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- ArticleYalnız künye2022Molecular Mechanism and Pathogenesis-Oriented Treatment of Antipsychotic Drug-Induced Infertility in Female Rats
Ozyurt, Ramazan; Suleyman, Halis; Dinc, Kemal; Yildiz, Sevler; Coban, Taha Abdulkadir; Bulut, Seval; Suleyman, Zeynep; Altuner, Durdu; Keskin, Deha Denizhan; Keskin, Seda
Typical and atypical antipsychotics may cause subfertility through oxidative stress in reproductive cells and endocrine axis. This experimental study was designed to determine the effects of adenosine triphosphate (ATP) treatment on oxidative stress markers and hyperprolactinemia in subfertility due to antipsychotics. A total of 54 Albino Wistar female rats were divided into nine groups as the healthy (HG), haloperidol (HPL), olanzapine (OLZ), clozapine (CLZ), aripiprazole (ARZ), ATP+haloperidol (ATP+HPL), ATP+olanzapine(ATP+OLZ), ATP+clozapine (ATP+CLZ) and ATP+aripiprazole (ATP+ARZ) groups. ATP was injected intraperitoneally (ip) at a dose of 25 mg/kg to animals in ATP+HPL, ATP+OLZ, ATP+CLZ, and ATP+ARZ groups. HG, HPL, OLZ, CLZ, and ARZ groups were given the same volume of distilled water via ip. One hour after administration of drugs and solvent, haloperidol (2 mg/kg) for ATP+HPL and HPL groups, olanzapine (2 mg/kg) for ATP+OLZ and OLZ groups, clozapine (20 mg/kg) for ATP+CLZ and CLZ groups and aripiprazole (3 mg/kg) for ATP+ARZ and ARZ groups was given orally to by gavage. This procedure was repeated once a day for 15 days. After this period, blood samples were taken for oxidant, antioxidant and prolactin measurement and the animals were kept with mature male rats for one month for breeding. The degree of oxidative stress, hyperprolactinemia and subfertility caused by antipsychotics were found to be haloperidol, olanzapine, clozapine, aripiprazole, in order of frequency. ATP administration reversed the increase in oxidants markers and the decrease of antioxidants significantly. ATP administration prevents subfertility due to antipsychotic drugs.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2022The Effect of Cerebrolysin on Ischemia Reperfusion-Induced Oxidative and Inflammatory Ovarian Damage in Rats
Ozer, Mehmet; Ince, Sefa; Suleyman, Bahadir; Mammadov, Renad; Yavuzer, Bulent; Bulut, Seval; Akbas, Nergis; Mokhtare, Behzad; Mendil, Ali S.; Dinc, Kemal
SUMMARY. Ovarian ischemia-reperfusion (I/R) injury develops due to oxidative stress and inflammation that occurs when tissue is deprived of oxygen. Ovarian I/R damage also causes infertility. The aim of this study is to see the effect of ovarian I/R damage on reproductive functions and to determine the protection of antioxidant and anti-inflammatory cerebrolysin in possible damage. Albino Wistar-type female rats were divided into sham operation (SG), ischemia-reperfusion (IR) and cerebrolysin+ischemia-reperfusion (CIR) groups. After I/R application with cerebrolysin, the ovaries of six animals from each group were taken and biochemical and histopathological examinations were performed. Six animals from each remaining group were left to breed. Our test results showed that cerebrolysin decreased I/R-related oxidant, proinflammatory cytokine and tissue damage and increased antioxidant levels. In addition, cerebrolysis significantly inhibited the delay of the maternity period. These findings indicate that cerebrolysin may be helpful in the treatment of I/R-related ovarian damage and infertility.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2022The Effect of Adenosine Triphosphate, Benidipine, Sugammadex, and their Combinations upon Cardiac Damage after Temporary Clamping of the Abdominal Aorta in Rats
Emir, Izzet; Altuner, Durdu; Gulaboglu, Mine; Coban, Taha Abdulkadir; Suleyman, Bahadir; Mammadov, Renad; Ozkaraca, Mustafa; Suleyman, Zeynep; Karakurt, Tulay Ceren Olmezturk; Suleyman, Halis
The aim of our study was to biochemically and histopathologically investigate the protective effect of adenosine triphosphate (ATP), benidipine, sugammadex and (ATP + benidipine + sugammadex) ABS against cardiac damage induced by abdominal clamping and unclamping (ACU) procedure in rats. Rats utilized were divided into six groups as Healthy (HG), Abdominal ACU (AACC), ATP + AACU (ATPG), Benidipine + AACU (BNDG), Sugammadex + AACU (SDXG), ABS + AACU (ABSG). One hour before anesthesia, ATP (25 mg/kg; ip) was administered to the ATPG group, Benidipine (4 mg/kg orally) was administered to the BNDG group, Sugammadex (4 mg/kg ip) was administered to the SDXG and ABS was administered with this specified dose and method to the ABSG group. Distilled water as a solvent was injected to AACC