Sürdürülebilir Kalkınma Amaçları
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- ArticleAçık Erişim2026The potential of Barbarea species in the management of obesity, hyperlipidemia, and diabetes
Badem, Merve; Kanbolat, Şeyda; Kandemir, Ali; Öztürk, Ayşe Aleyna; Topcu, Hayrünnisa
Barbarea species belonging to the Brassicaceae family contain glucosinolates, flavonoids, phenolic acids, saponosides, fatty acids, and other bioactive compounds. These compounds are reported to exhibit antioxidant, antimicrobial, antimutagenic, antiproliferative, and herbicidal effects, as well as beneficial effects on metabolic diseases. In particular, organosulfur and polyphenolic compounds have been reported to exhibit inhibitory effects on enzymes such as cyclooxygenase, lipoxygenase, nitric oxide synthase, pancreatic lipase, lipoprotein lipase, and glucosidase. In this study, the inhibitory activities of methanol extracts, chloroform, ethyl acetate, and remaining aqueous subfractions obtained from the aerial parts of B. auriculata var. paludosa Coode& Cullen and B. plantaginea DC on pancreatic lipase, cholesterol esterase, and α-glucosidase enzymes were investigated for the first time. When the results obtained in the study are evaluated, the ethyl acetate subfraction of B. plantaginea exhibited the strongest pancreatic lipase and cholesterol esterase inhibitory activities, with IC₅₀ values of 111.687 ± 1.291 µg/mL and 10.718 ± 0.308 µg/mL, respectively. For α-glucosidase inhibition, the methanol extract of B. auriculata subsp. paludosa demonstrated the highest activity with an IC₅₀ value of 135.207 ± 0.136 µg/mL. it is seen that the ethyl acetate subfraction of both species has stronger cholesterol esterase inhibitory effects, moderate lipase and glucosidase inhibitory effects.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2026First Report on the Phytochemical and Pharmacological Properties of Hedysarum yilmazunalii
Osma, Etem; Varol, Tuğçe; Şimşek, Samed; Altın, Sevgi; Yılmaz, Mustafa Abdullah; Aydın, Ali; Elveren, Müjgan; Kandemir, Ali
This study presents the phytochemical and biological evaluation of the species Hedysarum yilmazunalii Kandemir, F. Yıldız & H.İ. Turkoğlu. Methanolic extracts from leaves, stems, and roots were characterized by LC-MS/MS, and their antioxidant, enzyme inhibitory, and antiproliferative activities were investigated. The analyses showed organ-specific differences in phenolic composition, with quinic acid as the major compound in leaves together with higher levels of flavonoid glycosides, including astragalin and isoquercitrin. Aerial extracts showed strong enzyme inhibitory activity. Leaf and stem extracts exhibited cholinesterase inhibition, with IC50 values of 5.82 and 6.42 µg/mL for AChE and 5.41 and 5.50 µg/mL for BChE, respectively. The leaf extract showed α-glucosidase inhibitory activity (4.10 µg/mL), comparable to acarbose, and antioxidant activity (DPPH: 6.46 µg/mL), together with the highest phenolic (145.43 mg GAE/g extract) and flavonoid contents (87.20 mg QE/g extract). Antiproliferative assays demonstrated growth inhibition of lung and breast cancer cell lines, with the lowest GI50 values of 1.00 µg/mL for H1650 and 2.34 µg/mL for MDA-MB-231 cells. LDH cytotoxicity remained low (2.25%–12.59%), indicating limited membrane damage in both cancer and normal cells. These findings identify H. yilmazunalii as a source of compounds with antioxidant, neuroprotective, antidiabetic, and anticancer potential.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2025Functional Ecology of Floral Traits and Plant Pollinator Interactions in Two Endemic Scrophularia L. Species from Erzincan, Türkiye
Kandemir, Ali; Kılıç, Engin; Yildiz, Faruk; Türkoğlu, Halil İbrahim; Yildirim, Nalan
Plant–pollinator interactions are fundamental to reproductive success, yet remain understudied in the Turkish ecological context, particularly for endemic species. This study investigates pollinator diversity, behavior, and environmental influences in two rare Scrophularia L. species endemic to Erzincan, Türkiye: S. fatmae and S. erzincanica. Field observations conducted between 2019 and 2020 documented floral visitors, visitation frequency, and the role of floral morphology in guiding pollinator behavior. Climatic variables such as temperature, humidity, and wind were also analyzed in relation to pollinator activity. The high-altitude S. fatmae was visited exclusively by Bombus niveatus, which proved effective even under cold and windy conditions, whereas S. erzincanica attracted a more diverse community, including Halictus quadricinctus, Halictus sp., and Lasioglossum pauxillum, the efficiency of pollination was strongly