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- ArticleYalnız künye2021Effect of Taxifolin on Ischemia/Reperfusion-Induced Oxidative Injury of Sciatic Nerve in Rats
Yuceli, Sahin; Suleyman, Bahadir; Yazici, Gulce Naz; Mammadov, Renad; Cankaya, Murat; Kunak, Celaleddin Semih; Bulut, Seval; Suleyman, Halis; Altuner, Durdu
Background: Ischemia is a condition in which blood flow to tissues is decreased or entirely stopped for various reasons. The reperfusion process exacerbates damage caused by ischemia in the organs and tissues. Reactive oxygen species (ROS) are mainly responsible for ischemia-reperfusion (IR) damage. ROS increase results in lipid peroxidation (LPO) and oxidative stress. In the literature, taxifolin reportedly suppresses ROS production. This study aimed to determine the effect of taxifolin, which is a flavonoid, on IR injury of the sciatic nerve in rats. Methods: This study divided 30 albino Wistar rats into 3 groups: IR without medication (IR) group, taxifolin applied IR (TAX+IR) group, and only dissection made to the sciatic nerve sham group (SHAM). Sciatic nerve injury was induced by applying 2 hours of ischemia and 3 hours of reperfusion to the abdominal aorta and iliolumbar arteries. Biochemical and histopathologic investigations then were performed on sciatic nerve tissues. Malondialdehyde, total glutathione, glutathione reductase, and glutathione peroxidase were analyzed as oxidative stress markers, and tumor necrosis factor–α and interleukin-1β levels were evaluated as inflammatory stress markers in biochemical tests. Results: The IR group has statistically significantly high oxidant and cytokine levels and low antioxidant levels compared with the TAX+IR group. Taxifolin treatment was also shown to cause significant histopathologic improvement. Conclusions: We suggest that taxifolin may be effective in preventing IR injury of the sciatic nerve.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2020The effect of desloratadine on ischemia reperfusion induced oxidative and inflammatory renal injury in rats
Kocaturk, Huseyin; Bedir, Fevzi; Altay, Mehmet Sefa; Bakan, Ebubekir; Suleyman, Bahadir; Yazici, Gulce Naz; Sunar, Mukadder; Suleyman, Zeynep; Suleyman, Halis
Purpose: To examine the effect of desloratadine on kidney ischemia-reperfusion (I/R) injury in albino Wistar male rats using biochemical and histopathological methods. Methods: The treated with ischemia–reperfusion + 5 mg/kg desloratadine (IRD) group (n–6) was given 5 mg/kg desloratadine by gavage orally, and applied renal ischemia-reperfusion (BIR) group (n–6) and control (SG) group undergoing Sham operation (n–6) rats were given distilled water as solvent one hour before ketamine anesthesia. During the anesthesia period, ischemia was induced for 2 h unilaterally in the left kidney of all rats followed by reperfusion for 6 h. The kidneys of the SG group had sham operation without any intervention. Results: Our biochemical test results showed that malondialdehyde (MDA), nuclear factor kappa (NF-κB), tumor necrosis factor alpha (TNF-α), interleukin one beta (IL-1β), creatinine, and blood urea nitrogen (BUN) levels were significantly increased in the BIR group compared to the healthy control and IRD groups treated with desloratadine. Histopathological results were revealed tubular dilatation, tubular necrosis, loss of brushy margins, cast formation, and apoptotic bodies in tubular epithelial cells in the BIR group. There were no histopathological findings except for the swelling of tubule epithelial cells and the accumulation of proteinous material in some tubule lumens in renal tissue of desloratadine-treated rats. Conclusions: Experimental results suggested that desloratadine may be useful in the treatment of renal I/R injury.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2020The effect of Liv-52 on liver ischemia reperfusion damage in rats
Cimen, Orhan; Eken, Hüseyin; Keskin Cimen, Ferda; Cekic, Arif Burak; Kurt, Nezahat; Ozbek Bilgin, Asli; Suleyman, Bahadir; Suleyman, Halis; Mammadov, Renad; Pehlivanoglu, Kamil
