Sürdürülebilir Kalkınma Amaçları
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- ArticleAçık Erişim2026Chemical Characterization and Antimicrobial Activity of Pyrolysis Liquids from Walnut Residue
Koc, Ibrahim; Ogun, Erdal; Geven, Fatmagul; Guney, Kerim; Yildiz, Faruk; Kaya, Ozkan
Pyrolysis liquid (PL) derived from biomass pyrolysis exhibits biopesticidal properties and represents a promising value-added product within the sustainable circular economy framework. However, knowledge about the antimicrobial potential of PLs produced from walnut residue at different pyrolysis temperatures remains limited. We investigated the chemical composition and antimicrobial activity of PLs obtained from agricultural walnut residue (Juglans regia L.) against selected plant pathogenic bacteria and fungi. PLs were produced at four temperature ranges: 200–300 °C (W-1), 300–400 °C (W-2), 400–500 °C (W-3), and 500–600 °C (W-4). Chemical characterization was performed using Gas chromatography–mass spectrometry (GC-MS), High-performance liquid chromatography (HPLC), and Inductively coupled plasma optical emission spectrometry (ICP-OES), with determination of total phenolic and flavonoid contents. Pyrolysis temperature significantly influenced the chemical profile and bioactive compound content of the PLs, with W-4 showing the highest total phenolic and flavonoid levels. Heavy metal analysis indicated minimal contamination in all samples. Antibacterial activity was observed in stock solutions, whereas diluted applications showed limited effects. The W-4 fraction showed the strongest antibacterial activity and exhibited MIC values of 12.50 µL/mL against Clavibacter michiganensis subsp. michiganensis, Xanthomonas euvesicatoria, and Pseudomonas syringae pv. syringae, and 25.00 µL/mL against Erwinia amylovora. Antifungal activity differed markedly across temperature ranges, with W-3 and W-4 displaying superior activity against Fusarium oxysporum and Verticillium dahliae, achieving complete mycelial growth inhibition at 5%, compared to 10% for W-2 and 20% for W-1. Positive controls confirmed assay validity (ciprofloxacin for antibacterial assays and cycloheximide for antifungal assays), whereas negative controls showed no inhibitory effect. Overall, higher pyrolysis temperatures, particularly 400–600 °C, enhanced the antimicrobial potential of walnut residue-derived PLs, supporting their possible use as bio-based antifungal agents for sustainable crop protection.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2026Divergent Biochemical Strategies and Organ-Specific Metabolic Adjustments in Spinach Mediated by Exogenous Amino Acids Under Salt Stress
Turfan, Nezahat; Tekşen, Kübra; Altuner, Ergin Murat; Yıldız, Faruk; Kaya, Ozkan
Understanding the physiological and biochemical responses of spinach to salt stress through amino acid supplementation is crucial for improving crop resilience under increasing soil salinity conditions. Salt stress represents one of the most severe abiotic constraints limiting vegetable crop productivity worldwide, yet comprehensive studies examining organ-specific metabolic reprogramming across entire plant systems remain limited. However, knowledge about how different amino acids mediate these responses through distinct metabolic pathways is limited. We investigated mineral nutrition, antioxidant defense systems, and secondary metabolite profiles of spinach supplemented with three amino acids (asparagine, phenylalanine, and tryptophan) under salt stress conditions. Amino acid type, salt treatment, and organ significantly influenced all measured parameters (p ≤ 0.0001). Asparagine consistently demonstrated comprehensive protective effects, excluding toxic ions while promoting calcium uptake, maintaining photosynthetic capacity, and dramatically enhancing flavonol biosynthesis (quercetin and rutin accumulation increased several-fold) under combined stress compared to other treatments. Phenylalanine excelled in ionic homeostasis restoration, achieving superior Na/K ratio reduction and enhanced phenylpropanoid pathway activation through elevated cinnamic acid biosynthesis. Tryptophan uniquely triggered exceptional divalent cation accumulation (6–10-fold increases in magnesium, calcium, and phosphorus) and maximally enhanced antioxidant enzyme activities, though with notable protein synthesis trade-offs. Organ-specific accumulation patterns revealed leaves as primary sites for photosynthetic pigments and phenolic compounds, roots as storage organs for specialized flavonoids and catechins, and petioles showing exceptional rutin accumulation. These findings demonstrate that amino acid selection fundamentally reshapes metabolic priorities in salt-stressed spinach through divergent yet complementary biochemical strategies. We conclude that amino acid selection significantly influences spinach resilience to salt stress through divergent metabolic reprogramming strategies, with each amino acid offering distinct advantages depending on cultivation priorities. Differential metabolic responses between amino acids provide insights for precision agriculture applications, while the quantitative biochemical patterns identified offer valuable parameters for optimizing amino acid supplementation strategies under saline conditions.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2025Exploring the Complete Chloroplast Genomes of Key Lamiaceae Species Uncovers the Secrets of Evolutionary Dynamics and Phylogenetic Relationships
