Sürdürülebilir Kalkınma Amaçları
Yayınlar
- ArticleAçık Erişim2020Nem ve Hava Koşullarının Subklinik I nflamasyon ve Oksidatif Stres Markırları Ü zerine Etikileri
Mertoglu, Cuma; Gül, Vahdet; Gunay, Murat
Romatoid artritli hastaların semptomlarının soğuk, nemli ve yağmurlu günlerde arttığı bilinmekle birlikte, mekanizması açık değildir. Bu çalışmada, farklı hava koşullarında, bazı inflamatuar ve oksidatif stres markırlarının üzerine etkilerinin araştırılması hedeflenmiştir. Bazı inflamatuar, oksidan ve antioksidan markırların düzeyleri, güneşli, bulutlu ve yağmurlu günlerde sağlıklı ratların serumunda ölçüldü. Glutatyon redüktaz (GR) ve indirgenmiş glutatyon hem bulutlu hem de yağmurlu günlerde, güneşli güne kıyasla daha düşük olarak bulundu (p = 0.001 ve 0.004 sırasıyla). α – glutatyon-S-transferaz yağmurlu günde güneşli güne kıyasla daha düşük olduğu bulundu (p = 0.017). Malondialdehit (MDA) yağmurlu günde güneşli güne kıyasla daha yüksek olarak bulunmuştur (p = 0.044). Nitrik oksit ve interlökin-1 β (IL-1β) bulutlu ve yağmurlu günde güneşli güne kıyasla daha yüksek bulunmuştur (p = 0.003 ve 0.025, sırasıyla). Bulgular, yağmurlu ve bulutlu günlerde oksidatif stres ilişkili inflamasyonun tetiklendiğini göstermektedir.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2020Is ischemia associated with the formation of White matter lesions in migraine?
Ersoy, Alevtina; Yasar, Hasan; Mertoglu, Cuma; Koc, Ural; Akturan, Selcuk; Gok, Gamze; Erel, Ozcan
Objective: White matter lesions (WMLs) are more common in migraine patients than in the normal population. Ischemia/hypoxia and oxidative stress are considered to play a role in WMLs formation. This study aimed to investigate ischemia-modified albumin (IMA), ferroxidase and thiol/disulfide homeostasis in migraineurs with and without WMLs. Patients and Methods: Sixty-two migraineurs with WML, 59 migraineurs without WML and 61 controls were included in the study. All participants underwent brain MRI. WMLs was evaluated according to the Fazekas scale. IMA, ferroxidase, total thiol, native thiol and disulfide measurements were carried out in all participants. Results: The IMA levels were higher in the migraine groups compared to the control group (p < 0.001) and in the WML group compared to non-WML (p < 0.001). The total and native thiol levels were higher in the non-WML group compared to the control and WML groups (p < 0.001 for both). The disulfide levels were similar between the control and non-WML groups, but they were significantly lower in the WML group compared to the control and non-WML groups. There was no significant difference between the groups in terms of the ferroxidase levels (p = 0.092). The thiol/disulfide, IMA and ferroxidase levels were not significantly correlated with the frequency and duration of attacks, severity of pain and disability due to migraine. Conclusion: Increased serum IMA levels in migraineurs point to the role of ischemia/hypoxia, and increased total thiol and decreased disulfide levels indicate an oxidant/antioxidant imbalance in migraine. Ischemia/hypoxia may play a role in WMLs formation in migraine.