and HG groups. One hour after, laparotomy was performed to the rats, and the abdominal cavity was reached. Ischemia was provided for one hour and then reperfusion for 2 h placing an atraumatic clamp on the suprarenal abdominal aorta of the animals in all groups (except from the HG). The heart tissues extracted from the killed animals were examined biochemically and histopathologically. ATP and benidipine suppressed the increase of oxidant and proinflammatory cytokines better than sugammadex. The ABSG group was found to be almost the same as the healthy group biochemically and histopathologically (p > 0.05). ABS drug form alone was possible to be much more beneficial rather than ATP, benidipine and sugammedex in the treatment of cardiac damage induced by AACU procedure.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2022Effect of Rhodiola rosea Root Extract on Stress-Induced Gastric Ulcer in Rats
Isik, Bahar; Altuner, Durdu; Ozcicek, Fatih; Ezmeci, Talat; Aggul, Ahmet G.; Suleyman, Bahadir; Bulut, Seval; Suleyman, Zeynep; Yavuzer, Bulent; Suleyman, Halis
Stress can lead to stomach ulcers. Oxidative stress and inflammation are involved in the pathogenesis of stress ulcers. Rhodiola rosea root extract (RRE) has antistress, anxiolytic, antioxidant and anti-inflammatory effects. In this study, the effect of RRE on gastric ulcers induced by forced immobilization method (FIM) was investigated. Albino Wistar male rats were divided into healthy (HG), control (SC), stress+RRE (SRE) and stress+famotidine (SFM) groups. RRE was given to the rats’ SRE group, and famotidine to the SFM group was administered orally. One hour after, the animals in the SRE, SFM and SC groups were subjected to stress with FIM for one hour once a day. This procedure continued for seven days. After this period, the animals were euthanized. Stomach tissues were examined macroscopically and biochemically. RRE reduced the number and area of stress-induced ulcers and biochemical changes in the gastric tissues of animals better than famotidine.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2022Effect of sunitinib on testicular oxidative and proinflammatory damage induced by ischemia–reperfusion in rats; [Efecto de sunitinib en el daño oxidativo y proinflamatorio testicular inducido por isquemia-reperfusión en ratas]
Keskin, Ercument; Erdogan, Abdullah; Suleyman, Halis; Yazici, Gulce Naz; Sunar, Mukadder; Gul, Mehmet Ali
Introduction: This study aimed to biochemically and histopathologically investigate the effect of sunitinib on oxidative testicular damage induced by ischemia/reperfusion in rats. Material-method: Experimental animals were divided into three groups of six rats each: testicular torsion–detorsion (TTD), sunitinib + testicular torsion–detorsion (STD), and sham control (SC). Sunitinib (25 mg/kg) was administered orally to the STD group by gavage. Normal saline (0.9% NaCl) was administered orally to the TTD and control groups as the solvent. One hour after administration of sunitinib and 0.9% NaCl, all animal groups were done torsion–detorsion. Then, all the rats were killed by high-dose anesthesia, and their testicles were removed. Biochemical and histopathological examinations were performed on the removed testicular tissues. Results: Malondialdehyde; it was observed that the results in the STD group were close to those of the SC group and statistically significant lower compared to the TTD group (p = 0.001). The glutathione values were statistically significantly higher in the STD group compared to the TTD group (p < 0.001). Nuclear factor kappa B values, revealing a statistically significant difference between the TTD and STD groups (p < 0.001). The TNF-α levels were measured and indicating that the results of the STD group were statistically significantly lower than those of the TTD group (p < 0.001). Histopathologically, animal tissues given sunitinib were observed to resemble normal tissues. Conclusion: Sunitinib was shown to prevent histopathological changes in testicular tissue against ischemia/reperfusion damage.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2022Prevention of the Paracetamol-Induced Liver Injury with Pycnogenol® in Rats
Topal, Ismail; Bilgin, Asli Ozbek; Çatakli, Tulin; Cimen, Ferda Keskin; Cankaya, Murat; Suleyman, Halis