Alp Furan, Mehmet; Yildiz, Faruk; Kaya, Ozkan
Chloroplast genome analysis provides crucial insights into plant evolution, classification, and conservation strategies. This study aimed to conduct a comprehensive comparative analysis of chloroplast genome architecture, gene content, and evolutionary relationships among five species of Lamiaceae (Lavandula angustifolia, Mentha × piperita, Ocimum × africanum, Salvia japonica, and Thymus serpyllum). Complete chloroplast genome sequences were retrieved from the NCBI database and analyzed using a systematic bioinformatics pipeline. Genome annotation was performed using Geneious Prime software, while repetitive sequence analysis was conducted using Tandem Repeats Finder. Phylogenetic relationships were reconstructed using MEGA software, implementing both the DualBrothers model and Neighbor-Joining method. The analyzed genomes exhibited the characteristic quadripartite structure, with sizes ranging from 152,048 to 153,995 base pairs and GC content between 37.8 and 38.0%. Each genome contained 131–134 genes, including 50 protein-coding sequences, 8 rRNA genes, and 37 tRNA genes. Comparative analysis revealed region-specific GC content variations, with IR regions showing the highest (43.0–43.4%), followed by LSC (35.9–36.2%) and SSC regions (31.6–32.1%). Codon usage analysis demonstrated a significant bias toward T/C-ending codons, particularly TTT, AAA, and AAT, correlating with the high AT content. Notable variations were observed at the LSC/IR/SSC junction regions, attributed to IR expansion and contraction. Molecular clock analyses indicated consistent evolutionary rates across the studied species. These findings provide valuable insights into the molecular evolution of Lamiaceae chloroplast genomes and establish a foundation for future research in plant molecular biology, systematic studies, and conservation efforts.
RDL Kalıcı Kaydıİncele - CorrectionAçık Erişim2025Correction to: Essential oils combat Botrytis cinerea in apples: unveiling sugar, vitamin, and antioxidant dynamics (Journal of Food Measurement and Characterization, (2024), 18, 7, (5590-5601), 10.1007/s11694-024-02590-y)
Karakus, Sinem; Atıcı, Okkes; Turan, Metin; Kaya, Ozkan
In this article, the second reference ‘A. Muder, H. Garming, S. DreisiebnerLanz, K. Kerngast, F. Rosner, K. Klickova, G. Kurthy, K. Cimer, A. Bertazzoli, V. Altamura, G. De Ros, K.M.B.C. Zmarlicki Freitas, A. Duarte, E. Bravin, J. Kambor, D. Karamürsel, F.P. Öztürk, E. Kaçal, Apple production and apple value chains in Europe. Eur. J. Hortic. Sci. 1611–4434 (2022)’ was incorrectly published and should have been ‘A. Muder, H. Garming, S. DreisiebnerLanz, K. Kerngast, F. Rosner, K. Klickova, G. Kurthy, K. Cimer, A. Bertazzoli, V. Altamura, G. De Ros, K.M.B.C. Zmarlicki Freitas, A. Duarte, E. Bravin, J. Kambor, D. Karamürsel, F.P. Öztürk, E. Kaçal, Apple production and apple value chains in Europe. Eur. J. Hortic. Sci. 87(6), 1–22 (2022). https://doi.org/10.17660/ejhs.2022/059’. The original article has been corrected.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2025Revealing the Environmental Factors That Influence the Leaf Biochemistry and Total Antioxidant Activity of Prunus laurocerasus L.
Turfan, Nezahat; Kara, Ferhat; Yıldız, Faruk; Güney, Kerim; Kaya, Ozkan
Understanding the seasonal dynamics of phytochemical composition in evergreen species is crucial for improving ecosystem productivity models and selecting appropriate species for urban landscapes under changing climate conditions. However, knowledge about how light environment, temperature, and precipitation interact to regulate leaf biochemical processes across seasons remains limited. We investigated morphological and biochemical responses of cherry laurel (Prunus laurocerasus L.) grown under contrasting light environments (light-exposed versus shaded) across twelve months, analyzing photosynthetic pigments, antioxidants, osmolytes, and secondary metabolites in relation to environmental variables. Light-exposed leaves exhibited enhanced accumulation of photoprotective compounds, including carotenoids (9.38 mg g−1), xanthophylls (3.60 mg g−1), and flavonoids (0.51 mg g−1), along with superior total antioxidant capacity during spring and autumn. Proline showed bimodal seasonal peaks (93.7 µmol g−1 in August under shade, 71.1 µmol g−1 in July under light), indicating stress responses to both summer heat and winter cold. Multivariate analyses revealed that seasonal variation accounted for 94.9% of total phytochemical variability, with distinct metabolic signatures characterizing winter (high glycine betaine, anthocyanin), spring (high chlorophyll, phenolics), summer (high proline, transient carotenoid peaks), and autumn (maximum antioxidant capacity) periods. We conclude that light environment significantly influences cherry laurel’s seasonal metabolic strategies, with shade-grown plants prioritizing light harvesting efficiency and osmotic adjustment, while light-exposed plants emphasize photoprotection and antioxidant defense. The coordinated regulation of functionally related compounds reveals integrated stress response mechanisms that contribute to cherry laurel’s remarkable environmental plasticity. These quantitative seasonal patterns provide valuable parameters for optimizing cultivation practices, predicting biochemical composition for harvesting purposes, and modeling the ecological performance of this species in variable urban and forest environments under climate change scenarios.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2025Modulation of Biochemical Traits in Cold-Stored ‘Karaerik’ Grapes by Different Edible Coatings
