HPLC Analysis of Phenolic Compounds from Gypsophila aucheri Boiss. and Investigation of Antioxidant and Cytotoxic Activity of Gypsophila aucheri Boiss. exracts

This study was designed to investigate the antioxidant and antiproliferative activities of Gypsophila aucheriBoiss. extracts as well as phenolic content by RP-HPLC technique. Antioxidant activity potentials of theextracts were evaluated by four different methods namely, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging capacity tests, cupric ionreducing antioxidant capacity (CUPRAC) method, and metal chelating assay. Antiproliferative activities of theextracts were tested against breast carcinoma (MCF-7), colorectal carcinoma (HT-29), and hepatocellularcarcinoma (HepG2) cells. RP-HPLC analysis revealed the presence of eight phenolic compounds in eighteenphenolics scanned. Antioxidant activity results showed that methanol extract of Gypsophila aucheri displayedmore pronounced antioxidant activity than water extract according to DPPH and ABTS tests, whereas waterextract was superior than methanol extract for CUPRAC and metal chelating activity tests. Cytotoxicity testsdemostrated the promising antiproliferative activity of methanol extract against all the tested cancer cell lineswith its lower IC50 values. However, both extracts were observed to display moderate biological activitycompared to positive controls. The obtained data suggest that Gypsophila aucheri could be evaluated as apromising source for food and nutraceutical industries due to its striking antioxidant and moderateantiproliferative potentials together with high phytochemical profile.