Sürdürülebilir Kalkınma Amaçları
Yayınlar
- ArticleAçık Erişim2024Investigation of the Knowledge Levels of Physicians Working in the Emergency Department About Testicular Torsion: Observational Study
Keskin, Ercüment; Gul, Abdullah; Bozkurt, Aliseydi; Ekici, Ozgur; Emre; Doğan, Muhammet Emin; Balıkçı, Ayşenur
Objective: The aim of the study is to investigate the level of knowledge of emergency physicians about the clinical approach to testicular torsion. Material and Methods: A questionnaire with 10 questions was prepared to determine the level of knowledge about acute scrotum. 4 questions consisted of demographic data and 10 questions consisted of questions about clinical knowledge. Four of these questions were specifically measuring physical examination (PE) knowledge. The total scores of the answers given and the answers to the questions measuring PE knowledge were recorded separately, each question being one score. Total scores were compared according to gender, institution and degree. Results: A total of 99 emergency physicians were reached for the study. Mean age 27.6±4.6 years; male/female ratio was 55/44. The mean total score of all physicians was 6.13±1.63; The mean total score of the 4-question PE was 2.29±1.07. Physicians participating in the survey were separated according to their gender, institution, and degree, and their total scores were compared. No statistically significant difference was observed between the groups. While no statistically significant difference was observed in PE scores according to gender and the institutions they work at, there was a statistically significant difference between specialist physicians (3.09±1.04) and general practitioners (1.94±0.95) when compared according to their degrees (p: 0.009). Conclusion: It is important to increase the PE knowledge of the physicians working in the emergency department about testicular torsion through continuous education after graduation in order to prevent organ losses.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024The relationship between serum adropin levels and erectile dysfunction
Keskin, Ercument; Ekici, Ozgur; Erdogan, Abdullah; Bozkurt, Aliseydi; Sambel, Murat; Karabakan, Mehmet; Mertoglu, Cuma
Erectile dysfunction (ED) is associated with endothelial damage, especially atheroscle-rosis. The search for biomarkers that can predict ED still continues. Adropin is known to affect nitric oxide (NO) bioavailability and energy homeostasis. In our study, we have aimed to investigate the relationship between serum adropin levels and ED. Male patients with and without ED between 40–60 years of age, who presented to the outpatient clinics of urology between November 2019–February 2020, were prospectively included in the study. Biochemical values measured at the time of admission to the outpatient clinic. According to the International Index of Erectile Function-5 (IIEF-5) scores which range between 5 and 25 points, patients with a score ≤21 were considered to have ED. The patients were divided into two groups: as ED and non-ED control groups. Laboratory values obtained at admission to outpatient clinics of urology were compared between groups. Patients with (n: 40), and without (n: 40) ED were included in the study. The mean age (50.2 ± 5.7 years), average body mass index (BMI) (29.7 ± 2.5 kg/m2 ), IIEF score (15.8 ± 6 pts), serum adropin (584.8 ± 172 pg/mL), and total testosterone (396.4 ± 91.7 ng/dL) levels were recorded. Serum adropin and testosterone levels were statistically significantly higher in the non-ED group than in the ED group (712.3 ± 222 pg/mL vs. 511.1 ± 145 pg/mL, p < 0.001 and 420.5 ± 56 ng/dL vs. 374.3 ± 98 ng/dL, respectively p = 0.032). Whereas fasting blood glucose (FBG) values were found to be statistically significantly higher in the ED group (100.2 ± 14 mg/dL vs. 143.8 ± 78 mg/dL, p = 0.001). According to the results of our study, serum levels of adropin which improves endothelial functions were comparatively lower in ED patients, as expected.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023Retrograd İntrarenal Cerrahide Üreteral Erişim Kılıfı Kullanımının Postoperatif Sonuçlara Etkisi: Retrospektif Kohort Çalışması
Keskin, Ercüment; Gul, Abdullah; Caglayan, Volkan; Bozkurt, Aliseydi; Ekici, Ozgur; Erdogan, Abdullah; Emre; Altunok, Vedat