RDL Kalıcı Kaydıİncele - CorrectionYalnız künye2020Corrigendum to “Is ischemia associated with the formation of white matter lesions in migraine?” [Clin. Neurol. Neurosurg. 193 (2020) 105770] (Clinical Neurology and Neurosurgery (2020) 193, (S030384672030113X), (10.1016/j.clineuro.2020.105770))
Ersoy, Alevtina; Mertoglu, Cuma; Yasar, Hasan; Koc, Ural; Akturan, Selcuk; Gok, Gamze; Erel, Ozcan
The authors regret that the second and the third name in the order of the authors were erroneously misplaced in the published article. As indicated in the “Authors’ individual contributions,” Cuma Mertoglu participated in Data Curation, Validation, Writing – Review and Editing, while Hasan Yasar contributed to Investigation only. Since the role of Cuma Mertoglu in writing the article is more significant than that of Hasan Yasar we have made changes to the order of authors. The authors would like to apologise for any inconvenience caused.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2020Serum SCUBE-1 levels in patients with diabetic retinopathy
Icel, Erel; Icel, Aykut; Mertoglu, Cuma; Tasli, Nurdan Gamze; Karakurt, Yucel; Ucak, Turgay; Gunay, Murat
Purpose: Signal peptide-CUB-Epidermal growth factor-like domain-containing protein 1 (SCUBE-1) is a protein expressed on the cell surface of endothelial cells and platelets. We aimed to determine the alterations in SCUBE-1 levels in patients at different stages of diabetic retinopathy (DR). Materials and methods: Participants were grouped into 4 regarding the presence and stage of diabetic retinopathy as follows: control group (n 32), patients without DR (n 39), patients with background DR (n 42) and patients with proliferative DR (n 55). Age, gender, duration of diabetes, fasting blood glucose levels, serum hemoglobin A1c (HbA1c), SCUBE-1 and malondialdehyde (MDA) levels of the patients were recorded. Central macular thickness and central macular volume were recorded and compared between groups. Results: Totally 136 patients with diabetes mellitus and 32 control cases were included in the study. There is a significant increase in SCUBE-1 and MDA levels with an advance in DR. In correlation analysis, there was only a significant correlation present between serum SCUBE-1 and MDA levels (p 0.001) but not with other parameters. Conclusion: For the first time in literature, we determined a significant increase in SCUBE-1 levels in patients with DR. Moreover, with an advance in DR, SCUBE-1 levels were also increasing.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2019A Study of Inflammatory Markers in Gestational Diabetes Mellitus
Kulhan, Mehmet; Mertoglu, Cuma; Günay, Murat; Kulhan, Nur Gözde; Güngör, Mehmet
OBJECTIVE: Gestational diabetes mellitus is a common disorder of carbohydrate metabolism, withonset or first recognition during pregnancy, resulting in hyperglycemia of variable severity. Insulin resistance and chronic subclinical inflammation are the underlying mechanisms of the disease. Soluble interleukin-2 receptor, neutrophil-to-lymphocyte ratio, and platelet-to-lymphocyte ratio are the markers ofinflammatory disease processes such as type 1 and 2 diabetes mellitus, hepatitis, and neoplasms.STUDY DESIGN: In our study, we measured complete blood count, serum soluble interleukin-2 receptor, serum glucose in blood samples from 52 women with gestational diabetes mellitus and 50 pregnantwomen with normal glucose tolerance. Pregnant women who were tested for oral glucose tolerance test(75 gr load) between 24 and 28 weeks of pregnancy were selected for the study. Gestational diabetesmellitus was defined according to the criteria provided by the National Institute for Health and CareExcellence.RESULTS: In terms of age, gestational diabetes mellitus patients (mean±SD; 31±6 years) were olderthan controls (mean±SD; 25±5.3 years). Mean platelet volume values were lower in gestational diabetesmellitus patients (mean±SD; 10.3±1.4 fL) as compared to normal glucose tolerance group (mean±SD;10.8±1fL). No statistically significant differences in serum glucose concentration, white blood cell count,neutrophil count, lymphocyte count, hemoglobin concentration, platelet count, neutrophil-to-lymphocyteratio value, platelet-to-lymphocyte ratio value, and serum soluble interleukin-2 receptor concentrationwere found.CONCLUSION: This study did not reveal an increase in the inflammatory markers, neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio, and serum soluble interleukin-2 receptor, in gestational diabetes mellitus. The mean platelet volume values were observed to be lower in gestational diabetes mellitus patients.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2019Increase of endocan, a new marker for inflammation and endothelial dysfunction, in acute kidney injury
