Synthesis and characterization of 2,3-diaminophenazine@CuhNFs and 2,3-diaminopyridine@CuhNFs: a new strategy to enhance anticancer activity

In this study, synthesis and characterization of 2,3-diaminophenazine@CuhNFs and 2,3-diaminopyridine@CuhNFs were carried out. The morphologies of the synthesized CuhNFs were investigated using Fourier Transform Infrared spectrometry, scanning electron microscopy and energy dispersive X-ray, elemental mapping, and XRD spectroscopy. Organic compounds and synthesized nanoflowers were screened against human lung (A549) and breast (MCF-7) cancer cell lines. The results show that the tested compounds had cytotoxic activity in both cell lines. To support the in vitro part of the study, the MTT results were also examined by in silico approaches. The binding parameter values of compounds interacting via in silico with 1M17 and 3ERT crystal structures in molecular docking and important amino acids in the binding site were determined. The pharmacokinetic properties and toxicity of compounds were evaluated using ADMET analysis. © 2026 Taylor & Francis Group, LLC.