Amaç: Bu çalışmada, fleksibl üreterorenoskopi (f-URS) s ıra- sında yaygın olarak kullanılan üreteral erişim kılıfının (ÜEK) postope- ratif enfektif komplikasyonlar ve böbrek fonksiyonları üzerine etkilerini araştırmayı amaçladık. Gereç ve Yöntemler: Retrospektif yöntem ile Ocak 2021-Temmuz 2023 tarihleri aras ında böbrek ya da proksimal üreter taşı nedeniyle f-URS yap ılmış olan hastaların verileri tarand ı. Hastaların ameliyat süreleri ve erişim kılıfı kullanım durumları, preo- peratif görüntüleri ve postoperatif ate ş, sepsis, üriner sistem enfeksi- yonu (ÜSE) durumları ve periferik kandan ve idrar tetkikinden bakılan inflamasyon belirteçleri kaydedildi. Hastaların kreatinin ve glomerüler filtrasyon hız (GFH) değişimleri ile taburcu olduktan sonra ilk 2 hafta- daki acil servis başvuruları kaydedildi. Hastalar ÜEK kullanılmayanlar ve kullanılanlar olarak 2 gruba ayrıldı. Gruplar arasında kaydedilen ve- riler kar şılaştırıldı. Bulgular: Çalışmaya toplamda 124 hasta dâhil edildi. Hastaların ortalama yaşı 48,15±17,06 yıl idi. Hastalar ÜEK kul- lanılmayan (Grup 1, n=70) ve kullanılan (Grup 2, n=54) olarak 2 gruba ayrıldı. Her iki grup arasında cinsiyet, taş tarafı, double J stent (DJS) ta- kılması, postoperatif sepsis, postoperatif ÜSE, postoperatif lökosit es- teraz pozitifli ği, postoperatif hematüri ve postoperatif acil servise başvuru açısından istatistiksel olarak anlaml ı farklılık izlenmedi. Po- stoperatif ateş, idrar tetkikinde nitrit pozitifliği, beyaz kan hücresi ve C- reaktif protein erişim kılıfı kullanılmayan hasta grubunda istatistiksel olarak anlamlı yüksek bulundu. Her iki grup aras ında ortalama ya ş, ameliyat süresi, postoperatif DJS çekim günü, kreatinin ve GFH deği- şimleri açısından istatistiksel olarak anlamlı farklılık izlenmedi. Sonuç: Böbrek taşı hastalarında erişim kılıfı kullanmanın böbrek fonksiyon- ları üzerinde koruyucu etkisi gözlenmemekle birlikte, postoperatif en- fektif komplikasyonları bir miktar azaltabilir.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023Comparison of serum Kim-1 and Miox levels in patients that underwent percutaneous nephrolithotomy and flexible ureterorenoscopy
Altun, Abdulsemet; Bozkurt, Aliseydi; Erdogan, Abdullah; Mertoğlu, Cuma; Hirik, Erkan; Keskin, Ercüment; Turan, Abdullah
Objective: Percutaneous nephrolithotomy (PNL) and retrograde intrarenal surgery (RIRS) are common surgical methods in the treatment of kidney stones. Possible effects on kidneys are an important factor in determining the surgical procedure and the surgical method. In our study, kidney injury molecule-1 (KIM-1) and myo-inositol oxygenase (MIOX) were used to compare acute kidney injury in patients that underwent PNL and RIRS. Material and Method: Eighty patients aged 20–75, who underwent PNL or RIRS in our urology clinic between November 2018 and February 2020 were included in the study. In this prospective study, the demographic characteristics, stone size, operation time, preoperative and postoperative hemoglobin and biochemistry values of the patients were recorded. About 5 cc blood samples taken from the patients before the operation and at the fourth hour after the operation were centrifuged and kept at −80°C, and the KIM-1 and MIOX levels were measured in the biochemistry department. Results: There was no difference between the groups in terms of demographic data; however, the operation time and length of hospital stay were significantly longer in the PNL group. The mean increase in MIOX was 10.583 ± 9.73 and 7.501 ± 16.46 ng/ml in the PNL and RIRS groups, respectively. Although there was a statistically significant increase in both groups, this increase was greater in the PNL group. A significant increase was observed only in the PNL group in the postoperative period (p = 0.003). Discussion and Conclusion: The findings of the study suggest that the PNL procedure causes more damage to the kidneys than RIRS.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2022The effects of two different antioxidant combinations on sperm parameters
Yaris, Mehmet; Akdogan, Nebil; Öztürk, Murat; Bozkurt, Aliseydi; Karabakan, Mehmet
Background: Studies demonstrate that getting antioxidants in the course of treatment has a positive impact beneficial effect on fertility, especially on the quality of sperm. Because of that reason antioxidants are recommended as a potentially influential treatment for infertility in men. However, it is argued that this treatment is not based on sufficient evidence and has no effect on the rate of healthy pregnancy. Objective: In this study, two different antioxidant combinations with different doses and contents were evaluated in terms of their effect on sperm parameters. Materials/methods: A total of 122 patients diagnosed with idiopathic infertility were enrolled in our multicenter study. The patients were divided into two different groups: The first group used a combination 2 x 1 sachet form (l-carnitine 1 g, acetyl-l-carnitine 0.5 g, fructose 1 g, citric acid 0.50 mg, selenium 50 mu g, coenzyme Q10 20 mg, vitamin C 90 mg, zinc 