Gunay, Murat; Mertoglu, Cuma
OBJECTIVE: In this study, the clinical relevance of the levels of serum endocan and 25-hydroxyvitamin D [25(OH)D] was investigated in patients with acute kidney injury (AKI). Endocan or the endothelial cell-specific molecule 1 is a soluble proteoglycan secreted by vascular endothelial cells. It plays a significant role in immunity, inflammation, and endothelial function. METHODS: A total of 39 patients with AKI (19 females, 20 males) and 38 healthy individuals (18 females, 20 males) were included in the study. The levels of serum endocan, vitamin D, and other biochemical parameters were compared between the two groups. RESULTS: In the AKI group, the values of serum creatinine, endocan, parathormone, phosphorus, and uric acid were found to be higher, and the total protein, albumin, and calcium levels were lower compared to the control group. There was no difference between the two groups in terms of the serum vitamin D, magnesium, alkaline phosphatase, and gamma-glutamyl transferase. CONCLUSION: In patients with AKI, an increased endocan level is a significant marker of inflammation and endothelial injury. In addition, these patients experience vitamin D deficiency.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2019Analysis of preanalytical errors by different autoanalyzer and sample types
Mertoglu, Cuma
Aim: In this study, it was aimed to determine the causes of preanalytical errors according to the different autoanalyzer and sample types. Material and Method: The rejected biological samples were analyzed in the laboratory information system of Mengucek Gazi Training and Research Hospital between January 1, 2017 and December 31, 2017. According to device types, reasons for rejection and rejection rates were identified. Results: At the indicated dates, 748758 samples were reached in the laboratory, of which 4245 (0.56 %) were rejected. The reasons for rejection are mostly following: incorrect test request (53.6 %) and hemolysis (22.6 %) in the biochemistry samples, inadequate sample (61.5 %) and incorrect test request (30.2 %) in hormone samples, clotted samples (76.9 %) and incorrect sample container (7.9 %) in hemogram samples, level error (38.5 %) and clotted sample (31.3%) In coagulation samples, clotted sample (89.1%) and inadequate sample (5.7 %) in the blood gas samples, incorrect sample container (53.7 %) and incorrect test request (21.1 %) in the nephelometer samples, incorrect sample container (37.4 %) and clotted samples (34.1 %) in the sedimentation samples were identified. Discussion: The preanalytical error sources vary according to the sample types and autoanalyzers. These results should be stated in personnel training and necessary precautions should be taken.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2019Thiol–Disulfide Homeostasis and Serum Ischemia Modified Albumin Levels in Patients with Primary Open–Angle Glaucoma
Karakurt, Yucel; Mertoglu, Cuma; Gok, Gamze; Ucak, Turgay; Tasli, Nurdan; Icel, Erel; Erel, Ozcan
Aim: Glaucoma is an optic neuropathy causing progressive impairment of visual functions. We aimed to assess the alterations in thiol–disulfide homeostasis and serum ischemia modified albumin (IMA) levels, which are the determinants of antioxidant status, in patients with primary open-angle glaucoma (POAG). Material and Method: A total of 140 eyes from 70 patients with POAG and 174 eyes of 87 healthy, control cases were included in the study. All study participants underwent a complete ophthalmic evaluation. Alterations in serum thiol–disulfide homeostasis and IMA levels were measured in all participants. Results: IMA levels were significantly higher in glaucoma group (p:0.001). Native thiol, Total thiol and Native/Total thiol ratio were significantly decreased while Disulfide, Disulfide/Native thiol, and Disulfide/Total thiol ratios were significantly higher in glaucoma group (p:0.001). There was no significant difference regarding central corneal thickness (CCT), iridocorneal angle, albumin, IMA and adjusted IMA levels in patients with different stages of glaucoma. In correlation analysis, there was a negative correlation between Native thiol, Total thiol and Native/Total thiol ratio and disease period, number of daily drops required and stage of the disease in glaucoma patients. There was also a positive correlation between Disulfide, Disulfide/Native thiol and Disulfide/Total thiol levels and the disease activity. Conclusion: In patients with POAG, thiol–disulfide homeostasis is disturbed in the favor of pro-oxidant molecules and IMA levels are increased, indicating the presence of augmented oxidative stress. In that aspect, systemic and local anti-oxidant treatments may be new targets in glaucoma treatment. Larger, prospective studies about the role of anti-oxidants in prevention and treatment of POAG are warranted.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2019Thiol-Disulfide Homeostasis, Serum Ferroxidase Activity, and Serum Ischemia Modified Albumin Levels in Childhood Iron Deficiency Anemia
