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- ArticleAçık Erişim2023Favipiravir-induced inflammatory and hydropic degenerative liver injury in rats
Bilici, Sami; Altuner, Durdu; Suleyman, Zeynep; Bulut, Seval; Sarigul, Cengiz; Gulaboglu, Mine; Altindag, Fikret; Ozcicek, Adalet; Gursul, Cebrail; Suleyman, Halis
Background. Favipiravir is very effective in the treatment of many viral infections, especially at high doses. It was used at such doses to treat coronavirus disease 2019 (COVID-19) during the pandemic. However, liver damage was reported in patients undergoing such treatment. Objectives. This study aimed to investigate the effects of low and high doses of favipiravir on the liver of rats, using biochemical and histopathological methods. Materials and methods. Wistar albino rats were allocated to one of 3 groups, namely a healthy group (HG), a 100 mg/kg favipiravir (FAV-100) group and a 400 mg/kg favipiravir (FAV-400) group. Favipiravir was administered orally at 100 mg/kg and 400 mg/kg doses to the FAV-100 (n = 6) and FAV-400 (n = 6) groups, respectively. Distilled water was administered orally (1 mL) using the same method to the HG (n = 6). This procedure was repeated twice a day for 1 week. At the end of this period, the animals were euthanized with a high dose of thiopental anesthesia (50 mg/kg) and their liver tissues were removed. Results. Favipiravir caused an increase in malondialdehyde (MDA), nuclear factor kappa B (NF-κB) and interleukin 6 (IL-6) levels in the liver tissue, as well as elevated alanine aminotransaminase (ALT) and aspartate aminotransferase (AST) levels in the blood. Moreover, favipiravir caused a decrease in total glutathione (tGSH), superoxide dismutase (SOD) and catalase (CAT) levels. In addition, severe edema, lymphocyte infiltration and hydropic degeneration were observed in the liver tissue of the FAV-400. Conclusions. High-dose favipiravir caused more significant oxidative and inflammatory damage in the liver tissue of rats than low-dose favipiravir.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2023The Effect of Carvacrol on 5-Fluorouracil-Induced Optic Neuropathy in Rats. Histopathological and Biochemical Analysis
Cicek, Ibrahim; Altuner, Durdu; Suleyman, Zeynep; Mammadov, Renad; Coban, Taha Abdulkadir; Gulaboglu, Mine; Yazici, Gulce Naz; Bayrakceken, