Sürdürülebilir Kalkınma Amaçları
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- ArticleAçık Erişim2021Are the body shape index, the body roundness index and waist-to-hip ratio better than BMI to predict recurrent pregnancy loss?
Kiremitli, Tunay; Kiremitli, Sevil; Ulug, Pasa; Dinc, Kemal; Uzel, Kemine; Arslan, Yusuf Kemal
Purpose: Etiology could not be determined in approximately 50% of recurrent pregnancy loss cases, and it was named unexpected recurrent pregnancy loss(URPL). A body shape index(ABSI), body roundness index(BRI), and waist-to-hip ratio(WtHR) are new indexes that are superior to BMI in showing body fat distribution. We aimed to investigate the potency of ABSI, BRI, and WtHR in URPL, their superiority to BMI, and their suitability for clinical use. Methods: One hundred and thirty-eight patients between the ages of 20-40 who applied to our hospital for URPL between January 2016 and December 31, 2020 were included in our study. Weight, height, waist circumference, and hip circumference were measured, and indexes were calculated. Differences between the URPL and control groups were calculated using the IBM SPSS program. Results: There was a significant difference between the two groups for BRI, ABSI, and WtHR values, while there was no significant difference in BMI. BRI(4.4 ± 1.7vs3.9 ± 1.5), ABSI(0.08 ± 0.005 vs 0.078 ± 0.004), and WtHR(0.84 ± 0.06vs0.82 ± 0.05) values were higher in the URPL group. ROC analysis showed us that BRI, ABSI, and WtHR have a diagnostic value for URPL(P <.05). When indexes were above the cutoff values, RPL risk increased 3.59 times in ABSI, 2.26 times in BRI, and 2.9 times in WtHR(P <.05). Conclusions: The relationship between obesity and URPL can be explained more clearly by using effective indexes that show body fat distribution rather than BMI. Ethics committee approval was obtained from Erzincan Binali Yildirim University in 14.01.2021. Clinical Research Ethics Committee no: 01/01.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2021Effect of clival bone growth on the localization of the abducens nerve at the petroclival region: a postmortem anatomical study
Kayacı, Selim; Ozveren, Mehmet Faik; Bas, Orhan; Ayberk, Giyas; Aslan, Murat Nihat; Sam, Bulent; Arslan, Yusuf Kemal
Purpose: To investigate the effect of the clival bone pattern on the abducens nerve (AN) localization in the petroclival region between the Pediatric and Adult Groups. Methods: This study used 12 pediatric and 17 adult heads obtained from the autopsy. The length and width of the clivus and the length of the petrosphenoidal ligaments (PSLs) were measured. The ratio of the length and width of the clivus was accepted as the clival index (CI). The localization of the AN at the petroclival region below the PSL, classified as lateral and medial, were recorded. Results: The average length of the clivus was 26.92 ± 2.88 mm in the Pediatric Group, and 40.66 ± 4.17 mm in the Adult Group (p < 0.001). The average width of the clivus was 22.35 ± 2.88 mm in the Pediatric Group, and 29.96 ± 3.86 mm in the Adult Group (p < 0.001). The average value of the CI was 1.20 in the Pediatric Group and 1.36 in the Adult Group (p = 0.003). The length of the PSL was 7.0 ± 1.47 mm in the Pediatric Group and 11.05 ± 2.95 mm in the Adult Group (p < 0.001). The nerve was located below the medial side of the PSL in the Pediatric Group and below the lateral side in the Adult Group (p = 0.002). Conclusions: The petrous apex localization of the AN in adults compared with pediatric subjects could be related to the increased growth in the length of the clivus than its width.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2021Coenzyme Q10 effect on cisplatin-induced oxidative retinal injury in rats
Sunar, Mukadder; Yazici, Gulce Naz; Mammadov, Renad; Kurt, Nezahat; Arslan, Yusuf Kemal; Süleyman, Halis