This study aimed to evaluate the effects of pycnogenol®, an anti-oxidant, anti-inflammatory, and anti-cancer agent, on paracetamol-induced oxidative liver injury in rats. Albino Wistar rats were divided three groups of six rats: paracetamol (PAC), pycnogenol® and paracetamol (PPC), and healthy (HG) groups. Forty mg/kg pycnogenol (to PPC) or distilled water (to PAC, healthy group) was administered to the stomach of the rats by oral gavage. One hour before the medications 1000 mg/kg paracetamol was orally administered to the PPC and PAC groups. In PAC group levels of alanin aminotransferase (ALT), asparatate aminotransferase (AST), and malondialdehyde (MDA) increased (p < 0.001), whereas the levels of total glutathione (tGSH), glutathione peroxidase (GPO), and glutathione reductase (GRx) decreased (p < 0.001) compared to HG. In PPC group, the levels of ALT, AST, and MDA decreased (p < 0.001); and the levels of tGSH, GPO, and GRx increased (p < 0.003) compared to the PAC group. The histopathological examination showed dilated and congested blood vessels, hemorrhage, destruction, and edema in the liver tissue of PAC group. However, in the PPC group, mild dilatation and congestion were the only pathological findings. The results of our study indicate that pycnogenol® is a promising agent acting against paracetamol-induced liver toxicity.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2022THE EFFECT OF CARVACROL UPON EXPERIMENTALLY INDUCED DIABETIC NEUROPATHY AND NEUROPATHIC PAIN IN RATS
Ekinci, Bilge; Suleyman, Bahadir; Mammadov, Renad; Gezer, Arzu; Mendil, Ali Sefa; Akbas, Nergis; Bulut, Seval; Dal, Cagatay Nusret; Suleyman, Halis
Diabetic neuropathies are the most frequent complication of diabetes. While numerous metabolic pathways are disrupted in diabetic neuropathy, oxidative stress has been indicated as a significant reason for this condition. In this study, the effect of carvacrol, which has antioxidant effects, on experimental diabetic neuropathy and neuropathic pain was investigated. Alloxan was used to induce diabetes in the experiment. Diabetes was created by administering 120 mg/kg of alloxan intraperitoneally (i.p) once a day for 3 days. Rats with a blood glucose concentration above 250 mg/kg in the blood taken from the tail veins at the end of three days were considered diabetic. Rats were categorized under healthy control (HG), alloxan-induced hyperglycemia (AG), and alloxan-induced hyperglycemia + carvacrol-treated (ACG) groups. Carvacrol was i.p injected at 50 mg/kg dose to the ACG (n = 6) group of rats with hyperglycemia. The same volume of distilled water as the solvent was applied in the same way to AG (n = 6) and HG (n = 6) rat groups. This procedure was repeated once a day for three months. Carvacrol showed an anti-hyperglycemic effect in diabetic rats, a protective effect against lowering pain threshold, and analgesic activity in rat paws. Carvacrol prevented the oxidant/antioxidant balance from changing in favor of oxidants. The results supported that carvacrol is an agent against alloxan-induced peripheral diabetic neuropathic pain.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2022Amelioration of oxidative damage parameters by carvacrol on methanol-induced liver injury in rats
Gursul, Cebrail; Ozcicek, Adalet; Ozkaraca, Mustafa; Mendil, Ali Sefa; Coban, Taha Abdulkadir; Arslan, Aynur; Ozcicek, Fatih; Suleyman, Halis
The methanol metabolite that causes hepatotoxicity is formic acid, generating reactive oxygen radical formation and cell damage. Carvacrol is an antioxidant monoterpenic phenol produced from Thymus vulgaris. This study aimed to investigate the effects of carvacrol on methanol-induced oxidative liver damage in rats. Eighteen rats were divided into three groups. Methotrexate was administered orally for 7 days to methotrexate+methanol (MTM) and methotrexate+methanol+carvacrol (MMC) groups. Methotrexate was given before methanol to cause methanol poisoning. Distilled water was given to the healthy group (HG) as a solvent. At the end of the 7th day, 20% methanol was administered orally at a dose of 3 g/kg to the MTM and MMC groups. Four hours after methanol administration, 50 mg/kg carvacrol was injected intraperitoneally into the MMC group. Animals were sacrificed 8 h after carvacrol injection. Biochemical markers were studied in the excised liver tissue and blood serum samples, and histopathological evaluations were made. Severe hemorrhage, hydropic degeneration, pycnosis, and mononuclear cell infiltration were observed in the liver of the MTM group. Additionally, the levels of malondialdehyde (MDA), total oxidant status (TOS), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were significantly higher, and total glutathione (tGSH) and total antioxidant status (TAS) were significantly lower in the MTM group compared to HG (P<0.001). Carvacrol prevented the increase in MDA, TOS, ALT and AST levels with methanol and the decrease in tGSH and TAS levels (P<0.001), and alleviated the histopathological damage. Carvacrol may be useful in the treatment of methanol-induced liver damage.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2022The effects of taxifolin on neuropathy related with hyperglycemia and neuropathic pain in rats: A biochemical and histopathological evaluation