10 mg, folic acid 200 mu g, and vitamin B12 1.5 mu g) and the second group used a combination tablets form 2 x 1 (l-carnitine 500 mg, selenium 50 mu g, coenzyme Q10 20 mg, vitamin C 60 mg, zinc 15 mg, folic acid 400 mu g, vitamin E, and ginseng 15 mu g) for 6 months. The total semen volume, the total sperm number, sperm concentration, sperm motility, and lastly morphological findings of the patients were compared at the end of 6 months. Results: The mean age of the patients participating in the study was 30.8 +/- 6.05 years. No significant difference was found between the two groups in terms of baseline sperm count. There was a significant difference between the baseline and sixth-month values of the patients using both combinations. However, no significant statistical difference was found between the groups according to the sixth-month data. The combinations of both antioxidants had a positive effect on sperm parameters, and the use of different doses and contents had a similar effect. Conclusion: Both antioxidants respectively had a positive effect on sperm parameters and also the use of different doses and contents had a similar effect.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2022Qualification and quantification of plasma cell-free DNA after long-term storage conditions in patients with benign prostatic hyperplasia (BPH): A pilot study
Bozkurt, Aliseydi; Gürbüzel, Mehmet; Sayar, Ilyas; Baydeniz, Soner; Arslan, Yusuf Kemal
Objectives: Free DNA is used as a cancer biomarker due to its low cost, high applicability, and fast, reliable results compared to invasive methods. This study aimed to evaluate the quantification of plasma-free DNA after long-term storage conditions and perform qualification through single nucleotide polymorphism (SNP) screening based on this DNA. Methods: Plasma-free DNA samples were quickly isolated from the peripheral blood of both the benign prostatic hyperplasia (BPH) and control group participants and then maintained at -80 °C for four years. Upon thawing, first, free DNA was purified and fluorometric measurements were taken to determine the amount of DNA. Subsequently, the rs6983267, rs12628, and rs1799939 SNPs were screened in the CCAT2, HRAS, and RET genes, respectively. Results: Significant results were obtained from the fluorometric measurements in terms of single-stranded DNA (ssDNA) (p<0.001). However, there was no significant difference in SNPs rs6983267, rs12628, and rs1799939 in the BPH group compared to the healthy individuals. Conclusions: The data show that fluorometric ssDNA measurements are suitable for quantifying free DNA. The fact that SNP screening can be done successfully in both healthy people and BPH patients suggests that plasma-free DNA can be stored in the laboratory under appropriate conditions.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2021Quantification of cell-free DNA from bladder cancer patients after long-term storage and its use in determining CCAT2, HRAS, and RET polymorphism: A pilot study
Arslan, Yusuf Kemal; Sayar, Ilyas; Gürbüzel, Mehmet; Bozkurt, Aliseydi; Baydeniz, Soner Baydeniz
Aim: The level of free DNA is used as a cancer biomarker due to having several advantages related to lower cost, easy applicability, and rapid results compared to invasive methods. Materials and Methods: This study investigated whether or not free DNA functions as intended when stored. The free DNA isolated for this purpose was kept at –80 degrees for four years. Then, the level of free DNA was measured in patients with bladder cancer by both photometric and fluorometric methods, and it was investigated whether this biomarker was effective in determining Single Nucleotide Polymorphisms (SNP). The gene names and the reference numbers of the locations where polymorphism was expected were as follows: CCAT2 (rs6983267), HRAS (rs12628), and RET (rs1799939). Results: According to the results, having the T allele at rs12628 increases the risk of disease by 2.6 times (OR: 2.60; 95% CI: 1.10– 6.10) and having the G allele at rs1799939 increases the risk of disease 2.7 times (OR: 2.70; 95% CI: 1.12–6.69). Conclusions: The general findings showed that fluorometric measurements were more advantageous when considering the sensitivity of free DNA measurements. It was also concluded that, if treated rapidly and conserved in appropriate laboratory conditions, free DNA can be effectively used in SNP studies. This study provides important data to demonstrate the quality of free DNA.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2020Can we contribute to the diagnosis of diabetes and regulation of blood glucose by increasing the urologists' awareness of glucosuria?