linked to floral architecture; clustered flowers and staminodes enhanced pollinator guidance and cross-pollination. Statistical comparisons further highlighted differences in body size and foraging strategies between pollinator groups. Environmental conditions were shown to significantly limit pollinator activity, with high humidity and low temperatures reducing visitation. Additionally, unexpected warming events accelerated phenology in S. fatmae, indicating potential risks from climate change. Overall, this research provides novel insights into the functional ecology of endemic Scrophularia species, demonstrating how floral traits, pollinator behavior, and environmental variables interact to shape reproductive strategies. These findings contribute to biodiversity conservation efforts and underscore the importance of long-term monitoring under changing climatic conditions.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2025Chemical Composition of Hydrodistillated Essential Oil of Heracleum antasiaticum MANDEN (L.) and its Antimicrobial Activity Against Food-Borne Microorganisms
Çoban, Furkan; Çetin, Bulent; Özer, Hakan; Toktay, Zehra; Budak, Beyzanur; Angin, Hilal; Kandemir, Ali
The essential oil obtained by hydrodistillation from the aerial parts of Heracleum antasiaticum MANDEN from T & uuml;rkiye was analyzed by GC and GC-MS. Forty-five compounds were characterized, representing 92.7 % of the total components detected, and elemicin (27.8%), apiole (26.2%), n-octanol (4.9%), alpha-zingiberene (3.9%), octyl acetate (3.8%), and (E)-gamma bisabolene (3.1%) were identified as predominant components. In addition, in this study, the essential oil was tested for its antimicrobial activity against 23 different food-borne microorganisms, including 20 bacteria, 1 fungus, and 2 yeast species. The essential oil of Heracleum antasiaticum exhibited considerable inhibitory effects against all bacteria, fungi, and yeast species tested.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2025Investigation of Antioxidant and Enzyme Inhibitory Activities of Echinophora chrysantha: Microwave-Assisted Hydrodistillation and SPME Analysis of its Essential Oils
Şahin, Osman; Kanbolat, Şeyda; Çolak, Nevin Ulaş; Badem, Merve; Subaş, Tuğba; Şener, Sıla Özlem; Korkmaz, Nuriye; Kalaycıoğlu, Atila Taner; Öztürk, Ergün; Sayım, Alperen Ümit
Echinophora genus is represented by six species in Türkiye, including three endemics. The objective of this study was to ascertain the antioxidant, tyrosinase, and collagenase inhibitory activities and to analyse the volatile oil and extracts derived from E. chrysantha using GC/MS/SPME. The antioxidant and enzyme inhibition activities were evaluated using spectrophotometric methods. The findings indicated that the methanol extract of E. chrysantha exhibited the highest antioxidant activity. Furthermore, both the methanol extract and volatile oil of the plant demonstrated potent inhibitory effects on tyrosinase and collagenase. A total of 84 volatile compounds were identified from the hydrodistilled oil obtained using a microwave oven, and 23 compounds were detected by the solid-phase microextraction (SPME) method. The most frequently occurring volatile components were α-Phellandrene, o-Cymene, p-Cymene, E-β-Farnesene, Carotol, trans-Caryophyllene, and β-Phellandrene. The results of this study indicate that E. chrysantha has the potential to be developed for the treatment of hyperpigmentation issues and anti-aging applications. Furthermore, it may also have applications in pharmacology, cosmetics and the food industry.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2025Comprehensive Analysis of Hedysarum nitidum: Identification Phytochemical Contents, Antioxidant Potential, Antimicrobial Properties, Cytotoxic Effects, and Enzyme Inhibition Activities
Baltürk, Nuray; Badem, Merve; Kanbolat, Seyda; Yıldırım, Sercan; Şen, Tuğba Mazlum; Bozdal, Gözde; Yalçın, Can Özgür; Kandemir, Ali; Korkmaz, Nuriye; Karaoglu, Sengul Alpay
Hedysarum species play a crucial agronomic role due to their high feed quality and ability to enhance soil fertility through nitrogen fixation. Traditionally, they have been used in medicine to boost energy, support the immune system, and benefit the peripheral nervous system. However, the biological activity and phytochemical composition of Hedysarum nitidum remain unexplored. This study aimed to evaluate the bioactivity of methanol (HNM) and water (HNW) extracts from the aerial parts of H. nitidum. Antioxidant (total polyphenol and flavonoid contents, 2,2-diphenyl-2-picrylhydrazyl hydrate [DPPH], ferric-reducing antioxidant power [FRAP], Cu (II) ion reducing antioxidant capacity [CUPRAC]), antimicrobial, cytotoxic, and enzyme inhibitory (tyrosinase, cholinesterase, α-glucosidase, α-amylase) activities were assessed. Phytochemical and essential oil compositions were analyzed using high-performance liquid chromatography (HPLC) and gas chromatography–mass spectroscophy (GC–MS). HPLC identified p-hydroxybenzoic acid, caffeic acid, and 2-hydroxycinnamic acid, whereas GC–MS detected n-octanal, hexahydrofarnesyl acetone, humulene epoxide-II, α-terpineol, and eicosane. HNW and HNM exhibited strong antioxidant activity and enzyme inhibition, suggesting potential applications in hyperpigmentation, diabetes, and Alzheimer's treatment. HNM showed selective antibacterial activity against Gram-positive bacteria, whereas neither extract exhibited cytotoxicity against A549 cells. These findings highlight H. nitidum as a promising candidate for therapeutic applications.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2025Molecular Characterization and Genotypes Structure Analysis of Anatolian Endemic Hesperis isatidea (Brassicaceae) Using of Inter-Simple Sequence Repeat (ISSR) Markers
Yıldız, Faruk; Sevindik, Emre; Özkara, Zekiye; Aydoğan, Melike; Atasagun, Bayram; Çelik, Civan; Kandemir, Ali
Abstract: This research evaluates the molecular characterization and genotype structure analysis of endemic Hesperis isatidea populations using the Inter-Simple Sequence Repeat Polymerase Chain Reaction (ISSR-PCR) technique which were collected from various regions of Erzincan Province, Türkiye, and subsequently processed for genomic DNA isolation and ISSR-PCR analysis. Seven primers were used in the ISSR-PCR analyses and a total of 100 bands were obtained. Of these, 98 were polymorphic and two were monomorphic, and the polymorphism rate was determined to be 98%. The highest number of bands from the primers was obtained from UBC-855 and UBC-826, and the lowest number was obtained from UBC-836. The mean polymorphism information content (PIC) was determined to be 0.2886. The mean gene diversity value was determined to be 0.3559. The UPGMA dendrogram generated by PAUP 4.0b10 consists of two major clades. The genetic distance matrix values of samples from 5 different locations of Erzincan province, produced with the PAUP 4.0b10 program, were determined as 0.18000 and 0.470000, respectively. The PCA analysis created with the program PAST 4.06b consisted of four groups. The PCA analysis and the UPGMA dendrogram were compatible. These findings confirm that ISSR analysis is a highly polymorphic DNA-based marker system and an effective tool for differentiating between genotypes.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2024Erzincan İli-Ergan Dağı’nın Farklı Rakımlarında Yayılış Gösteren Bazı Taksonların Antioksidan Kapasiteleri
Kandemir, Ali; Osma, Etem; Varol, Tuğçe; Şimşek, Samed; Adıgüzelli, Gökhan
Bu çalışmada, Erzincan il sınırları içerisinde bulunan Ergan Dağı’nın farklı rakımlarında 1500 m (Glaucium leiocarpum Boiss., Astragalus ornithopodioides Lam., Alcea calvertii (Boiss) Boiss.), 2000 m (Globularia trichosantha Fisch. & C.A. Mey, Hypericum scabrum L., Tanacetum mucroniferum Hub. Mor. Et Grierson, Inula oculus-christi L., Onobrychis cornuta (L.) Desv., Barbarea auriculata Hausskn. Ex Bornm), 3000 m (Scorzonera sericea DC., Hedysarum erythroleucum Boiss., Astragalus nezaketiae A. Duran & Aytaç) yayılış gösteren 12 bitki türü seçilmiştir. Çalışma ile rakım faktörünün bitkiler üzerindeki etkileri belirlenmesi amaçlanmıştır. Bunun için bitkilerin yaprak, dal ve kök olmak üzere farklı organları etilasetat, metanol ve suda ekstaksiyonu yapılarak fenolik (TPC) ve flavonoid (TPF) içeriği ile antioksidan aktivite (DPPH, FRAP) miktarının analizi yapılmıştır. Antioksidan aktiviteleri incelendiğinde, Globularia trichosantha bitki türünde en yüksek değerler belirlenmiştir. DPPH aktivitesi yaprakta 47,86±1,70, dalda 108,09±1,29, kökte ise 60,57±0,74 olarak ölçülmüştür. Fenolik içerik ise yaprakta 82,56±0,46, dalda 56,71±2,12, kökte ise 59,75±2,11 olarak tespit edilmiştir. FRAP aktivitesi yaprakta 54,87±1,54, dalda 41,09±3,06, kökte ise 48,95±2,73 olarak belirlenmiştir. Flavonoid içerik verileri ise Globularia trichosantha yaprakta 59,81±2,31, dalda 31,62±3,55 olarak tespit edilirken, Hypericum scabrum kökte 35,71±2,73 olarak belirlenmiştir. Analiz sonuçları SPSS 22 Paket İstatistik Programında değerlendirilmiş olup rakımlar (yükselti) arasında farklılıklar tespit edilmiştir. Bitkilerin fenolik ve flavonoidlerin içerikleri ile antioksidan aktiviteleri arasında özellikle metanolde çözünen örneklerde güçlü pozitif yönde korelasyon bulunmuştur. Sonuç olarak, bu çalışma farklı rakımlarda yetişen bitkilerin fenolik ve flavonoid içeriği ile antioksidan aktivitelerindeki değişkenliği açıklamaktadır. Bulgular, rakımın bu bitki türlerinin biyoaktif bileşenleri ve antioksidan potansiyeli üzerindeki etkisini vurgulamaktadır. Ayrıca, 2000 m rakımda yetişen Hypericum scabrum L., Tanacetum mucroniferum Hub. Mor. Et Grierson, Globularia trichosantha Fisch.&C.A.Mey bitkilerinin gösterdiği antioksidan performansı, bu bitkilerin farmasötik veya diğer uygulamalarda daha fazla keşif için potansiyel oluşturmaktadır.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Erzincan İli-Ergan Dağı'nda Yaygın Bitki Türleri ve Topraklarındaki Mineral Elementlerin Rakımsal Değişimi