Keskin, Nurhan; Karakus, Sinem; Hatterman-Valenti, Harlene; Kaya, Ozkan; Cavusoglu, Seyda; Tekin, Onur; Kunter, Birhan; Keskin, Sıddık; Kaya, Ahmet Çağlar; Karadogan, Birol
Understanding the effects of edible coatings on postharvest quality and shelf life of ‘Karaerik’ grapes is crucial for improving storage outcomes and reducing losses. However, limited information exists regarding the effectiveness of different coating materials on this regionally significant variety. In this study, ‘Karaerik’ grapes were treated with carboxymethyl cellulose (CMC) and locust bean gum (KB) coatings and stored under cold conditions (0 ± 0.5 °C, 90–95% relative humidity) for 0, 25, 45, and 60 days. Storage duration and coating treatments significantly affected most physical, physiological, and biochemical parameters. During storage, grape weight loss progressively increased, reaching 9.60% in the control by day 60. Coatings slightly reduced this loss, with KB showing the lowest (5.11%) compared to the control (5.69%). Respiration initially declined but surged again at day 60, especially in the control (96.4 μmol CO2/kg·hour), while coatings helped mitigate this rise. Ethylene release remained unchanged. A slight pH decline (~4.6%) was observed in the control, while KB-treated grapes maintained higher pH and lower acidity. Soluble solids remained stable across treatments. Color changed notably during storage: a* nearly doubled (more redness), b* increased (less blue), and chroma (C*) declined by ~25%, especially in uncoated grapes. Total sugar dropped by ~43% in KB-treated grapes, with the control retaining the most. Tartaric acid decreased by ~55%, notably in KB samples. Antioxidant activity and total phenolics declined significantly (~66%) in the control. CMC coating better-preserved antioxidant capacity, while the control showed the highest phenolic levels overall. Ferulic, gallic, and chlorogenic acids increased toward the end of storage, particularly in coated grapes. In contrast, rutin and vanillic acid peaked mid-storage and were better preserved in the control. The heatmap showed significant metabolite changes in fruit samples across 0D, 25D, 45D, and 60D storage periods under CMC, CNT, and KB treatments, with distinct clustering patterns revealing treatment-specific biochemical responses. The correlation matrix revealed strong positive relationships (r > 0.70) between total sugar, glucose, and fructose levels, while ethylene showed significant negative correlations (−0.65 to −0.85) with maturity index, pH, and total soluble solids, indicating interconnected metabolic pathways during fruit ripening and storage. We conclude that edible coating selection significantly influences grape biochemical stability during cold storage, with CMC emerging as a superior choice for maintaining certain quality parameters.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2025Timing Matters, Not Just the Treatment: Phenological-Stage-Specific Effects of Seaweed and Ethanol Applications on Postharvest Quality of ‘Tarsus Beyazı’ Grapes
Tarım, Güzin; Karakus, Sinem; Keskin, Nurhan; Hatterman-Valenti, Harlene; Kaya, Ozkan
In the context of increasing consumer demand for high-quality, residue-free fruits and the growing emphasis on sustainable postharvest technologies, identifying effective, eco-friendly treatments to maintain grape quality during storage has become a critical focus in modern viticulture. Over the course of this study, we examined the influence of seaweed extract (derived from Ascophyllum nodosum) and ethanol-based postharvest treatments on the postharvest quality of the ‘Tarsus Beyazı’ grape. The seaweed extract was applied at six specific phenological stages according to the BBCH scale: BBCH 13 (3rd–4th leaf stage, 0.40%), BBCH 60 (first flower sheath opening, 0.50%), BBCH 71 (fruit set, 0.50%), BBCH 75 (chickpea-sized berries, 0.50%), BBCH 81 (start of ripening, 0.60%), and BBCH 89 (harvest maturity, 0.60%). After harvest, grape clusters were subjected to four different postharvest treatments: untreated control, control + ethanol (20% ethanol immersion for 10 s), seaweed extract alone (preharvest applications only), and seaweed extract + ethanol (combining both preharvest and postharvest treatments). Grapes were stored at 0–1 °C and 90–95% RH for three weeks, followed by a shelf-life evaluation period of three days at 20 °C and 60–65% RH. The findings revealed that seaweed treatments, especially when applied during cluster formation and berry development, effectively mitigated physiological deterioration, preserving stem turgidity and enhancing berry firmness. In contrast, ethanol showed variable responses, occasionally exerting negative effects, with only marginal benefits observed when applied at optimal developmental stages. Both the type and timing of application emerged as critical determinants of key quality attributes such as weight loss, decay incidence, and must properties (TSS, pH, TA). Correlation and heat map analyses indicated the interrelationships among these parameters and the differential impacts of treatments. These results suggest that phenological-stage-specific seaweed applications hold significant potential as a sustainable strategy to extend the storage life and maintain the market quality of ‘Tarsus Beyazı’ grapes.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Evaluation of coeliac trunk and hepatic artery variations in thoracic CT angiography in patients with congenital heart disease