Erdogan, Abdullah; Bozkurt, Aliseydi; Altun, Abdulsemet; Turan, Abdullah
Aims: Diabetes mellitus is a progressive, chronic, systemic, metabolic disease that must be managed effectively. Its prevalence is increasing rapidly. We investigated whether urologists' awareness and recognition of glucosuria contributed to the diagnosis of diabetes and regulation of blood glucose. Methods: A total of 39,053 patients were retrospectively evaluated between January 2018 and February 2019. Of them, 16,211 had undergone urinalysis for varied reasons. Glucosuria was semi-quantitatively measured as (+), (++), (+++), and (++++). Patients were assessed in terms of whether they had been referred to endocrinology or internal medicine departments within the 15 days and the presence/absence of a previous or new diagnosis of diabetes mellitus by measuring blood glucose and HgbA1c levels. Results: Glucosuria was detected in 665 patients (4.1%), of whom 495 were included in the study. 417 (84.2%) had been previously diagnosed with diabetes mellitus, and 56 (11.3%) newly received a diabetes mellitus diagnosis. Blood glucose and HbA1c values were normal in 22 (4.4%) patients with glucosuria. HgbA1c value was determined as 7 or above in 381 (91.3%) of 417 cases with a previous diabetes mellitus diagnosis. Conclusion: Referring all patients detected to have glucosuria in the spot urine test at any time regardless of fasting/non-fasting to the relevant departments can contribute to the diagnosis and treatment of diabetes mellitus disease.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2020A new proof for the relationship between erectile dysfunction and atherosclerosis: fragmented QRS?
Karabakan, Mehmet; Bozkurt, Aliseydi; Saylam, Barıs; Kucuksu, Zafer; Hirik, Erkan; Kagan Aktas, Binhan
In patients with coronary artery disease (CAD), fragmented QRS (fQRS) is a practical marker of myocardial ischemia evaluated on electrocardiography (ECG).The current study investigated the connection between FQRS and erectile dysfunction (ED), which is commonly accepted as an early sign and independent predictor of CAD. The study consisted of a 190 ED patients; 47 of them having severe, 44 moderate, 49 mild-to-moderate, and 50 mild ED, and a control group of 51 otherwise healthy adult men. None of the participants had a history of cardiac disease. There was a significant difference among the groups in terms of fQRS; severe, moderate, and mild-to-moderate ED groups had significantly higher rates of fQRS presence on ECG (27.5%, 23.5%, and 22.5%, respectively) in comparison with those of mild ED and the control groups (13.7% and 12.7%, respectively) (p < 0.001). In patients presenting with ED complaints, a fQRS finding on a simple ECG recording may serve as a first sign of the presence of CAD. As far as we know, the present study is the first in the literature assessing the possible relationship between ED and fQRS. On the ECG, the existence of fQRS was considered to be connected with ED; thus, including this basic parameter into the clinical decisions for ED patients may initiate an extensive diagnostic approach for their underlying cardiovascular diseases.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2019Effects of melatonin treatment on the spermatogenesis and serum inflammatory cytokine levels in diabetic rats
Erol, Huseyin Serkan; Terzioğlu, Merve Aydın; Onk, Didem; Gürsul, Cebrail; Gumustas, Sinem; Bozkurt, Aliseydi; Karabakan, Mehmet
Aim: Diabetes mellitus (DM) is known to have side effects on sexual and reproductive functions in males. The aim of current study was to investigate the effect of melatonin treatment on spermatogenesis and serum inflammatory cytokine levels in diabetic rats.Material and Method: Eighteen adult Wistar albino male rats were divided randomly into three groups each containing 6 rats; control group (group 1), diabetic group (group 2), diabetic treated with melatonin group (group 3). Experimental diabetes was obtained by administrating 50 mg/kg streptozotocin. Melatonin treatment was administered intraperitoneally for 7 days at a dose of 20 mg, daily. Serum levels of interleukin-1 beta (IL-1β), interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF-α) were assayed. Spermatogenesis was evaluated according to Johnsen score at immunopathological examination.Results: The Johnsen scores in control, diabetic and treatment groups were 9.78, 8.91, and 9.61, respectively. Spermatogenesis was negatively affected in diabetic rats and significantly improved with melatonin therapy (p<0.05). The serum levels of TNF-α in diabetic rats were observed to be increased compared to the control group (p<0.05). The levels of serum IL-1β and TNF- α were significantly decreased with melatonin treatment. IL-6 levels were not different among the three groups (p=0.248).Conclusions: In the present study, intraperitoneal melatonin treatment was determined to have a positive effect of on spermatogenesis and caused a decrease in serum inflammatory cytokine levels in diabetic rats.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2019How to prevent unnecessary request prostate-specific antigen testing?
