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- ArticleAçık Erişim2024Lacidipine, thiamine pyrophosphate and their combination on the ocular ischemic syndrome induced by bilateral common carotid artery ligation
Cicek, Ibrahim; Somuncu, Ahmet Mehmet; Altuner, Durdu; Suleyman, Bahadir; Mammadov, Renad; Bulut, Seval; Coban, Taha Abdulkadir; Tastan, Tugba Bal; Suleyman, Halis
AIM: To investigate the effect of lacidipine, thiamine pyrophosphate (TPP) and the combination of lacidipine and TPP against oxidative and inflammatory eye damage induced by bilateral common carotid artery ligation in rats.. METHODS: Male albino Wistar rats were categorized as those who underwent sham surgery (SG), right and left common carotid cross-clamping and unclamping procedure (CCU), lacidipine+CCU (LCCU), TPP+CCU (TCCU), and combination of lacidipine and TPP (LTC)+CCU (LTCCU). One hour before anesthesia, the LCCU (n=6) received lacidipine (4 mg/kg, orally) and the TCCU (n=6) received TPP (20 mg/kg, intraperitoneally). The SG (n=6) and CCU (n=6) received the same volume of distilled water from the same route. After anesthesia (60 mg/kg ketamine, intraperitoneally), the necks of the rats were opened in the midline. Ischemia was created for 10min by placing clips on the right and left common carotid arteries. Rats in the SG only underwent subcutaneous incision. After 10min, the clips were removed and reperfusion was achieved for six days. Then, the animals were euthanized (120 mg/kg ketamine, intraperitoneally) and the levels of oxidant, antioxidant and proinflammatory cytokines in the eye tissues were determined. The retinal tissue of the eye was also examined histopathologically.. RESULTS: Lacidipine, TPP, and LTC significantly prevent the increase in malondialdehyde, tumor necrosis factor-alpha, interleukin-1β (IL-1β), and IL-6 levels, decrease in total glutathione levels, superoxide dismutase and catalase activities and histopathological retinal damage in eye tissue induced by bilateral common carotid artery ligation in rats. The impact of these drugs on protection is determined to be LTC>lacidipine>TPP.. CONCLUSION: As a result of the study, it is concluded that LTC may be more effective than lacidipine and TPP alone in treating ocular ischemic syndrome.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Adverse effects of metamizole on heart, lung, liver, kidney, and stomach in rats
Ciftel, Sedat; Suleyman, Bahadir; Mammadov, Renad; Coskun, Resit; Coban, Taha A.; Mokhtare, Behzad; Suleyman, Halis; Cerrah, Serkan; Cicek, Betul; Suleyman, Zeynep
Background: Metamizole is banned in some countries because of its toxicity, although it is widely used in some European countries. In addition, there is limited information on its safety profile, and it is still debated whether it is toxic to the heart, lungs, liver, kidneys, and stomach. Aims: Our study investigated the effects of metamizole on the heart, lung, liver, kidney, and stomach tissues of rats. Methods: Eighteen rats were divided into three groups, wassix healthy (HG), 500 mg/kg metamizole (MT-500), and 1000 mg/kg metamizole (MT-1000). Metamizole was administered orally twice daily for 14 days. Meanwhile, the HG group received pure water orally. Biochemical, histopathologic, and macroscopic examinations were performed on blood samples and tissues. Results: Malondialdehyde (MDA), total glutathione (tGSH), superoxide dismutase (SOD), and catalase (CAT) in the lung and gastric tissues of MT-500 and MT-1000 groups were almost the same as those of the HG (p > 0.05). However, MDA levels in the heart and liver tissues of MT-500 and MT-1000 groups were higher (p < 0.05) compared to the HG, while tGSH levels and SOD, and CAT activities were lower (p < 0.05). MDA levels of MT-500 and MT-1000 groups in the kidney tissue increased the most (p < 0.001), and tGSH levels and SOD and CAT activities decreased the most (p < 0.001) compared to HG. Metamizole did not cause oxidative damage in the lung and gastric tissue. While metamizole did not change troponin levels, it significantly increased alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), and creatinine levels compared to HG. Histopathologically, mild damage was detected in heart tissue, moderate damage in liver tissue, and severe damage in renal tissue. However, no histopathologic damage was found in any groups’ lung and gastric tissues. Conclusion: Metamizole should be used under strict control in patients with cardiac and liver diseases and it would be more appropriate not to use it in patients with renal disease.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023Effect of Liv-52 on Atorvastatin Induced Hepatotoxicity in Rats: A Biochemical and Histopathologic Study
Icel, Aykut; Suleyman, Bahadir; Mammadov, Renad; Bulut, Seval; Cicek, Betul; Yazici, Gulce Naz; Gulaboglu, Mine; Ozcicek, Fatih; Suleyman, Halis