Topal, Ismail; Mertoglu, Cuma; Sürücü Kara, Ilknur; Gok, Gamze; Erel, Ozcan
Aim: The aim is to compare the markers of oxidative stress in iron deficient children to that of non-anemic children. Method: Serum thiol-disulfide level, ferroxidase activity and ischemia-modified albumin (IMA) levels were compared between iron deficiency anemia (IDA) and non-anemic children. Results: A total of 117 children, 66 with IDA and 51 non-anemic children were included in the study. Disulfide, disulfide/native thiol, and disulfide/total thiol levels were significantly higher in the IDA group (p: 0.001). Serum ferroxidase levels were significantly lower in the IDA group (p: 0.04); but there was no significant difference between the two groups regarding serum IMA levels (p: 0.42). There was a weak negative correlation between disulfide and serum hemoglobin (p: 0.004), iron (p: 0.041), and ferritin (p: 0.023) levels while there was a weak positive correlation between ferroxidase activity and these parameters. Conclusion: There is an increased protein oxidation in children with IDA compared with non-anemic controls.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2019Thiol-Disulfide Homeostasis, Serum Ferroxidase Activity, and Serum Ischemia Modified Albumin Levels in Neonatal Jaundice
Topal, Ismail; Mertoglu, Cuma; Surucu Kara, Ilknur; Siranli, Gulsah; Gok, Gamze; Erel, Özcan
Aim: Hyperbilirubinemia causes oxidative stress. Method: We evaluated three oxidative stress markers in hyperbilirubinemic neonates (native/total thiol levels, serum ferroxidase activity and ischemia modified albumin (IMA), comparing these levels to levels in a control group to determine which indicators were the most sensitive. Results: Serum from 124 term infants (67 with pathologic jaundice and 57 controls) were evaluated. Native/total thiol ratio was significantly lower (p:0.021) while disulfide levels were significantly higher (p:0.001) in the jaundiced group. There was no significant difference in ferroxidase (p:0.603) or IMA (p:0.251) levels. Conclusion: Altered thiol/disulfide homeostasis in the favor of disulfide indicates augmented oxidative stress in jaundiced term infants. The lack of alteration in ferroxidase or IMA levels suggests these latter alterations take more time or more severe oxidative stress to become altered or are not as sensitive as the thiol/disulfide ratio to detect oxidative stress states.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2019Could interleukin-33 and its suppressor of tumorigenicity 2 (ST2) receptor have a role in cervical human papillomavirus (HPV) infections?
Kulhan, Nur Gozde; Kulhan, Mehmet; Aydin, Merve; Nayki, Umit; Nayki, Cenk; Ulug, Pasa; Ata, Nahit; Mertoglu, Cuma; Cikman, Aytekin; Sayar, İlyas
Why most women can clear human papillomavirus (HPV) infections while others can develop permanent infections. The stimulation of immunotolerance of the immune system of the host by the persistent HPV infection may be the answer to this question. Interleukin-33 (IL-33) may play a role in the pathogenesis of HPV infection, this hypothesis was thought to be due to the rapid release of IL-33 from damaged cells following tissue damage, necrosis, and activation of the inflammasome. Thus, in this study, the role of IL-33/suppressor of tumorigenicity 2 (ST2) was emphasized in HPV positive and HPV negative cervical tissues. A total of 80 were assessed. The reduced levels of IL-33 and ST2 are associated with cervical HPV infections. There was a statistically significant 42% positive correlation between IL-33 and ST2 in the HPV-positive group. Surprisingly, our data showed no significant difference between the expression levels of IL-33 or ST2 and working status, type of delivery, pre- and post-operative pathology, cigarette, educational status, locality, birth control method, gynecological, and colposcopic findings. We found that as a result of our study; low IL-33 and ST2 levels were associated with HPV infections.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2018Erzincan Bölgesindeki Çocukların D Vitamini Seviyelerinin Yaş, Cinsiyet ve Mevsimlere Göre Değerlendirilmesi
Arslan, Yusuf Kemal; Mertoglu, Cuma; Topal, İsmail; Sürücü Kara, İlknur; Peker, Necla; Gümüş, Alper