Alay, Murat; Sonmez, Miyase Gulcin; Sakin, Aysegul; Atmaca, Murat; Suleyman, Halis; Yazici, Gulce Naz; Coban, Abdulkadir; Suleyman, Bahadir; Bulut, Seval; Altuner, Durdu
Background. Hyperglycemia can be considered a determining factor in the development of diabetic neuropathy as well as neuropathic pain. There is a relationship between the excessive production of reactive oxygen species (ROS) and the pathogenesis of diabetic neuropathic pain. Taxifolin, on the other hand, is a flavonoid that has been documented to inhibit ROS production. Objectives. To investigate the effects of taxifolin, which has antioxidant and neuroprotective effects, on alloxan-induced hyperglycemia-induced neuropathy and neuropathic pain, biochemically and histopathologically. Materials and methods. The albino Wistar male rats were divided into 3 groups: Healthy group (HG), only alloxan group (AXG) and alloxan+taxifolin group (ATG). Hyperglycemia in animals was caused through intraperitoneal injection of alloxan at a dose of 120 mg/kg. Paw pain thresholds of animals were measured using Basile algesimeter. Sciatic nerve tissues were examined biochemically and histopathologically in order to evaluate neuropathy. Results. Our experimental results revealed that taxifolin significantly prevented the increase of plasma glucose concentration level with alloxan administration, the decrease of the paw pain threshold related to hyperglycemia, the change of oxidant-antioxidant balance in the sciatic nerve tissue in favor of oxidants, and the deterioration of tissue morphology in animals. Conclusions. Our experimental results indicate that taxifolin alleviates alloxan-induced hyperglycemia-related neuropathy and neuropathic pain.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2022Effect of thiamine pyrophosphate on cyclophosphamide-induced oxidative ovarian damage and reproductive dysfunction in female rats
Ozer, Mehmet; Ince, Sefa; Gundogdu, Betul; Aktas, Mehmet; Uzel, Kemine; Gursul, Cebrail; Suleyman, Halis; Suleyman, Zeynep
Background. Cyclophosphamide is a drug used in various types of cancer. It can cause oxidative and inflammatory ovarian damage and infertility. Thiamine pyrophosphate (TPP) to be investigated for its effect on cyclophosphamide-induced ovarian damage and reproductive dysfunction in the present study is the active metabolite of thiamine. It has been shown that TPP protects organs and tissues from oxidative stress and proinflammatory cytokine damage. Objectives. To investigate the effect of TPP against the ovarian damage and reproductive dysfunction caused by cyclophosphamide in rats. Materials and methods. Albino Wistar type female rats were divided into healthy control (HG), cyclophosphamide (CYC) and TPP + cyclophosphamide (TPPC) groups (for each group, n = 12). Thiamine pyrophosphate at a dose of 25 mg/kg was injected intraperitoneally (ip.) in the TPPC group, and 0.9% NaCI solution was injected ip. in the CYC and HG groups. One hour after the injection, 75 mg/kg of cyclophosphamide was administered ip. in the TPPC and CYC groups. This procedure was repeated once a day for 30 days. At the end of this period, 6 rats from each group were euthanized with a high dose of anesthetic (50 mg/kg of sodium thiopental). Biochemical and histopathological examinations were performed on the extracted ovarian tissue. The remaining animals were kept in the laboratory with mature male rats for 2 months for reproduction. Results. Thiamine pyrophosphate significantly decreased the cyclophosphamide-induced increase in the levels of the oxidant parameter malondialdehyde (MDA), proinflammatory nuclear factor kappa B (NF-κB), tumor necrosis factor alpha (TNF-α), and interleukin 1 beta (IL-1β). In addition, TPP decreased the severe histopathological damage associated with cyclophosphamide in the ovarian tissue and prevented infertility. Conclusions. Our experimental results have suggested that TPP could be beneficial in the treatment of cyclophosphamide-induced ovarian injury and infertility.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2022Effect of Usnea longisima Ethyl Acetate Extract on Staphylococcus aureus-Induced Experimental Rhinosinusitis in Rats
Dilber, Muhammet; Erhan, Ertugrul; Bulut, Seval; Kuzucu, Mehmet; Akyuz, Sumeyye; Suleyman, Halis