Elverici, Mert; Kandemir, Ali; Kılıç, Engin; Osma, Etem; Varol, Tuğçe; Yildiz, Faruk; Türkoğlu, Halil İbrahim; Hasbenli, Abdullah; Kiymaz, Muhammet Onur
Bu çalışma, 2021-2022 yıllarında Erzincan il sınırları içerisinde yer alan Ergan Dağı’nın farklı yüksekliklerinde yetişen 30 bitki türü ile yapılmıştır. Bunun için 1500 m yükseklikte (Glaucium leiocarpum Boiss., Alcea calvertii, Genista aucheri, Astragalus ornithopodioides, Anchusa leptophylla, Linum mucronatum, Euphorbia virgata) 2000 m’de (Genista aucheri, Hypericum scabrum, Tanacetum mucroniferum, Barbarea auriculata, Onobrychis cornuta, Inula oculus-christi, Euphorbia virgata, Globularia trichosantha) 2500 m’de (Aster alpinus, Lallemantia canescens, Scutellaria orientalis, Dianthus orientalis, Coronilla orientalis, Campanula tridentata, Crepis armena, Anthemis cretica, Euphorbia petrophila, Papaver fugax) ve 3000 m’de (Senecio hypoleucus, Pedicularis comosa, Scorzonera sericea, Anthemis cretica, Astragalus nezaketiae, Papaver fugax, Campanula tridentata, Senecio hypoleucus, Hedysarum erythroleucum) olacak şekilde farklı rakımlardan toplanan bitkiler ve yetiştikleri topraklarda mineral elementlerin konsantrasyonu belirlenmiştir. Toplanan bitki (kök, yaprak, çiçek) ve toprak örnekleri ön işlemlerden geçirildikten sonra ICP-MS cihazında analiz edilmiştir. Elde edilen veriler istatistiksel olarak değerlendirilerek, rakımlar arasındaki farklılıklar olduğu tespit edilmiştir. Yükselti arttıkça topraktaki elementlerin genel olarak azaldığı görülmüştür.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Analysis of volatile compounds of alcea calvertii boiss. and its antimicrobial, anticholinesterase, and antityrosinase potency
Sener, Sila Ozlem; Karaoğlu, Şengül Alpay; Aliyazicioglu, Rezzan; Çolak, Nevin Ulaş; Kandemir, Ali; Özgen, Ufuk; Badem, Merve; Kanbolat, Şeyda
The research aimed to analyze the volatile compounds by using SPME-GC-MS and to detect anticholinesterase, antityrosinase, and antimicrobial potential of metanol extract of Alceacalvertii Boiss., an endemic species for Türkiye. The inhibitory effects of tyrosinase, acetylcholinesterase, and butyrylcholinesterase of the plant were determined by spectroscopic technique and the plant’s antimicrobial activity was assessed using the agar diffusion method. A total of 18 volatile compounds were specified belonging to terpenes classes. o-cymene (10.60%) and sesquicineole (15.55%) were detected as major volatile components of the species by the SPME-GC-MS technique. Meaningful antimicrobial activity was observed on Candida tropicalis, Enterococcus faecalis, Escherichia coli, Mycobacterium smegmatis, Staphylococcus aureus, and Pseudomonas aeruginosa. The half maximal inhibitory concentration (IC50) value of the plant was determined 60.12 ± 1.75 μg/mL as a result of tyrosinase assay. IC50 value was found 111.54 ± 1.75 μg/mL, according to butyrylcholinesterase inhibition studies. Considering all the findings, it has been determined that the plant includes diverse volatile compounds and showed promising antimicrobial, tyrosinase inhibitory, and moderate butyrylcholinesterase inhibitory effects so Alceacalvertii may be the up-and-coming source of natural medicine for microbial and dermatological diseases although limited to its effects for Alzheimer's disease.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2024Phytochemical analysis and biological evaluation of Ferulago setifolia K. Koch
Gözcü, Sefa; Akşit, Zeynep; Şimşek, Samed; Kandemir, Ali; Aydın, Ali; Yılmaz, Mustafa Abdullah; Akşit, Hüseyin