Background: Liver ischemia reperfusion (I/R) damage which is frequently seen in clinical hepatobiliary surgeries has no effective treatment for it. Liv-52, known to have hepatoprotective effects, is a natural antioxidant drug licensed by the Ministry of Health of India. The aim of our study is to investigate the effect of Liv-52 on liver damage induced by I/R in rats. Methods: Albino Wistar male rats were divided into three groups; liver I/R (IR), 20 mg/kg Liv-52 + liver ischemia reperfusion (LIR) and sham operation applied to control group (HG). Liv-52 was administered to the LIR group (n = 6) 1 h prior to I/R application and distilled water was given orally to IR (n = 6) and HG (n = 6) groups as a solvent. Ischemia was determined as 1 h, and reperfusion was identified as 6 h in animals. Results: Increased levels of alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase, malondialdehyde, myeloperoxidase, and decreased levels of superoxide dismutase, and glutathione related enzymes caused by I/R application have been converged to healthy group level with Liv-52 treatment and the damage in liver tissue has been improved histopathologically. Conclusions: Liv-52 may be beneficial for preventing liver I/R damage in pre-surgery application.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2020The effect of cerebrolysin on cisplatin-induced oxidative acoustic nerve injury in rats
Erhan, Ertugrul; Bayram, Rana; Suleyman, Bahadir; Kurt, Nezahat; Suleyman, Halis; Salcan, Ismail
One of the major side effects of dose-limiting cisplatin is ototoxicity, resulting in restricted clinical use. Cerebrolysin is a neuroprotective, neurotrophic and antioxidant drug derived from the brains of pigs. No studies have investigated the positive effects of Cerebrolysin on the damage to auditory nerves caused by cisplatin- induced oxidative stress. Eighteen rats were randomly divided into three groups of six: the Cerebrolysin and cisplatin group (CCG), the cisplatin group (CG) and the healthy group (HG). The CCG was administered 2.5 mL/kg of Cerebrolysin via subcutaneous injection daily, while the CG and HG were administered physiological saline solutions daily. Every other day, after receiving their dose of Cerebrolysin or physiological saline solution, the CCG and CG groups were administered 5 mg/kg of cisplatin via intraperitoneal injection (IP) every other day. The substances were administered for eight days. In acoustic nerve tissue, while the CCís oxidant parameters were significantly higher (p < 0.0001) than those of the CCG and HG groups, its antioxidant parameters were also significantly lower (p < 0.0001). Cerebrolysin antagonized the balance of oxidants and antioxidants in acoustic nerve tissue, resulting in higher concentrations of oxidants. The results suggested that Cerebrolysin may be useful in the treatment of cisplatin-induced oxidative acoustic nerve injury.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2020Effect of rutin on cytarabine-associated pulmonary oedema and oxidative stress in rats
Bilgin, Asli O.; Mammadov, Renad; Suleyman, Bahadir; Unver, Edhem; Ozcicek, Fatih; Soyturk, Mehmet; Cimen, Ferda K.; Kurt, Nezahat; Suleyman, Halis
Cytarabine is effectively used in the treatment of adult acute leukemia, but it has a dose-limiting side effect of fatal pulmonary oedema because it increases the vascular permeability of the alveolar capillaries. The aim of the present study was to conduct a radiological, biochemical and histopathological investigation of the effect of rutin on cytarabine-associated pulmonary oedema in rats. Rats were treated with a combination of rutin+cytarabine by administering oral rutin at a dose of 50 mg/kg; other rat groups were orally administered the same volume of physiological saline. One hour after administration of rutin or saline, the rutin+cytarabine and cytarabine groups received an intraperitoneal injection of cytarabine (200 mg/kg). This administration procedure was repeated once a day for 14 days. Radiologically, 50% of the animals given cytarabine alone showed lung oedema, but the rutin+cytarabine group showed no oedema. The inclusion of rutin decreased the amounts of cytarabine-associated malondialdehyde, tumour necrosis factor-α, and nuclear factor-κB in the lung tissue. Rutin also inhibited the reduction of total glutathione by nitric oxide. These findings suggest that rutin may be a beneficial adjunct that can minimise the development of cytarabine-associated pulmonary oedema.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2019Effect of benidipine on experimental gastric ulcers in rats
Kuzucu, Mehmet; Suleyman, Bahadir; Mammadov, Renad; Ozcicek, Adalet; Ozcicek, Fatih; Altuner, Durdu; Süleyman, Zeynep