Memis, Kemal Bugra; Bulut, Hasan; Cinar, Hasibe Gokce; Ucan, Berna; Uner, Cigdem; Kaya, Ozkan; Aydin, Sonay
Introduction: Understanding the variations of abdominal vascular structures is important for preventing complications of abdominal surgical procedures for gastrointestinal disease such as necrotizing enterocolitis or others that may arise in patients with congenital cardiac disease. We analysed the coeliac trunk and its branches in children with congenital heart disease to determine whether there is a greater prevalence of associated vascular abnormalities. Methods: We retrospectively analysed thoracic computed tomography (CT) angiograms performed in our hospital in paediatric patients with congenital heart disease. We documented the anatomical variations observed in abdominal sections in which the coeliac trunk and hepatic arteries were included in the field of view. We used the Uflacker classification to describe anatomical variants of the coeliac trunk, and the Michels classification and its modified version (Hiatt classification) to describe the anatomy of the hepatic artery system. Results: Our study included 178 patients with congenital heart disease. We identified coeliac trunk variants in 10.7% of the patients. Gastrosplenic trunk was to the most prevalent variant, amounting to 5.6% of total cases. We found hepatic artery variations in 19.1% of the patients. According to the Michels classification, the prevalence of accessory left hepatic artery arising from the left gastric artery as 4.5%, compared to 6.7% based on the Hiatt classification. Conclusion: The prevalence of coeliac trunk and hepatic artery variations in patients with congenital heart disease was not greater in our study compared to other series in the literature. Clinicians must be vigilant about the variations detected in multislice CT scans to avoid complications resulting from vascular abnormalities, especially in patients who undergo abdominal surgery. (c) 2024 Asociacion Espanola de Pediatr & imath;a. Published by Elsevier Espana, S.L.U. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/ 4.0/).
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Essential oils combat Botrytis cinerea in apples: unveiling sugar, vitamin, and antioxidant dynamics
Karakus, Sinem; Atıcı, Okkes; Turan, Metin; Kaya, Ozkan
This study investigated the effects of individual and combination applications of EOCs against Botrytis Cinerea in apple fruits, with a focus on sugar, vitamin, and antioxidant enzyme dynamics. In protective and curative treatments, sucrose content varied between 18.9 and 87.9 g.L-1, with the “CT” group exhibiting significantly higher sucrose levels (87.9 g.L-1). Glucose content ranged from 6.2 g.L-1 (“CT” and “C + F”) to 35.5 g.L-1 (“T + C + E + F”). Fructose content varied significantly, with the “F + T + C + E” treatment showing the highest (11.4 g.L-1) and the “CT” group the lowest (2.5 g.L-1). Rhamnose content in the curative treatment reached 14.9 g.L-1 (“F + T”) and in the protective treatment, the “C + E + F” group had the highest (3.3 g.L-1). Galactose content ranged from 0.1 to 13.0 g.L-1. Xylose content in curative treatment ranged from 12.1 to 13.6 g.L-1, with the combination “F + T” displaying the highest. In preventive treatment, arabinose content ranged from 0.7 to 3.0 g.L-1, with the “T + C + F” group having the highest. In terms of vitamins, the combination “T + F” showed the highest B1 (25.4 mg.100 g-1) and B2 (24.6 mg.100 g-1) content. Vitamin A, B1, and B2 increased in the “F + T” group, and vitamin C increased in the “F + T + C” group (33.4 mg.100 g-1). The quantification of oxidative stress markers and antioxidant enzymes revealed significant reductions in H2O2, MDA, and proline levels in EOC applications. In preventive treatment, the combination “T + C + E + F” exhibited the most effective improvement in SOD activity (556.5 EU mg-1), CAT activity increased significantly in the “T + C + F” group (189.3 EU mg-1), and APX activity saw the highest increase in the “T + C + E + F” group (185.6 EU mg-1). Contrastingly, curative treatments showed higher H2O2, MDA, and proline levels. The most significant decrease in H2O2 level occurred in the “T + C + E + F” group (36.7 nmol.g-1), and a significant decrease in MDA level was noted in the same group. Notable changes were observed in the activities of antioxidant enzymes, with the “F + T + C + E” group displaying the highest SOD activity (440.7 EU mg-1), and the “F + T” group showing the highest GST activity (542.0 EU mg-1). This research provided comprehensive insights into the biochemical responses of apple fruits to EOC treatments, offering potential strategies for effective fungal management in agriculture.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Land Cover Dynamics and Environmental Implications in Türkiye’s Riparian Zones: A Comprehensive Seven-Year Study