BACKGROUND: Ferulago setifolia K. Koch (Apiaceae) has been the subject of this study, aiming to comprehensively determine its phenolic fingerprint and evaluate its various biological activities. The liquid chromatography–tandem mass spectrometry analysis of the 70% methanol extract of F. setifolia (FS) revealed the presence of 23 phytochemicals, among which chlorogenic acid, quinic acid, kaempferol-3-O-glucoside, and quercetin-3-O-glucoside were identified as the major phenolics in the extract. RESULTS: The biological screening included examinations of antioxidant, antibacterial, antiproliferative, and cytotoxic activities. The FS extract displayed moderate 2,2-diphenyl-1-picrylhydrazyl radical scavenging and ferric-reducing capacity, indicating moderate antioxidant activity. Furthermore, FS exhibited significant antiproliferative effects on cancer cells while showing low cytotoxicity on normal cells. The antibacterial activity findings revealed that FS demonstrated potent activity against Pseudomonas aeruginosa, Bacillus cereus, Staphylococcus aureus, and Escherichia coli. CONCLUSION: The findings of this study suggest that the methanolic extract of FS holds promise as a potential source of biologically active compounds. It can be utilized for the development of pharmaceutical formulations, thanks to its significant antiproliferative and antibacterial activities. Additionally, FS can serve as a valuable source of chlorogenic acid for industrial applications.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2024Phytochemical profile by LC-MS/MS analysis and evaluation of antioxidant, antidiabetic, anti-Alzheimer, and anticancer activity of Onobrychis argyrea leaf extracts
Yeniçeri, Esma; Altay, Ahmet; Koksal, Ekrem; Altın, Sevgi; Taslimi, Parham; Yılmaz, Mustafa Abdullah; Cakir, Oğuz; Tarhan, Abbas; Kandemir, Ali
Introduction: Studies on different species of Onobrychis, a member of the Fabaceae family, have revealed a number of biological activities with potential applications in alternative medicine. The phytochemical content of the plant Onobrychis argyrea subsp. argyrea and some of the important biological activities linked to its metabolism are quite limited. Therefore, this study was the first to determine the content of secondary metabolites in this species and to elucidate important biological activities related to metabolism. Methods: LC-MS/MS was used for the quantitative analysis of bioactive compounds in the aerial part of O. argyrea extracts. The antioxidant properties of the extracts were assessed based on their ability to scavenge DPPH radicals and reduce iron ions. The anti-diabetic effect of the extracts was tested by the inhibition of α-amylase and α-glycosidase enzymes, and the anti-Alzheimer's ability was tested by the inhibition of the enzymes AChE and BChE. The cytotoxic effects of the extracts were tested on four different cancer lines, namely A-549, HT-29, MCF-7 and MDA-MB 453 using the XTT assay. The mechanism of the anti-cancer effect was determined by means of flow cytometry analysis on HT-29 cells. Results: Twenty-nine phytochemicals were identified via the LC-MS/MS analysis in the plant extracts. The most abundant phytochemicals in the extracts were quinic acid, isoquercitrin, epicatechin, and routine, respectively. Antioxidant analysis showed that among all extracts, methanol extract (ME) was most effective in scavenging DPPH (IC50: 23.93 ± 0.96 µg/mL) and in reducing iron ions (53.24 ± 2.14 mgTEAC/g). Enzyme activity studies demonstrated that ME is a potent inhibitor of α-glycosidase (IC50: 15.06±0.64 µg/mL), AChE (IC50: 24.75±0.76 µg/mL), and BChE (IC50: 3.36±0.14 µg/mL). XTT assay results disclosed the strongest anti-proliferative activity of ME against the human colorectal adenocarcinoma HT-29 cell line with an IC50 value of 33.35±0.92 µg/mL. Flow cytometry analysis revealed that ME caused mitochondrial membrane damage in HT-29 cells (**p<0.01) and subsequently induced apoptosis (**p<0.01) by activating the caspase pathway (**p<0.001). Conclusion: O. argyrea, rich in bioactive compounds, showed remarkable antioxidant, antidiabetic, Alzheimer's, and anticancer properties in vitro, demonstrating that this plant contains secondary metabolites that may be potential drug candidates for the treatment of metabolic diseases.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024LC-MS /MS characterization and biological activities of Morina persica L. (Caprifoliaceae)
Gözcü, Sefa; Akşit, Zeynep; Şimşek, Samed; Kandemir, Ali; Aydin, Ali; Yilmaz, Mustafa Abdullah; Akşit, Hüseyin
The study aimed to comprehensively identify the phenolic fingerprint of Morina persica L. (Caprifoliaceae) and evaluate its various biological activities. LC-MS/MS analysis of 70% Morina persica methanol extract revealed the presence of 27 phytochemicals, with quinic acid, chlorogenic acid, and rutin being the major phenolics. The antioxidant, antibacterial, antiproliferative, and cytotoxic activities were evaluated for biological screening. The results showed that the methanolic extract of M. persica has a moderate DPPH radical scavenging and ferric-reducing capacity, indicating antioxidant activity. M. persica was observed to have a sufficient antiproliferative effect against cancer cells and low cytotoxicity against normal cells. Moreover, M. persica demonstrated good antibacterial activity against Clostridium perfringens, Enterococcus faecalis, and Escherichia coli. These data suggest that the methanolic extract of M. persica could be considered both an industrial source of quinic acid and a potential biologically active ingredient for developing drug formulations.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2024Preserving the richness of nature: cultural and ecological importance of edible wild plants in Sivas