Amaç: D Vitamini, vücutta kalsiyum ve fosfor metabolizmasını düzenleyen, büyüme, gelişme ve sağlıklı bir iskelet yapısı için önemli bir hormondur. D vitamini eksikliği kemik dokusunun etkileri dışında birçok hastalıkların oluşmasında rolü olduğu bildirilmiştir. Bu çalışmada, Erzincan ilindeki çocuklarda D vitamini düzeylerinin retrospektif olarak aylara, cinsiyete, yaşa ve mevsimlere göre dağılımının değerlendirilmesi amaçlanmıştır. Gereç ve Yöntem: Çalışmamızda Ocak 2013–Aralık 2016 tarihleri arasında hastanemiz çocuk polikliniğine başvuran ve D vitamini ölçümü yapılan 2346 sonuç dâhil edildi. Hastalar Stage 6 kriterine bağlı olarak oluşturulan yaş gruplarına, cinsiyet ve 25 (OH ) D vitamini düzeyleri ay ve mevsimlere göre gruplara ayrıldı. Bulgular: Sonuçların %8,36’sı 10 ng/ml nin altında olup ciddi eksiklik grubunda,%42,33 20ng/ml altında olup hafif-orta derecede eksiklik ve %27,27 ng/ml olup yetersizlik grubunda yer almaktaydı. Erkeklerin kızlara göre daha yüksek D vitamini düzeyine sahip oldukları, yaş ilerledikçe D vitamini düzeylerinin azaldığı ve en yüksek D vitamini düzeyine yaz ayında en düşük D vitamini düzeyine ise kış ayında rastlanıldığı görülmüştür. Sonuç: İki yaş altı çocuklar hariç, tüm çocuklarda D vitamini düzeyi yetersizdir. Dolayısıyla bu yaş grubunda yeterli D vitamini düzeyi sağlamak için gerekli önlemler alınmalıdır. Hastalarda D vitamini düzeylerinin cinsiyete, yaşa ve mevsimlere bağlı olarak değişiklik gösterdiği sonucuna varılmıştır (p <0,001).
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2018Vitamin D supplementation does not improve plasma thiol/disulfide homeostasis
Mertoglu, Cuma; Siranli, Gulsah; Topal, Ismail; Gok, Gamze; Erel, Ozcan
Background: Impairment of thiol/disulfide homeostasis, as well as vitamin D deficiency, are responsible for the pathophysiology of many acute and chronic diseases. This study examined the relationship between thiol/disulfide homeostasis and vitamin D level and supplementation. Methods: A total of 203 healthy children were included in the study. The participants were divided into four groups according to 25-hydroxyvitamin D (25(OH)D) level: group 1, severe deficiency (<10 ng/mL); group 2, deficiency (10–20 ng/mL); group 3, insufficiency (20–30 ng/mL); and group 4, sufficiency (≥30 ng/mL). Furthermore, group 5 was defined as being on vitamin D supplementation. Result: Native thiol was lower in group 5 than in groups 2–4 (P = 0.003). Disulfide was higher in groups 1, 4 and 5 than groups 2 and 3 (P < 0.001). Total thiol was lower in group 5 than in group 4 (P = 0.032). The ratio of native thiol/total thiol was lower in groups 1 and 5 compared with groups 2 and 3, and in group 4 compared with group 3 (P < 0.001). The ratios of disulfide/total thiol and disulfide/native thiol were higher in groups 1 and 5 than in groups 2 and 3 whereas only the disulfide/total thiol ratio was higher in group 4 than in group 3 (P < 0.001). Conclusions: In healthy children, severe deficiency of vitamin D causes impairment of thiol/disulfide homeostasis and increases protein oxidation, which cannot be reversed by external vitamin D supplementation.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2018Ischemic modified albumin increases in acute kidney injury
Mertoglu, Cuma; Gunay, Murat; Gurel, Ali; Gungor, Mehmet; Gul, Vahdet
Purpose: Acute kidney injury (AKI) is a severe kidney disease carrying high morbidity and mortality. An ischemic process, at the cellular level, has been detected prior to the full-blown AKI. An elevated ischemic modified albumin (IMA) was also found to be increased fast at several minutes following an ischemic process in the body. In this connection, we have investigated, in advance, the changes of IMA concentrations in patients with possible AKI. Methods: IMA and other biochemical and haematological parameters were measured in sera of thirty nine patients with AKI and of thirty eight healthy controls. AKI is defined by an increase in serum creatinine by ≥ 0.3 mg/dl in 48 hours or an increase by ≥ 1.5-fold from a known or assumed baseline. The results in the two groups were compared. Results: IMA, creatinine, blood urea nitrogen, white blood cell, neutrophil, neutrophil–lymphocyte ratio (NLR), platelet–lymphocyte ratio (PLR) and mean platelet volume were found to be higher in patients with AKI than in healthy controls. In contrast, total protein, albumin, lymphocyte, and haemoglobin were lower in patients with AKI than in healthy controls. No significant difference was recorded in platelet counts between the two groups. Conclusion: Our results indicate that increased levels of NLR and PLR play a central role in a systemic inflammation in AKI. Monitoring serum IMA could be a useful tool in the assessment of AKI.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2018Does extracorporeal shockwave lithotripsy therapy affect thiol-disulfide homeostasis?