Akturk, Emre; Guney, Kerim; Aydin, Mirac; Yildiz, Faruk; Kaya, Ozkan
This study examines the Riparian Buffer Zones (RBZs) in Türkiye, analyzing land cover changes from 2017 to 2023 to assess their effectiveness in ecological conservation and water resource management. The research employs Esri-Sentinel land cover data, Shuttle Radar Topography Mission digital elevation models, and RiverATLAS data to identify and delineate RBZs, classify land cover types, and evaluate trends and changes over seven years. The findings indicate that 44.8% of RBZs are covered by ‘Rangelands’, 32.2% by ‘Crops’, and 16.1% by ‘Trees’ classes. The study notes a considerable increase in built areas and agricultural fields, which may indicate ongoing urban expansion and its potential impact on the effectiveness of RBZs. Despite regulatory efforts, the substantial presence of agriculture within RBZs indicates a need for more robust environmental protection policies. The results indicate a pressing need for a policy reassessment to enhance forest cover within RBZs and reduce agricultural encroachment. This would improve water quality and facilitate the achievement of sustainability goals numbered 2, 3, 6, 11, 14 and 15. The findings thus call for a strategic enhancement of RBZ management to better protect water resources against the adverse effects of agricultural and urban activities.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2024Essential oils in post-harvest disease management: Metabolic impact on Narince (Vitis vinifera L. cv) grapes against Botrytis cinerea
Kaya, Ozkan; Bozkurt, Abdurrahim; Karakus, Sinem; Daler, Selda; Yilmaz, Turhan; Turan, Metin
There is an urgent need for alternative, environmentally friendly post-harvest disease management solutions because of the growing concern over the use of chemical fungicides in agriculture and their detrimental impact on human health and the environment. This study examines the effects of thymol, eugenol, and 1,8-cineole essential oils on the sugar, organic acid, and amino acid profiles of 'Narince' grape berries as well as their effectiveness against gray mold (Botrytis cinerea). Our study has noted that the application of EOs, particularly eugenol, significantly alters the metabolic profile of 'Narince' grapes, enhancing sugar content and modifying the levels of organic acids and amino acids, which suggests a potential for improving grape quality and resistance to Botrytis cinerea. The combined use of eugenol with other EOs and fungicides, as indicated by the treatments OF and OSF, not only displayed a synergistic effect in elevating the concentration of key metabolites but also appeared to be more effective in combating fungal infection compared to individual EO applications. The PCA analysis and subsequent heatmap visualization revealed distinct metabolic clustering among the treatments, with combined EO treatments, especially those including eugenol, markedly influencing the amino acid profile of the berries. Our investigation's results suggest that EOs may offer a viable, eco-friendly alternative to traditional fungicides. Our study also adds to the body of knowledge supporting the advancement of integrated pest management strategies that are efficacious yet environmentally considerate, aligning with the global shift towards more sustainable practices in agriculture and food security.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2024Improving Biogenic Amines and Combating Botrytis cinerea Decay in ‘Golden Delicious’ Apples With Post-Harvest Essential Oil Treatments
Karakuş, Sinem; Kaya, Ozkan; Şahin, Müge
Malus Domestica Borkhausen, commonly known as ‘Golden Delicious’ apples, are highly favored for their nutritional attributes, including crisp texture, robust flavor, sweetness, firmness, color, and a rich array of phytochemical compounds, predominantly polyphenols. Despite their popularity, apples have a limited shelf life, particularly when stored at room temperature. EOs have emerged as a secure postharvest technology with the potential to mitigate post-harvest losses and preserve both labile constituents and overall quality. This study sought to assess the impact of EOs treatments on BAs within ‘Golden Delicious’ apple cultivar and their efficacy in combatting Botrytis cinerea during both protective and curative phases. Interestingly, a strong positive interplay was observed among Cad, His, and Try, while these BAs exhibited a negative association with Dop, Tma, and Nor. EOs treatments exhibited a general tendency to augment the levels of BAs during both preventive and curative periods. Put, Cad, His, and Try were identified as the predominant BAs in ‘Golden Delicious’ apple cultivar during the preventive and curative phases. The interrelation between EOs treatments and application timelines was found to be significantly associated with the levels of BAs, particularly involving combinations such as Eug + Fun, Cin + Fun, Thy + Eug + Fun, Thy + Cin + Fun, Thy + Cin + Eug + Fun, and curative phases. The outcomes of the investigation revealed that the combination of Fun + Thy + Cin + Eug treatments during the protective and curative phases was notably effective in reducing the incidence of fruit decay attributed to B. cinerea. Conversely, the study established correlations with terms such as Fungus, Cin + Eug + Fun, Thy + Fun, control, and protective. In conclusion, the research indicates the dual functionality of EOs application, both as a preventive measure against pathogenic agents such as B. cinerea and as a regulator of potentially harmful BAs within the apples. These findings hold promise for improving the quality and safety of ‘Golden Delicious’ apples in the marketplace, addressing both shelf life and consumer health considerations.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2024Preserving the richness of nature: cultural and ecological importance of edible wild plants in Sivas