Mertoğlu, Cuma; Bozkurt, Aliseydi
Aim: In this study, it was aimed to show how to limit the unnecessary requests of free prostate-specific antigen (fPSA) test changes with some regulations.Material and Methods: The fPSA requirements when total PSA values below 4 ng/mL or above 10 ng/mL were considered ‘unnecessary test request’. To do this, the relevant physicians were informed and the fPSA test was arranged to have a separate second window on the request panel and physicians were requested only tPSA test requests. An additional fPSA test was conducted by the laboratory staff from patients with a tPSA score of 4-10 ng / mL.Results: It was seen that 1236 fPSA and 1292 tPSA tests (fPSA/tPSA = 95.6%) were performed in our research hospital laboratory between January 17,.2017 and March 09,2017 while 328 fPSA and 1139 tPSA tests were also done between January 17, 2018 and March 09, 2018 (fPSA / tPSA = 28.7%). The ratio of sPSA test request to tPSA test request was found to be reduced by 66.9%.Conclusion: This study limited significant hospital expenditure and labor loss reducing the number of unnecessary fPSA tests with regulations made at the test prompt.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2019The evaluation of oxidative stress parameters in the benign prostatic hyperplasia, prostatitis and prostate cancer
Gürsul, Cebrail; Sayar, İlyas; Aydın, Merve; Çıkman, Aytekin; Bozkurt, Aliseydi; Karabakan, Mehmet
Aim: Recently, oxidative stress has been well known that it has especially important a role in benign prostatic hyperplasia, prostatitis, and prostate cancer. The present study has planned to estimate parameters of the oxidative stress and the antioxidant status in patients with benign prostatic hyperplasia, prostatitis and prostate cancer.Material and Method: For this aim totally fourty men with benign prostatic hyperplasia (n=14), prostatitis (n=15) and prostate cancer (n=11) without any other chronic disease, and who not use cigarettes and alcohol were subjected. Venous blood samples were taken. Thiobarbituric acid reaction (TBARS), total oxidant status (TOS), total antioxidant capacity (TAC), glutathione (GSH) levels, and catalase (CAT) activity in serum were assessed in patient with prostatitis, and prostate cancer compared to benign prostatic hyperplasia.Results: It was viewed that GSH levels were significantly lower, TBARS and TOS levels were significantly higher in patients with of prostate cancer compared to benign prostatic hyperplasia. Also, GSH and CAT significant different was determined in prostatitis compared to BPH. Oxidative stress may be concluded in prostate cancer and prostatitis, proved via the higher TBARS levels and lower GSH levels.Conclusion: The increased activity of antioxidant enzyme may be a compensatory regulation in response to oxidative stress.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2019Relationship Between Erectile Dysfunction, the Neutrophil-to-Lymphocyte Ratio, and the Platelet-to-Lymphocyte Ratio
Karabakan, Mehmet; Bozkurt, Aliseydi
Objective: Various chronic diseases, including hypertension (HT), diabetes mellitus (DM), and coronary artery disease (CAD), together with the medications used in the treatment of these conditions, are considered a part of the erectile dysfunction (ED) etiology. The neutrophil-to-lymphocyte ratio (NLR) and the platelet-to-lymphocyte ratio (PLR) provide information about many diseases. The NLR and PLR increase in systemic inflammation, certain gynecological and gastrointestinal cancers, CAD, various oncological diseases, and especially in acute coronary syndromes. In this study, the relationship between PLR, NLR, and ED severity were examined in patients with ED. Methods: All data from patients were screened retrospectively. Body mass index, the International Index of Erectile Function-5 (IIEF-5) scores, age, fasting blood glucose, whole blood count, lipid, and hormone profile values were measured. The PLR and NLR values from both the patient and control groups were evaluated. The IIEF-5 questionnaire was used to measure