Background and Objective: The toxic effect of atorvastatin on the liver has been associated with oxidative stress. The Liv-52 is a polyherbal formulation with known hepatoprotective properties. In this study, the preventive effect of Liv-52 against the potential hepatotoxicity of atorvastatin was investigated. Materials and Methods: Eighteen rats were categorized into three groups of six rats each: Healthy group (HG), atorvastatin-treated group (ATC) and Liv-52+atorvastatin-treated group (LAT). The Liv-52 was orally administered at 50 mg kg(-1) and atorvastatin was orally given at 20 mg kg(-1) after 1 hr of Liv-52 administration. The Liv-52 and atorvastatin were administered once daily for two months. At the end of two months, blood samples were collected from the rats. Subsequently, rats were sacrificed under high-dose (50 mg kg(-1)) thiopental anesthesia and liver tissues were extracted. Biochemical analysis of extracted liver tissues and serum was performed. Liver tissues were additionally analyzed for histopathology. Results: The Liv-52+atorvastatin administration inhibited the atorvastatin-induced increase in malondialdehyde and decreased total glutathione and superoxide dismutase activities in the liver tissues. In addition, the rats receiving Liv-52 had lower levels of serum alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase compared to the atorvastatin group. Histopathologic analysis demonstrated that Liv-52 protected the liver tissue against atorvastatin-induced injury. Conclusion: The Liv-52 therapy may be useful in preventing atorvastatin-induced liver injury.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2023Effect of Taxifolin on Lung Injury Induced by Staphylococcus aureus in Albino Wistar-Type Rats: A Biochemical and Histopathological Evaluation
Dabanlioglu, Bulent; Suleyman, Bahadir; Mammadov, Renad; Yavuzer, Bulent; Altuner, Durdu; Akkas, Onder; Mokhtare, Behzad; Coban, Taha Abdulkadir; Suleyman, Halis
Background and Objective: Staphylococcus aureus is considered the main cause of pneumonia. Pneumonia is the most common cause of acute lung injury. Taxifolin is a flavonol with antioxidant, anti-inflammatory, antimicrobial, antiviral, antifungal, anti-hyperglycemic, anti-hyperlipidemic, anti-psoriatic and pulmono-protective activities. The present study aimed to biochemically and histopathologically investigate the protective effect of taxifolin against possible acute lung oxidative and inflammatory damage caused by Staphylococcus aureus ( S. aureus) infection in rats. Materials and Methods: The rats were divided into three groups 6 rats each, healthy control (HG), S. aureus inoculated (SaG) and S. aureus inoculated+taxifolin treated (SaT). Animals were euthanized. Malondialdehyde (MDA) total glutathione (tGSH), Nuclear Factor kappa B (NF-kappa B), Tumor Necrosis Factor-alpha (TNF-alpha) and Interleukin One Beta (IL-1 beta) levels were measured in the excised lung tissues and also examined histopathologically. Results: S. aureus inoculation significantly increased MDA, NF-kappa B, TNF-alpha and IL-1 beta levels and decreased tGSH levels in lung tissue. Treatment with taxifolin significantly suppressed the increase in MDA, NF-kappa B, TNF-alpha and IL-1 beta levels and the decrease in tGSH levels. Histopathologically, S. aureus inoculation led to severe mononuclear cell infiltration in interstitial areas, lymphoid hyperplasia in bronchial-associated lymphoid tissue and desquamation in bronchial epithelium. Treatment with taxifolin attenuated these histopathological findings. Conclusion: Current experimental results suggested that taxifolin may be beneficial in the treatment of oxidative and inflammatory lung injury associated with S. aureus infection.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2023Effect of Cinnamon Extract on Ovarian Torsion Detorsion Damage in Rats
Bingul, Eda; Hizal, Elif; Suleyman, Bahadir; Mammadov, Renad; Bulut, Seval; Altuner, Durdu; Tastan, Tugba Bal; Dogru, Tugba Erkmen; Suleyman, Halis
Background and Objective: The torsion and subsequent detortion of the ovaries causes oxidative and inflammatory damage. Cinnamon is an herbal agent with many activities such as antioxidant and anti-inflammatory. This study investigates biochemically and histopathologically the protective effect of cinnamon extract (CE) in potential ischemia-reperfusion (I/R) injury induced by torsion detorsion of ovarian tissue in rats. Materials and Methods: The rats were divided into healthy (HG), sham operation (SOG), ovarian I/R (OIR) and CE (100 mg kgG1 CE, oral, 6 days)+ovarian I/R (COIR) groups. For I/R, right ovaries were subjected to ischaemia for 3 hrs followed by reperfusion for 6 days. Ovarian tissues were analysed for oxidants, antioxidants and proinflammatory cytokines. The histological structure of the excised tissues was also examined. Results: Oxidants (malondialdehyde) and proinflammatory cytokines (nuclear factor kappa B, tumor necrosis factor-alpha, interleukin 1-beta, interleukin-6) increased and antioxidants (total glutathione, superoxide dismutase, catalase) decreased in ovarian tissues of OIR compared to HG (p<0.001). Oxidant and proinflammatory cytokines were lower and antioxidants were higher in rats receiving CE treatment with the I/R procedure compared to OIR (p<0.001). Histological damage observed in the OIR group was found to be attenuated in the COIR group. Conclusion: The CE may protect the ovarian tissue from I/R damage with its antioxidant and anti-inflammatory activity.