Aim: In this study, it was aimed to investigate the effect of coenzyme Q10 (CoQ10) on cisplatin-induced oxidative retinal damage in rats biochemically and histopathologically. Materials and methods: Thirty male Wistar albino rats were divided into 3 groups randomly: untreated control (C group), only 2.5 mg/kg cisplatin daily administrated group for 2 weeks (CP group), 2.5 mg/kg cisplatin + 20 mg/kg orally CoQ10 daily administrated group for 2 weeks (CoQC group). At the end of experimental period, blood samples obtained before sacrification for the biochemical examination of serum malondialdehyde (MDA), total glutathione (tGSH), total oxidant system (TOS), total antioxidant systemic (TAS) levels and after eyes were removed for examined histopathology. Results: As a result of our study, severe histopathological damage was detected in the retinal tissue of the cisplatin group with serum malondialdehyde (MDA) and total oxidant system (TOS) levels were high and total glutathione (tGSH) and total antioxidant systemic (TAS) levels were low. However, it was observed that the histopathological damage associated with cisplatin was decreased in the retinal tissue of the CoQ10 group, which inhibited the increase in blood serum MDA/TOS levels and decrease in tGSH/TAS levels. Conclusion: The biochemical and histopathological results of our study were compatible with each other, so we concluded that the damage to the rat retinal tissue caused by cisplatin may be reversible with coenzyme.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2021The Effect of Taxifolin on Acrylamide-induced Oxidative and Proinflammatory Brain Injury in Rats: A Biochemical and Histopathological Study
Ersoy, Alevtina; Yasar, Hasan; Tanoglu, Ceyda; Yazici, Gulce Naz; Coban, Taha Abdulkadir; Arslan, Yusuf Kemal; Suleyman, Halis
Purpose: Acrylamide is a well-known environmental toxic compound. Taxifolin belongs to the group of flavonoids that have antioxidant, antimicrobial, anti-inflammatory and anti-carcinogenic properties. In this study, we investigated the effect of taxifolin on acrylamide-related oxidative and proinflammatory brain damage. Methods: The experimental animals were divided into three groups: (1) those treated with acrylamide 20 mg/kg p.o., (2) those treated with taxifolin 50 mg/kg p.o. and acrylamide and (3) the control group. At the end of the experiment, the rat brain tissues were examined for the levels of Malondialdehyde (MDA), total glutathione (tGSH), tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β). A histopathological analysis was performed to detect the morphological changes in the brain. Results: Exposure to acrylamide caused a significant increase in the levels of MDA, TNF-α and IL-1β and a decrease in the values of tGSH, which indicates the presence of oxidative stress and inflammation in the brain tissue. Taxifolin treatment significantly reduced the levels of MDA and TNF-α and brought the mean values of IL-1β and tGSH close to those of the control group. The group that received acrylamide exhibited histopathological changes, such as neuronal degeneration, edema of microglia, dilated and congestive vessels and apoptotic inclusion. The use of taxifolin significantly improved the morphological changes in the brain tissue of the acrylamide-exposed rats. Conclusion: Acrylamide causes brain damage, inducing oxidative stress and inflammation. Due to its antioxidant and anti-inflammatory properties, taxifolin may be one of the agents that can reduce the neurotoxic effects of acrylamide.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2020Genotype distribution in Hepatitis C patients admitted to Erzincan Mengücek Gazi Training and Research Hospital
Karakeçili, Faruk; Arslan, Yusuf Kemal; Yılmaz, Aysun; Kara, Murat; Dabanlıoğlu, Bülent
Aim: We aimed to determine the genotype distribution in Hepatitis C patients who Parasitology applied to Erzincan Binali YildirimUniversity, Mengücek Gazi Training and Research Hospital. Hepatitis C virus, the only member of the genus Hepacivirus of Flaviviridaefamily, is responsible for 25%-40% of all liver diseases. Hepatitis C virus causes acute hepatitis C and chronic hepatitis C infections.Chronic hepatitis C infection causes cirrhosis, liver failure, hepatocellular cancerand liver diseases in terminal periods.Material and Methods: A total of 77 patients, 41 male and 36 female, who were admitted to Erzincan Binali Yıldırım University,Mengücek Gazi Training and Research Hospital in the period of January 2013-June 2019, were included in our study. RT-PCR andDNA sequencing for the 5’UTR region of the HCV genome for HCV genotyping was performed using the ABI Prism 3130 × 1 DNASequencer device.Results: Sex of a total of 77 patients included in the study; 41 of them are men (53.2%) and 36 are women (46.8%). Average age of thepatients; It was 59.9 ± 16.6 (minimum 20 and maximum 94). HCV G1b (80.5%) in 62 patients, HCV G1a (7.8%) in 6, HCV G3a (3.9%)in 3, HCV G3 (2.6%) in 2 and HCV G2 (2.6%) was found in 2 patients. HCV G1 (1.3%) was detected in 1, HCV G4 (1.3%) in 1 patient.Conclusion: The dominant genotype in Hepatitis C patients who applied to Mengücek Gazi Training and Research Hospital wasdetermined as “HCV Genotype 1b”.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2020The effect of lutein on paclitaxel-induced neuropathy and neuropathic pain in rats