SUMMARY. The role of oxidant and proinflammatory cytokines in the pathogenesis of rhinosinusitis induced by Staphylococcus aureus has been demonstrated in studies. Usnea longissima ethyl acetate extract (ULE) was obtained from this lichen species. ULE is known to have antibacterial, antioxidant and anti-inflammatory effects. The objective of the study is to investigate the effect of ULE on S. aureus-induced experimental rhinosinusity in rats and compare it with cefazolin. Anesthesia was created through virtue of intraperitoneal injection of 25 mg/kg thiopental sodium to male albino Wistar rats. Then the rats were divided into groups as healthy (HG), ones inoculated with S. aureus (RSG) to their nostril, ones inoculated with S. aureus to their nostril + ULE (REA) applied and ones with S. aureus inoculated to their nostril + cefazolin (RCG) applied. Our biochemical test results revealed the fact that ULE was able to inhibit inflammatory mediators such as nuclear factor kappa B (NF-κB), tumor necrosis factor alpha (TNF-α), an interleukin 1 beta (IL-1β) and cyclooxygenase-2 (COX-2) increase with S. aureus in sinonasal tissue better than cefazolin. Moreover, while ULE significantly suppressed the increase in the amount of malondialdehyde (MDA), the oxidant parameter in the sinonasal tissue, and the decrease in the antioxidant total glutathione (tGSH) and cyclooxygenase-1 (COX-1), cefazolin could not. ULE and cefazolin were observed to be effective on standard bacteria. From our experimental results, it is understood that ULE prevents oxidative and inflammatory damage induced by S. aureus in the sinonasal tissue better than cefazolin.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2022Ameliorative effects of Liv-52 on doxorubicin-induced oxidative damage in rat liver
Yildirim, Nilgun; Lale, Azmi; Yazıcı, Gulce Naz; Sunar, Mukadder; Aktas, Mehmet; Ozcicek, Adelet; Suleyman, Bahadır; Ozcicek, Fatih; Suleyman, Halis
Hepatotoxicity is a common side effect of doxorubicin (Dox) treatment of cancer. Liv-52 is an ayurvedic medicine that is reported to ameliorate liver injury due to oxidative stress. We investigated the effects of Liv-52 on Dox induced oxidative damage to liver tissues of rats using biochemical and histopathological techniques. Thirty male rats were assigned randomly into three equal groups: control (CG), Dox group (DG) Liv-52 + Dox group (LD). Rats in the LD group received 50 mg/kg Liv-52 in distilled water via gastric gavage. Distilled water was given via the same route to the rats in the DG and CG groups. Rats in the LD and DG groups were injected intraperitoneally with 5 mg/kg Dox 1 h after administration of Liv-52 or distilled water. The procedure was repeated daily for 7 days. On day 8, the animals were sacrificed, and serum and tissue biochemical and histopathological assays were performed. The malondialdehyde level was increased significantly in the DG group, while glutathione and superoxide dismutase levels were significantly lower in the DG group compared to the LD and CG groups. The highest levels of alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase were found in the DG group, while the lowest levels were found in the CG group, which exhibited levels similar to those of the LD group. Treatment with Liv-52 prior to Dox treatment reduced the histopathologic changes in the Dox group. Therefore, pre-treatment with Liv-52 protected against Dox induced oxidative stress and hepatotoxicity.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2022Effect of taxifolin on acrylamide-induced oxidative and proinflammatory lung injury in rats: Biochemical and histopathological studies
Demir, Omer Faruk; Elma, Bekir; Suleyman, Bahadir; Ozkaraca, Mustafa; Mammadov, Renad; Suleyman, Halis; Coban, Taha Abdulkadir; Ozcicek, Fatih
Purpose: To examine the probable beneficial effects of taxifolin against acrylamide damage in lung tissue. Methods: 18 male albino Wistar rats were divided into healthy (HG), acrylamide (AG) and taxifolin + acrylamide (TAG) groups. Once a day for 30 days, acrylamide was orally administered to the AG group (50 mg/kg), while ACL (50 mg/kg) and TAX (20 mg/kg) were orally administered to TAG group. Protein concentration, malondialdehyde (MDA), and total glutathione (tGSH) levels as well as oxidant and antioxidant molecules concentrations of the rat lung tissues were measured. In addition, degree of mononuclear (MN) cell infiltration and bronchial-associated lymphoid tissue (BALT) hyperplasia was evaluated by the degree of hyperplasia (absent, mild, moderate, severe). The histopathological and biochemical data the groups were compared. Results: When compared in terms of MDA levels, it was found that the AG group had high MDA levels, and