the quality of erection in all the groups. A total of 131 patients with ED were divided into four groups: severe (34 patients), moderate (32 patients), mild-to-moderate (36 patients), and mild ED (29 patients). In addition, a control group was formed with 26 healthy men. Results: No statistically significant difference was observed between the groups in terms of the mean age, hypertension, smoking status, alcohol use, DM, CAD, and cholesterol and triglycerides levels. The mean PLR values were 125.3 +/- 41.4, 120.6 +/- 36.1, 118.2 +/- 50.4, 104.9 +/- 3.2, and 107.5 +/- 37.4 in the severe, moderate, mild-to-moderate, mild ED, and control groups, respectively. There was no significant difference in the PLR ratio among the ED groups and controls (p>0.05). The mean NLR values were 2.40 +/- 1.22, 2.34 +/- 0.88, 2.26 +/- 1.22, 2.1 +/- 0.87, and 1.76 +/- 0.7 in the severe, moderate, mild-to-moderate, mild ED, and control groups, respectively. Compared with the control group, this value was statistically significant for patients with all ED groups (p<0.05). Conclusion: The NLR value was found to be higher in patients with ED. The NLR value may be related to ED, and it can give valuable information in patients with ED.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2019Visceral adiposity index is useful for evaluating obesity effect on erectile dysfunction
Akdemir, Alp Ozgur; Karabakan, Mehmet; Aktas, Binhan Kagan; Bozkurt, Aliseydi; Ozgur, Emrah Gokay; Akdogan, Nebil; Yarıs, Mehmet
Studies show that erectile dysfunction (ED) is associated with obesity, and it has been shown that the possibility of developing sexual dysfunction in obese men is 30% higher compared to those with normal weight. Obesity is measured using various methods, for example waist circumference (WC) measurement or body mass index (BMI), but recently, visceral adiposity index (VAI) has also been utilised to better assess obesity and metabolic syndrome. In our study, the potential link between VAI and ED was investigated. The data of 176 patients who presented to the urology outpatient clinic with erection complaints were retrospectively screened. A control group was also established with 122 men without complaints of erectile dysfunction. The erectile functions of all participants were determined using the International Erectile Function Index-5 (IIEF-5) scoring. In addition, their serum fasting blood glucose, total testosterone (TT), triglyceride (TG), low-density lipoprotein (LDL) cholesterol and high-density lipoprotein (HDL) cholesterol levels were measured. The physical examination comprised the measurement of WC, height and weight, and BMI. The mean age of the participants was 58.7 ± 8.4 for the ED group and 57.1 ± 7.5 for the control group. The mean VAI was statistically significantly higher in the ED group (5.32 ± 2.77) compared to the control group (4.11 ± 1.93) (p < 0.001). Since VAI contains both physical and metabolic parameters, our findings suggest that it discloses the effects of WC, BMI, HDL and TG more clearly. VAI is considered useful for the assessment of the effect of obesity on ED patients.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2018A novel therapeutics agent: antioxidant effects of hydroxylfasudil on rat kidney and liver tissues in a protamine sulphate-induced cystitis rat model; preliminary results
Bozkurt, Aliseydi; Budak, Harun; Erol, Huseyin Serkan; Can, Serpil; Mercantepe, Tolga; Akin, Yigit; Ozbey, Isa; Cankaya, Murat; Halici, Mesut Bunyamin; Coban, T. Abdulkadir
Cystitis is defined as an inflammation of the bladder caused by a bacterial infection, and it can be dangerous and painful when it spreads through the internal organs. In this study, antioxidant effects of hydroxylfasudil (HF) at the enzymatic and molecular level on kidney and liver tissues in cystitis rat model, which is caused by inflammation of the rat bladder with a protamine sulphate (PS), was examined. Quantitative changes of reduced glutathione (GSH) and lipid peroxidation (LPO) levels, which are a marker for oxidative stress, were determined in rat kidney and liver tissues for each groups. And then molecular and biochemical impact of HF treatment on antioxidant enzymes including superoxide dismutase (SOD) and catalase (CAT) in cystitis model were studied. The results suggest that HF could be beneficial to the renal and hepatic antioxidant system. Thus, HF might be used as a novel therapeutics agent to eliminate interstitial cystitis.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2018Low serum melatonin levels are associated with erectile dysfunction