Kadioglu, Zakine; Yildiz, Faruk; Kandemir, Ali; Cukadar, Kemal; Kalkan, Nalan Nazan; Vurgun, Huseyin; Donderalp, Veysel; Korkut, Recep; Kaya, Ozkan
This comprehensive article offers a thorough exploration of the abundant plant species that hold a deep connection with the history and daily life of Sivas, within the context of its traditional culture and ethnobotany. The Asteraceae family, which encompassed 22 species, emerged as the most diverse family. Other notable families included Brassicaceae (8 species), Fabaceae (9 species), Polygonaceae (10 species), Apiaceae (11 species), and Lamiaceae (21 species), and the study systematically classified 128 plant species and subplants into 29 different families. The study categorized these plants based on their edible parts to understand their role in Sivas’ culinary traditions, revealing a wide range of edible components, including leaves, seeds, flowers, roots, tubers, gum, fruits, branches, and more. Cerinthe minor, Berberis vulgaris, Stachys lavandulifolia and others provided nourishing leaves and young shoots. The study categorized plants based on their preparation methods, highlighting their integral role in Sivas’ traditional cuisine. Seeds namely Echinops orientalis and Cephalaria procera introduced culinary diversity, while certain plants, such as Geranium tuberosum and Cirsium rhizocephalum, contributed edible roots, expanding the repertoire of local dishes. Whether utilized in cooked dishes, salads, or as spices, these plants impart unique flavors and aromas to the local culinary creations. Whether used in cooked dishes, salads, or as spices, these plants add unique flavors and aromas to local food. Spices such as Mentha longifolia and Thymus leucotrichus enriched the culinary landscape, while Thymus sipyleus and Ziziphora clinopodioides brought their distinct tastes to dishes. The repeated use of common names across different species, such as “Yemlik” “Yağlıca” “Nane” and “Kekik” suggests a shared cultural heritage and linguistic connection among these plants, further emphasizing their significance in the local context. Five species have been newly documented as additions to the flora of Sivas. In summary, this study underscores the importance of preserving and appreciating the botanical knowledge and practices of Sivas traditional culture and ethnobotany. The numerical values within the findings quantitatively showcase the ecological and cultural richness of these plant species, thus making this subject a vital area of study for researchers and a valuable resource for conservationists aiming to safeguard this unique cultural and botanical heritage.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2024Comparison of the phenological, ecological, and vegetative characteristics of two rare endemic species, Scrophularia fatmae and Scrophularia erzincanica adapted to different altitudes specific to Erzincan/ Türkiye
Yıldız, Faruk; Kandemir, Ali; Kılıç, Engin; Türkoğlu, Halil İbrahim; Doğan, Nalan Yıldırım
The ecological interactions between plants and pollinators significantly contribute to global diversity. Hence, focusing on the phenology of plants at various altitudes yields valuable insights into understanding how climate change impacts ecosystems and its association with evolutionary processes. Specifically, this study examines Scrophularia erzincanica, a species native to Erzincan (Türkiye), adapted to lower altitudes, and S. fatmae, an obligate alpine species. The aim was to elucidate the phenological, ecological, and floral characteristics relevant to pollination in both species comparatively. Significant differences were observed in the duration of phenological events, inflorescence architecture, style, and staminode position. Notably, S. fatmae exhibited longer durations for phenological events. Additionally, distinct floral arrangements were evident S. fatmae displayed clustered flowers while S. erzincanica exhibited loosely distributed ones. In S. erzincanica, the staminode's placement in the middle of the corolla created a physical barrier, potentially preventing nectar theft, influencing pollinator selection, prolonging their visit duration, and coordinating flower movements triggered by pollinators. Conversely, in S. fatmae, the staminode remained concealed and positioned near the corolla base. Pollinator visits were shorter in S. fatmae but longer in S. erzincanica. The harsher environmental conditions and lower pollinator density and diversity in alpine regions compared to lower altitudes likely contribute to these differences. Despite sharing common ancestors, these observed phenological disparities in species might stem from selective pressures exerted by varying altitudes, driving these species along divergent evolutionary paths.