Peptic ulcer has been reported to increase acid secretion and lead to oxidative stress. Benidipine is an antihypertensive drug with antioxidant properties. This study aims to investigate the effects of benidipine on indomethacin-induced gastric ulcers in rats. Four groups were designed in this study. Gastric ulcer was occurred with indomethacin in the first three groups. These groups were treated with famotidine, benidipine and distilled water respectively 5 minutes before indomethacin administration. The fourth group rats didn’t have gastric ulcer and they received only distilled water. Six hours after drug administration gastric tissue was extracted and macroscopic and biochemical examinations were performed. Damaged areas in the stomach of benidipine and famotidine receiving animal groups were smaller than the indomethacin received animals. Indomethacin elevated the levels of malondialdehyde and myeloperoxidase in the stomach tissue (p<0.0001), and also decreased glutathione, glutathione related enzymes and superoxide dismutase (p<0.0001). Benidipine may be useful in preventing the toxic effect of indomethacin on the stomach.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2019Benidipine can prevent liver ischemia reperfusion injury in rats: a biochemical and histopathological evaluation
Cimen, Orhan; Eken, Hüseyin; Keskin Cimen, Ferda; Ozbek Bilgin, Asli; Pehlivanoglu, Kamil; Kurnaz, Eray; Gülaboglu, Mine; Suleyman, Bahadir; Suleyman, Halis
Ischemia reperfusion (IR) injury is defined as a pathological process which initiates with tissue deoxygenation, continues with oxidative stress, and spreads with inflammation. Increased intracellular calcium is responsible for oxidative stress and inflammation during this process. Benidipine is an antihypertensive drug acting as an T/L-type calcium channel blocker. The effect of benidipine on cyclooxygenase-2 (COX-2) activity is not known. Benidipine being a calcium channel blocker suggests that it may suppress oxidant and proinflammatory COX-2 production. The aim of this study was to examine the effect of benidipine on IR induced liver injury in albino Wistar male rats. Animals were divided into three groups: liver ischemia reperfusion (LIR), 2 mg/kg benidipine + liver ischemia reperfusion (BLIR), and sham surgery (SHAM). Hepatic artery, portal vein and bile duct were clamped to obtain ischemia for one hour and reperfusion for six hours. Malondialdehyde (MDA), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and COX-2 levels were higher, whereas total glutathione (tGSH) and cyclooxygenase-1 (COX-1) levels were lower in the LIR group. Liver tissue of the LIR group showed marked dilated congested blood vessels and increased bile duct proliferation, polymorphonuclear leukocyte infiltration, edema, hemorrhage and destruction. MDA, ALT, AST and COX-2 levels were lower, and tGSH and COX-1 levels were higher in the BLIR group treated with benidipine. Liver tissue of the BLIR group was found to be normal except for mild dilated blood vessels. These experimental findings indicate that benidipine may be useful in the treatment of liver IR injury.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2019Effect of ethyl acetate extract of usnea longissima o esophagogastric adenocarcinoma in rats
Mammadov, Renad; Suleyman, Bahadir; Altuner, Durdu; Demirci, Elif; Cetin, Nihal; Yilmaz, Adnan; Baykal, Huseyin; Alpcan, Hilal; Turumtay, Emine Akyuz; Suleyman, Halis
Purpose: To investigate the effects of the EtOAc extract of U. longissima which is uninvestigated previously on esophagogastric cancer induced in rats with N-methyl-N-nitro-N-nitrosoguanidin (MNNG). Methods: The anticancer activity of EtOAc extract of U. longissima was examined in the esophagogastric adenocarcinoma models induced in rats with MNNG. EtOAc extract of U. longissima, 50 and 100 mg/kg oral doses were administered once daily for six months. MNNG induced differentiated and undifferentiated type adenocarcinomas in the esophageal and gastric tissues of rats. Results: EtOAc extract of U. longissima obtained from U. longissima prevented gastric and esophageal cancerogenesis induced in rats with MNNG. EtOAc extract of U. longissima did not have a lethal effect at doses of 500, 1000 and 2000 mg/kg. The prominent anticarcinogenic activity of EtOAc extract of U. longissima 50 and 100 mg/kg suggests that it is not toxic and it is selective to the cancer tissue. Conclusion: This information may shed light on clinical implementation of EtOAc extract of U. longissima in future.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2019Effect of lutein on methotrexate-induced oxidative lung damage in rats: A biochemical and histopathological assessment
Mammadov, Renad; Suleyman, Bahadir; Akturan, Selcuk; Cimen, Ferda Keskin; Kurt, Nezahat; Suleyman, Zeynep; Malkoc, İsmail
