Araştırmacılar(32)

• Alptekin, Okay
• Arslan Nayki, U.
• Ata, Nahit
• Dinc, Kemal
• Kalkan Yilmaz, Betul
• Kalkan, Betul
• Kaplan, S.
• Kiremitli, Sevil
• Kiremitli, Tunay
• Kirkinci, Ahmet
• Kozali Uzun, Sukran
• Kulhan, M.
• Kulhan, N.G.
• Kurnuç, Fatma Zehra
• Narin, Mehmet Ali
• Nayki, C.
• Nayki, Umit
• Nayki, Usmit
• NG, Kulhan
• Onat, T.
• Oner, Gokalp
• Paltacı, Şeyma İlayda
• Pasa, Ulug
• Toklucu, Hulya
• Turkler, Can
• Ulug, Pasa
• Ulug, S.
• Yavuz, Adem
• Yılmaz, Nesrin
• Yildinm, Yusuf
• Yildirim, E.
• Yildirim, Yusuf

• ArticleAçık Erişim2026/r/ebyu/11226
  Comparative Protective Effects of ATP and TPP Against Olaparib-Induced Ovarian Injury in Rats: Insights from Biochemical Parameters, Histopathology, and IRE1α, Cleaved Caspase-3, LC3B, and NF-κB p65 Double Immunofluorescence

  Paltaci, Seyma Ilayda; Yilmaz, Nesrin; Kozali Uzun, Sukran; Yavuzer, Bulent; Coban, Taha Abdulkadir; Ozkaraca, Mustafa; Gungor, Ali; Yaqubov, Kamandar; Suleyman, Halis

  Background: Olaparib is a poly(ADP-ribose) polymerase (PARP) inhibitor used to treat several malignancies. Previous studies have reported that olaparib may induce ovarian injury by elevating reactive oxygen species and impairing cellular energy homeostasis. ATP is a central regulator of intracellular energy balance and antioxidant defense, whereas thiamine pyrophosphate (TPP), the active form of vitamin B1, supports ATP biosynthesis and possesses antioxidant and anti-inflammatory properties. This study compared the protective effects of ATP and TPP against olaparib-induced ovarian injury in rats. Methods: Twenty-four female Wistar albino rats were randomly assigned to four groups (n=6 each): healthy control (HG), olaparib-only (OLP), ATP plus olaparib (ATOL), and TPP plus olaparib (TPOL). Olaparib (100 mg/kg, orally, twice daily), ATP (5 mg/kg, intraperitoneally, once daily), and TPP (25 mg/kg, intraperitoneally, once daily) were administered for 14 consecutive days. Ovarian tissue levels of oxidant (MDA), antioxidants (tGSH, SOD, CAT), and pro-inflammatory cytokine (IL-6) were determined. Tissue specimens underwent histopathological (follicular and parenchymal degeneration) and double immunofluorescence analyses (IRE1α, cleaved Caspase-3, LC3B, and NF-κB p65 immunopositivity). Results: Olaparib significantly increased oxidant and pro-inflammatory cytokine levels and decreased antioxidant levels. Histopathology revealed severe follicular and parenchymal degeneration, and immunofluorescence analysis showed significantly increased IRE1α, cleaved Caspase-3, LC3B, and NF-κB p65 immunopositivity. Both ATP and TPP pre-treatment significantly attenuated olaparib-induced oxidative stress, inflammatory responses, histopathological injury, and cellular stress marker immunopositivity. ATP demonstrated a more pronounced protective effect than TPP, particularly regarding follicular degeneration suppression and LC3B and NF-κB p65 immunopositivity. Conclusion: Olaparib induced ovarian injury accompanied by oxidative stress, cytokine-mediated inflammation, IRE1α-related endoplasmic reticulum stress, cleaved Caspase-3-related apoptosis, LC3B-related autophagy, and NF-κB-related inflammatory signaling. Both ATP and TPP attenuated olaparib-associated injury, with greater effects for ATP. These findings suggest that maintenance of cellular energy homeostasis may represent a promising strategy for attenuating PARP inhibitor-induced ovarian injury.

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• ArticleYayıncıda açık erişim2026/r/ebyu/10775
  Protective Effects of Adenosine Triphosphate and Flunarizine on Erlotinib-Induced Ovarian Damage: An Experimental Study

  Yavuz, Arzu; Uzel, Kemine; Sezgin, Esra Tuba; Kuzucu, Mehmet; Yılmaz, Nesrin; Yazici, Gülcenaz; Hendem, Engin; Süleyman, Halis

  (1) Background: Erlotinib is a tyrosine kinase inhibitor (TKI) widely used in cancer therapy; however, its potential adverse effects on ovarian tissue have not been fully elucidated. The present study aimed to investigate erlotinib-induced ovarian injury and to evaluate the protective effects of adenosine triphosphate (ATP) and flunarizine, administered alone or in combination, using biochemical and histopathological analyses in a rat model. (2) Methods: Thirty female rats were randomly allocated into five groups (n = 6 per group): healthy control, erlotinib, ATP + erlotinib, flunarizine + erlotinib, and ATP + flunarizine + erlotinib. ATP (5 mg/kg, intraperitoneal) and flunarizine (5 mg/kg, oral gavage) were administered daily for two weeks, while erlotinib (5 mg/kg) was given orally every two days for two weeks. Ovarian tissues were collected for oxidative stress analysis and histopathological evaluation, and blood samples were obtained for the measurement of serum prolactin and AMH levels. (3) Results: Erlotinib administration resulted in significant oxidative stress and histopathological alterations in ovarian tissue, accompanied by a reduction in serum AMH levels, while prolactin levels remained unchanged. Treatment with ATP or flunarizine partially attenuated these alterations. (4) Conclusions: Combined ATP and flunarizine administration showed stronger protective effects, improving biochemical parameters and preserving ovarian histology, suggesting a protective role against erlotinib-induced ovarian injury.

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• ArticleAçık Erişim2026/r/ebyu/10432
  Flunarizine attenuates ischemia–reperfusion–induced ovarian injury in rats

  Yavuz, Arzu; Uzel, Kemine; Sezgin, Esra Tuba; Paltacı, Şeyma İlayda; Demir, Özlem; Kuzucu, Mehmet; Süleyman, Halis

  Background: Ovarian ischemia–reperfusion (I/R) injury is associated with oxidative stress, inflammation, and histopathological damage. The aim of this study was to investigate the potential protective effects of flunarizine against ovarian I/R–induced injury in an experimental rat model. Methods: Rats were divided into four groups: healthy control (HG), sham-operated (SOG), ovarian ischemia–reperfusion (OIR), and flunarizine-treated OIR (FOIR). Ovarian tissues were analyzed in terms of oxidative stress markers, antioxidant enzyme activities, inflammatory cytokines, and histopathological alterations, including follicle counting. Results: Ovarian I/R significantly increased tissue levels of malondialdehyde (MDA), tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6) while significantly decreasing total glutathione (tGSH), superoxide dismutase (SOD) and catalase (CAT) levels. Histopathological evaluation revealed severe follicular degeneration, interstitial edema, vascular congestion, and inflammatory cell infiltration in the OIR group. Following flunarizine administration, these biochemical and histopathological alterations were significantly ameliorated, and a partial restoration in follicle counts was observed. Conclusion: The findings of this study demonstrate that flunarizine exerts protective effects against ovarian I/R injury by reducing oxidative stress, inflammation, and histopathological damage.

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• ArticleYayıncıda açık erişim2026/r/ebyu/10092
  Double-Convex Peroneal Tubercle Morphology and MRI-Detected Peroneal Tendon Abnormality in a Non-Lateral Referral Cohort

  Gür, Volkan; Gökgöz, Mehmet Burak; Aydın, Abdurrahman; Issın, Ahmet; Can, Muhammet Ali; Yanmaz, Oğuzhan; Koçkara, Nizamettin; Yapıcı, Furkan

