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- ArticleYalnız künye2022The Effect of Thiamine Pyrophosphate and Tocilizumab Alone and in Combination upon Ethanol-Induced Peripheral Neuropathy and Neuropathic Pain in Rats
Ekinci, Bilge; Suleyman, Bahadir; Mammadov, Renad; Cankaya, Murat; Yazici, Gulce Naz; Suleyman, Zeynep; Dal, Cagatay Nusret; Ergul, Emine Esra; Onk, Didem; Suleyman, Halis
The aim of the study to determine the effects of thiamine pyrophosphate (TPP) and tocilizumab (TCZ) either alone or in combination on ethanol-induced neuropathic pain and peripheral neuropathy in rat nerve tissues, biochemically and histopathologically. Albino Wistar male rats were divided into five groups as the healthy (HG), ethanol alone (EtOH), TPP + ethanol (TPE), TCZ + ethanol (TCE), and TPP + TCZ + ethanol (TPTE) groups. TPE (n = 6), TCE (n = 6) and TPTE (n = 6) groups were injected with 20 mg/kg TPP, 8 mg/kg TCZ, and 20 mg/kg TPP+8 mg/kg TCZ intraperitoneally (respectively). Distilled water were given the HG (n = 6) and EtOH (n = 6) groups. One hour after the drugs and distilled water were administered, 35% ethanol was administered orally by lavage at a 5 g/kg dose to the TPE, TCE, TPTE, and EtOH groups. Paw pain thresholds of animals were measured using Basile Algesimeter. TPTE, which suppressed the decrease of the pain threshold with ethanol more significantly than TPP and TCZ. The best inhibitor of MDA increase and inhibit the reduction of tGSH by etanol was listed as TPTE > TPE > TCZ. The potency of suppressing the increase in cytokine production with ethanol was listed as TPTE > TCE > TPE. In conclusion, the results indicate that TPP+TCZ may be more beneficial than TPP and TCZ alone in treating ethanol-related neuro-pathic pain.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2022The Molecular Mechanism of the Effect of Carvacrol on Desflurane Inhalation-Induced Liver Damage in Rats
Disli, Zeliha Korkmaz; Acun Delen, Leman; Tas, Hakan Gokalp; Kuyrukluyildiz, Ufuk; Disli, Olcay Murat; Yazici, Gulce Naz; Coban, Abdulkadir; Suleyman, Halis
The metabolite of desflurane has been linked to hepatotoxicity. Carvacrol possesses antioxidant, antibacterial, antifungal, anticancer, anti-inflammatory, and spasmolytic properties, according to research. Our goal is to demonstrate that carvacrol protects rats’ livers against repeated doses of desflurane. Healthy (HG), desflurane (DS), and carvacrol + desflurane treatment (CDS) groups were formed from 18 albino male Wistar rats. A single dosage of carvacrol and 6% desflurane was given for 2 h on the 0th and 8th days. In the CDS group, the ALT and AST, MDA, TOS, NF-xB, TNF-α IL1 β levels were lower compared to the DS group (p < 0.001). The tGSH,TAS levels were found to be higher in the CDS group compared to the DS group (p < 0.001). It was determined that the mean degeneration level, Kupffer cell activation and PMNL infiltration were found to be lower compared to the DS group (p < 0.05).We confirmed that carvacol can be used in the treatment of desflurane-related liver injury.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2022The effect of taxifolin on experimental testicular ischaemia reperfusion injury in rats. A biochemical and histopathological analysis; [Efecto de la taxifolina en la lesión experimental testicular por reperfusión-isquemia en ratas. Análisis bioquímico e histopatológico]
Bedir, Fevzi; Kocaturk, Huseyin; Ozgeris, Fatma Betul; Yazici, Gulce Naz; Suleyman, Zeynep; Suleyman, Halis
Objectives: The aim of the study is to investigate the protective effect of taxifolin (3,5,7,3,4-pentahydroxy flavanone), a strong antioxidant, against testicular I/R injury in rats biochemically and histopathologically. Materials and methods: 50 mg/kg taxifolin was administered to taxifolin + testicular torsion–detorsion (TTTD, n-10) group of Albino Wistar male rats by oral gavage. Distilled water .5 ml as a solvent was administered to testicular torsion–detorsion (TTD, n-10) and Healthy Control (SG, n-10) groups using the same method. An hour after the administration of taxifolin and distilled water, anaesthesia (ketamine 60 mg/kg) was administered to all animal groups. TTD and TTTD group animals were subjected to testicular torsion at 720 degrees for four hours during anaesthesia. At the end of this period, testicular detorsion was applied and perfusion was allowed for four hours. Sham operation was applied to SG group. Results: Our biochemical experiment results showed that the amount of malondialdehyde (MDA) in testicular tissue of TTD group presented a significant increase compared to SG and TTTD groups whereas total glutathione (tGSH) and superoxide dismutase (SOD) levels decreased. In addition, while TTD group presented severe histopathological damage in germinal epithelium cell and seminiferous tubule, mild damage was observed in TTTD group. Conclusions: The results of our experiment indicate that taxifolin could be useful in the treatment of testicular I/R damage.