the TAG group had low MDA levels. (p < 0.001). TAG group was found to have a higher tGSH level than the AG group (p < 0.001). Compared to the AG group, lower TOS and higher TAS levels were obtained in the TAG group (p < 0.001). In addition, when TOS levels of TAG and HG groups were compared, the TOS levels between the two groups were statistically insignificant (p = 0.213). It has been observed that TAX administration prevents the increase in NF-ƘB level. When the NF-ƘB levels of the AG and TAG groups were compared with each other, there was a statistically significant difference (p = 0.001). In the AG group, severe MN cell hyperplasia and BALT hyperplasia were observed histopathologically. It was determined that these findings were alleviated in the TAG group. A histopathologically significant difference was found between AG and TAG groups (p < 0.05). Conclusion: Taxifolin has beneficial effects against lung injury caused by acrylamide, a health-damaging environmental factor. Regular use of taxifolin can be recommended, especially in people who are known to have intense contact with acrylamide. There is a need for research studies on this subject.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2021Everolimus-Induced Oral Mucositis Can be Prevented by Hippophae Rhamnoides Extract in Rats
Gezer, Arzu; Cil, Ibrahim; Suleyman, Bahadir; Ozcicek, Fatih; Semiz, Huseyin Salih; Akagunduz, Baran; Ozer, Muhammet; Demircan, Nazım Can; Aktas, Mehmet; Suleyman, Halis
Objectives: Oral mucositis is a significant toxicity related to the mammalian target of rapamycin inhibitor everolimus. Oxidative stress and pro-inflammatory cytokines, which contribute to treatment-related mucositis, can betargeted with Hippophae rhamnoides extract (HRE). Herein, we assessed the effects of HRE on everolimus-inducedmucositis in rats.Methods: Eighteen rats were equally divided into healthy, everolimus, and everolimus plus HRE groups. Malondialdehyde (MDA) and total glutathione (tGSH) levels along with interleukin-1 beta (IL-1β) and tumor necrosis factor-alpha(TNF-α) gene expression levels were measured in the tongue and buccal mucosa tissues of all groups, histopathologicalchanges were also evaluated. We tested the significance of variations with one-way variance analysis. We also analyzedthe differences between groups with Kruskal–Wallis test and Mann–Whitney U-test.Results: HRE significantly decreased MDA and increased tGSH levels and reduced IL-1β and TNF-α gene expression inboth tissues administered everolimus (p<0.001 for each). Histological examination revealed that HRE improved epithelial formation and keratinization, disrupted by everolimus, and alleviated everolimus-related mononuclear cell infiltration (p<0.05 for each).Conclusion: In light of these results, HRE may be a promising agent to manage oral mucositis caused by everolimus,given the lack of effective therapeutic options for this type of adverse event.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2021The effect of lutein on vancomycin-induced oxidative kidney damage in rats
Dabanlıoğlu, Bülent; Sener, Ebru; Ozcicek, Adalet; Aktaş, Mehmet; Akyüz, Sümeyye; Suleyman, Halis; Kocaturk, Hüseyin; Bedir, Fevzi; Karahan, Sevil
Aim: It has been reported that nephrotoxicity is observed in 5-25% of patients during vancomycin use. The part oxidative stress plays in the pathogenesis of vancomycin-induced nephrotoxicity has been demonstrated. Lutein shows antioxidant activity. The purpose of the present study was to evaluate effects experienced by lutein on oxidative kidney damage induced by vancomycin in rats biochemically an histopathologically. Materials and Methods: Oral administration of lutein at a rate of 1mg per kg was performed for the lutein plus vancomycin group (L+VAN) (n=6), whereas saline was administered to the vancomycin (VAN) (n=6) and healthy control (C) (n=6) groups. Sixty minutes after administration of lutein and solvent, 200 mg/kg vancomycin was intraperitoneally injected twice a day to L+VAN and VAN groups. This procedure was repeated for 7 days. Then malondialdehyde (MDA), total glutathione (tGSH), total oxidant system (TOS) and total antioxidant system (TAS) levels have been assessed in kidney tissues; in addition, creatinine and BUN were measured in blood samples. Kidney tissues were also evaluated histopathologically. Results: Significant histopathological damage could be seen in the kidney tissue of the VAN group that increasing MDA and TOS and creatinine and BUN. Histopathological damage was not very significant in the L+VAN group that decreasing MDA, TOS, BUN and creatinine levels. Conclusion: This suggests that lutein may be effective for the treatment of the treatment of vancomycin-associated oxidative kidney damage.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2021Taxifolinin Sıçanlarda Amikasin ile İndüklenen Ototoksisiteye Karşı Koruyucu Etkisi: Biyokimyasal ve Histopatolojik Değerlendirme