  Background: The peroneal tubercle demonstrates substantial morphologic variability and may influence peroneal tendon mechanics. This study evaluated whether peroneal tubercle morphology and size are associated with MRI-detected peroneal tendon abnormality in patients undergoing ankle MRI for ankle pain without documented lateral malleolar/retromalleolar or peroneal tendon-specific symptoms. Methods: In this retrospective cross-sectional study, 487 ankle MRI examinations obtained between 2020 and 2023 were analyzed after excluding cases with lateral ankle/peroneal symptoms, clinically significant acute trauma, prior ankle surgery, or systemic inflammatory disease. Two orthopedic surgeons independently classified peroneal tubercle morphology (single-convex vs. double-convex) and measured tubercle height and anteroposterior length. Peroneal tendon abnormality was defined as MRI features consistent with tendinopathy and/or tenosynovitis. Inter- and intraobserver reliability were assessed using intraclass correlation coefficients and Cohen’s kappa. Unadjusted associations were assessed using Fisher’s exact test and point-biserial correlation. Multivariable logistic regression was performed to evaluate independent associations after adjustment for age, sex, BMI, and hindfoot alignment. Results: Mean age was 43.6 ± 15.6 years; 226 participants were male, and 261 were female. Peroneal tendon abnormality was present in 227/487 examinations (46.6%). Double-convex morphology showed a higher prevalence of abnormality than single-convex morphology (69.6% vs. 43.6%; unadjusted OR 2.97, 95% CI 1.63–5.41; p < 0.001). In the adjusted model, double-convex morphology remained independently associated with peroneal tendon abnormality (OR 2.85, 95% CI 1.52–5.34; p = 0.001). Tubercle height showed a modest independent association (OR 1.12 per mm, 95% CI 1.04–1.20; p = 0.002), whereas tubercle length was not associated (OR 1.008, 95% CI 0.981–1.036; p = 0.541). Reliability was excellent for tubercle height, tubercle length, and hindfoot alignment measurements and substantial to excellent for categorical ratings. Conclusions: In this non-lateral referral cohort, double-convex peroneal tubercle morphology was independently associated with higher odds of MRI-detected peroneal tendon abnormality. These findings reflect cross-sectional imaging associations rather than causation and should be interpreted with caution, given the heterogeneous MRI endpoint and the routine clinical MRI protocol.

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• ArticleYayıncıda açık erişim2025/r/ebyu/19584
  Evaluation of Sexual Life According to Pregnancy Trimesters

  Yılmaz, Betül Kalkan; Meydan, Begüm Naz

  EVALUATION OF SEXUAL LİFE ACCORDİNG TO PREGNANCY TRİMESTERS ABSTRACT Aim: Changes that occur during pregnancy physiologicaly; affects the couple's sexual life. Sexuality, which is an important part of health, is disrupted during pregnancy due to some beliefs and taboos. With the strong influence of hormones, changes in sexual desire and arousal are inevitable. The aim of the study was to evaluate the sexual function levels of pregnant women according to trimester periods. Materials and Methods: The study was conducted prospectively; Those who applied to the Gynecology and Obstetrics polyclinic in between November 2023 and December 2023; Women whose pregnancy was between 6 and 40 weeks were included. After obtaining consent from patients who met the inclusion and exclusion criteria, the 'Female Sexual Function Index-FSFI' questionnaire was administered face to face and age, parity, educational status, employment status, and body mass indexes were calculated and recorded. Results: A total of 198 pregnant women between 6-40 weeks of gestation who applied to the gynaecology and obstetrics outpatient clinic accepted to participate in the study. Of the pregnant women who participated in the study, 64 were in the first trimester, 64 were in the second trimester and 67 were in the third trimester. When the groups were compared, 93.75% of the first trimester pregnant women, 93.75% of the second trimester pregnant women and 98.5% of the third trimester pregnant women were diagnosed with sexual dysfunction and the rate of sexual dysfunction was significantly higher in the third trimester. Conclusion: One of the important factors affecting the quality of life and relationship of women is to have a healthy sexual life. During pregnancy, interest in sexual intercourse decreases in most women as pregnancy progresses. This study also showed that sexual function problems of women increase in the first and last trimesters of pregnancy; sexual desire and satisfaction with sexuality, arousal and orgasm decreases. Key words: pregnancy, sexual function, trimester