Kadioglu, Zakine; Yildiz, Faruk; Kandemir, Ali; Cukadar, Kemal; Kalkan, Nalan Nazan; Vurgun, Huseyin; Donderalp, Veysel; Korkut, Recep; Kaya, Ozkan
This comprehensive article offers a thorough exploration of the abundant plant species that hold a deep connection with the history and daily life of Sivas, within the context of its traditional culture and ethnobotany. The Asteraceae family, which encompassed 22 species, emerged as the most diverse family. Other notable families included Brassicaceae (8 species), Fabaceae (9 species), Polygonaceae (10 species), Apiaceae (11 species), and Lamiaceae (21 species), and the study systematically classified 128 plant species and subplants into 29 different families. The study categorized these plants based on their edible parts to understand their role in Sivas’ culinary traditions, revealing a wide range of edible components, including leaves, seeds, flowers, roots, tubers, gum, fruits, branches, and more. Cerinthe minor, Berberis vulgaris, Stachys lavandulifolia and others provided nourishing leaves and young shoots. The study categorized plants based on their preparation methods, highlighting their integral role in Sivas’ traditional cuisine. Seeds namely Echinops orientalis and Cephalaria procera introduced culinary diversity, while certain plants, such as Geranium tuberosum and Cirsium rhizocephalum, contributed edible roots, expanding the repertoire of local dishes. Whether utilized in cooked dishes, salads, or as spices, these plants impart unique flavors and aromas to the local culinary creations. Whether used in cooked dishes, salads, or as spices, these plants add unique flavors and aromas to local food. Spices such as Mentha longifolia and Thymus leucotrichus enriched the culinary landscape, while Thymus sipyleus and Ziziphora clinopodioides brought their distinct tastes to dishes. The repeated use of common names across different species, such as “Yemlik” “Yağlıca” “Nane” and “Kekik” suggests a shared cultural heritage and linguistic connection among these plants, further emphasizing their significance in the local context. Five species have been newly documented as additions to the flora of Sivas. In summary, this study underscores the importance of preserving and appreciating the botanical knowledge and practices of Sivas traditional culture and ethnobotany. The numerical values within the findings quantitatively showcase the ecological and cultural richness of these plant species, thus making this subject a vital area of study for researchers and a valuable resource for conservationists aiming to safeguard this unique cultural and botanical heritage.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Physico-Chemical Responses of Alstroemeria spp. cv. Rebecca to the presence of Salicylic Acid and Sucrose in vase solution during postharvest life
Seyed Hajizadeh, Hanifeh; Bayrami Aghdam, Somayeh; Fakhrghazi, Hadi; Karakus, Sinem; Kaya, Ozkan
Background: The primary challenge in the cut flower industry, specifically in the postharvest phase, is the short vase life of flowers. This issue, along with early leaf yellowing and perianth abscission, significantly diminishes the economic value of flowers due to their accelerated senescence. To tackle this, we conducted a factorial experiment on Alstroemeria cv. Rebecca, utilizing a completely randomized design with three replications. In this experiment the effects of varying concentrations of Salicylic acid (SA) (0, 1.5, and 3 mM) and sucrose (SU) (0% and 3%) were investigated on the postharvest quality of leaves and florets, with systematic evaluations every three days throughout their vase life. Results: This experiment revealed that the specific treatment combination of 1.5 mM SA + 3% SU (T5) markedly improved various parameters, such as vase life, total chlorophyll content, membrane stability index, relative fresh weight, and water uptake of cut flowers. In our analysis, we observed that this preservative solution not only extended the vase life and enhanced water uptake but also effectively preserved total chlorophyll, mitigated the loss of fresh weight, and reduced membrane deterioration in petals. Additionally, our results showed an increase in the activities of catalase (CAT) and peroxidase (POD) enzymes, as well as total protein content, alongside a decrease in malondialdehyde (MDA) and hydrogen peroxide (H2O2) levels. Moreover, this study noted a decrease in microbial populations in solutions containing different concentrations of salicylic acid. Conclusions: Our research demonstrated that alstroemeria flowers