Bozkurt, Aliseydi; Karabakan, Mehmet; Aktas, Binhan Kagan; Gunay, Murat; Keskin, Ercüment; Hirik, Erkan
Objective: Melatonin is a hormone secreted from the pineal gland and has anti-oxidative and anti-inflammatory effects. Oxidative stress is considered as an important factor in the etiology of erectile dysfunction (ED), and in many experimental models, positive results have been obtained with melatonin treatment. This study aimed to measure serum melatonin levels in ED patients and to investigate the possible relationship between ED and melatonin levels. Materials and Methods: Sixty-two patients diagnosed with mild, moderate or severe ED according to the five-item International Erectile Function Index (IIEF-5) and 22 healthy individuals were included in the study. The serum melatonin levels, anthropometric data, and other biochemical and hormonal parameters of all the subjects were recorded. Detailed anamnesis was also obtained in terms of diabetes, hypertension, cardiovascular diseases, smoking status, and alcohol use. Results: The serum melatonin level was found 34.2±13.3 ng/dL in the mild ED group, 33.3±14.7 ng/dL in the moderate ED group, 34.8±17.2 ng/dL in the severe ED group, and 44.6±16.5 ng/dL in the control group. The serum melatonin levels were significantly lower in all ED groups compared to the control group (p=0.019). There was no significant difference in the serum melatonin levels between the three ED groups. Diabetes, hypertension, cardiovascular diseases, smoking and alcohol use were not significantly different between the ED groups (p>0.05). Conclusion: We consider that if our findings are supported by further studies with larger populations, the measurement of the serum melatonin level may have a future role in the diagnosis and treatment of ED.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2018Does extracorporeal shockwave lithotripsy therapy affect thiol-disulfide homeostasis?
Bozkurt, Aliseydi; Mertoglu, Cuma; Karabakan, Mehmet; Siranli, Gulsah; Yurt, Emine Feyza; Erel, Ozcan
Objective: Extracorporeal Shockwave Lithotripsy (ESWL) is a non-invasive method that is effective at crushing stones in the upper urinary tract. Disturbance of the thiol/disulfide homeostasis, in favor of the disulfide, has been shown to be involved in the disease pathogenesis. Methods: A total of 36 individuals that underwent ESWL had blood samples collected before ESWL (0hrs), 6hrs, and one week after the ESWL. Sera native and total as wells as disulfide amount was measured using an automated method sodium borohydrate (NaBH4) reduction. In addition, Ischemia Modified Albumin (IMA) levels were measured using colorimetric assay method. Results: Native thiol level was reduced at the 6th hour following ESWL compared to baseline. While the ratios of disulfide level, Disulfide/Total Thiol (DTT), Disulfide/Native Thiol (DNT) and IMA level were increased at the 6th hour following ESWL compared to baseline, they were found to be similar with their baseline values at the end of 1st week. Total thiol and native/total thiol did not show any significant change. Conclusions: ESWL treatment disrupts thiol/disulfide homeostasis and the structure of albumin at the acute term. Therefore, it increases protein oxidation and leads to increased oxidative stress. However, this state is transient and returns to normal within the proceeding days.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2018Is there any relationship between serum levels of total bilirubin and the severity of erectile dysfunction?