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2023Effect of Thiamine Pyrophosphate Upon Oxidative Brain Injury Induced by Ischemia-Reperfusion in Rats
Yasar, Hasan; Demirdogen, Filiz; Suleyman, Bahadir; Mammadov, Renad; Altuner, Durdu; Coban, Taha Abdulkadir; Bulut, Seval; Gursul, Cebrail; Suleyman, Halis
Background and Objective: Exposure of the brain to Ischemia-Reperfusion (I/R) may cause tissue damage through oxidative stress. Thiamine pyrophosphate (TPP), which has a protective effect against oxidative stress, is the active metabolite of vitamin B-1. In this study, the protective effect of TPP against possible I/R damage of brain tissue was investigated. Materials and Methods: Thirty rats were randomly divided into BIR, TIR and HG groups consisting of ten rats. In the TIR group, 20 mg kgG1 TPP was injected intraperitoneally (ip). After 1 hr, clips were placed in the common carotid arteries of the BIR and TIR groups under anesthesia. Brain tissue was subjected to ischemia for 10 min. Afterward, the clips were opened and 3 hrs of reperfusion was achieved. In the HG group, only subcutaneous incisions were made and closed. Then, the brain tissues removed by euthanasia were biochemically analyzed. Results: While, I/R increased malondialdehyde (MDA), myeloperoxidase (MPO), Tumor Necrosis Factor-alpha (TNF-alpha), Interleukin-1 beta (IL-beta) and 8-Hydroxyguanine (8-OHGua) levels in brain tissues, total caused a decrease in glutathione (tGSH), glutathione peroxidase (GPO) and glutathione reductase (GSHRd) levels (p<0.001). The TPP applied before I/R significantly prevented these changes (p<0.05). Conclusion: The results of biochemical tests suggested that TPP may be beneficial in preventing possible brain damage due to I/R.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023Effect of Lercanidipine, Carvacrol and Their Combination on Ischemia and Reperfusion-induced Distant Organ (Heart) Damage in Rats
Emir, Izzet; Altuner, Durdu; Suleyman, Bahadir; Mammadov, Renad; Suleyman, Zeynep; Coskun, Resit; Coban, Taha Abdulkadir; Suleyman, Halis
Background and Objective: Ischemia/reperfusion (I/R) damage has still been a reason for high morbidity and mortality for patients. Systemic damage is also observed in addition to local damage in lower extremity I/R event. It was purposed to research the protective effect of lercanidipine, carvacrol and lercanidipine, carvacrol their combination (LCC) against cardiac damage possible to develop after the I/R procedure administered to the lower extremities in rats. Materials and Methods: All rats were assigned into five groups, as sham operation (SG), only performed ischemia/reperfusion (I/R) to the lower extremity (IRG), lercanidipine+I/R (LIR), carvacrol+I/R (CIR) and lercanidipine, carvacrol combination+I/R (LCIR), randomly. One hour prior to anesthesia, 10 mg kg(-1) of lercanidipine was given to the LIR group. Carvacrol (50 mg kg(-1)) was injected to the CIR group. Lercanidipine (10 mg kg(-1)) was administered and carvacrol (50 mg kg(-1)) to the LCIR group. In IRG, LIR, CIR and LCIR groups, the ischemia was induced-occluding the femoral-artery for 4 hrs, reperfusion was for 24 hrs. Results: It was observed that malondialdehyde (MDA), troponin I (TPI), Creatine Kinase (CK-MB) and Interleukin 6 (IL-6) levels were lower in the heart tissue applied to LCC and total glutathione, superoxide dismutase and catalase levels were higher than the ones in the groups treated with lercanidipine and carvacrol. Conclusion: Current study suggested that lercanidipine and carvacrol were efficient in the treatment of heart injury, but LCC provided a more efficient treatment. These findings indicated that LCC alone was possible to be better than lercanidipine and carvacrol in reducing the severity of I/R-induced heart damage that occurred in any part of the body.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023Comparative Evaluation of Ovarian Ischemia-Reperfusion Induced Distant Organ Damage in Rats
Bicer, Senol; Suleyman, Bahadir; Mammadov, Renad; Yavuzer, Bulent; Altuner, Durdu; Gursul, Cebrail; Coban, Taha Abdulkadir; Coskun, Resit; Suleyman, Halis