Bozkurt, Aliseydi; Mertoglu, Cuma; Karabakan, Mehmet; Siranli, Gulsah; Yurt, Emine Feyza; Erel, Ozcan
Objective: Extracorporeal Shockwave Lithotripsy (ESWL) is a non-invasive method that is effective at crushing stones in the upper urinary tract. Disturbance of the thiol/disulfide homeostasis, in favor of the disulfide, has been shown to be involved in the disease pathogenesis. Methods: A total of 36 individuals that underwent ESWL had blood samples collected before ESWL (0hrs), 6hrs, and one week after the ESWL. Sera native and total as wells as disulfide amount was measured using an automated method sodium borohydrate (NaBH4) reduction. In addition, Ischemia Modified Albumin (IMA) levels were measured using colorimetric assay method. Results: Native thiol level was reduced at the 6th hour following ESWL compared to baseline. While the ratios of disulfide level, Disulfide/Total Thiol (DTT), Disulfide/Native Thiol (DNT) and IMA level were increased at the 6th hour following ESWL compared to baseline, they were found to be similar with their baseline values at the end of 1st week. Total thiol and native/total thiol did not show any significant change. Conclusions: ESWL treatment disrupts thiol/disulfide homeostasis and the structure of albumin at the acute term. Therefore, it increases protein oxidation and leads to increased oxidative stress. However, this state is transient and returns to normal within the proceeding days.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2018Could endocan, a marker of inflammation and endothelial dysfunction, be a new diagnostic marker for fibromyalgia?
Mertoglu, Cuma; Gunay, Murat; Yerligok, Onur
Background: Although the pathophysiology of fibromyalgia has not yet been fully clarified, inflammation and endothelial dysfunction have been suggested to occur in patients with fibromyalgia. In recent years, endocan has been reported as an important biomarker for inflammation and endothelial dysfunction. In this study, we investigated this mechanism by measuring the serum endocan levels in fibromyalgia patients. Methods: The serum samples collected from 37 female patients diagnosed with fibromyalgia for the first time and 43 healthy women were analysed in terms of the endocan levels and laboratory parameters, and the values from the two groups were compared. Results: In the patient group, the serum endocan level was found to be higher (p < 0.0001). Endocan had a significantly high sensitivity and specificity for the diagnosis of fibromyalgia (area under curve: 0.897, cutoff value: 9.81 ng/mL, sensitivity: 88.5%, specificity: 89.7%). Conclusions: Subclinical inflammation and endothelial dysfunction are important in the pathophysiology of fibromyalgia. Increased endocan not only elucidates this mechanism but also presents as an important potential marker for fibromyalgia.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2018Does myo-inositol oxygenase, the only enzyme to catalyze myo-inositol in vivo, play a role in the etiology of polycystic ovarian syndrome?