Yasar, Hasan; Ersoy, Alevtina; Yazici, Gulce Naz; Suleyman, Halis; Özgeris, Fatma Betul; Arslan, Yusuf Kemal; Sunar, Mukadder
Background: Paclitaxel is a drug used to treat cancer, but it causes neuropathic pain and peripheral neuropathy. One of the causes of these effects of paclitaxel on nerve tissue is oxidative damage. Lutein has antioxidant effects. In this study, it was investigated the influence of lutein whether decrease oxidative damage of paclitaxel on nerve tissue or not. Methods: Pain threshold (PT) in paw of paclitaxel-only group (PC), paclitaxel+lutein group (PL), and control group (CG) rats were measured using a Basile algesimeter. The sciatic nerve tissue of experimental animals was used to conduct biochemical analyses. Analyses of malondialdehyde (MAD), total glutathione (GT), myeloperoxidase (MP), interleukin 1 beta (IL-1 beta) were performed. Histopathologic examination was performed in all groups. The CG and the other groups were compared. Results: PT was lowest in the PC group. However considerable increase in pain threshold in paw was observed in the PL group with respect to the PC group. As a result, the usage of lutein increased PT in paw. The PC group had signs of oxidative damage. With the use of lutein, paclitaxel was prevented from causing oxidative damage. Histopathological examination revealed that paclitaxel caused damage to myelin sheath and axon of sciatic nerve tissue. However, lutein was found to prevent this damage to the myelin sheath and axon. Paclitaxel causes neuropathic pain and peripheral neuropathy. One of the causes is the oxidative damage of paclitaxel in nerve tissue. Conclusion: Lutein, which has an antioxidant effect, prevents the damage of paclitaxel on nerve tissue.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2020Early cranioplasty associated with a lower rate of post-traumatic hydrocephalus after decompressive craniectomy for traumatic brain injury
Ozoner, Baris; Kilic, Mustafa; Aydin, Levent; Aydin, Seckin; Arslan, Yusuf Kemal; Musluman, Ahmet Murat; Yilmaz, Adem
Background: Post-traumatic hydrocephalus (PTH) is one of the primary complications during the course of traumatic brain injury (TBI). The aim of this study was to define factors associated with the development of PTH in patients who underwent unilateral decompressive craniectomy (DC) for TBI. Methods: A total of 126 patients, who met the inclusion criteria of the study, were divided into two groups: patients with PTH (n = 25) and patients without PTH (n = 101). Their demographic, clinical, radiological, operative, and postoperative factors, which may be associated with the development of PTH, were compared. Results: Multivariate logistic regression analysis revealed that cranioplasty performed later than 2 months following DC was significantly associated with the requirement for ventriculoperitoneal shunting due to PTH (p < 0.001). Also, a significant unfavorable outcome rate was observed in patients with PTH at 1-year follow-up according to the Glasgow Outcome Scale-Extended (p = 0.047). Conclusions: Our results show that early cranioplasty within 2 months after DC was associated with a lower rate of PTH development after TBI.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2020Vitamin e and Selenium Reduce Prednisolone Side Effects in Rat Hearts
Bardas, Ebru; Arslan, Yusuf Kemal; Polat, Seher; Erisir, Mine; Uslu, Gözde Atila; Cetin, Nihal; Cicek, Betul