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• ArticleAçık Erişim2025/r/ebyu/18746
  Biochemical and Histopathologic Investigation of the Effect of Rilmenidine on Ovarian Ischemia-Reperfusion Injury in Rats

  Yilmaz, Betul Kalkan; Bulut, Seval; Yilmaz, Nesrin; Yucel, Nurinisa; Cicek, Betul; Kadioglu, Berrin Goktug; Bakan, Nuri; Mendil, Ali Sefa; Suleyman, Halis

  Background and Objective: Rilmenidine is an antihypertensive drug with sympatholytic properties as well as antioxidative and anti-inflammatory properties. To investigate the biochemical and histopathological effects of Rilmenidine on Ovarian IschemiaReperfusion (I/R) injury in a rat model, this study aims to determine its potential protective role against oxidative stress and tissue damage associated with ovarian IR injury. Materials and Methods: The 24 albino Wistar female rats were divided into four groups: Healthy (HG), sham-operation (SOG),ovarian ischemia-reperfusion (OIRG) and Rilmenidine+ovarian ischemia-reperfusion (ROIRG). Ovaries in SOG, OIRG and ROIRG groups were exposed to ischemia for three hrs, followed by reperfusion for 6 days. The ROIRG rats were treated with Rilmenidine (0.2 mg/kg) daily for 6 days before ischemia. The ovaries were analyzed for oxidant, antioxidant and proinflammatory cytokines and examined histopathologically. Statistical analysis was performed using one-way ANOVA and Kruskal-Wallis tests (p<0.05). Results: The IR procedure increased tissue malondialdehyde, tumor necrosis factor-a, interleukin-6 and interleukin-1$ levels and decreased total glutathione, superoxide dismutase and catalase levels (p<0.001). Severe histopathologic damage and 8-hydroxy-20deoxyguanosine immunopositivity were also observed in ovarian tissues ofOIRG (p<0.001). Rilmenidine inhibited IR-related biochemical, histopathological and immunohistochemical abnormalities (p<0.05). Conclusion: Rilmenidine may be an efficient therapeutic strategy for the protection from ovarian IR injury.

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• ArticleYalnız künye2025/r/ebyu/11803
  Hormonal mechanism and pathogenetic therapy of citalopram-induced infertility in female rats

  Delibasi, Ilhan Bahri; Gumusburun, Neset; Bulut, Seval; Mammadov, Renad; Yilmaz, Betul Kalkan; Suleyman, Bahadir; Bakan, Nuri; Mendil, Ali Sefa; Suleyman, Halis; Altuner, Durdu

  Citalopram is a selective serotonin reuptake inhibitor (SSRI) and has been associated with reproductive dysfunction in women. In this study, the effects of citalopram on reproductive health in female rats were investigated. Albino Wistar rats was divided into six groups (each group/n = 12): healthy (HG), citalopram (CTP), cabergoline (CBR), metyrapone (MTP), cabergoline+citalopram (CBR+CTP), and metyrapone+citalopram (MTP+CTP). Initially, cabergoline 0.1 mg/kg and metyrapone 50 mg/kg were administered orally. A dose of 10 mg/kg of citalopram was given orally one hour later. For 30 days, the treatment protocol was applied once a day. Then, blood samples were taken from the tail veins of six rats from each group for prolactin and corticosterone analyses and ovaries were removed after euthanasia. The ovaries were examined for oxidants and antioxidants and histopathologically. During two months, the remaining animals were kept with male rats. The rats that did not deliver during this period were considered infertile. In terms of oxidants and antioxidants, there was no significant difference between the groups (p > 0.05). In half of the female rats, citalopram caused infertility, increased levels of prolactin and corticosterone, and damaged the ovaries histopathologically (p < 0.05). Cabergoline suppressed the elevation of prolactin by citalopram (p < 0.001) but did not prevent infertility. In contrast, metyrapone significantly prevented the citalopram-induced increase in corticosterone, infertility, and tissue damage induced by citalopram (p < 0.05). According to the results of our study, the preventive effect of drugs that suppress excessive corticosterone on citalopram-induced infertility in rats may be encouraging for further clinical studies.