Background and Objective: Ischemia-reperfusion (I/R) injury is seen in many conditions, including ovarian torsion/detorsion. Ovarian ischemia is an emergency that occurs as a result of torsion of the ovaries. This study aimed to investigate the extent to which brain, heart, lung, kidney and liver tissues were affected by ovarian ischemia-reperfusion damage in albino Wistar-type female rats and to evaluate them by comparison. Materials and Methods: Animals were divided into two groups: The group to be applied sham operation (SG) and the group to be applied the ischemia-reperfusion procedure to the ovaries (OIR). As 2 hrs of ischemia and 6 hrs of reperfusion were conducted in the right ovaries of OIR group rats. After reperfusion, the ovarian, brain, heart, lung, kidney and liver tissues of the animals sacrificed were removed. The severity of tissue damage was assessed by the degree of significance of the increase in oxidative and proinflammatory parameters and the decrease in antioxidants. Results: The organs obtained from the OIR group in which MDA and IL-6 levels were highest, while tGSH, SOD and CAT levels were the lowest, were ovary>kidney>lung>heart, respectively. The levels of these parameters in the brain and liver tissues were found to be almost the same as in the SG group. Current findings showed that ovarian ischemia-reperfusion damage did not affect brain and liver tissues, but the heart was mildly affected, the lung was moderately and kidney tissue was severely affected. Conclusion: Current experimental results showed that it may be useful to consider the possibility of damage to organs such as the heart, lungs and kidneys before and after the ovarian ischemia-reperfusion procedure and take the necessary precautions.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023Carvacrol prevents acrylamide-induced oxidative and inflammatory liver damage and dysfunction in rats
Cerrah, Serkan; Ozcicek, Fatih; Gundogdu, Betul; Cicek, Betul; Coban, Taha Abdulkadir; Suleyman, Bahadir; Altuner, Durdu; Bulut, Seval; Suleyman, Halis
Background: Acrylamide causes hepatotoxicity with the effect of oxidative stress and inflammatory processes. Carvacrol is a monoterpenic phenol with antioxidant and anti-inflammatory properties. Aims: To determine the effects of carvacrol on oxidative liver injury induced by acrylamide administration in rats. Methods: Rats were divided into three groups of six animals each: healthy group acrylamide group (ACR), and acrylamide + carvacrol group (TACR). First, carvacrol (50 mg/kg) was administered intraperitoneally to the CACR group. One hour later, acrylamide (20 mg/kg) was given orally to the ACR and CACR groups. This procedure was performed for 30 days, after which the animals were sacrificed. The malondialdehyde (MDA) and total glutathione (tGSH) levels, total oxidant (TOS) and total antioxidant status (TAS), tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), and nuclear factor kappa b (NF-κB) were measured in the excised liver tissues. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were determined in blood serum samples. Liver tissues were also examined histopathologically. Results: In the ACR group, malondialdehyde, TOS, ALT, AST levels, and NF-κB, IL-1β, and TNF-α levels were found to be high, and tGSH and total antioxidant status levels were low. In addition, diffuse degenerative changes and necrosis in hepatocytes, and moderate inflammation in the portal region were detected in the liver tissues of the ACR group. While carvacrol prevented the biochemical changes induced by acrylamide, it also alleviated the damage in the histological structure. Conclusion: Carvacrol may be used for liver damage caused by acrylamide.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2023Effect of anakinra, tocilizumab, and the combination thereof on bladder ischemia-reperfusion damage in albino Wistar-type rats; [Efecto de la anakinra, el tocilizumab y la combinación de ambos sobre el daño por isquemia-reperfusión vesical en ratas albinas tipo Wistar]
Bicer, Senol; Suleyman, Bahadir; Mammadov, Renad; Yavuzer, Bulent; Cicek, Betul; Altuner, Durdu; Coban, Taha A.; Suleyman, Halis
Several studies have reported that oxidative stress, and proinflam-matory cytokines such as tumor necrosis factor-alpha (TNF-α), interleukin-one beta (IL-1β), and interleukin-six (IL-6) are the main factors underlying bladder ischemia-reperfusion (I/R) damage. Anakinra and tocilizumab are known to be antioxidants and proinflammatory cytokine inhibitors. Our study aims to investigate if anakinra, tocilizumab, and the combination (ATC) thereof have a protective effect against oxidative and inflammatory bladder damage induced through the I/R procedure in rats, and evaluate by comparing these compounds. Male rats were divided into five groups: bladder sham-operation applied group (SG); bladder only I/R applied group (IRG); anakinra+bladder