 groups, pain was reduced in the first hour by 43% and 74%, respectively, and by 33.4% and 59.3%, respectively, in the third hour compared with the SIC group. In the carrageenan test, in groups CAM-250 and CAM-500, pain was reduced in the first hour by 72.3% and 86.1%, respectively, and by 65.8% and 71.4%, respectively, in the third hour compared with the CCG group. In groups CAP-250 and CAP-500, pain was reduced in the first hour by 52.8% and 69.4%, respectively, and by 28.6% and 25.8%, respectively, in the third hour compared with the CCG group. Metamizole inhibited COX-2 gene expression at a dose of 500 mg/kg in the carrageenan test. At doses of 250 and 500 mg/kg, metamizole reduced COX-2 and MPO gene expressions and oxidative stress induced by scalpel incision or carrageenan. But both doses of paracetamol were unable to suppress that parameters. Conclusions: Our results show that metamizole is more effective than paracetamol for treating surgical trauma-related pain, inflammation, and oxidative stress and hence may be a preferential drug to paracetamol.
RDL Kalıcı Kaydıİncele - ArticleYayıncıda açık erişim2015Effect of disulfiram on ketamine-induced cardiotoxicity in rats
Cetin, Nihal; Suleyman, Bahadir; Altuner, Durdu; Kuyrukluyildiz, Ufuk; Ozcicek, Fatih; Coskun, Resit; Kurt, Nazahat; Suleyman, Halis
It is known that ketamine increases the production of catecholamines, causing oxidative damage to the heart. Suppression of the production of catecholamines by disulfiram, a drug with antioxidant properties, indicates that disulfiram may decrease ketamine-induced cardiotoxicity. The objective of the present study was to investigate the effect of disulfiram on ketamine-induced cardiotoxicity in rats. Disulfiram was administered by oral gavage in doses of 25 mg/kg to rats in the DK-25 group and 50 mg/kg to rats in the DK-50 group. Distilled water was applied in the ketamine control (KC) and healthy (HG) rat groups. At one hour after drug administration and subsequently at ten-minute intervals, a 60 mg/kg dose of ketamine was intraperitoneally injected in the rats in all groups other than HG, and anesthesia was maintained for three hours. Disulfiram prevented both increase in the levels of parameters indicating oxidative and myocardial damage and decrease of antioxidant levels in the heart tissue with ketamine in a dose-dependent manner. Disulfiram better prevented occurrence of cardiotoxicity with ketamine in the 50 mg/kg dose than in the 25 mg/kg dose. It is concluded that disulfiram may usefully be applied in clinical practice in the prevention of cardiotoxicity as observed during anesthesia with ketamine.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2015The molecular mechanism involved in the protective effect of etodolac against ovarian injury induced with ischemia/reperfusion in rats
Al, Ragip A.; Yapca, Omer E.; Dogan, Hasan; Mammadov, Renad; Altuner, Durdu; Suleyman, Bahadir; Cetin, Nihal
This study investigated the protective effect at the molecular level of etodolac against ovarian injury induced in rats with ischemia/reperfusion (I/R). Rats were divided into four groups. The 25 mg/kg etodolac + ovarian ischemia/reperfusion (EIR 25) and 50 mg/kg etodolac+ovarian ischemia/reperfusion (EIR 50) groups were given etodolac by the oral route 1 h before administration of anesthesia. Two h of ischemia and 2 h of reperfusion were applied to ovarian tissue in all groups (except for (SG) sham operation group) 1 h after etodolac administration. The experimental results showed that etodolac at a dose of 50 mg/kg suppressed MPO (myeloperoxidase), TNF-α, IL-1β gene expression in ovarian tissue with I/R better than at a dose of 25 mg/kg. In addition, etodolac at these doses prevented a decrease in SOD gene expression in ovarian tissue subjected to I/R to a significant extent (p < 0.0001). In conclusion, etodolac can be used in the treatment of I/R-related ovarian injury.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2015The relation between the effect of a subhypnotic dose of thiopental on claw pain threshold in rats and adrenalin, noradrenalin and dopamine levels