Aim of this work was to determine the effects of dietary intake vitamin E and Se on lipid peroxidation (LPO) as Thiobarbituric acid reactive substances (TBARS) and on the antioxidative defense mechanisms in heart tissues of rats treated with high doses of prednisolone. 250 adult male Wistar rats were randomly divided into 5 groups and fed with normal diet. Additionally groups 3, 4, and 5 received a daily supplement in their drinking water of 20 mg vitamin E, 0.3 mg Se, and a combination of vitamin E and Se (20 mg/0.3 mg), respectively, for 30 days. For 3 d subsequently, control group was treated with placebo, and remaining four groups were injected intramuscularly with 100 mg/kg prednisolone. After last administration of prednisolone, 10 rats from each group were killed at 4, 8, 12, 24, and 48 h and the activities of antioxidant enzymes and the levels of GSH and TBARS were measured. GSH-Px, CAT activities and GSH levels decreased starting from 4th hour to 48% and 65% of control levels by 24th hour, respectively and it reincreased to control levels at 48th hour in the prednisolone group (p < 0.001, p < 0.001). In addition, prednisolone administration led 2-fold increase in heart TBARS levels at 24th hour (p < 0.001). E vitamins and Se inhibited the increase in heart TBARS and the decrease in antioxidative enzymes levels. Therefore, It is concluded that vitamin E and Se may have a preventive role in decreasing the increase of TBARS caused by prednisolone administration in our study.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2020Evaluating the relationship between the pleth variability index and hypotension and assessing the fluid response in geriatric hip fracture under spinal anaesthesia: An observational study
Küpeli, İlke; Subaşı, Faruk; Eren, Nurhan; Arslan, Yusuf Kemal
Objective: We aimed to test the efficacy of the pre-operative Pleth variability index (PVI) in evaluating hypotension that developed after spinal anaesthesia in patients who were spontaneously breathing, pre-operatively hypovolemic, and were at an advanced age. Methods: This observational study included 94 patients aged >65 years with hip fracture. Demographic data, pre-operative heart rate, non-in-vasive arterial pressures, PVI values, and haemogram values were continuously measured following spinal anaesthesia. The measurements with and without hypotension were distinguished and their data were compared. Results: The mean age of the patients was 77.4±8.2 years. In total, 56.4% of the patients developed hypotension after spinal anaesthesia, and hypotension was higher in women (p=0.037). Low pre-operative diastolic arterial pressures values were associated with the development of hy-potension (p=0.037). The relationship between PVI and post-spinal hypotension was negative but significant (r=−0.239; p<0.05). Depending on the volume loss, an increase in the PVI (p<0.001) and its subsequent significant decrease after treatment in patients with hypotension (p<0.001) was observed. The correlation between noninvasively measured haemoglobin values and the values obtained from arterial blood gas samples was significant (p<0.001). Conclusion: This study showed that post-spinal hypotension may be associated with increased as well as decreased PVI values. However, these values cannot be clinically used for predicting pre-operative hypotension in hypovolemic patients.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2020Comparison of thalamus volume on magnetic resonance and cadaveric section images
Kayaci, Selim; Bas, Orhan; Celiker, Fatma Beyazal; Ucuncu, Yilmaz; Arslan, Yusuf Kemal; Ozveren, Mehmet Faik; Aykol, Sukru
AIM: To measure and to compare the volume of thalamus using magnetic resonance imaging (MRI) and the anatomical sections. MATERIAL and METHODS: In this study, 13 brain specimens were used. First, the images were taken in 3 mm sections on MRI, the thickness of the thalamus was measured. Subsequently, 4 mm coronal sections were prepared using a microtome. The thalamic volumes calculated from cadaveric specimens were compared with the measurements obtained using MRI. RESULTS: On MRI, the mean thalamic volumes on the right and left hemispheres were found to be 5843.4 ± 361.6 mm3 and 5377.0 ± 666.2 mm3 respectively. The mean volumes of the cadaveric sections were 5610.8 ± 401.3 mm3 on the right side and 5618.5 ± 604.1 mm3 on the left hemisphere. No statistically significant difference was found between the volume calculated from MRI and that obtained from the cadaveric section (p<0.05). CONCLUSION: This study shows a correlation between measurement of thalamus volume based on MRI and those calculated from anatomical sections. Our findings support the reliability of DBS procedures using MRI and stereotactic method.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2019The findings of corneal specular microscopy in patients with keratoconus
Arslan, Yusuf Kemal; Agcayazi, Sumeyye Burcu; Ucak, Turgay; Uğurlu, Adem; İçel, Erel; Taşlı, Nurdan Gamze; Karakurt, Yucel