I/R applied group (AIR); tocilizumab+bladder I/R applied group (TIR); and ATC+bladder I/R applied group (ATIR). An atraumatic clamp was placed on the abdominal aorta of animals in all groups (except SG), and one hour of ischemia followed by two hours of reperfusion was performed. Our biochemical find-ings showed that anakinra and tocilizumab significantly inhibited the increase of oxidant malondialdehyde (MDA) and the decrease of antioxidants such as total glu-tathione (tGSH), superoxide dismutase (SOD), and catalase (CAT) in bladder tissue by I/R, both at the same levels. Furthermore, anakinra and tocilizumab significantly suppressed the I/R-associated increase of TNF-α, IL-1β, and IL-6 in bladder tissue. ATC was the one that best prevented the I/R-related increase in MDA, TNF-α, IL-1β, and IL-6 and the decrease in tGSH, SOD, and CAT in the bladder tissue. ATC was more beneficial than anakinra or tocilizumab alone in treating bladder I/R damage.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023The role of polymorphonuclear leukocytes in distant organ (lung) oxidative damage of liver ischemia/reperfusion and the protective effect of rutin
Olmez, Hasan; Tosun, Mustafa; Unver, Edhem; Cimen, Ferda Keskin; Arslan, Yusuf Kemal; Gulaboglu, Mine; Suleyman, Bahadir
Background. Ischemia/reperfusion (I/R) can cause damage to distant organs. Rutin is known to have antioxidant and anti-inflammatory properties, and inhibits cytokine and polymorphonuclear leukocyte (PMNL) infiltration. It may prevent the development of reperfusion injury. Objectives. This study aimed to examine the role of PMNLs in distant organ (lung) injury after a liver I/R procedure, and to evaluate the protective effects of rutin in rats using biochemical and immunohistochemical methods. Materials and methods. In this study, 18 Wistar albino male rats (255–275 g) were used. Experimental animals were divided into 3 groups: a liver I/R (LIR) group, a 50 mg/kg rutin+liver I/R (RLIR) group and a sham operation (SG) control group. Experimental results obtained from the RLIR group were compared with the LIR and SG groups. Results. Blood malondialdehyde (MDA) levels in the RLIR and SG groups were significantly lower compared to the LIR group (p < 0.001). Blood myeloperoxidase (MPO) activity in the RLIR and SG groups was significantly lower compared to the LIR group (p < 0.001). Total glutathione (tGSH) levels in the RLIR and SG groups were significantly higher compared to the LIR group (p < 0.001). Conclusions. Rutin can be used to prevent distant organ (lung) damage due to liver I/R. However, more extensive studies are needed on this issue. Copyright by Author(s).
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023Effects of benidipine, paracetamol, and their combination on postoperative and normal tissue pain thresholds
Bedir, Zehra; Ozkaloglu Erdem, Kezban Tuna; Doymus, Omer; Suleyman, Halis; Yavuzer, Bulent; Cicek, Betul; Altuner, Durdu; Mammadov, Renad; Yilmaz, Mehmet; Coban, Taha Abdulkadir
Introduction: In clinical practice, inadequate pain inhibition leads to increased morbidity and mortality. Increased intracellular calcium, oxidants, and proinflammatory cytokines are known to play a role in the pathogenesis of postoperative pain. Therefore, we investigated the analgesic effects of benidipine, paracetamol, and benidipine-paracetamol combination (BPC) on postoperative and normal pain thresholds in rats. Material and methods: Sixty-four male albino Wistar rats weighing 285–295 g were used. The without-incision rats were divided into 4 subgroups: healthy control, benidipine alone, paracetamol alone, and BPC. The scalpel-incision rats were divided into 4 subgroups: scalpel incision, scalpel incision + benidipine, scalpel incision + paracetamol, and scalpel incision + BPC. Paw pain thresholds of rats were measured using a Basile algesimeter. Biochemical analyses were performed on the paw tissues of 6 rats randomly taken from the experimental groups, each containing 8 rats. Rats were sacrificed immediately after the measurements. After the pain threshold tests were finished, the paw tissues were removed and malondialdehyde (MDA), total glutathione (tGSH), cyclooxygenase (COX), and interleukin-6 (IL-6) levels were measured. Results: There was no significant difference between the groups in paw pain threshold and measured biochemical parameters in rats without incision. The decrease in the pain threshold of the incised paw was also best prevented by BPC, followed by benidipine and then paracetamol. Furthermore, increases in scalpel-incised paw tissue MDA, COX-2, and IL-6 levels and the decrease in tGSH were significantly suppressed by benidipine and BPC, while paracetamol could only significantly inhibit the increase in IL-6 production. Conclusion: The combination of the L-type Ca2+ channel blocker benidipine and paracetamol (BPC) may provide potent analgesia. Our experimental results support that BPC may be useful in the treatment of severe pain that cannot be adequately inhibited by paracetamol.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023The role of Hippophae rhamnoides L. on 5-fluorouracil-ınduced oral mucositis in rats