Gürsul, Cebrail; Çoban, T. Abdulkadir; Salcan, Ismail; Suleyman, Halis; Çımen, Ferda Keskın; Erhan, Ertugrul; Dilber, Muhammet; Karahan, Sevil
Amaç: Amikasin, yarı sentetik aminoglikozid türevi antibakteriyel bir ilaçtır. Amikasinin kalıcı işitme kaybına yol açan kokleotoksik etkileri rapor edilmiştir. Amikasin ototoksisitesinden, reaktif oksijen türlerinin (ROS) aşırı üretimi sorumlu tutulmaktadır. Çalışmamızda amikasin ile indüklenen ototoksisiteye karşı koruyucu etkisini araştıracağımız Taxifolin (3.5.7,3,4-pentahydroxy flavanone) antioksidan bir flavonoiddir. Taxifolinin albino Wistar türü erkek sıçanlarda amikasin ile indüklenen ototoksisiteye karşı koruyucu etkisini biyokimyasal ve histopatolojik olarak araştırmak amaçlanmaktadır. Materyal ve Metot: Hayvanların TAK grubuna taxifolin 50 mg/kg (n-6) oral yoldan gavajla verildi. AMK (n-6) ve HG (n-6) gruplarına ise çözücü olarak distile su aynı hacimde aynı yöntemle uygulandı. Taxifolin ve distile su verildikten bir saat sonra TAK ve AMK gruplarına amikasin 200 mg/kg intramusküler yoldan enjekte edildi. Bu prosedür günde bir defa 14 gün boyunca uygulandı. Bu süre sonunda yüksek doz ketamile (120 mg/kg) öldürülen hayvanlardan çıkarılan koklea dokuları biyokimyasal ve histopatolojik olarak incelendi. Bulgular: Malondialdehid (MDA) ve total oksidan statusun (TOS) yüksek, total glutatyon (tGSH) ve total antioksidan statüsun (TAS) düşük bulunduğu AMK grubunun koklea dokusunda ödem ve destrüksiyon gibi histopatolojik hasar görülmüştür. Ancak, MDA, TOS, tGSH ve TAS düzeyleri HG grubuna yakın olan TAK grubunda hafif ödem dışında herhangi bir histopatolojik bulgulara rastlanmadı. Sonuç: Taksifolin, amikasinle ilişkili koklea hasarının önlenmesinde yararlı olabileceğini işaret etmektedir.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2021Protective effect of nimesulide on the external ear damage induced by staphylococcusaureus inoculation in rats
Mammadov, Renad; Suleyman, Bahadir; Çoban, T. Abdulkadir; Salcan, Ismail; Akyüz, Sümeyye; Suleyman, Halis; Erhan, Ertugrul; Dilber, Muhammet
Since the external ear is covered with skin, infections that cause otitis externa are often produced by distinct skin flora in several different areas. Staphylococcus aureus (S. aureus) reproduces the most out of all bacteria isolated from external auditory canal skin culture samples. Proinflammatory cytokines are the main components responsible for tissue damage pathogenesis due to S. aureus. Nimesulide is a nonsteroidal anti-inflammatory drug that selectively inhibits cyclooxygenase-2 and also demonstrates antioxidant properties. The present study aimed to examine the antibacterial activity of nimesulide against S. aureus and to compare its effectiveness on otitis externa induced by S. aureus in male albino Wistar rats with that of cefazolin. The antimicrobial activity testing was conducted using the Kirby-Bauer disk diffusion method as described by the European Committee on Antimicrobial Susceptibility Testing. To induce otitis externa, we applied 0.5 ml of S. aureus to the ear skin using a hypodermic syringe (S. aureus strain TACK 25923 was dispersed on the 900x10-6 concentration of colony forming units per ml). Nimesulide and cefazolin were administered orally at a dose of 50 mg/kg. The antibacterial activity of cefazolin when used in equal doses (50 µg/ml) was more powerful against S. aureus than nimesulide. However, nimesulide reduced the macroscopic findings (such as oedema and redness) induced by S. aureus better than cefazolin. Additionally, nimesulide inhibited the increase of oxidant and proinflammatory indicators related to S. aureus, while cefazolin was able to inhibit only the increase in proinflammatory indicators. Our results showed that nimesulide was superior to cefazolin in terms of reducing the external ear damage caused by S. aureus. Therefore, nimesulide monotherapy or a combination of nimesulide and cefazolin therapy may be beneficial in the treatment of bacterial otitis externa.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2021Usnea Longissima Ekstresinin Akciğer Kanseri Hücre Kültürüne (A549) Etkisi
Eksi, Saliha; Altuner, Durdu; Suleyman, Halis; Turumtay, Emine Akyuz