Aim: The aim of this study was to determine the correlation of corneal endothelial cell density, coefficient of variation and hexagonal cell ratio with the stages of keratoconus and corneal thickness.Material and Methods: The study included 102 eyes from 51 patients. The corneal topography was performed using the Sirius corneal topographic device and the examination of the endothelium was performed using Nidek CEM-530. The patients were divided into groups in accordance with keratometry values (group 1: K2<45 D, group 2: K2 45-52 D, group 3: K2> 52 D) and thinnest cornea thickness (group 1:> 450 μm, group 2: 400-450 μm, group 3: <400 μm). Specular microscopic findings were compared according to the severity of keratoconus and corneal thickness.Results: Of the patients, 29 (50%) were male and 22 (50%) were female. The mean age was 29.8 ± 7.7 (15-45) years. Endothelial cell count and hexagonal cell ratio were significantly lower in severe keratoconus compared to mild and moderate keratoconus and the coefficient of variation was higher in severe keratoconus compared to the other groups (p <0.001). When the patients were compared according to the pachymetry value at the thinnest point of the cornea, the endothelial cell number and the hexagonal cell ratio were found to be lower in the group with pachymetry less than 400 μm (p <0.001). The coefficient of variation was higher in the same group compared to the others (p <0.001).Conclusion: It was revealed that endothelial cell density, hexagonal cell ratio decreased and the coefficient of variation increased with the progression of keratoconus. Specular microscopic examinations in patients with keratoconus provide important data in addition to topography.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2019Frequency of Rota Virus in Children with Acute Gastroenteritis
Karakeçili, Faruk; Topal, İsmail; Arslan, Yusuf Kemal; Aydin Peker, Necla; Sürücü Kara, İlknur; Çıkman, Aytekin
Objective: Gastroenteritis is a common clinical condition in infancy and childhood. It is reported that 17% of the deaths in children under the age of five in the world are caused by diarrheal diseases. In this age group, the agents in 70% of acute gastroenteritis cases are active viruses. Rotavirus is the most frequent viral factor. In the study it was aimed to determine the frequency of rotavirus in pediatric patients who applied to Erzincan Mengücek Gazi Education and Research Hospital with complaints of diarrhea. Material and Method: Between January 2013 and December 2016, the results of stool samples of 7851 patients screened for rotavirus antigen were evaluated retrospectively. During these periods, the presence of rotavirus antigen was investigated by immunochromatographic method in fresh stool specimens. Results: A total of 4416 (56%) stool samples were obtained from males, and 3435 (44%) were obtained from females. Analysis revealed that 820 (10.44%) of the specimens were rotavirus antigen positive. Rotavirus positivity was most commonly seen in the age group from 2 to 5 years in winter and spring. Conclusion: Rotavirus infections still maintain their importance and cause seasonal epidemics. Rotavirus vaccine might be taken into consideration for the national vaccination program, because of the common frequency in the age group from 2 to 5 years.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2019Bruselloz Hastalarında Asimetrik Dimetilarjinin (ADMA) Düzeylerinin Araştırılması
Terzioğlu, Merve Aydın; Karakeçili, Faruk; Arslan, Yusuf Kemal; Sezgin, Muhammed; Gülhan, Barış; Çıkman, Aytekin
Amaç: Asimetrik dimetilarginin (ADMA), nitrik oksit sentazın (NOS) ana endojen inhibitörüdür. NOS, konak savunmasında vevasküler yapının sürdürülmesinde önemli rol oynamaktadır. Yüksek ADMA düzeylerinin endoteldis fonksiyonu ile ilişkili olduğu veçeşitli hastalıklarda rol aldığı gösterilmiştir. Brucellaspp.’nin neden olduğu zoonoz bir hastalık olan bruselloz, vaskülopati olarakkendini gösterebilmektedir. Ancak, bruselloz ile ADMA arasındaki ilişki ile ilgili yeterli veri bulunmamaktadır. Çalışmamızın amacıbruselloz hastalarında ve kontrol grubunda ADMA düzeyinin araştırılmasıdır.Yöntemler: Çalışmamıza 40 akut bruselloz hastası ve 40 sağlıklı gönüllü alındı. Bruselloz hastalarında ve sağlıklı gönüllülerdeserum ADMA düzeyleri İnsan ADMA ELISA kiti ile üretici firma önerileri doğrultusunda araştırıldı. Katılımcıların fiziki muayeneleriyapılarak, cinsiyet, yaş ve ikamet adreslerini içeren demografik karakteristikleri ve laboratuvar test