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• ArticleYayıncıda açık erişim2025/r/ebyu/11620
  Association of Tramadol-Induced Ovarian Damage and Reproductive Dysfunction with Adenosine Triphosphate and the Protective Role of Exogenous ATP Treatment

  Gumusburun, Neset; Delibasi, Ilhan Bahri; Bulut, Seval; Suleyman, Halis; Kalkan Yilmaz, Betul; Coban, Taha Abdulkadir; Mendil, Ali Sefa; Suleyman, Zeynep

  Background: Tramadol, a weak opioid analgesic agent, is known to induce ovarian damage. Previous studies have held oxidative stress responsible for the adverse effects of tramadol on female reproduction. This study examined the protective effects of ATP against tramadol-induced ovarian damage and reproductive dysfunction in rats. Methods: Rats were divided into four groups (n = 12); healthy (HG), only ATP (ATPG), only tramadol (TMDG), and ATP + tramadol (ATMG). ATP was injected intraperitoneally at 25 mg/kg. Tramadol at 50 mg/kg was initiated one hour after ATP. The treatment was administered once a day for 14 days. Six rats from each group were euthanized. For two months, the remaining rats were paired with male rats. Rats that failed to give birth during this period were considered infertile. A maternity period was calculated for the rats that were delivered. Results: Tramadol caused an increase in malondialdehyde and interleukin-6, and decreased total glutathione, superoxide dismutase, and catalase levels in the ovarian tissue. Furthermore, tramadol disrupted the histological structure of the ovaries, and immunohistochemical staining revealed severe immunopositivity. Tramadol again caused infertility and delayed pregnancy in fertile women. By suppressing biochemical changes, ATP significantly reduced tramadol-induced ovarian damage. Both histopathologically and immunohistochemically, ATP treatment regressed ovarian damage. Additionally, ATP significantly reduced tramadol-induced infertility and maternal delay. Conclusions: The results indicate that tramadol-induced oxidative and inflammatory ovarian injury, infertility, and caspase 3 were suppressed by ATP, as demonstrated by our experimental findings.

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• ArticleAçık Erişim2025/r/ebyu/11020
  The molecular mechanism of the protective effect of tianeptine against ovarian ischemia-reperfusion injury

  Kalkan, Betul; Yavuzer, Bulent; Aliyev, Aydin; Cakir, Osman Kagan; Gozen, Fusun; Mendil, Ali Sefa; Coban, Taha Abdulkadir; Suleyman, Halis

  Torsion of the ovary is a gynecologic emergency that occurs when the ovary and fallopian tube rotate around the infundibulopelvic ligament, causing ischemic changes in the adnexa. Tianeptine is an atypical antidepressant that has been shown to be effective in treatment-resistant depression and has fewer side effects than other antidepressants. In this study, the protective effect of tianeptine on ovarian ischemia-reperfusion (I/R) injury in female rats was evaluated. Four groups were created from twenty-four female albino Wistar rats before surgery (n = 6/each group): TPG (5 mg/kg/orally tianeptine-only), SG (sham operation), OIR (ovarian I/R), and TOIR (5 mg/kg/orally tianeptine + I/R). One hour before surgery procedures, the TPG and TOIR applied tianeptine. Tianeptine administration procedure was repeated once daily for six days. Under 60 mg/kg intraperitoneal (IP) ketamine and sevoflurane (inhaler) anesthesia, the right ovaries, fallopian tubes, and vessels in the OIR and TOIR groups were clamped by torsion. After 3 h of ischemia, a 6-day reperfusion period was started. Tianeptine administration procedure was repeated once daily for six days. In the SG group, the abdominal cavity was opened and closed with sutures without any intervention on the ovaries. On the seventh day, rats were sacrificed (120 mg/kg ketamine, IP), and ovaries were extracted. Levels of oxidants (malondialdehyde), antioxidants (total glutathione, superoxide dismutase, and catalase), and pro-inflammatory cytokines (tumor necrosis factor-alpha, interleukin-1β, interleukin-6) were measured in excised ovarian tissue. Ovarian tissues also examined histopathologically and immunohistochemically. I/R increased the levels of oxidants and pro-inflammatory cytokines and decreased the levels of antioxidants in rat ovaries. In parallel, histopathologic and immunohistochemical examination of the ovarian tissue revealed the presence of severe (grade 3) oxidative damage. Tianeptine significantly suppressed oxidative stress and pro-inflammatory cytokine increases related with I/R in the ovarian tissue, while also alleviating histopathological damage and oxidative DNA damage. Our experimental results suggest that tianeptine treatment may be effective against I/R-induced ovarian injury.