Keskin, Ercüment; Karabakan, Mehmet; Bozkurt, Aliseydi; Hirik, Erkan; Karabulut, İbrahim; Gunay, Murat; Çakan, Murat
OBJECTIVE: Recent studies have shown that atherosclerosis is associated with erectile dysfunction and the serum bilirubin level. In this study, the serum total bilirubin levels of patients with erectile dysfunction were measured to investigate the relationship between the levels of erectile dysfunction and total bilirubin. METHODS: A total of 94 patients with erectile dysfunction were divided into three groups; severe erectile dysfunction (33 patients), moderate erectile dysfunction (31 patients), and mild erectile dysfunction (30 patients). In addition, a control group was formed with 31 healthy men. The International Index of Erectile Function-5 Questionnaire was used to measure the quality of erection in all the groups. The body mass index was calculated for all the participants. The serum glucose, low-density lipoprotein and high-density lipoprotein, cholesterol, triglyceride, total bilirubin, and total testosterone levels were also determined. RESULTS: No statistically significant difference was observed between the groups in terms of the mean age, hypertension, smoking status, alcohol use, cardiovascular diseases, hepatobiliary disease, diabetes mellitus, and levels of total testosterone, low-density lipoprotein-cholesterol, and triglyceride. However, high-density lipoprotein, body mass index, and total bilirubin were significantly lower compared to the control group (p < 0.001). The serum total bilirubin level was found to be 0.41 ± 0.21 ng/dL in the severe erectile dysfunction, 0.43 ± 0.19 ng/dL in the moderate erectile dysfunction, and 0.48 ± 0.11 ng/dL in the mild erectile dysfunction groups (p < 0.001). CONCLUSION: Considering the significant differences between the erectile dysfunction and control groups in terms of serum total bilirubin levels, a low level of bilirubin may have a role in the etiology of erectile dysfunction.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2018Evaluation of the effect of retrograde intrarenal surgery with myo-inositol oxygenase
Mertoglu, Cuma; Bozkurt, Aliseydi; Keskin, Ercüment; Gunay, Murat
Objective: To investigate the effect of retrograde intra-renal surgery (RIRS) on kidneys using the myo-inositol oxygenase (MIOX) enzyme. MIOX is a renal tubular-specific novel marker for the early diagnosis of acute kidney injury. Methods: A total of twenty seven individuals that had undergone RIRS to treat kidney stones were included in the study. Biochemical tests were performed on serum samples collected immediately before RIRS (hour 0) and at the 6th and 24th hours after the surgery. Results: The creatinine value at hour 6 was lower than the baseline (hour 0) value (p = 0.0305). Cystatin C at hour 6 was lower than the value measured at hour 24 (p = 0.0142). Similarly, MIOX was lower at hour 6 compared to hour 24 (p = 0.0214). MIOX/creatinine at hour 6 was lower than the value calculated at hour 24 (p = 0.0348). The basal values of MIOX and creatinine were found to have a positive correlation (correlation coefficient r = 0.5946, p = 0.0035). Conclusions: Similar to the serum creatinine, the serum MIOX level provides information about kidney functions. RIRS was confirmed to be a safe procedure for the treatment of acute kidney injury with no negative effects on the kidneys.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2018Impact of overactive bladder on retrograde ejaculation
Salvarci, Ahmet; Karabakan, Mehmet; Bozkurt, Aliseydi; Hirik, Erkan; Aktas, Binhan Kagan
Purpose: To evaluate the impact of overactive bladder disorder on patients diagnosed with retrograde ejaculation. Materials and Methods: Retrospective analysis of prospective collected database made. Questionnaires conducted in urology polyclinics in five different centers. Main Outcome Measure(s): International Index of Erectile Function - 5 (IIEF - 5), Overactive Bladder 8 - Question Awareness Tool (OAB - V8), urodynamics, semen analysis. The participants of the study were n = 120 patients. There was retrograde ejaculation (RE) in only n = 47 patients (non / minimal symptomatic patients), n = 73 patients had RE and overactive (OAB) complaints (symptomatic patients) and received anticholinergic treatment (trospium), n = 37 control group patients who only had OAB and received an anticholinergic. Results: While no difference was observed in overactive bladder examination and urodynamic values between the non / minimal symptomatic group and the symptomatic group (p > 0.05), sperm was detected and identified as fructose positive in post - ejaculation urine in the symptomatic group. Thus, it was possible to demonstrate the differences between symptomatic patients and non - symptomatic patients. Consequently, following three - month daily treatment with trospium 30 mg 2 x 1 in the control group and the symptomatic group, it was observed that an evident increase was observed in the sperm count and ejaculate volume in the symptomatic group and that no change was observed in the control group (p < 0.05). Conclusion: This clinical study is the first of its kind in terms of revealing the coexistence of RE with OAB upon performing urodynamics and showing that treatment is possible in selected patients.
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