sonuçları kayıt altına alındı.Verilerin değerlendirilmesinde IBM SPSS 20 istatistik paket programı kullanıldı. İstatistiksel anlamlılık düzeyi (p<0.05) ve (p<0.01)olarak kabul edildi.Bulgular: Çalışmaya alınan 40 akut brusellozlu hastanın 18'i (% 45) erkek, 22'si (% 55) kadındı ve yaş ortalaması 49.22 ± 17.33 idi.Kontrol grubundaki 40 sağlıklı gönüllünün 18'i (% 45) erkek, 22'si (% 55) kadındı ve yaş ortalaması 39.02 ± 7.88 idi. Serum ADMAdüzeyleri bruselloz ve kontrol grubunda sırasıyla; 262.6±139.59 ng/ml ve 196.52±85.25 ng/ml olarak saptandı. Brusellozgrubunda, kontrol grubu ile karşılaştırıldığında istatistiksel olarak anlamlı bir farklılık tespit edildi (p=0.013).Sonuç: Çalışmamızda bruselloz grubundaki hastalarda serum ADMA düzeyleri, kontrol grubuna göre anlamlı olarak yüksekbulundu. ADMA düzeylerindeki bu yüksekliğin, brusellozun vaskülitteki rolü konusunda yeni yorumlar yapılmasına olanaksağlanacağı ve yeni tedavi yöntemlerinin gündeme gelebileceği kanaatindeyiz.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2019Peripheral neurotoxic effects of cisplatin on rats and treatment with rutin
Yaşar, Hasan; Ersoy, Alevtina; Cimen, Ferda Keskin; Mammadov, Renad; Kurt, Nezahat; Arslan, Yusuf Kemal
Background. Cisplatin, used in cancer treatment, has toxic and apoptotic effects on the peripheral nervous system. Rutin, also known as vitamin P, has antioxidant and antiapoptotic activity. Objectives. The purpose of this study was to investigate the biochemical and histopathologic efficacy of rutin on neurotoxic and apoptotic effects caused by cisplatin in the peripheral nervous system. Material and methods. Twenty-four albino Wistar male rats were divided into the following 4 groups: control group (CG), only cisplatin-injected group (CIS), cisplatin and rutin 50 mg/kg (RG-50)-injected group, and cisplatin and rutin 100 mg/kg (RG-100)-injected group. Analyses were performed on sciatic nerve tissue of experimental animals. Analyses of malondialdehyde (MDA), total glutathione (tGSH), glutathione reductase (GSHRd), glutathione-s-transferase (GST), and superoxide dismutase (SOD) were performed. Caspase-3 expression in nerve tissue was also investigated. The analyzed groups were compared with CG. Results. Biochemical investigation shows that there is a statistically significant difference between CG and only CIS and RG-50. Control group and RG-100 were found to be similar. Cisplatin-induced changes were observed in histopathological analysis of the nerve tissue. The RG-100 and CG were found to be similar. The caspase-3 expression in the neural tissue was compared between groups. Control group and CIS were found to be different. Control group and RG-100 were found to be similar. Conclusions. Antioxidant and antiapoptotic effectiveness of rutin was detected against the toxic effects caused by cisplatin in the peripheral nerve tissue.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2019Effects of pycnogenol on ischemia/reperfusion-induced inflammatory and oxidative brain injury in rats
Ozoner, Baris; Yuceli, Sahin; Aydin, Seckin; Yazici, Gulce Naz; Sunar, Mukadder; Arslan, Yusuf Kemal; Coban, Taha Abdulkadir; Suleyman, Halis
Background: Ischemia/reperfusion (I/R) injury results from the onset of re-circulation following a perfusion deterioration period in the tissues, resulting in more damage than that caused by perfusion deterioration. This study aimed to determine the effects of pycnogenol on I/R injury in rat brain tissues. Methods: Eighteen albino Wistar rats were divided into three groups: I/R injury (IR, n = 6) group; I/R injury + pycnogenol (IR + P, n = 6) group; and sham group (SG, n = 6). After 30 min of transient ischemia, 24 h of reperfusion was achieved in the IR and IR + P groups. Surgical dissection, except for transient ischemia, was performed in SG. Next, histopathological and biochemical investigations were performed on brain tissues. Malondialdehyde (MDA), reduced glutathione (GSH), and glutathione peroxidase (GPO) were analyzed as oxidative stress markers; IL-1β and TNF-α were analyzed as inflammatory stress markers in biochemical tests. Results: Histopathological examination revealed normal morphology in SG and diffuse cortex damage with edema, vasopathology, and inflammatory cell infiltration in the IR group. The IR + P group showed less cortex damage, edema, and vasopathology than the IR group. The MDA, IL-1β, and TNF-α levels were significantly higher in the IR group than those in the SG group. The values of same markers for the IR + P group were significantly lower than the IR group. The GSH and GPO levels were significantly decreased with IR damage, but PYC treatment showed significant improvement in the levels. Conclusion: This study showed that the administration of pycnogenol ameliorated brain damage after I/R injury by reducing oxidative and inflammatory damage in the rat brain.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2019Relationship between Serum Vitamin D levels and health-related quality of life in maintenance hemodialysis patients