Dilber, Muhammet; Suleyman, Bahadir; Mammadov, Renad; Suleyman, Zeynep; Yavuzer, Bulent; Gulaboglu, Mine; Erhan, Ertugrul; Salcan, Ismail; Suleyman, Halis
Current study aimed to research the effect of Hippophae rhamnoides (HRE) on potantial oral oxidative and inflammatory damage of 5-FU in rats. The rats were assigned to three groups; healthy (HG), 5-FU 100mg/kg (FUG) and HRE 50mg/kg +5-FU 100mg/kg (HRFU). The 5-FU was injected in the FUG group intraperitoneally. The HRFU was injected 5-FU at 100mg/kg IP one hour after the 50mg/kg HRE was given orally. Olive oil was used as a solvent for the HG. HRE was given to the rats three times a day for ten days. 5-FU was given one dose on the 1st, 3rd and 5th days. On the 10th day, the tissues removed from the animals were euthanized with high-dose anaesthesia and were macroscopically examined. The levels of the oxidant, antioxidant and proinflammatory cytokines were investigated.It was seen that HRE alleviated the symptoms of severe mucositis by antagonizing the effects of 5-FU on oxidant, antioxidant and proinflammatory cytokines such as malondialdehyde, total glutathione, superoxide dismutase, catalase, nuclear factor kappa-B and interleukin-6 in inner cheek and tongue tissue. These results recommend that HRE may be benefical in the cure of 5-FU-associated oral mucositis.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023Effect of Usnea longissima ethyl acetate extract on acute oxidative and inflammatory lung damage from Staphylococcus aureus infection in rats
Dabanlioglu, Bulent; Suleyman, Bahadir; Mammadov, Renad; Yavuzer, Bulent; Akyuz, Sumeyye; Akkas, Onder; Mokhtare, Behzad; Turumtay, Emine Akyuz; Altuner, Durdu; Coban, Taha Abdulkadir
The role of oxidants and proinflammatory cytokines in the pathogenesis of pneumonia caused by Staphylococcus aureus (S. aureus) has been demonstrated. The present study aims to investigate the protective effect of ethyl acetate extract (EtOAc) obtained from Usnea longissima (UL) against acute oxidative and inflammatory lung damage due to S. aureus infection in rats. Albino Wistar-type male rats were divided into three groups: Healthy (HG), S. aureus inoculated (SaG), and S. aureus inoculated + ULEtOAc administered (SUL). SaG (n = 6) and SUL (n = 6) group rats’ left nostrils (excluding HG) were inoculated with 0.1 ml bacterial mixture. After 24 hours, ULEtOAc (50 mg/kg) was administered orally to the SUL group, and the same volume of normal saline was administered orally to the HG (n = 6) and SaG groups. This procedure was performed once a day for seven days. Levels of oxidant and antioxidant parameters such as malondialdehyde (MDA) and total glutathione (tGSH), as well as pro-inflammatory cytokine levels such as nuclear factor-kappa B (NF-κB), tumor necrosis factor-alpha (TNF-α), interleukin-one beta (IL-1β), were measured in removed lung tissues. Tissues were also examined histopathologically. Biochemical results showed that ULEtOAc significantly suppressed the increase of MDA, NF-κB, TNF-α, and IL-1β levels and the decrease of tGSH caused by S. aureus in lung tissue. S. aureus inoculation caused severe mononuclear cell infiltration in interstitial areas, severe lymphoid hyperplasia in bronchial-associated lymphoid tissue and severe alveolar edema, histopathologically. Treatment with ULEtOAc had an attenuating effect on these histopathological findings. Experimental results from this study suggest that ULEtOAc may be beneficial in treating S. aureus-induced oxidative and inflammatory lung damage.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2023Protective Effect of Adenosine Triphosphate Against Bevacizumab-Induced Mandibular Bone Damage in Rats
Yalcin, Ertan; Bayramoglu, Zeynep; Mammadov, Renad; Altuner, Durdu; Bulut, Seval; Yavuzer, Bulent; Mokhtare, Behzad; Coban, Taha Abdulkadir; Suleyman, Halis; Suleyman, Bahadir
Bevacizumab is the anticancer drug used as an anti-angiogenic inhibitor of vascular endothelial growth factor (VEGF)-A. Bevacizumab causes toxicity by decreasing adenosine triphosphate (ATP) and increasing through reactive oxygen species (ROS) production. Male Albino Wistar rats were divided into 3 groups healthy (HG), bevacizumab administered (BVZ) and ATP+bevacizumab administered (ATP+BVZ). In the ATP+BVZ group, 25mg/kg ATP was injected between the mandibular gingiva and the bone. One hour after the application, 10 mg/kg bevacizumab was administrated intraperitoneally to the ATP+BVZ and BVZ groups. Two doses of bevacizumab were administered on the 1st and 15th days. ATP administration was continued once a day for 30 days. intoATP significantly prevented the increase in malondialdehyde (MDA) and total oxidant status (TOS) levels and the decrease in total glutathione (tGSH) and total antioxidant status (TAS) levels in the mandible bone tissue. ATP alleviated the histopathological damage associated with bevacizumab.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023Tocilizumab is effective in preventing ovarian injury induced by ischemia-reperfusion in rats