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2022Effect of sunitinib on testicular oxidative and proinflammatory damage induced by ischemia–reperfusion in rats; [Efecto de sunitinib en el daño oxidativo y proinflamatorio testicular inducido por isquemia-reperfusión en ratas]
Keskin, Ercument; Erdogan, Abdullah; Suleyman, Halis; Yazici, Gulce Naz; Sunar, Mukadder; Gul, Mehmet Ali
Introduction: This study aimed to biochemically and histopathologically investigate the effect of sunitinib on oxidative testicular damage induced by ischemia/reperfusion in rats. Material-method: Experimental animals were divided into three groups of six rats each: testicular torsion–detorsion (TTD), sunitinib + testicular torsion–detorsion (STD), and sham control (SC). Sunitinib (25 mg/kg) was administered orally to the STD group by gavage. Normal saline (0.9% NaCl) was administered orally to the TTD and control groups as the solvent. One hour after administration of sunitinib and 0.9% NaCl, all animal groups were done torsion–detorsion. Then, all the rats were killed by high-dose anesthesia, and their testicles were removed. Biochemical and histopathological examinations were performed on the removed testicular tissues. Results: Malondialdehyde; it was observed that the results in the STD group were close to those of the SC group and statistically significant lower compared to the TTD group (p = 0.001). The glutathione values were statistically significantly higher in the STD group compared to the TTD group (p < 0.001). Nuclear factor kappa B values, revealing a statistically significant difference between the TTD and STD groups (p < 0.001). The TNF-α levels were measured and indicating that the results of the STD group were statistically significantly lower than those of the TTD group (p < 0.001). Histopathologically, animal tissues given sunitinib were observed to resemble normal tissues. Conclusion: Sunitinib was shown to prevent histopathological changes in testicular tissue against ischemia/reperfusion damage.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2022The effects of taxifolin on neuropathy related with hyperglycemia and neuropathic pain in rats: A biochemical and histopathological evaluation
Alay, Murat; Sonmez, Miyase Gulcin; Sakin, Aysegul; Atmaca, Murat; Suleyman, Halis; Yazici, Gulce Naz; Coban, Abdulkadir; Suleyman, Bahadir; Bulut, Seval; Altuner, Durdu
Background. Hyperglycemia can be considered a determining factor in the development of diabetic neuropathy as well as neuropathic pain. There is a relationship between the excessive production of reactive oxygen species (ROS) and the pathogenesis of diabetic neuropathic pain. Taxifolin, on the other hand, is a flavonoid that has been documented to inhibit ROS production. Objectives. To investigate the effects of taxifolin, which has antioxidant and neuroprotective effects, on alloxan-induced hyperglycemia-induced neuropathy and neuropathic pain, biochemically and histopathologically. Materials and methods. The albino Wistar male rats were divided into 3 groups: Healthy group (HG), only alloxan group (AXG) and alloxan+taxifolin group (ATG). Hyperglycemia in animals was caused through intraperitoneal injection of alloxan at a dose of 120 mg/kg. Paw pain thresholds of animals were measured using Basile algesimeter. Sciatic nerve tissues were examined biochemically and histopathologically in order to evaluate neuropathy. Results. Our experimental results revealed that taxifolin significantly prevented the increase of plasma glucose concentration level with alloxan administration, the decrease of the paw pain threshold related to hyperglycemia, the change of oxidant-antioxidant balance in the sciatic nerve tissue in favor of oxidants, and the deterioration of tissue morphology in animals. Conclusions. Our experimental results indicate that taxifolin alleviates alloxan-induced hyperglycemia-related neuropathy and neuropathic pain.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2021Ischemia-Reperfusion Injury of Sciatic Nerve in Rats and Protective Role of Benidipine Hydrochloride