Aksoy, Mehmet; Ahiskalioglu, Ali; Ince, Ilker; Celik, Mine; Dostbil, Aysenur; Kuyrukluyildiz, Ufuk; Altuner, Durdu; Kurt, Nezahat; Suleyman, Halis
Thiopental sodium (TPS) needs to be applied together with adrenalin in order to establish its analgesic effect in general anesthesia. We aimed to investigate the effect of TPS on the claw pain threshold in rats and evaluated its relationship with endogenous adrenalin (ADR), noradrenalin (NDR), and dopamine (DOP) levels. Intact and adrenalectomized rats were used in the experiment. Intact animals were divided into the following groups: 15 mg/kg TPS (TS), 0.3 mg/kg ADR+15 mg/kg TPS (ATS) and 0.3 mg/kg ADR alone (ADR). Adrenalectomized animals were divided into the following groups: 15 mg/kg TPS (A-TS), 0.3 mg/kg ADR+15 mg/kg TPS (A-ATS) and 0.3 mg/kg ADR alone (A-ADR). Claw pain threshold and blood ADR, NDR, and DOP levels were measured. The TS group’s claw pain threshold was found low. However, the claw pain thresholds of the ATS and ADR groups increased significantly. In the A-TS group, the pain threshold decreased compared with normal, and in the A-ATS and A-ADR groups, the pain threshold increased. TPS reduced the blood ADR levels in intact rats; however, no significant changes were observed in the NDR and DOP levels. #TPS provides hyperalgesia by reducing the production of ADR in rats. The present study shows that to achieve analgesic activity, TPS needs to be applied together with ADR.
RDL Kalıcı Kaydıİncele - ArticleYayıncıda açık erişim2015Protective effect of resveratrol against methotrexate-induced oxidative stress in the small intestinal tissues of rats
Arslan, Aynur; Ozcicek, Fatih; Cimen, Ferda Keskin; Altuner, Durdu; Yarali, Oguzhan; Kurt, Nezahat; Tumkaya, Levent; Ozturk, Cengiz; Suleyman, Halis
The effect of resveratrol on the damage induced by methotrexate (MTX) in rat duodenum and jejunum tissue was investigated and evaluated in comparison with famotidine. The rats were divided into four groups as healthy group (HG), resveratrol+MTX (RMTX) group, famotidine+MTX (FMTX) group and the control group which received MTX (MTXC). RMTX group was given resveratrol 25 mg/kg and FMTX group famotidin 25 mg/kg, while MTXC and HG groups were orally administered distilled water once a day for 30 days. The rats in RMTX, FMTX and MTXC groups were given MTX of 5 mg/kg dose by the same way for 30 days. At the end of this period, amount of MDA, 8-OH/Gua and tGSH, and MPO gene expression were measured in the duodenal and jejunal tissues and the results were histopathologically evaluated. Resveratrol and famotidine were found to significantly prevent elevation of the MDA, 8-OH/Gua and MPO parameters with MTX and decrease of the levels of tGSH in the duodenal and jejunal tissues. Both drugs prevented severe damage to the villus and crypt epithelium in the duodenum and jejunum, congestion and hemorrhage, inflammatory cell infiltration and necrosis in the mucosa and submucosa due to MTX administration. Resveratrol could be considered in the clinical practice for treatment of the tissue damage in the intestines due to use of MTX, in comparison with famotidine. Resveratrol may be more advantageous than famotidine in long-term use against MTX toxicity since it does not inhibit gastric acid secretion.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2015The Effect of Etoricoxib on Hepatic Ischemia-Reperfusion Injury in Rats
Kunak, Celalettin Semih; Kukula, Osman; Mutlu, Emre; Genç, Fatma; Güleç Peker, Gülçer; Kuyrukluyildiz, Ufuk; Binici, Orhan; Altuner, Durdu; Alp, Hamit Hakan
Ischemia-reperfusion (I/R) damage is known to be a pathological process which continues with the increase of oxidants and expands with the inflammatory response. There is not any study about protective effect of etoricoxib on the liver I/R damage in literature. Objective. This study investigates the effect of etoricoxib on oxidative stress induced by I/R of the rat liver. Material and Methods. Experimental animals were divided into four groups as liver I/R control (LIRC), 50 mg/kg etoricoxib + liver I/R (ETO-50), 100 mg/kg etoricoxib + liver I/R (ETO-100), and healthy group (HG). ETO-50 and ETO-100 groups were administered etoricoxib, while LIRC and HG groups were orally given distilled water by gavage. Hepatic artery was clamped for one hour to provide ischemia, and then reperfusion was provided for 6 hours. Oxidant, antioxidant, and COX-2 gene expressions were studied in the liver tissues. ALT and AST were measured. Results. Etoricoxib in 50 and 100 mg/kg doses changed the levels of oxidant/antioxidant parameters such as MDA, MPO, tGSH, GSHRd, GST, SOD, NO, and 8-OH/Gua in favour of antioxidants. Furthermore, etoricoxib prevented increase of COX-2 gene expression and ALT and AST levels. This important protective effect of etoricoxib on the rat liver I/R can be tested in the clinical setting.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2014The effect of Hippophae rhamnoides extract on oxidative damage on rat's gastric tissue depending on co-implementation of methotrexate and indomethacin
Yilmaz, Ismayil; Demiryilmaz, Ismail; Sener, Ebru; Cetin, Nihal; Ucuncu, Yilmaz; Altuner, Durdu; Kurt, Nezahat; Kunak, Semih C.; Kisaoglu, Abdullah; Ozogul, Bünyami
The effect of Hippophae rhamnoides (HR) extract on oxidative damage on rat's gastric tissue depending on co-implementation of methotrexate (MTX) and indomethacin (IND) was investigated biochemically and histopathologically. HR extract was given to HR extract-given (HRIM) group of rats with dose of 50 mg/kg, and to IND+MTX-implemented rat group (IMG) orally with distilled water through catheter. One hour after, IND and MTX combination was given to HRIM and IMG groups orally for 7 days with dose of 5 mg/kg. In gastric tissues of IMG group, the MDA amount increased to healthy-sham (SG) and HRIM groups. The amount of tGSH, in IMG group's gastric tissue decreased to SG and HRIM groups. Serious focal erosions, sub-mucosal edemas and mixed-type inflammatory cell infiltrations have been observed on surface epithelium of histopathological sections of stomachs of IMG group. HR extract prevent the gastric damage in rats' gastric tissues, which was formed by MTX and IND combination.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2013The protective effect of melatonin and agomelatin against cisplatin-induced nephrotoxicity and oxidative stress in the rat kidney
Yilmaz, Ismayil; Demiryilmaz, Ismail; Turan, Mehmet I.; Suleyman, Bahadir; Turan, Isil S.; Altuner, Durdu; Alp, Hamit H.; Suleyman, Halis
Cisplatin is used to treat various types of cancers. Its use is limited, however, due to nephrotoxicity, which may result from free radical damage. Evidence exists that melatonin reduces oxidative stress-induced damage. This study investigated the protective effect of agomelatin, a melatonin receptor agonist, against cisplatin-induced nephrotoxicity and oxidative stress in the rat kidney. Groups of rats were given cisplatin with or without agomelatin or melatonin, or distilled water for 14 days. MDA, tGSH, MPO and 8-OH Gua levels were measured to determine oxidative and DNA damage in renal tissue. Levels of MDA, MPO and 8-OH Gua were lower in the Mel+Cis and Ago+Cis groups than in the Cis group (P < 0.001, P < 0.001, and P < 0.05, respectively). The tGSH level in the Mel+Cis group was higher than that in the Cis group (P < 0.001). Agomelatin and melatonin thus reduced cisplatin-induced oxidative damage and DNA damage in the rat kidney. This suggests that melatonin may be effective in preventing cisplatin nephrotoxicity.
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