Uzel, Kemine; Lakhno, Igor; Turkler, Can; Kuzucu, Mehmet; Yazici, Gulce Naz; Mammadov, Renad; Suleyman, Bahadir; Kale, Ahmet; Suleyman, Halis
Ovarian torsion can be defined as the bending of the ovaries on the supporting ligament, disrupting both venous and arterial blood circulation. Insufficient blood flow causes ovarian tissue hypoxia and leads to ischemia. This study aimed to investigate whether tocilizumab has a protective effect on ischemia-reperfusion injury due to ovarian torsion in rats. Eighteen female Wistar albino rats were divided into three equal groups (Sham (SG), ischemia-reperfusion (OIR), and ischemia-reperfusion+tocilizumab (OIRT)). Degeneration, necrosis, vascular dilatation/congestion, interstitial edema, hemorrhage, and polymorphonuclear lymphocyte (PMNL) infiltration scores were significantly different between the groups (p=0.001 for all parameters). Moreover, the OIRT group had a significant improvement in these criteria compared to the OIR group (p<0.05). Additionally, there was a considerable difference between OIRT and OIR groups in the number of primordial, developing, and atretic follicles groups (p<0.05), while there was no difference in the number of corpus luteum (p=0.052). Stress markers or cytokines, such as MDA, tGSH, NF-κB, TNF-α, IL-1β, and IL-6, were significantly different between groups (p<0.05). Furthermore, a significant improvement was found in the measured variables when the OIRT group was compared with the OIR group (p<0.05). Tocilizumab may be an alternative option for treating ischemia-reperfusion injury due to ovarian torsion.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2023Protective Effect of Lycopene against Ethanol-induced Oxidative Kidney Damage in Rats
Yilmaz, Canan Tuz; Suleyman, Bahadir; Mammadov, Renad; Bulut, Seval; Gursul, Cebrail; Yazici, Gulce Naz; Gulaboglu, Mine; Suleyman, Halis
SUMMARY. Alcohol consumption can have dramatic effects on organs and tissue cells, with kidneys being some of the most affected organs. This study investigates the effect of lycopene on ethanol-induced oxidative kidney damage in rats. Albino Wistar rats were randomly divided into three groups. The first group served as the healthy group (HG), receiving distilled water as a solvent; the second group was the ethanol group (EtON), receiving only ethanol; the third group received both lycopene and ethanol (LEt). While ethanol ad-ministration increased malondialdehyde (MDA), total oxidant status (TOS), and oxidative stress index (OSI) levels, it caused a decrease in total glutathione (tGSH) and total antioxidant status (TAS) levels. Also, ethanol caused histopathological damage to the kidney. It has been observed that lycopene inhibits ethanol-induced biochemical and histopathological changes in kidney tissue. Lycopene inhibited the change in the oxidant-anti-oxidant balance towards oxidants under ethanol treatment and prevented ethanol-associated kidney oxidative damage.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2023Effect of Ketorolac and Nimesulide Combination on Surgical Pain, Stomach and Kidneys
Isik, Bahar; Suleyman, Bahadir; Mammadov, Renad; Bulut, Seval; Yavuzer, Bulent; Altuner, Durdu; Gulaboglu, Mine; Suleyman, Halis
Ketorolac is a nonsteroidal anti-inflammatory drug (NSAID). Cyclooxygenase-2 (COX-2) inhibition from the therapeutic effects of NSAIDs and COX-1 inhibition from toxic effects are held responsible. It is known that nimesulide has formed a protective effect by inhibiting the decrease in COX-1 activity and the increase in COX-2 activity. In this study animals were divided into healthy (HG), paw-scalpel incision (PSI) control (PSIC), PSI+nimesulide (PSIN), PSI+ketorolac (PSIK), and PSI+nimesulide+ketorolac (PSI+NKC) administered groups. Nimesulide was administered orally(50 mg/kg), and ketorolac was administered intra-peritoneally(5 mg/kg). Nimesulide and ketorolac showed similar analgesia (p = 0.194), while NKC provided the best analgesia (p < 0.001). Nimesulide inhibited the increase of oxidant and COX-2 in gastric tissue and increased antioxidant and COX-1 levels, protecting the stomach tissue from ketorolac damage(p < 0.01). In addition, NKC reduced the area and number of ketorolac-induced gastric ulcers in the stomach (p<0.001). Nimesulide also prevented ketorolac-induced renal dysfunction (p < 0.001).