Çakir, Tayfun; Yücetas, Seyho Cem; Yazici, Gulce Naz; Sunar, Mukadder; Arslan, Yusuf Kemal; Kayaci, Selim; Süleyman, Halis
Objective: Benidipine is an antihypertansive agent which elicits vasodilatation of arteries by blocking Ca2+. The aim of this study is to investigate the neuroprotective effects of benidipine on rat sciatic nerves exposed to ischemia and reperfusion (I/R). Method: 30 male Wistar Albino rats were used in this study. The rats were divided into three groups(n=10 per group). I/R procedure was administered to the IR group, 10 mu g/kg/day, i.v. benidipine was given for 2 hours (h) to the BIR group before I/R procedure. And a sham group (SG) was created. Next, histopathological and biochemical investigations were performed on sciatic nerve tissues. Superoxide dismutase (SOD), Malondialdehyde (MDA) and glutathione (GSH) were analyzed as oxidative stress markers; interleukin 1 beta (IL-1 beta) and tumor necrosis factor alpha (TNF-alpha) were analyzed as inflammatory stress markers in biochemical tests. Results: Axonal swelling, myelin loss and disorganisation were seen in the IR group. Schwann cells showed hypertrophy, hyperplasia, blood vessels were seen as congested. But these results were under normal values and almost similiar between BIR and SG groups. In the BIR group ischemic fibere degeneration score was significiantly lower than IR group. And MDA, TNF-alpha, IL-1 beta values higher, GSH and SOD values were lower in the IR group, but in the BIR group all these values were kept within normal limits. Conclusion: This study showed that benidipine reduced oxidative stress and inflammation in rat sciatic nerve after I/R injury.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2021Effect of Rutin on Gentamicin-Induced Ototoxicity in Rats: A Biochemical and Histopathological Examination
Salcan, Ismail; Dilber, Muhammet; Bayram, Rana; Süleyman, Esma; Yazici, Gulce Naz; Çoban, Abdulkadir; Süleyman, Halis
Objective: Gentamicin is a broad-spectrum aminoglycoside antibiotic administered parenterally for moderate to severe gram-negative infections. Ototoxicity is an important side effect that limits gentamicin use. The aim of this study is to investigate the effect of rutin on gentamicin-induced ototoxicity in rats biochemically and histopathologically. Methods: Distilled water was administered by oral gavage to healthy controls (HG) and cobalt administered group (GC). 50 mg/kg rutin was administered by oral gavage to rutin + gentamicin (RGG) group. After one hour, 100 mg/kg gentamicin was injected intraperitoneally (i.p) to the RGG and GC animal groups. This procedure was repeated once a day for 14 days. Results: Malondialdehyde (MDA), nuclear factor-kappa B(NF-kappa B), tumor necrosis factor alpha (TNF-alpha) and interleukin-1 beta(IL-1 beta) levels in the cochlear nerve tissue of gentamicin-treated animals were significantly higher compared to healthy controls and rutin + gentamicin treated rats. On the other hand, the amount of total Glutathione (tGSH) was significantly lower compared to the control and rutin group. Histopathological examination revealed degenerated myelinated nerve fibers in the gentamicin group and Schwann cell nuclei were generally not seen. There was a high accumulation of collagen fiber in the tissue and dilated blood capillaries. In the rutin group, myelinated nerve fibers mostly exhibited normal morphology, Schwann cell nuclei were evident and the vessels were normal. Conclusion: Our experimental results suggest that rutin may be effective in the treatment of gentamicin-associated ototoxicity.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2021The effect of adenosine triphosphate on propofol-induced myopathy in rats: A biochemical and histopathological evaluation
Erdem, Kezban Tuna Ozkaloglu; Bedir, Zehra; Ates, Irem; Kuyrukluyildiz, Ufuk; Coban, Taha Abdulkadir; Yazici, Gulce Naz; Arslan, Yusuf Kemal; Suleyman, Zeynep; Suleyman, Halis
Propofol infusion syndrome characterized by rhabdomyolysis, metabolic acidosis, kidney, and heart failure has been reported in long-term propofol use for sedation. It has been reported that intracellular adenosine triphosphate (ATP) is reduced in rhabdomyolysis. The study aims to investigate the protective effect of ATP against possible skeletal muscle damage of propofol in albino Wistar male rats biochemically and histopathologically. PA-50 (n = 6) and PA-100 (n = 6) groups of animals was injected intraperitoneally to 4 mg/kg ATP. An equal volume (0.5 ml) of distilled water was administered intraperitoneally to the P-50, P-100, and HG groups. One hour after the administration of ATP and distilled water, 50 mg/kg propofol was injected intraperitoneally to the P-50 and PA-50 groups. This procedure was repeated once a day for 30 days. The dose of 100 mg/kg propofol was injected intraperitoneally to the P-100 and PA-100 groups. This procedure was performed three times with an interval of 1 days. Our experimental results showed that propofol increased serum CK, CK-MB, creatinine, BUN, TP I, ALT, AST levels, and muscle tissue MDA levels at 100 mg/kg compared to 50 mg/kg and decreased tGSH levels. At a dose of 100 mg/ kg, propofol caused more severe histopathological damage compared to 50 mg/ kg. It was found that ATP prevented propofol-induced muscle damage and organ dysfunction at a dose of 50 mg/kg at a higher level compared to 100 mg/kg. ATP may be useful in the treatment of propofol-induced rhabdomyolysis and multiple organ damage.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2021The effect of dexmedetomidine on gastric ischemia reperfusion injury in rats. Biochemical and histopathological evaluation
Kuyrukluyildiz, Ufuk; Delen, Leman Acun; Onk, Didem; Yazici, Gulce Naz; Gulaboglu, Mine; Suleyman, Halis
Purpose: To evaluate the protective effect of dexmedetomidine on gastric injury induced by ischemia reperfusion (I/R) in rats. Methods: A total of 18 male albino Wistar rats were divided groups as: gastric ischemia reperfusion (GIR), gastric ischemia reperfusion and 50 μg/kg dexmedetomidine (DGIR) and sham operation (HG) group. After the third hour of reperfusion, the biochemical and histopathological examinations were performed on the removed stomach tissue. Results: Malondialdehyde (MDA) and myeloperoxidase (MPO) levels were found to be significantly higher in GIR compared to HG (p < 0.05). A statistically significant decrease was observed at the DGIR compared to the GIR for oxidants levels. Total glutathione (tGSH) and superoxide dismutase (SOD) levels were statistically significantly decreased at the GIR, and antioxidants levels were found to be significantly higher in the DGIR (p < 0.05) There was no significant difference between HG and DGIR in terms of SOD (p = 0.097). The DGIRs’ epitheliums, glands and vascular structures were close to normal histological formation. Conclusions: Dexmedetomidine is found to prevent oxidative damage on the stomach by increasing the antioxidant effect. These results indicate that dexmedetomidine may be useful in the treatment of ischemia-reperfusion-related gastric damage.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2021Effects of Benidipine Hydrochloride on Ischemia Reperfusion Injury of Rat Brain
Cakir, Tayfun; Yucetas, Seyho Cem; Yazici, Gulce Naz; Sunar, Mukadder; Arslan, Yusuf Kemal; Suleyman, Halis
AIM: To evaluate the neuroprotective effects of benidipine hydrochloride on the cerebral cortex tissues in rats exposed to cerebral ischemia-reperfusion (I/R) injury. MATERIAL and METHODS: Twenty-four male Wistar albino rats were randomly divided into three groups, and administered benidipine hydrochloride (10 µg/kg/day) orally through a catheter for 2 h to form the study group (BIR group, n=8). The I/R procedure was performed in the rats of the IR group (n=8), and a sham group was formed to determine the normal structure of the cerebral cortex (n=8). Transient ischemia was performed by clamping the left common carotid artery for 2 h. Subsequently, reperfusion was applied for 12 h. Cerebral infarct volumes were measured and cerebral cortex tissue samples were analyzed histopathologically and biochemically by measuring malondialdehyde (MDA), total glutathione, cyclooxygenase 1 (COX-1), COX-2 and superoxide dismutase (SOD) RESULTS: The infarct area was markedly reduced in the BIR group vs. the IR group. Histopathologically, neuroprotective effects of benidipine hydrochloride were observed in the cerebral cortex tissues. The mean malondialdehyde and COX-2 levels were statistically higher in the IR group; however, in the BIR group, these levels were within the normal limits. Furthermore, the mean total glutathione, COX-1 and SOD levels were markedly lower in the IR group, and within the normal limits in the BIR group. CONCLUSION: Benidipine hydrochloride may play a certain protective role in cerebral I/R injury. This effect may be related to improvement in the antioxidant capacity of brain tissue, and the inhibition of overproduction of inflammatory cytokines.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2021Valeriana officinalis is Effective on Stress-Induced Infertility, Delayed Maternity, and Intrauterine Growth Restriction of the Fetus in Female Rats