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• ArticleYayıncıda açık erişim2024/r/ebyu/19185
  Birinci Trimesterde Subkoryonik Hematomu olan Gebelerin Obstetrik Komplikasyonlarının ve sonuçlarının Değerlendirilmesi

  Kurnuç, Fatma Zehra; Naykı, Ümit Arslan; Balsak, Deniz; Balkan, Nazenin

  Amaç: Bu çalışmada, birinci trimesterde ultrasonografide subkoryonik hematomu olan gebelerin obstetrik komplikasyonlarının ve sonuçlarının değerlendirilmesi amaçlanmıştır. Yöntemler: Çalışmamıza, Mengücek Gazi Eğitim ve Araştırma Hastanesi Kadın Doğum Polikliniği’nde Ocak 2019 ile Kasım 2021 tarihleri arasında tekil gebeliği olan, servikal açıklığı bulunmayan ve ultrasonografide kanama alanı izlenen 14. gebelik haftasının altındaki hastalar dahil edilmiştir. Ultrasonografik olarak kanama alanı tespit edilen hastalar, takiplerinde vajinal kanama, pelvik ağrı olup olmamasına, kanama alanlarının lokalizasyonuna, hematom alanı hesaplamasına ve CRL (baş popo mesafesi) ile hematom boyutlarının oranlamasına göre gruplandırılarak her grubun ayrı ayrı gebelik prognozunu araştırmak için geçmiş hasta dosya kayıtları incelendi. Araştırma kapsamında ilk olarak hematom tespit edilen 1819 hasta tarandı ve ultrasonografide kanama alanı izlenen 334 hasta tespit edildi. Takiplerine devam etmeyen, gebeliği hastanemizde sonlanmayan ve belirtilen risk faktörleri olan hastalar dışlandı ve araştırma kapsamına 215 hasta alındı. Tüm veriler istatistiksel olarak analiz edildi. Bulgular: Hastaların komplikasyon oranı %60,9 olarak bulunmuştur. Komplikasyonlardan en sık %43,7 ile düşük, ardından %9,3 ile preterm doğum saptanmıştır. Düşüke etki eden faktörler araştırıldığında; CRL boyutunun hematomun yatay uzunluğuna oranına göre hesaplandığında ½ den fazla olanlarda ½ den az olanlara göre gebeliğin düşük ile sonuçlanmayıp gebeliğin devam etme olasılığını 2,923 kat artırmaktadır ve istatistiksel olarak anlamlı olduğu saptanmıştır. Düşük açısından, hematomun lokalizasyonu ve alanı arasında anlamlı bir fark tespit edilmedi. Subkoryonik hematomu olan gebelerde komplikasyona etki eden faktörler araştırıldığında; CRL boyutu ile hematomun yatay uzunluğunun oranının ½ den fazla olmasının ½ den az olmasına göre sağlıklı gebelik oluşma ihtimalini 2,325 kat artırdığı, CRL ile hematomun derinlik (longitudunal ) boyutuna oranının 1 den az olmasına göre 3-4 arasında olması sağlıklı gebelik oranını 3,439 kat oranında artırdığı ve 4 ten fazla olmasının ise 2,388 kat artırdığı saptanmıştır ve istatistiksel olarak anlamlı olduğu tespit edilmiştir. Sonuç: Çalışma sonucunda, gruplar arasında kötü obstetrik sonuç açısından anlamlı fark saptanmıştır. CRL boyutunun hematom boyutu ile oranlandığı hasta gruplarında düşük ve komplikasyon sonuçlarını öngörmede istatistiksel olarak anlamlı etkinliği olduğu gösterilmiştir.

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