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023Doğu Anadolu Bölgesinde (Türkiye) Yayılış Gösteren Scrophularia erzincanica ve Scrophularia fatmae (Scrophulariaceae) Popülasyonlarının Genetik Çeşitlilik Analizleri
Kandemir, Ali; Kılıç, Engin; Yildiz, Faruk; Türkoğlu, Halil İbrahim; Yildirim, Nalan
Scrophularia fatmae Kandemir & İlhan ve Scrophularia erzincanica R.R. Mill (Scrophulariaceae) Erzincan/Türkiye'ye özgü endemik türlerdir. S. erzincanica ve S. fatmae'de ki IUCN kategorileri sırasıyla EN ve CR'dir. Bu çalışmada, ISSR belirteçlerini kullanarak Erzincan'daki üç S. erzincanica popülasyonunun ve iki S. fatmae popülasyonunun genetik varyasyon seviyelerini ve genetik yapısı araştırılmıştır. Bu amaçla 10 primer, 75 bireyden oluşan beş popülasyondan 104'ü (%89.6) polimorfik olmak üzere toplam 116 bandı amplifiye edildi. UPGMA küme analizi, genetik varyasyonlar ve coğrafi mesafeler arasında anlamlı bir ilişki olduğunu göstermiştir. Alpin bölgesine uyum sağlamış olan S. fatmae'nin yayılış alanı ve populasyon büyüklüğü daha küçüktür. Ek olarak, S. fatmae'nin kendi kendine tozlaşma eğilimi daha yüksektir. S. erzincanica daha büyük bir popülasyona sahiptir. S. erzincanica ve S. fatmae'de etkili tozlayıcı sayısı sırasıyla üç ve birdir. Bu sebeplerin de katkısı ve araştırma sonuçlarına göre S. fatmae ve S. erzincanica türleri karşılaştırıldığında S. erzincanica'da genetik çeşitliliğin daha fazla olduğu görülmektedir. Bu çalışma sonucunda elde edilen genetik veriler, S. erzincanica ve S. fatmae türlerinin yanı sıra diğer nadir ve tehlike altındaki bitki türleri için koruma stratejilerinin geliştirilmesinde kullanılabilir.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023Ergan Dağı’nın Farklı Yükseltilerinde Yaygın Olarak Yetişen Bitkilerde Ağır Metal (Ba, Cd, Co, Cr, Pb) Birikiminin Belirlenmesi
Elverici, Mert; Kandemir, Ali; Kılıç, Engin; Osma, Etem; Varol, Tuğçe; Yildiz, Faruk; Türkoğlu, Halil İbrahim; Hasbenli, Abdullah; Kiymaz, Muhammet Onur; Akverdi, Aysel
Bu çalışma, Erzincan il sınırları içerisinde yer alan Ergan Dağı’nın farklı yüksekliklerinde yetişen yaygın 30 bitki türü ile yapılmıştır. Çalışma kapsamında 1500 m, 2000 m, 2500 m ve 3000 m olmak üzere farklı yüksekliklerden toplanan bitkilerde ve yetiştikleri topraklarda metal (Cd, Cr, Pb, Co, Ba) konsantrasyonları tespit edilmiştir. Toplanan bitkilerin çiçek, yaprak ve kök ile yetiştikleri topraklar laboratuvarda ön işlemlerden geçirilmiştir. Hazır hale getirilen örneklerde metal konsantrasyonları ICP-MS’te analizi gerçekleştirilmiştir. Elde edilen bitki ve toprağa ait metal verileri istatistiksel olarak değerlendirilerek, her bir metal için rakımlar arasındaki farklılıklar belirlenmiştir. Çalışmada Cd ve Pb konsantrasyonunun 2500 m yetişen bitkilerde ve topraklarda yüksek olduğu belirlenmiştir. İlave olarak, Turanecio hypochionaeus ve Crepis armena türlerinin bünyesinde kadmiyum metalini toprağa göre daha fazla biriktirdiği ve bazı türlerin biyomonitor özelliğe sahip olduğu anlaşılmıştır.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023CHEMICAL COMPOSITION AND ANTIFUNGAL ACTIVITY OF Origanum acutidens ESSENTIAL OIL AGAINST Sclerotinia sclerotiorum (Lib.) DE BARY AND Phytophthora infestans (Mont.) DE BARY
Bayar, Yusuf; Şimşek, Samed; Akşit, Zeynep; Kandemir, Ali
The aim of this study was to investigate the chemical components and evaluate the antifungal activity of Origanum acutidens essential oil. The aerial parts of O. acutidens were collected, and the hydrodistillation method was used to extract the essential oil. Gas chromatography-mass spectrometry (GC-MS) analysis was performed to determine the chemical composition of the essential oil. The main components identified were α-terpineol (4.76%), p-cymene (7.6%), linalool (14.82%), and carvacrol (49.4%). The essential oils were tested against two pathogens in vitro experiments to evaluate their antifungal activity. Different concentrations of the essential oil were applied, and the inhibition of mycelial growth was measured. The