Uzel, Kemine; Turkler, Can; Mammadov, Renad; Yazici, Gulce Naz; Arslan, Yusuf Kemal; Coban, Taha Abdulkadir; Suleyman, Zeynep; Gursul, Cebrail; Suleyman, Halis
SUMMARY. Infertility is a tragic option for reproductive-aged women. About 20 % of lost ability to conceive are caused by unexplained etiology. Some of these cases are linked with psychological stress. It can cause depression and anxiety, and the latter can cause infertility. The therapy with sedative, antidepressant, anxi-olytic and antioxidant effects may be beneficial in preventing infertility caused by psychological disorders and maintaining normal intrauterine physical development of the fetus. The aim of our study is to investi-gate the protective effect of Valeriana plant root extract against stress-induced reproductive disorders. The protective effect of Valeriana plant root extract against infertility and delayed maternity was investigated in a stress model induced by the forced immobilization method in albino Wistar female rats and compared with ascorbic acid. Valeriana officinalis plant root extract was effective against psychological stress-related infertility and delayed maternity, ascorbic acid was found to be ineffective. This information suggests that psychological stress-related ovarian damage, infertility, delayed maternity, and great obstetric syndromes are based on the role of psychological disorders such as anxiety and depression rather than oxidative stress. Our experimental results indicate that Valeriana officinalis plant root extract can be useful in the treatment of stress-related ovarian damage and reproductive disorders.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2021The effect of lutein on ischemia-reperfusion-induced vasculitic neuropathic pain and neuropathy in rats
Yuceli, Sahin; Yazici, Gulce Naz; Mammadov, Renad; Suleyman, Halis; Ozdogan, Selcuk
Background/Aim: Neuropathic pain and neuropathy is commonly seen after ischemia-reperfusion injuries. Our aim was to evaluate the effect of lutein on ischemia-reperfusion (I/R)- induced vasculitic neuropathic pain and neuropathy in rats. Materials and Methods: An hour before anesthesia, 6 Albino Wistar male rats with I/R were orally administered with 1 mg/kg lutein (LIR group). Two groups of 6 such rats who underwent surgery were provided with 0.5 ml distilled water (as solvent) either via oral administration (SIR group) or by gavage (sham group or SG). One hour following the administration, the later femoral arteries of the LIR and SIR rats were closed using a sterile silk thread and ischemia was induced in the sciatic nerve for 4 h, followed by reperfusion for 24 h. The femoral artery of the SG group was not closed with suture. Next, 1 mg/kg lutein was re-administered only to the LIR group for 1 h, followed by measurement of the paw pain thresholds by the Basile Algesimeter. The levels of malondialdehyde (MDA), total glutathione (tGSH), nuclear factor-kB (NF-κB), and tumor necrosis factor-alpha (TNF-α) in the sciatic nerve tissues were measured, and the tissues were histopathologically examined. Results: We found that the MDA, NF-κB, and TNF-α levels were higher and the tGSH level was lower in the SIR group relative to those in the LIR group, and the differences were statistically significant. Significant histopathological damage was noted in the SIR group, whereas the LIR group demonstrated protection from oxidative damage. Conclusion: Lutein is potentially useful in the treatment of I/R-related neuropathy and neuropathic pain.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2021Protective effect of lutein against acrolein-induced ototoxicity in rats
Erhan, Ertugrul; Salcan, Ismail; Bayram, Rana; Suleyman, Bahadir; Dilber, Muhammet; Yazici, Gulce Naz; Coban, Taha Abdulkadir; Altuner, Durdu; Suleyman, Halis