Kara, Ali Veysel; Aldemir, Mehmet Naci; Soylu, Yasin Emrah; Arslan, Yusuf Kemal
Objectives: Aim of the study was investigating the effect of serum vitamin D levels on health-related quality of life in hemodialysis patients. Method: One-hundred and twenty-three maintenance hemodialysis patients were enrolled in this cross-sectional study. Patients divided into 2 groups according to serum vitamin D levels. A serum 25-hydroxyvitamin D (25[OH] D) level of < 20 ng/mL was identified as vitamin D deficiency (n = 78), and a serum level of ≥20 ng/mL was identified as normal (n = 45). Kidney Disease Quality of Life 36 (KDQOL-36) survey was used for quality of life measurement. Scores of the all of 5 subscales of KDQOL-36 were calculated. Multiple linear regression analyses were used to define independent risk factors affecting the survey. Results: Mean age of patients was 62 and 56% of patients were male. Mean 25(OH) D levels were 11.86 and 29.57 ng/mL, respectively, in 2 groups. There was statistically significant difference between age and Kt/V levels between 2 groups (p = 0.008 and p = 0.041). Age and gender were found as significant predictors of vitamin D deficiency (p = 0.026 and p = 0.021). In symptom and problem list subscale, gender and comorbidity were detected as independent risk factors (p = 0.050 and p = 0.032). Comorbidity was the only independent risk factor for effect of kidney disease subscale (p < 0.001). Independent risk factors associated with burden of kidney disease subscale were comorbidity and serum 25 (OH) D levels (p = 0.003 and p = 0.023). Serum 25(OH) D, gender, and comorbidity were independently associated with physical component summary (PCS) subscale (p < 0.001, p = 0.008, and p = 0.011). The only independently associated factor with mental component summary (MCS) was serum 25(OH) D (p < 0.001). Conclusion: We first showed the relationship between serum vitamin D levels and KDQOL-36 in hemodialysis patients. Lower serum vitamin D levels were negatively associated with burden of kidney disease, PCS, and MCS subscales.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2018Erzincan Bölgesindeki Çocukların D Vitamini Seviyelerinin Yaş, Cinsiyet ve Mevsimlere Göre Değerlendirilmesi
Arslan, Yusuf Kemal; Mertoglu, Cuma; Topal, İsmail; Sürücü Kara, İlknur; Peker, Necla; Gümüş, Alper
Amaç: D Vitamini, vücutta kalsiyum ve fosfor metabolizmasını düzenleyen, büyüme, gelişme ve sağlıklı bir iskelet yapısı için önemli bir hormondur. D vitamini eksikliği kemik dokusunun etkileri dışında birçok hastalıkların oluşmasında rolü olduğu bildirilmiştir. Bu çalışmada, Erzincan ilindeki çocuklarda D vitamini düzeylerinin retrospektif olarak aylara, cinsiyete, yaşa ve mevsimlere göre dağılımının değerlendirilmesi amaçlanmıştır. Gereç ve Yöntem: Çalışmamızda Ocak 2013–Aralık 2016 tarihleri arasında hastanemiz çocuk polikliniğine başvuran ve D vitamini ölçümü yapılan 2346 sonuç dâhil edildi. Hastalar Stage 6 kriterine bağlı olarak oluşturulan yaş gruplarına, cinsiyet ve 25 (OH ) D vitamini düzeyleri ay ve mevsimlere göre gruplara ayrıldı. Bulgular: Sonuçların %8,36’sı 10 ng/ml nin altında olup ciddi eksiklik grubunda,%42,33 20ng/ml altında olup hafif-orta derecede eksiklik ve %27,27 ng/ml olup yetersizlik grubunda yer almaktaydı. Erkeklerin kızlara göre daha yüksek D vitamini düzeyine sahip oldukları, yaş ilerledikçe D vitamini düzeylerinin azaldığı ve en yüksek D vitamini düzeyine yaz ayında en düşük D vitamini düzeyine ise kış ayında rastlanıldığı görülmüştür. Sonuç: İki yaş altı çocuklar hariç, tüm çocuklarda D vitamini düzeyi yetersizdir. Dolayısıyla bu yaş grubunda yeterli D vitamini düzeyi sağlamak için gerekli önlemler alınmalıdır. Hastalarda D vitamini düzeylerinin cinsiyete, yaşa ve mevsimlere bağlı olarak değişiklik gösterdiği sonucuna varılmıştır (p <0,001).