results demonstrated that the essential oil exhibited antifungal properties against both pathogens. At a dose of 4.8 µL/Petri dish, the mycelial growth of both pathogens was completely inhibited. However, Sclerotinia sclerotiorum showed higher tolerance to the essential oil compared to Phytophthora infestans. Furthermore, a dose-effect study was conducted as a part of this research. The LC50 values (lethal concentration at which 50% of the pathogens' growth is inhibited) for P. infestans and S. sclerotiorum were calculated as 0.982 µL/Petri and 1.61 µL/Petri, respectively. The study concluded that the essential oil of O. acutidens has the potential to be a natural antifungal agent, particularly against S. sclerotiorum and P. infestans. However, further research is needed to investigate the mechanisms of action and explore potential applications of this essential oil in managing plant diseases.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023α-AMYLASE, α-GLUCOSIDASE AND LIPASE INHIBITORY PROPERTIES AND PHYTOCHEMICAL ANALYSIS OF ENDEMIC PLANT Jurinea brevicaulis Boiss
Sener, Sila Ozlem; Kanbolat, Seyda; Ulas Colak, Nevin; Badem, Merve; Aliyazicioglu, Rezzan; Ozgen, Ufuk; Kandemir, Ali
Obesity, defined as New World Syndrome, causes global health problems and big economic losses. Natural products have gained increasing importance because of their antiobesity potency. The genus Jurinea Cass. with approximately 200 described species worldwide has been traditionally used as a therapeutic agent for colic, fever, gout and rheumatism. The aim of this study was to analyze the volatile components, to determine phenolic compounds and to evaluate α-amylase, α-glucosidase, and lipase inhibitory activities of the endemic plant species Jurinea brevicaulis Boiss. The widely used solid-phase microextraction technique (SPME) was employed for Gas Chromatography-Mass Spectrometry (GC/MS) analysis of the volatile components. Quantitative analysis of phenolic compounds was performed using Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC). A total of 19 volatile components were specified and o-cymene (10.60 %), β-bisabolene (9.30 %), and sesquicineole (57.5 %) for different terpenes were described as major components. According to the RP-HPLC analysis, sinapic acid, p-coumaric acid and quercetin were determined for the species. IC50 values of the species were determined as 36.59 ± 2.37 μg/mL and 42.56 ± 2.83 μg/mL for α-amylase and α-glucosidase assays, respectively. IC50 value was found as 50.31 ± 3.75 μg/mL with the lipase inhibition analysis. In conclusion, it has been determined that J. brevicaulis included diverse volatile components, three phenolic compounds with antiobesity effect potential, which highlights J. brevicaulis as the up-and-coming candidate of natural product source to be used against obesity.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023Seeds or Parasites? Clinical and Histopathological Features of Seeds and Parasites in the Appendix
Issin, Gizem; Demir, Fatih; Simsek, Hasan Aktug; Cagatay, Diren Vuslat; Tayfur, Mahir; Kandemir, Ali; Balci, Mecdi Gürhan
Objective: Parasites and plant seeds may both be found in appendectomy specimens. Each plant seed has a different appearance and can thus exhibit wide variations under the microscope. Fragmented seeds may histologically mimic parasites. The differential diagnosis between seeds and parasites can be challenging in such cases. This study aimed to determine the incidence of parasites, seeds, and foreign bodies in appendectomy materials and highlight the most characteristic histopathological features associated with these structures. Material and Method: In this study, pathology slides of 9,480 patients, who underwent appendectomy between 2010 and 2021, were reviewed, and cases that contained parasites, seeds, or foreign bodies were identified. We reviewed the literature on seeds and parasites in appendectomy specimens. Results: Parasites were observed in 56 (0.6%) cases. Of these cases, 45 had Enterobius vermicularis (80%), and 11 had Taenia subspecies (20%). Plant seeds were observed in 47 cases (0.5%), and were macroscopically recognizable in 5 of them as olive, lemon, and cherry seeds. Parasites and seeds were usually observed in the lumen of appendix vermiformis, filled with abundant fecal materials. Conclusion: Seeds are seen in approximately 0.5% of the appendectomy specimens. Though rarely seen, the fragmented seed appearance may cause diagnostic difficulties. In this context, the key morphological features of parasites and plant seeds outlined in this study may be helpful in their differential diagnosis.
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