Background/Aims: This study aimed to investigate the effect of lutein on methotrexate (MTX)-induced pulmonary toxicity in rats biochemically and histopathologically. Methods: The rats in the MTX + lutein (MTXL, n = 6) group were given 1 mg/kg of lutein orally. A 0.9% NaCl solution was administered orally to the MTX (n = 6) group and the healthy group (HG, n = 6). One hour later, a single 20 mg/kg dose of MTX was injected intraperitoneally in the MTXL and MTX. Lutein or 0.9% NaCl solution was administered once a day for 5 days. At the end of this period, malondialdehyde (MDA), myeloperoxidase (MPO), total glutathione (tGSH), interleukin 1 beta (IL-1β), and tumor necrosis factor alpha (TNF-α) were measured in the lung tissues from the animals euthanized with 50 mg/kg thiopental sodium anesthesia. Subsequently, histopathological examinations were performed. Results: The levels of MDA, MPO, IL-1β, and TNF-α in the lung tissue of the MTX were significantly higher than those of the MTXL and HG groups (p < 0.0001), and the amount of tGSH was lower. The histopathological findings in the MTX group, in which the oxidants and cytokines were higher, were more severe. Conclusions: Lutein prevented the MTX-induced oxidative lung damage biochemically and histopathologically. This result indicates that lutein may be useful in the treatment of MTX-induced lung damage.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2018The effect of esomeprazole on postoperative pain and lung and heart complications in rats
Onk, Didem; Coskun, Resit; Onk, Oruc A.; Cimen, Ferda K.; Kurt, Nezahat; Suleyman, Bahadir; Mammadov, Renad; Altuner, Durdu; Gul, Vahdet; Malkoc, Ismail
Postoperative pain begins with tissue incision, continues with the production of free oxygen radicals, and is intensified by the inflammatory response. We investigated the effect of esomeprazole on postoperative pain and lung and heart complications of laparotomy. Albino Wistar-type rats were grouped into the paw scalpel incision (PSI), applied control (SIC), PSI + 20 mg/kg esomeprazole (SIE), PSI + 20 mg/kg meperidine (SIM), and healthy group (HG). For the laparotomy test, the rats were grouped as follows: laparotomy applied control (LTC), laparotomy + 20 mg/kg esomeprazole (LTE), laparotomy + 20 mg/kg meperidine (LTM), and healthy group (HG). Meperidine suppressed the PSI-induced pain. Esomeprazole decreased the level of oxidant and proinflammatory cytokines in the serum and lung and heart tissues of the LTE group and in the paw tissues of the SIE group, but meperidine did not. There were no histopathological findings in the lung and heart tissues of the LTE group, but tissue damage was found in the LTC and LTM groups. Esomeprazole may help reduce major surgery complications.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2018The effect of thiamine and its metabolites on peripheral neuropathic pain induced by cisplatin in rats
Onk, Didem; Mammadov, Renad; Suleyman, Bahadir; Cimen, Ferda Keskin; Cankaya, Murat; Gul, Vahdet; Altuner, Durdu; Senol, Onur; Kadioglu, Yucel; Malkoc, Ismail
Thiamine pyrophosphate (TPP) is the active metabolite of thiamine. This study aimed to investigate the effects of thiamine and TPP on cisplatin-induced peripheral neuropathic pain (PNP). Male albino Wistar type Rattus norvegicus were divided into six groups (n=6) that received 2 mg/kg cisplatin (CIS), 25 mg/kg thiamine (TM), 2 mg/kg cisplatin+25 mg/kg thiamine (CTM), 25 mg/kg TPP (TPP), 2 mg/kg cisplatin+25 mg/kg TPP (CTPP), or distilled water (healthy group; HG) for 8 days intraperitoneally. Analgesic effect was measured with a Basile Algesimeter. IL-1β, malondialdehyde (MDA), total glutathione (tGSH), thiamine, and TPP were determined in blood samples. Histopathological examinations were performed on removed sciatic nerves. The percent analgesic effects of the CTM and CTPP groups were calculated to be 21.3% and 82.9%, respectively. Increased production of IL-1β and MDA by cisplatin was inhibited by TPP, while it was not inhibited by thiamine. Conversion of thiamine to TPP significantly decreased in the CIS group. Histopathological and biochemical investigations demonstrated that hyperalgesia and sciatic nerve damage developed in the CIS and CTM groups with low TPP levels. These results indicate that cisplatin inhibits the formation of TPP from thiamine, leading to severe PNP. This finding suggests that TPP may be more beneficial than thiamine for the treatment of cisplatin-induced PNP.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2018Effect of taxifolin on oxidative stress and amiodarone-induced pulmonary toxicity and fibrosis in rats
Suleyman, Bahadir; Bilgin, Asli O.; Mammadov, Renad; Cimen, Ferda K.; Gulaboglu, Mine
Amiodarone, an antiarrhythmic drug, is a benzofuran derivative. Oxidative stress plays a role in the mechanism of amiodarone-induced pulmonary toxicity. Taxifolin has antioxidant and anti-in-flammatory activities. The aim of the study was to investigate the effects of taxifolin on amiodarone-induced pulmonary toxicity in rats. In total 18 male albino Wistar rats were divided into three groups (n = 6 in each group): a taxifolin 50 mg/kg plus amiodarone 30 mg/kg (TAM) group, an amiodarone 30 mg/kg group (AMD) and a healthy group (HG). Malondialdehyde (MDA) levels, total glutathione (tGSH) levels, total oxidant status (TOS) and total antioxidant status (TAS) were measured in lung tissues, and histopathological examinations were performed. MDA and TOS levels in the AMD group were higher than those in the other two groups. In contrast, tGSH and TAS levels in the AMD group were lower than those in the TAM and HG groups. Alveolar destruction, marked oedema, alveolar septal fibrosis, leukocyte infiltration and haemorrhages were seen in the AMD group. Only slight oedema and congestion were observed in the TAM group. Taxifolin may be useful in preventing amiodarone-induced pulmonary toxicity.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2018Effect of anakinra, an interleukin one beta antagonist, on oxidative testicular damage induced in rats with ischemia reperfusion; [Efecto de la anakinra, que es un antagonista de la interleucina 1 beta en oxidativo daño testicular, inducida en ratas con isquemia reperfusión]
Hirik, Erkan; Suleyman, Bahadir; Mammadov, Renad; Yapanoglu, Turgut; Cimen, Ferda Keskin; Cetin, Nihal; Kurt, Nezahat
Background: It has been reported in the literature that proinflammatory interleukin-1 beta (IL-1β) is increased in cases of testicular ischemia reperfusion (I/R) damage. This information suggests that anakinra, an IL-1β antagonist, may be effective in testicular I/R damage. Objective: In our study, we investigated the effect of anakinra on testicular I/R damage induced in rats with torsion/detorsion. Methods: The 50 mg/kg anakinra + testicular torsion/detorsion (KTD-50) and 100 mg/kg anakinra + testicular torsion/detorsion (KTD-100) groups received an intraperitoneal (i.p.) injection of 50 mg/kg and 100 mg/kg of anakinra, respectively. In turn, the testicular torsion/detorsion (TTD) and sham operation (SOG) groups received a single dose of distilled water as a solvent 1 h before ketamine anaesthesia. After the testes of the TTD, KTD-50 and KTD-100 groups were subjected to torsion and detorsion for 4 h each, the rats were killed with a high-dose anaesthesia, and their testicles were removed and evaluated through biochemical, gene expression and histopathological examinations. The results were evaluated in comparison with those of the SOG group. Results: The levels of malondialdehyde (MDA), myeloperoxidase (MPO) and IL-1β showed significant increases in the TTD group, which underwent torsion/detorsion, compared to the KTD-50, KTD-100 and SOG groups. Conversely, the levels of glutathione (tGSH), glutathione peroxidase (GPO) and glutathione s-transferase (GST) were found to be significantly higher in the KTD-50, KTD-100 and SOG groups than in the TTD group. Conclusion: Anakinra at a 100 mg/kg dose histologically suppressed better oxidative stress and tunica albuginea, germ cell, seminiferous tubule and interstitial damage in the testicular tissue compared to a 50 mg/kg dose. Experimental results indicate that anakinra might be beneficial in the attenuation of testicular I/R damage. © 2017 Asociación Española de Andrología, Medicina Sexual y Reproductiva; Antecedentes: Se ha reportado en la literatura que citoquinas interleuquina-1 beta (IL-1β) es mayor en el daño de la isquemia reperfusión testicular (I/R). Esta información sugiere que la anakinra, que es un antagonista IL-1β puede ser eficaz en daño testicular I/R. Objetivo: En nuestro estudio se investigó el efecto de este medicamento en daño testicular I/R inducida en ratas con detorsion/torsión. Métodos: KTD-50 grupo recibido intraperitonealmente (i.p.) inyección de 50 mg/kg y KTD-100 Grupo 100 mg/kg de anakinra, mientras TTD (control) y SOG (sham grupo operación) recibieron una dosis única de agua destilada como solvente, una hora antes de ketamina anestesia. Después de que los testículos de TTD, KTD-50 y KTD-100 grupos fueron sometidas a torsión y detorsion para cuatro por cuatro horas, las ratas fueron asesinados con altas dosis de anestesia, sus testículos fueron extraídos y evaluados a través de la expresión