Mertoglu, Cuma; Gunay, Murat; Gul, Vahdet; Kulhan, Mehmet; Aktas, Mehmet; Coban, Taha Abdulkadir
In polycystic ovary syndrome (PCOS), myo-inositol (MI) supplements have shown many beneficial effects. In this study, therefore, we aimed to investigate the serum level of myo-inositol oxygenase (MIOX), which is the only enzyme catalyzing MI in vivo, in patients with PCOS. Serum MIOX enzyme levels and other laboratory parameters were compared between sixty patients, who were diagnosed with PCOS for the first time, and sixty healthy individuals at similar age and sex. MIOX serum levels were not different between two groups (p = 0.7428). MIOX median and 95% CI were 19.4 and 10.6–39.1 in the control group and 16.4 and 7.6–46.2 in the patient group respectively. Demographic data, biochemical and hematological parameters, hormone parameters were not different except from the lymphocyte count between the two groups. Lymphocyte count was higher in the patient group. Although the ratio of LH/FSH was higher in the patient group, it was not statistically significant. Our results suggest that serum MIOX levels do not change in PCOS. It was, therefore, concluded that MI deficiency observed in PCOS was not related to the level of MIOX enzyme which cleaves MI.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2018Evaluation of the effect of retrograde intrarenal surgery with myo-inositol oxygenase
Mertoglu, Cuma; Bozkurt, Aliseydi; Keskin, Ercüment; Gunay, Murat
Objective: To investigate the effect of retrograde intra-renal surgery (RIRS) on kidneys using the myo-inositol oxygenase (MIOX) enzyme. MIOX is a renal tubular-specific novel marker for the early diagnosis of acute kidney injury. Methods: A total of twenty seven individuals that had undergone RIRS to treat kidney stones were included in the study. Biochemical tests were performed on serum samples collected immediately before RIRS (hour 0) and at the 6th and 24th hours after the surgery. Results: The creatinine value at hour 6 was lower than the baseline (hour 0) value (p = 0.0305). Cystatin C at hour 6 was lower than the value measured at hour 24 (p = 0.0142). Similarly, MIOX was lower at hour 6 compared to hour 24 (p = 0.0214). MIOX/creatinine at hour 6 was lower than the value calculated at hour 24 (p = 0.0348). The basal values of MIOX and creatinine were found to have a positive correlation (correlation coefficient r = 0.5946, p = 0.0035). Conclusions: Similar to the serum creatinine, the serum MIOX level provides information about kidney functions. RIRS was confirmed to be a safe procedure for the treatment of acute kidney injury with no negative effects on the kidneys.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2018Myo-inositol oxygenase as a novel marker in the diagnosis of acute kidney injury
Mertoglu, Cuma; Gunay, Murat; Gurel, Ali; Gungor, Mehmet
Background: Due to the lack of diagnostic efficiency of serum creatinine in acute kidney injury (AKI), there is a pressing need to develop novel diagnostic markers. Therefore, in this study, we evaluated myo-inositol oxygenase (MIOX), neutrophil gelatinase-Associated lipocalin (NGAL) and cystatin C in terms of their applicability in the diagnosis of AKI. Methods: We enrolled a total of 39 AKI patients and 38 healthy controls in the study. We compared the levels of serum MIOX, NGAL and cystatin C between the two groups. Results: We found that the concentrations of serum creatinine, blood-urea nitrogen, MIOX and cystatin C were higher in the AKI group. According to the receiver operating characteristic analysis, the area under the curve (AUC) values were 0.694 (95% CI 0.579-0.794) for MIOX and 0.976 (95% CI; 0.912-0.997) for cystatin C. For MIOX, when the cut-off concentration was set to 77.3 pg/mL, the diagnostic sensitivity and specificity were found to be 53.8% (95% CI; 37.2-69.9) and 81.5 (95% CI; 65.7-92.3), respectively. For cystatin C, at the cut-off value of 14 mg/L, the diagnostic sensitivity and specificity were 94.8% (95% CI; 82.7-99.4) and 94.7 % (95% CI 82.3-99.4), respectively. Conclusions: The measurement of serum MIOX and cystatin C levels is valuable for the diagnosis of AKI. Further research is needed for the evaluation of the potential use of MIOX as a kidney-specific enzyme in the early diagnosis of AKI.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2017Vitamin D Level in Pregnancy, Gestational Diabetes and Women of Reproductive Age
Kulhan, Mehmet; Mertoglu, Cuma; Günay, Murat
Objective: In this study, we aimed to determine vitamin D levels in healthy gestations, gestational diabetic pregnancies and healthy women during the reproductive age. Materials and Methods: Vitamin D was measured immunochemically from sera from 28 healthy pregnant women, 15 gestational diabetic pregnant women and 30 healthy young adult women ages 18-43 year. Results: Vitamin D deficiency was found in all groups [healthy pregnancies (11.8±7.4), gestational diabetics (10.6±5.7), and healthy young women (12.1±5.4) ng/mL]. There is no significant difference between the groups (p=0.54). Conclusion: Vitamin D level is not sufficient in all pregnancies and young adult women. So vitamin D supplementation should be performed.
RDL Kalıcı Kaydıİncele