Background: Acrolein is a reactive aldehyde that forms during burning of wood and other fuels. It is also a product of lipid peroxidation (LPO) reactions and is present in cigarette smoke. Acrolein is known to cause oxidative stress and inflammatory nerve tissue damage. Lutein is a tetraterpenoid molecule with antioxidant and anti-inflammatory properties. There appear to be no studies on the effect of lutein on vestibulocochlear nerve damage induced by acrolein. The aim of this study was to investigate the effect of lutein on vestibulocochlear nerve damage induced by acrolein in rats using biochemical and histopathological methods. Methods: The rats were divided into three groups (n = 6, for each group) a healthy control group (HG), an acrolein (ACR) group and a lutein and acrolein (LACR) group. In the LACR group, lutein was administered (1 mg/kg) via oral gavage. The ACR and HG groups received saline via oral gavage. Then, 1 h after the administration of lutein and saline, the LACR and ACR groups were treated with 3 mg/kg of acrolein via oral gavage. This procedure was repeated once a day for 30 days. Results: The results of biochemical experiments showed that in the vestibulocochlear nerve tissues of the animals treated with acrolein, the levels of malondialdehyde, total oxidants, nuclear factor kappa b, tumor necrosis factor alpha and interleukin 1 beta significantly increased, whereas the levels of total glutathione and total antioxidants decreased as compared to those in the HG and LACR groups. In addition, severe histopathological damage was observed in vestibulocochlear nerve tissue of the acrolein group, whereas this damage was alleviated in the lutein group. Conclusion: Lutein protected vestibulocochlear nerve tissue from acrolein-associated oxidative and proinflammatory damage. This suggests that lutein might be useful in preventing or treating acrolein-induced ototoxicity.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2021Coenzyme Q10 effect on cisplatin-induced oxidative retinal injury in rats
Sunar, Mukadder; Yazici, Gulce Naz; Mammadov, Renad; Kurt, Nezahat; Arslan, Yusuf Kemal; Süleyman, Halis
Aim: In this study, it was aimed to investigate the effect of coenzyme Q10 (CoQ10) on cisplatin-induced oxidative retinal damage in rats biochemically and histopathologically. Materials and methods: Thirty male Wistar albino rats were divided into 3 groups randomly: untreated control (C group), only 2.5 mg/kg cisplatin daily administrated group for 2 weeks (CP group), 2.5 mg/kg cisplatin + 20 mg/kg orally CoQ10 daily administrated group for 2 weeks (CoQC group). At the end of experimental period, blood samples obtained before sacrification for the biochemical examination of serum malondialdehyde (MDA), total glutathione (tGSH), total oxidant system (TOS), total antioxidant systemic (TAS) levels and after eyes were removed for examined histopathology. Results: As a result of our study, severe histopathological damage was detected in the retinal tissue of the cisplatin group with serum malondialdehyde (MDA) and total oxidant system (TOS) levels were high and total glutathione (tGSH) and total antioxidant systemic (TAS) levels were low. However, it was observed that the histopathological damage associated with cisplatin was decreased in the retinal tissue of the CoQ10 group, which inhibited the increase in blood serum MDA/TOS levels and decrease in tGSH/TAS levels. Conclusion: The biochemical and histopathological results of our study were compatible with each other, so we concluded that the damage to the rat retinal tissue caused by cisplatin may be reversible with coenzyme.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2021Effect of Taxifolin on Ischemia/Reperfusion-Induced Oxidative Injury of Sciatic Nerve in Rats
Yuceli, Sahin; Suleyman, Bahadir; Yazici, Gulce Naz; Mammadov, Renad; Cankaya, Murat; Kunak, Celaleddin Semih; Bulut, Seval; Suleyman, Halis; Altuner, Durdu
Background: Ischemia is a condition in which blood flow to tissues is decreased or entirely stopped for various reasons. The reperfusion process exacerbates damage caused by ischemia in the organs and tissues. Reactive oxygen species (ROS) are mainly responsible for ischemia-reperfusion (IR) damage. ROS increase results in lipid peroxidation (LPO) and oxidative stress. In the literature, taxifolin reportedly suppresses ROS production. This study aimed to determine the effect of taxifolin, which is a flavonoid, on IR injury of the sciatic nerve in rats. Methods: This study divided 30 albino Wistar rats into 3 groups: IR without medication (IR) group, taxifolin applied IR (TAX+IR) group, and only dissection made to the sciatic nerve sham group (SHAM). Sciatic nerve injury was induced by applying 2 hours of ischemia and 3 hours of reperfusion to the abdominal aorta and iliolumbar arteries. Biochemical and histopathologic investigations then were performed on sciatic nerve tissues. Malondialdehyde, total glutathione, glutathione reductase, and glutathione peroxidase were analyzed as oxidative stress markers, and tumor necrosis factor–α and interleukin-1β levels were evaluated as inflammatory stress markers in biochemical tests. Results: The IR group has statistically significantly high oxidant and cytokine levels and low antioxidant levels compared with the TAX+IR group. Taxifolin treatment was also shown to cause significant histopathologic improvement. Conclusions: We suggest that taxifolin may be effective in preventing IR injury of the sciatic nerve.