génica, bioquímicas e histopatológicas de exámenes. Los resultados fueron evaluado en comparación con la de SCG grupo. Resultados: Los niveles de MDA, MPO y IL- 1β mostraron incrementos significativos en el grupo TTD/torsión detorsion administrados frente a-50, KTD KTD-100 y SOG grupos. Por el contrario, los niveles de tGSH, GPO y GST resultaron significativamente más altas en KTD-50 KTD-100 y grupos SOG de TTD en grupo. Conclusión: La anakinra en 100 mg/kg dosis mejor histológicamente suprime el estrés oxidativo y la túnica albuginea, células germinales, túbulos seminíferos apretadamente enrollados intersticial y daño en el tejido testicular en comparación con la dosis de 50 mg/kg. Los resultados experimentales indican que la anakinra puede ser beneficiosa en la atenuación de los daños I/R testicular.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2018The protective effect of thiamine pryophosphate against sugar-induced retinal neovascularisation in rats
Cinici, Emine; Mammadov, Renad; Findik, Huseyin; Suleyman, Bahadir; Cetin, Nihal; Calik, Ilknur; Balta, Hilal; Tas, Ismail Hakki; Sener, Ebru; Altuner, Durdu
The aim of this study was to investigate the effect of thiamine pyrophosphate (TPP), administered via sugar water, on retinal neovascularisation in rats. Animals were assigned to three groups, namely the TPP sugar-water group (TPSWG, n = 12), the control group (CG, n = 12) and the healthy group (HG, n = 12). The TPSWG was injected intraperitoneally with TPP once a day for 6 months. CG and HG rats were given distilled water in the same way. TPSWG and CG rats were left free to access an additional 0.292 mmol /ml of sugar water for 6 months. The fasting blood glucose (FBG) levels of the animals were measured monthly. After 6 months, biochemical, gene expression and histopathologic analyses were carried out in the retinal tissues removed from the animals after they were killed. The measured FBG levels were 6.96 ± 0.09 mmol/ml (p < 0.0001 vs. HG), 6.95 ± 0.06 mmol/ml (p < 0.0001 vs. HG) and 3.94 ± 0.10 mmol/ml in the CG, TPSWG and HG groups, respectively. The malondialdehyde (MDA) levels were found to be 2.82 ± 0.23 (p < 0.0001 vs. HG), 1.40 ± 0.32 (p < 0.0001 vs. HG) and 1.66 ± 0.17 in the CG, TPSWG and HG, respectively. Interleukin 1 beta (IL-1β) gene expression was increased (3.78 ± 0.29, p < 0.0001) and total glutathione (tGSH) was decreased (1.32 ± 0.25, p < 0.0001) in the retinal tissue of CG compared with TPSWG (1.92 ± 0.29 and 3.18 ± 0.46, respectively). Increased vascularisation and oedema were observed in the retinal tissue of CG, while the retinal tissues of TPSWG and HG rats had a normal histopathological appearance. A carbohydrate-rich diet may lead to pathological changes in the retina even in nondiabetics, but this may be overcome by TPP administration.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2018The effect of parecoxib on testicular ischemia/reperfusion injury in rats
Bayrak, Oguzhan; Cimen, Ferda K.; Hirik, Erkan; Gulaboglu, Mine; Suleyman, Zeynep; Suleyman, Bahadir
The aim of this study was to investigate the effects of parecoxib on the ischemia/reperfusion (I/R) injury induced in rats by torsion-detorsion. Parecoxib was injected to all the rats intraperitoneally once prior to the torsion-detorsion. The ischemia/reperfusion and sham surgery groups were given the same volume of distilled water. One h later, torsion and detorsion were performed, each for two h, on the ischemia/reperfusion + 10 mg/kg parecoxib, ischemia/reperfusion + 20 mg/kg parecoxib, and ischemia/reperfusion groups. The results of the biochemical test showed that 20 mg/kg dose of parecoxib was more effective than the 10 mg/kg dose in inhibiting the increase in the MDA, COX-2, IL-1β and TNF-α levels and decrease in tGSH and COX-1 in the testicular tissue treated with torsion-detorsion. Furthermore, it was also histopathologically shown that the 20 mg/kg dose better prevented torsion-detorsion injury. The experimental results suggest that parecoxib will be clinically useful in the treatment of testicular I/R injury due to torsion-detorsion.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2018The effects of ketamine and thiopental used alone or in combination on the brain, heart, and bronchial tissues of rats
Ahiskalioglu, Elif Oral; Aydin, Pelin; Ahiskalioglu, Ali; Suleyman, Bahadir; Kuyrukluyildiz, Ufuk; Kurt, Nezahat; Altuner, Durdu; Coskun, Resit; Suleyman, Halis