Amaç: Bazı likenlerin antikanser aktivitesi olduğu bilinmektedir. Ancak, Usnea longisima liken türünün insan akciğer kanser \rhücrelerine (A549) karşı aktivitesine ait bilgilere rastlanmadı. Bu \rçalışmanın amacı Usnea longisima’nın, A549 hücre hattına etkisini araştırmaktır.\rGereç ve Yöntem: Usnea longisima ekstresinin kültür ortamında A549 hücre morfolojisine etkisi 400 µg/ml konsantrasyonda araştırıldı ve Olympus inverted mikroskobu (CK x 41, 10 x) ile \rincelendi. Usnea longisima ekstresinin sitotoksik aktivitesi 400, \r200, 100, 50, 25, 12.5 ve 6,75 µg/ml kosantrasyonlarda 1983’te \rMosmann tarafından yayınlanan protokole göre test edildi.\rBulgular: DMSO A549 hücre hattı üzerinde toksik etki oluşturmamıştır. Ancak, Usnea longisima ekstresi uygulanan A549 \rhücrelerin tamamına yakını ölmüştür. Ayrıca, Usnea longisima \rekstresinin hücreleri yüzeyden kopararak ölümüne neden olduğu anlaşılmaktadır.\rSonuç: Usnea longisima ekstresi, in vitro ortamda A549 kanser \rhücreleri üzerinde doza bağlı olarak sitotoksik etki oluşturmuştur. Bu nedenle akciğer kanserlerinin tedavisinde faydalı olabileceği düşünülmektedir.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2021Biochemical and Histopathological Evaluation of Sunitinib Effect on Ovarian Injuries by Ischemia-Reperfusion in Rats
Ince, Sefa; Ozer, Mehmet; Kadioglu, Berrin Goktug; Kuzucu, Mehmet; Gundogdu, Betul; Gursul, Cebrail; Suleyman, Zeynep; Suleyman, Halis
Background and Objective: Interleukin 1 beta (IL-1 beta) and tumour necrosis factor-alpha (TNF-alpha) as the proinflammatory cytokines, whose production increases during reperfusion after ischemia (I/R), were reported to produce the Reactive Oxygen Species (ROS). It was reported that sunitinib has proinflammatory cytokine expression suppression and antioxidant effects. The study aims to investigate the effects of sunitinib on ovarian injuries created by I/R in albino Wistar female rats biochemically and histopathologically. Materials and Methods: Animals divided into 3 groups as sunitinib+ovarian tissues (SOIR), I/R ovarian tissues (OIR) and Sham operation (SG) groups. There were ischemia and reperfusion procedures for 2 hrs on the right ovaries of the OIR and SOIR group rats. Results: The Nuclear Factor kappa B (NF-kappa B), Tumour Necrosis Factor-alpha (TNF-alpha), Malondialdehyde (MDA) and Interleukin 1 beta (IL-1 beta) levels in the OIR group's ovarian tissue were high whereas their total glutathione (tGSH) level was found to be low compared to the SOIR and SG group. While distinct histopathologic injuries were seen in the ovarian tissue of the OIR group, histopathologic injuries in the SOIR group were mild. Conclusion: Sunitinib prevented the oxidant and proinflammatory cytokine increase and antioxidant decrease related to I/R. It is concluded that sunitinib might be used for the treatment of ovarian I/R injuries.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2021The effect of adenosine triphosphate on propofol-induced myopathy in rats: A biochemical and histopathological evaluation
Erdem, Kezban Tuna Ozkaloglu; Bedir, Zehra; Ates, Irem; Kuyrukluyildiz, Ufuk; Coban, Taha Abdulkadir; Yazici, Gulce Naz; Arslan, Yusuf Kemal; Suleyman, Zeynep; Suleyman, Halis
Propofol infusion syndrome characterized by rhabdomyolysis, metabolic acidosis, kidney, and heart failure has been reported in long-term propofol use for sedation. It has been reported that intracellular adenosine triphosphate (ATP) is reduced in rhabdomyolysis. The study aims to investigate the protective effect of ATP against possible skeletal muscle damage of propofol in albino Wistar male rats biochemically and histopathologically. PA-50 (n = 6) and PA-100 (n = 6) groups of animals was injected intraperitoneally to 4 mg/kg ATP. An equal volume (0.5 ml) of distilled water was administered intraperitoneally to the P-50, P-100, and HG groups. One hour after the administration of ATP and distilled water, 50 mg/kg propofol was injected intraperitoneally to the P-50 and PA-50 groups. This procedure was repeated once a day for 30 days. The dose of 100 mg/kg propofol was injected intraperitoneally to the P-100 and PA-100 groups. This procedure was performed three times with an interval of 1 days. Our experimental results showed that propofol increased serum CK, CK-MB, creatinine, BUN, TP I, ALT, AST levels, and muscle tissue MDA levels at 100 mg/kg compared to 50 mg/kg and decreased tGSH levels. At a dose of 100 mg/ kg, propofol caused more severe histopathological damage compared to 50 mg/ kg. It was found that ATP prevented propofol-induced muscle damage and organ dysfunction at a dose of 50 mg/kg at a higher level compared to 100 mg/kg. ATP may be useful in the treatment of propofol-induced rhabdomyolysis and multiple organ damage.
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