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2021The Effect of Taxifolin on Acrylamide-induced Oxidative and Proinflammatory Brain Injury in Rats: A Biochemical and Histopathological Study
Ersoy, Alevtina; Yasar, Hasan; Tanoglu, Ceyda; Yazici, Gulce Naz; Coban, Taha Abdulkadir; Arslan, Yusuf Kemal; Suleyman, Halis
Purpose: Acrylamide is a well-known environmental toxic compound. Taxifolin belongs to the group of flavonoids that have antioxidant, antimicrobial, anti-inflammatory and anti-carcinogenic properties. In this study, we investigated the effect of taxifolin on acrylamide-related oxidative and proinflammatory brain damage. Methods: The experimental animals were divided into three groups: (1) those treated with acrylamide 20 mg/kg p.o., (2) those treated with taxifolin 50 mg/kg p.o. and acrylamide and (3) the control group. At the end of the experiment, the rat brain tissues were examined for the levels of Malondialdehyde (MDA), total glutathione (tGSH), tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β). A histopathological analysis was performed to detect the morphological changes in the brain. Results: Exposure to acrylamide caused a significant increase in the levels of MDA, TNF-α and IL-1β and a decrease in the values of tGSH, which indicates the presence of oxidative stress and inflammation in the brain tissue. Taxifolin treatment significantly reduced the levels of MDA and TNF-α and brought the mean values of IL-1β and tGSH close to those of the control group. The group that received acrylamide exhibited histopathological changes, such as neuronal degeneration, edema of microglia, dilated and congestive vessels and apoptotic inclusion. The use of taxifolin significantly improved the morphological changes in the brain tissue of the acrylamide-exposed rats. Conclusion: Acrylamide causes brain damage, inducing oxidative stress and inflammation. Due to its antioxidant and anti-inflammatory properties, taxifolin may be one of the agents that can reduce the neurotoxic effects of acrylamide.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2021Effect of Sunitinib on Liver Oxidative and Proinflammatory Damage Induced by Ischemia-Reperfusion in Rats
Bilici, Sami; Yazici, Gulce Naz; Altuner, Durdu; Aggul, Ahmet Gokhan; Suleyman, Halis
Background: Ischemia-reperfusion (IR) injury is defined as a complex pathologic process that begins with the oxygen deprivation of tissue, continues with the production of reactive oxygen radicals (ROS), and expands with an inflammatory response. This study investigates the protective effects of sunitinib, an anticancer drug with demonstrated antioxidant and anti-inflammatory activity, against liver IR damage. Our study aims to investigate the biochemical and histopathologic effects of sunitinib on IR-induced liver damage in rats. Methods: Albino Wistar male rats were divided into 3 groups: liver IR control (IR), 25 mg/kg sunitinib + liver IR (S+IR), and sham operation (SHAM). Results: In the liver tissue of the IR group, oxidant and proinflammatory cytokine levels such as malondialdehyde, nuclear factor κ B, tumor necrosis factor-α, and interleukin-1β increased compared with the SHAM and S+IR groups. In addition, antioxidant levels such as total glutathione, glutathione reductase, and glutathione peroxidase were found to be significantly lower in the IR group than in the SHAM and S+IR groups. Although severe histopathologic damage was observed in the IR group, it was evaluated as mild in the S+IR group. The results obtained suggest that sunitinib may be helpful in the treatment of liver IR injury.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2020The effect of lutein on paclitaxel-induced neuropathy and neuropathic pain in rats
Yasar, Hasan; Ersoy, Alevtina; Yazici, Gulce Naz; Suleyman, Halis; Özgeris, Fatma Betul; Arslan, Yusuf Kemal; Sunar, Mukadder