Introduction: We compared the side effects of ketamine and thiopental used alone and of a ketamine/thiopental combination dose on the brain,heart, and bronchial tissues of rats. Material and methods: Three groups received intraperitoneal injections of 30 mg/kg ketamine (K-30); 15 mg/kg thiopental (T-15); or of both in combination (KTSA). These doses were doubled in another set of study groups (K-60, T-30, and KTA groups, respectively). Optimal anesthesia duration was examined in all groups. Results: Anesthesia did not occur with 30 mg/kg ketamine or 15 mg/kg thiopental. However, when used alone ketamine and thiopental led to oxidative stress in the striatum, heart, and bronchial tissues. Conversely, combined administration of anesthetics and subanesthetic doses were found not to create oxidative stress in any of these areas. The highest level of adrenaline in blood samples collected from the tail veins was measured in the KTA-60, and the lowest amount in the T-30. Creatine kinase activity was highest in the KTA-60 group (p < 0.001). When we compared for all 5 groups to untreated control group; the creatine kinase-MB activities were significiantly different in K-30, T-15 and T-30 (p < 0.001). Conclusions: The studied doses of ketamine led to oxidative stress by increasing the amount of adrenaline. Thiopental increased oxidative stress with decreases in adrenaline. A longer anesthetic effect with minimal adverse events may be achieved by ketamine and thiopental in combination.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2017Effect of sheep tail fat on the knee joint cartilage injury induced in rats with formalin
Cimen, Ferda Keskin; Kockara, Nizamettin; Turkoglu, Murat; Dundar, Cihat; Cetin, Nihal; Suleyman, Bahadir; Coban, Abdulkadir; Yarali, Oguzhan; Malkoc, Ismail; Suleyman, Halis
Background: The effect of sheep tail fat (STF) on the damage induced in the knee articular joint with formalin was investigated and evaluated from the biochemical, gene expression, histopathological aspects in comparison with an extract consisting of glucosamine hydrochloride, chondroitin sulfate, methylsulfonylmethane, Harpagophytum procumbens and bromelain (GCMHB). Material and methods: The animals were divided into four groups: The first group received formalin injection to the knee joint as FCG control. The second group received 500 mg/kg STF + formalin (STFF). The third group was given 500 mg/kg GCMHB + formalin (GCMHBF). The fourth group was the healthy group (HG). The STF and GCMHBF were administered at 500 mg/kg dose. After 1 h of the STF and GCMHB administration 0.2 ml 1% formalin was injected into the knee joints of all rats except the HG group. The STF and GCMHB were given once a day at the specified doses over 30 days. Results: It was observed that, STF and GCMHB prevented the increase of IL-1β, TNF-α and COX-2 gene expressions in the cartilage tissue after formalin damage. In addition, STF and GCMHB improved the histopathological disturbances in the cartilage tissue caused by formalin. Conclusion: Since it is an inexpensive and easily available natural product, Sheep Tail Fat could be used in clinical practice along with the other medicines to prevent cartilage damage.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2017Effect of Hippophae rhamnoides extract on oxidative oropharyngeal mucosal damage induced in rats using methotrexate
Erhan, Ertugrul; Terzi, Suat; Celiker, Metin; Yarali, Oguzhan; Cankaya, Murat; Cimen, Ferda Keskin; Malkoc, Ismail; Suleyman, Bahadir
Objectives. The objective of this study is to investigate and evaluate the effect of Hippophae rhamnoides extract (HRE) on oropharyngeal mucositis induced in rats with methotrexate (MTX) through biochemical, gene expression, and histopathological examinations. Methods. Experimental animals were divided into a healthy group (HG), a HRE+MTX (HREM) group, HRE group (HREG), and a control group that received MTX (MTXG). The HREM and HREG groups of rats was administered 50 mg/kg HRE, while the MTXG and HG groups were given an equal volume distilled water with gavage. Then, the HREM and MTXG rat groups were given oral MTX at a dose of 5 mg/kg 1 hour after HRE and distilled water was administered. This procedure was repeated for 1 month. At the end of this period, all of the animals were sacrificed with a high dose of anesthesia. Then, the amounts of malondialdehyde (MDA) and total glutathione (tGSH) were determined in the removed oropharyngeal tissues. Interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) gene expressions were measured, and all the tissues were studied histopathologically. Results. The amount of MDA was significantly increased in the MTXG group compared to the HREM, HREG, and HG