maintained in a solution with 1.5 mM SA + 3% SU exhibited a significantly prolonged vase life of up to 21 days, in contrast to the 15 days observed in control flowers kept in water. These results are highly beneficial for manufacturers in the cut flower industry, as they provide a viable method to substantially extend the vase life of cut flowers. Such an enhancement in flower longevity can lead to increased market value and customer satisfaction. Furthermore, the reduction in flower senescence and decay rates can contribute to decreased waste and greater efficiency in cut flower distribution and sales, offering a substantial advantage to manufacturers in this competitive market. The extended vase life and reduced senescence observed in alstroemeria flowers treated with 1.5 mM SA and 3% SU are attributed to SA's role in enhancing endogenous defense responses and sucrose's function as an energy source, collectively improving water uptake, and delaying the natural decay process.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Pollination Strategies and Reproductive Biology of Fritillaria imperialis L. (Liliaceae): Insights from Erzincan, Türkiye
Yildiz, Faruk; Aslay, Meral; Kaya, Ozkan
Fritillaria spp., comprising perennial bulbous plants of significant medicinal and ornamental value, face high endangerment in their natural habitats. Despite their importance, the reproductive characteristics and adaptive evolution mechanisms of these species remain incompletely understood. This study focused on the pollination strategies and reproductive biology of Fritillaria imperialis L. The research was conducted at the Erzincan Horticultural Research Institute in Türkiye. Our investigation categorized the flowering process of F. imperialis into nine distinct phases. Through comprehensive assessments of the pollen/ovule ratio, self-incompatibility index (SII), and ex situ pollination experiments, we observed high levels of self-incompatibility and allogamy in F. imperialis. Our findings revealed that pollination of F. imperialis primarily relied on pollen vectors, with Apis mellifera and Bombus terrestris identified as the most effective pollinators. Furthermore, average seed set rate, seed production, and seed viability were quantified at 80.5%, 228 seeds, and 86.3%, respectively. The average numbers of pollen viability and pollen grains were measured at 93% and 702,000, respectively. This comprehensive analysis of the reproductive biology of F. imperialis provides crucial insights for the conservation and genetic management of this highly valuable species. The findings contribute to a deeper understanding of the reproductive strategies employed by Fritillaria spp., which may inform future conservation efforts and breeding programs for these endangered plants.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2024Essential oil compounds modulate nutritional quality and stress response in Botrytis cinerea-infected grape (Vitis vinifera L. cv. ‘Karaerik’)
Kaya, Ozkan; Bozkurt, Abdurrahim; Karakus, Sinem; Daler, Selda; Yilmaz, Turhan
Grapes, rich in vitamins and minerals, could offer a healthy alternative. However, their potential contamination by the fungus Botrytis cinerea poses serious health risks, necessitating cautious consumption, particularly among those on restricted diets, to mitigate adverse effects. Therefore, the study investigated the effects of individual and combined application of individual components of essential oils (EOs) such as thymol, eugenol, and 1,8-cineole on various parameters such as antioxidant enzymes, mineral elements, anthocyanins, and phenolic compounds in the treatment of B. cinerea infection in 'Karaerik' grapes. Our study provided important information about physiology and pathogen response, and results indicated that the pathogen-infected groups had higher levels of oxidative stress indicators, such as proline, malondialdehyde (MDA), and hydrogen peroxide (H2O2). On the other hand, EOs elevate the activity of antioxidant enzymes, notably superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), whereas thymol treatments effectively reduced oxidative stress to levels comparable to the control group. Under thymol and eugenol treatments, nitrogen (N), potassium (K), phosphorus (P), calcium (Ca), magnesium (Mg), sulfur (S), zinc (Zn), manganese (Mn), boron were significantly higher than the control groups. Increases were also determined in the concentrations of mineral elements such as (B) and copper (Cu). Anthocyanin profiles were preserved by EO treatments, with combined EO applications yielding higher anthocyanin profiles percentages compared to individual treatments. In our results, there were notable differences in the overall quantities of phenolic compounds, and the combination of EO applications caused higher levels of phenolic compounds, especially in the groups that include the fungal infection. Overall, the study showed how effective EO treatments are at reducing B. cinerea infections and maintaining grape quality, which has significance for long-term disease control plans and maintaining berry quality in viticulture.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024When Undergoing Thoracic CT (Computerized Tomography) Angiographies for Congenital Heart Diseases, Is It Possible to Identify Coronary Artery Anomalies?