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2018Blastocystis spp.’nin Araştırılmasında Modifiye Formol-Eter Çöktürme Yöntemi, Ticari (Feconomics®) ve İki Farklı Modifikasyonunun Karşılaştırılması
Terzioğlu, Merve Aydın; Çolak, Meryem; Arslan, Yusuf Kemal; Al Bakkour, Katren; Doğruman Al, Funda; Oğuz, İlkiz; Kuştimur, Semra; Mumcuoglu, Ipek
Amaç: Çalışmada, ticari olarak geliştirilmiş bir dışkı yoğunlaştırmayöntemi olan Feconomics® (Salubris, Türkiye) ile Feconomics®’in ikifarklı modifikasyonunun, modifiye formol eter çöktürme (MFEÇ)yöntemiyle karşılaştırılarak Blastocystis spp.’nin mikroskobiktanısındaki etkinliğinin değerlendirilmesi amaçlanmıştır.Gereç ve Yöntem: Bu amaçla çeşitli gastrointestinal sistem yakınmalarıile gönderilen 750 hastaya ait dışkı örneği nativ-lugol yöntemi ileincelenmiş ve Blastocystis spp. saptanan 90 olgunun dışkı örneğiçalışmaya dâhil edilmiştir. Dışkı örnekleri rutin uygulanan MFEÇyöntemi, Feconomics® ve Feconomics®’in iki farklı modifikasyonu ileyoğunlaştırılmıştır. Örnekler yoğunlaştırmadan önce ve sonra nativlugol ve trikrom boyama yöntemi ile incelenmiştir. Nativ-lugol incelemeve trikrom boyama yöntemi, her alanda görülen protozoon sayısı veprotozoonların tipik morfolojik özellikleri açısından değerlendirilmiştir.Verilerin istatistiksel analizinde, IBM SPSS programı yardımıylaki-kare testi kullanılmıştır ve p<0.05 değeri anlamlı olarak kabuledilmiştir.Bulgular: MFEÇ yöntemi ile yapılan yoğunlaştırma sonrası nativlugol inceleme, direkt nativ-lugol inceleme ile karşılaştırıldığında,her alanda görülen protozoon sayısı açısından istatistiksel farkanlamlı bulunmamıştır (nativ: p=0.104; lugol: p=0.168). Feconomics®ve iki farklı modifikasyonu ile yapılan yoğunlaştırma sonrası nativlugol inceleme, direkt nativ-lugol inceleme ile karşılaştırıldığında iseher alanda görülen protozoon sayısı açısından istatistiksel farkanlamlı bulunmuştur (nativ: p=0.006, p=0.017, p=0.025; lugol:p<0.001, p<0.001, p=0.004). Feconomics® ve iki farklı modifikasyonusonrasında yapılan trikrom boyamada Blastocystis spp.’ninmorfolojisinin bozulduğu ve tipik görünümlerinin kaybolduğusaptanmıştır.Sonuç: Feconomics®’in nativ-lugol inceleme için parazitolojilaboratuvarlarında hızlı ve etkili bir yoğunlaştırma yöntemi olarakkullanılabileceği sonucuna varılmıştır.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2018Single level discectomy with and without disc prosthesis: A comparative study of 114 patients
Çakir, Tayfun; Çakir, Mürteza; Önder, Hilmiokay; Yolaş, Coşkun; Tanriverdi, Osman; Ömeroǧlu, Muhammed; Arslan, Yusuf Kemal
Background: Low back and leg pain due to lumbar discal hernia (LDH) is an important health issue. Current evidences support surgery in carefully selected patients who have failed conservative treatment and do not exhibit any psychosocial overlay. However, as known, sometimes it may be still very difficult to normalize the life qualities of patients for long times. Now different surgical methods for LDH are in use with new technological materials. One of them is lumbar disc prosthesis. In this study, the radiological and clinical effects of using lumbar disc prosthesis were evaluated with comparing patients underwent simple lumbar microdsicectomy. The purpose of this study is to reveal whether inserting the disc prosthesis into the intervertebral distance after lumbar microdiscectomy is beneficial or not both radiologically and clinically. Methods: A total of 114 patients were analyzed; the first cohort consisted of 57 patients who received a lumbar discectomy at a single level along with the implantation of a disc prosthesis and the second consisted of 57 patients only received a lumbar discectomy at a single level. These 2 groups were studied by comparing the disc space on the level of carried out operations with pain scales, foramen diameters of coming about related roots preoperatively and postoperatively at 3 years. Results: One of the significant results of the implementation of the disc prosthesis is fulfilment of a healthy disc height again after microdiscectomy due to LDH. We concluded that fulfilment of a healthy disc height with lumbar disc prosthesis was clinically beneficial for patients underwent microdiscectomy. Conclusions: Based on the results obtained in this study, it can be concluded that the implantation of a disc prosthesis in appropriate patients is more favorable regarding pain and spinal physiology when compared to simple microdiscectomy.
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