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2023The Effect of Carvacrol on Oxidative and Inflammatory Liver Damage due to Staphylococcus aureus Infection in Rats
Dabanlioglu, Bulent; Suleyman, Bahadir; Mammadov, Renad; Coban, Taha Abdulkadir; Yazici, Gulce Naz; Akyuz, Sumeyye; Yavuzer, Bulent; Bulut, Seval; Suleyman, Halis
SUMMARY. Staphylococcus aureus is a bacterial pathogen that can cause oxidative stress. Carvacrol has anti-microbial, antioxidant, anti-inflammatory and hepatoprotective effects. This study evaluated the antibacterial effect of carvacrol and its effect on liver damage by S. aureus. The rats were divided into four groups: con-trol(HG), S. aureus injected (SaG), S. aureus + carvacrol (SaC), and S. aureus + cefazolin (SaCF). S. aureus was injected intraperitoneally (ip) to the SaG, SaC and SaCF groups. 24 h after the injection, carvacrol was given to the SaC group and cefazolin to the SaCF group as ip. Carvacrol and cefazolin administration was continued for 7 days. According to our antibiogram results, although the antibacterial effect of carvacrol was weaker than cefazolin, it prevented the increase of malondialdehyde (MDA), aminotransferase (ALT), aspar-tate aminotransferase (AST) and proinflammatory cytokines and the decrease of total glutathione (tGSH) better (p < 0.001). It also managed histopathological damage better than cefazolin. The results indicate that carvacrol may be helpful in the treatment of liver damage from infection.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023ANTI-INFLAMMATORY, ANALGESIC, AND GASTRIC TISSUE EFFECTS OF LACIDIPINE, ASPIRIN, AND THEIR COMBINATION
Isik, Bahar; Ates, Irem; Suleyman, Bahadir; Mammadov, Renad; Bulut, Seval; Gulaboglu, Mine; Ozcicek, Adalet; Suleyman, Halis
The increase in cyclooxygenase-2 (COX-2) activity and the decrease in COX-1 activity were associated with increased intracellular calcium. Lacidipine, a calcium channel blocker, can protect gastric tissue from aspirin-induced damage while potentiating the analgesic and anti-inflammatory properties of aspirin. The aim of this study was to investigate the anti-inflammatory, analgesic, and gastric tissue effects of using lacidipine and aspirin in combination and separately. (LA). Twenty-four rats were randomly divided into CG (carrageenan control), LCG (carrageenan + lacidipine), ACG (carrageenan + aspirin), and LACG (carrageenan + LA) groups. Lacidipine 4 mg/kg, aspirin 100 mg/kg were given orally to the animals on an empty stomach. Carrageenan was injected into the hind paws of rats to induce inflammation and pain. Paw volumes were measured at 0, 1, and 4 hours after carrageenan administration, and paw pain thresholds were measured at 1 and 4 hours. After euthanasia (thiopental sodium, 50 mg/kg), paw and gastric tissues were excised and biochemically analyzed. Gastric tissues were also examined macroscopically. In the paw tissues of animals receiving lacidipine, malondialdehyde (MDA) was lower than in the CG and AG, while total glutathione (tGSH) was higher (p<0.05). Lacidipine inhibited COX-2, but not COX-1 isoenzyme. LA—lacidipine, and aspirin—inhibited inflammation and hyperalgesia at 1 and 4 hours, respectively. Lacidipine prevented aspirin-induced COX-1 inhibition and gastric ulcer formation. Lacidipine combined with aspirin may be beneficial for initiating its anti-inflammatory activity early, achieving a potent analgesic effect, and preventing aspirin-induced gastric injury.
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