Uner, Cigdem; Gulmez, Ali Osman; Cinar, Hasibe Gokce; Bulut, Hasan; Kaya, Ozkan; Gokharman, Fatma Dilek; Aydin, Sonay
Introduction and Objective: The aim of this study was to evaluate the coronary arteries in patients undergoing thoracic CT angiography for congenital heart disease, to determine the frequency of detection of coronary artery anomalies in congenital heart diseases, and to determine which type of anomaly is more common in which disease. Materials and Methods: In our investigation, a 128-detector multidetector computed tomography machine was used to perform thorax CT angiography. The acquisition parameters were set to 80–100 kVp based on the patient’s age and mAs that the device automatically determined based on the patient’s weight. During the examination, an intravenous (IV) nonionic contrast material dose of 1–1.5 mL/kg was employed. An automated injector was used to inject contrast material at a rate of 1.5–2 mL/s. In the axial plane, 2.5 mm sections were extracted, and they were rebuilt with 0.625 mm section thickness. Results: Between October 2022 and May 2024, 132 patients who were diagnosed with congenital heart disease by echocardiography and underwent Thorax CT angiography in our department were retrospectively evaluated. Of the evaluated patients, 32 were excluded with exclusion criteria such as patients being younger than 3 months, older than 18 years, insufficient contrast enhancement in imaging and contrast-enhanced imaging, thin vascular structure, and motion and contrast artifacts; the remaining 100 patients were included in this study. The age range of these patients was 3 months to 18 years (mean age 4.4 years). Conclusion: In congenital heart diseases, attention to the coronary arteries on thoracic CT angiography examination in the presence of possible coronary anomalies may provide useful information.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Understanding the impact of essential oils on grape metabolism and pathogen resistance: a study with a focus on Botrytis cinerea
Kaya, Ozkan; Karakus, Sinem; Bozkurt, Abdurrahim; Yilmaz, Turhan; Hajizadeh, Hanifeh Seyed; Turan, Metin
Background: The exploration of EOs and their impact on primary metabolites in agricultural products, such as grapes, holds significant importance in the post-harvest preservation and enhancement of berry quality. Therefore, our research aimed to dissect the effects of various EO treatments on the biochemical composition of grapes, specifically examining alterations in sugar, organic acid, and amino acid levels. Results: Our findings highlighted that the OF treatment, a combination of Eugenol and Botrytis cinerea, significantly escalated sugar content, with remarkable increases in sucrose, glucose, and fructose compared to the control group. The SF treatment led to the highest levels of certain organic acids, including tartaric, butyric, and malonic acids, whereas the TO treatment prominently augmented amino acids such as tyrosine and phenylalanine. Utilizing PCA, our study effectively captured the variability within the treatment dataset, revealing distinct clusters of treatments and their correlations with specific metabolites. The PCA biplot underscored the influence of treatments on metabolic profile of grape berries, with treatments like OSF, TO, and TOSF demonstrating close associations with specific amino acids, suggesting positive correlations. Conclusion: Our current results indicate that EOs substantial influence on the metabolic profile of grapes, resulting in enhanced fruit quality and extended shelf life. Variations in sugar, organic acid, and amino acid levels among different EO treatments highlight their potential for improving post-harvest characteristics. To sum up, these findings are valuable for the agricultural and food industries, showcasing the utility of EOs as natural preservatives and enhancers in fruit cultivation and storage. Graphical Abstract: (Figure presented.)
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023Exploring the Biodiversity and Conservation Value of Alpine Grasslands in the Bucegi Massif, Romanian Carpathians
Bita-Nicolae, Claudia; Yildiz, Faruk; Kaya, Ozkan
The Carpathian region harbors a wide range of threatened species, making it an area of exceptional conservation value. In the Alpine belt, grasslands cover the entire region and this study aims to describe the communities in the Bucegi Massif of the Romanian Carpathians and highlight their importance for conservation. The Braun–Blanquet approach was used to record floristic data from 47 phytosociological surveys, identifying a total of 235 plant species from 40 different families, including 30 threatened species. Canonical correspondence analysis was used to analyze the data, revealing that the distribution of vegetation is mainly influenced by elevation, slope and vegetation cover. Dominant grass species in these communities include Nardus stricta, Festuca violacea, Kobresia myosuroides, Festuca amethystina, Festuca airoides, Sesleria rigida, Festuca versicolor and Festuca carpatica. The alpine and boreal siliceous grasslands of the Carpathian Mountains, identified by Natura 2000 codes 6150, 6130 and 6170, host a wide range of plant species of significant conservation value. The higher altitude grasslands, especially, have outstanding plant species richness. We argue that although the habitats have been grazed, significant parts of the area are still in good ecological condition, having many typical natural features.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2023Comparison of contents of sugars, organic acids and free amino acids in raisins obtained from Gök Üzüm (Vitis vinifera L.)
Karakus, Sinem; Ates, Fadime; Keskin, Nurhan; Turan, Metin; Kaya, Ozkan
Raisins are known as an important source of many bioactive compounds such as organic acids, free amino acids, and sugars. In this study, oak ash and potassium carbonate solutions applied before drying were compared for their effects on the Gök Üzüm raisin, more specifically, its contents of organic acids, free amino acids, and sugar profile. The total acidity quantified in the samples of raisin, ranged from 20.30 to 117.08 ng/µL DW for the potassium dipping solution, while values ranged from 17.98 to 164.72 ng/µL DW for the oak ash dipping solution. In samples from either dip solution, serine was predominant, followed by alanine, asparagine, glutamine, aspartate, sarcosine, glutamate, and leucine, whereas gly-cine, arginine, proline, histidine, and valine were found to be the lowest amino acids. In the samples examined, fructose was the most abundant sugar, whereas the lowest sugars in raisins were xylose for oak ash application and galactose for potassium carbonate application. There was wide variation in the sugar profiles for both variants, with values ranging from 3043.48 to 0.71 g/100 g DW. Drying Gök Üzüm grapes after the treatment of oak ash dipping solution promotes a higher content of organic acids, amino acids and sugars as compared to drying after potassium carbonate solution treatments. These results could be used for the development of an attractive pre-drying solution in further studies for the production of both, raisins and perhaps other dried fruits.
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