Background: Paclitaxel is a drug used to treat cancer, but it causes neuropathic pain and peripheral neuropathy. One of the causes of these effects of paclitaxel on nerve tissue is oxidative damage. Lutein has antioxidant effects. In this study, it was investigated the influence of lutein whether decrease oxidative damage of paclitaxel on nerve tissue or not. Methods: Pain threshold (PT) in paw of paclitaxel-only group (PC), paclitaxel+lutein group (PL), and control group (CG) rats were measured using a Basile algesimeter. The sciatic nerve tissue of experimental animals was used to conduct biochemical analyses. Analyses of malondialdehyde (MAD), total glutathione (GT), myeloperoxidase (MP), interleukin 1 beta (IL-1 beta) were performed. Histopathologic examination was performed in all groups. The CG and the other groups were compared. Results: PT was lowest in the PC group. However considerable increase in pain threshold in paw was observed in the PL group with respect to the PC group. As a result, the usage of lutein increased PT in paw. The PC group had signs of oxidative damage. With the use of lutein, paclitaxel was prevented from causing oxidative damage. Histopathological examination revealed that paclitaxel caused damage to myelin sheath and axon of sciatic nerve tissue. However, lutein was found to prevent this damage to the myelin sheath and axon. Paclitaxel causes neuropathic pain and peripheral neuropathy. One of the causes is the oxidative damage of paclitaxel in nerve tissue. Conclusion: Lutein, which has an antioxidant effect, prevents the damage of paclitaxel on nerve tissue.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2020Effect of Taxifolin on Cobalt-induced Ototoxicity in Rats: A Biochemical and Histopathological Study
Salcan, Ismail; Dilber, Muhammet; Bayram, Rana; Suleyman, Esma; Erhan, Ertugrul; Yi, Sevil Karahan; Yazici, Gulce Naz; Coban, Abdulkadir; Suleyman, Halis
Background and Objective: Cobalt is a metal with a significant hazard profile, used widely in many industries. The reported hazards of Cobalt include effects on the heart, thyroid, immune system, vision and hearing organs as a result of exposure to high doses. This study aimed to evaluate the biochemical and histopathological effect of taxifolin on Co-induced ototoxicity in rats. Materials and Methods: Distilled water was administered to the Healthy Group (HG) and cobalt-only (Co) groups of animals and a third group was orally given 50 mg kg(-1) Taxifolin+Cobalt (TCo) by gavage. After 1 hr, 150 mg kg(-1) CoCl2 was administered orally to the stomach by gavage to the animals in the TCo and the Co groups. This procedure was repeated once a day for seven days. Results: Malondialdehyde (MDA), Nuclear Factor-Kappa B (NF-kappa B), tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) levels were significantly higher than the HG and TCo groups and the cochlear nerve tissue was damaged by significant histopathological damage in the Co group with low total glutathione (tGSH) levels. In the TCo group, biochemical and histopathological findings were found to be close to the HG group. Conclusion: This study revealed the role of oxidative stress and proinflammatory cytokines in the pathogenesis of Co ototoxicity. Our results suggest that taxifolin may be useful in the treatment of Co-related ototoxicity.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2020The effect of rutin on experimental traumatic brain injury and edema in rats
Yuceli, Sahin; Yazici, Gulce Naz; Mammadov, Renad; Suleyman, Halis; Kaya, Mustafa; Ozdogan, Selcuk
Background/Aim: The aim of the study was to evaluate the effect of rutin, which is a vitamin P1 flavonoid with anti-inflammatory and anti-edema effects, on traumatic brain injury (TBI) and edema in rats. Materials and Methods: Rats were divided into 3 groups as sham group without brain trauma (SG), brain trauma without medication (BT) group and Rutin treated brain trauma (RBT) group. Fifty mg/kg rutin was administered to the RBT group once a day for three days. On the fourth day, rats were sacrificed. Extracted brain tissues were examined biochemically and histopathologically. Results: We found that the levels of malondialdehyde, nuclear factorkappa B and tumor necrosis factor-alpha decreased, and those of total glutathione increased significantly. Furthermore, rutin administration reduced pyramidal neuron degeneration and poly-morpho-nuclear-leucocyte accumulation due to trauma in brain tissue, while eliminating edema. Conclusion: Rutin might be effective in the treatment of TBI and TBI-related brain edema.
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