groups (P<0.001). MTX significantly decreased the amount of tGSH in the MTXG group compared to the HREM, HREG, and HG groups (P<0.001). In this study, there were no visible ulcers in the animal group in which the levels of MDA, IL-1β, and TNF-α were high and the level of tGSH was low. However, histopathologic examination revealed mucin pools in wide areas due to ruptured oropharynx glands, and proliferated, dilated, and congested blood vessels and dilated ductal structures in some areas. Conclusion. HRE protected oropharyngeal oxidative damage induced by MTX. As an inexpensive and natural product, HRE has important advantages in the prevention of oropharyngeal damage induced by MTX.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2016The Impact of Resveratrol on Oxidative Stress Induced by Methotrexate in Rat Ileum Tissue: Evaluation of Biochemical and Histopathological Features and Analysis of Gene Expression
Ozcicek, Adalet; Cetin, Nihal; Keskin Cimen, Ferda; Tumkaya, Levent; Malkoc, Ismail; Gulaboglu, Mine; Yarali, Oguzhan; Suleyman, Bahadir
Objective: The aim of this study was to assess the impact of resveratrol (RST) on oxidative stress induced by methotrexate in rat ileum tissue. Materials and Methods: Twenty-four rats were divided into 4 groups with 6 in each group. Each rat was orally administered the following every day for 30 days: group 1 (MTXG), methotrexate (MTX; 5 mg/kg); group 2 (RMTXG), MTX (5 mg/kg) plus RST (25 mg/kg/day); group 3 (RSTG), RST alone (25 mg/kg/day), and group 4 (controls), distilled water. After the rats had been sacrified, the ilea were removed for the assessment of malondialdehyde (MDA), total glutathione (tGSH) and glutathione peroxidase (GSH-Px). Gene expression analyses for interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and myeloperoxidase (MPO) were also performed. Hematoxylin and eosin-stained paraffin-embedded sections of the ileum were analyzed under a light microscope and the findings were recorded. Statistical analyses of the data were performed using one-way ANOVA. Results: The administration of MTX in group 1 yielded a higher level of MDA (8.33 ± 2.5 μmol/g protein, p < 0.001) and lower levels of tGSH (0.97 ± 0.29 nmol/g protein) and GSH-Px (5.22 ± 0.35 U/g protein, p < 0.001) compared to the other groups. MTX also increased IL-1β (40.33 ± 5.43 gene expression levels), TNF-α (6.08 ± 0.59) and MPO gene expression (9 ± 1.41) in group 1 compared to the controls (11.33 ± 2.07, 2.15 ± 0.33 and 3.43 ± 0.48, respectively, p < 0.001). The impact of RST on IL-1β, TNF-α and MPO gene expression induced by MTX was observed as a reversal of these findings (p < 0.05). Severe inflammation, damage to the villus epithelium and crypt necrosis was observed histopathologically in the MTXG group, whereas only mild inflammation was seen in the RMTXG group. Conclusion: In this study, ileal damage caused by MTX was inhibited by RST.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2016Effects of nimesulide on oxidative mucosal injury induced by cisplatin in rat duodenum and jejunum
Ozcicek, Fatih; Cankaya, Murat; Cimen, Ferda K.; Kafa, Ayse H.T.; Nalkiran, Hatice S.; Suleyman, Bahadir; Altuner, Durdu; Cetin, Nihal
In this study, the impact of nimesulide on oxidative stress induced by cisplatin in rat duodenum and jejunum tissue was assessed by evaluating the biochemical and histopathological changes. The rats in the study were assigned to three groups: the control group and cisplatin (Cis) group were given distilled water, and the nimesulide + cisplatin (Nim + Cis) group was given nimesulide (50 mg/kg) orally for seven days. The Cis and Nim + Cis groups were injected with a single dose of intraperitoneal cisplatin (6 mg/kg) on the first day. After the rats were euthanized, the duodenum and jejunum of each rat was removed for the assessment of biochemical markers and histopathological evaluation. Cisplatin administration in the Cis group resulted in increased levels of malondialdehyde and nitric oxide and decreased levels of total glutathione, glutathione reductase, and catalase compared to the healthy and Nim + Cis groups. Nimesulide significantly inhibited the increase of oxidants (p < 0.001) and the decrease of antioxidants caused by cisplatin (p < 0.001). Inflammation, damage to the villus epithelium, hemorrhage, and capillary proliferation were determined histopathologically in the Cis group. The results of this study indicate that oxidative stress caused by cisplatin may be preventable by co-administered nimesulide.
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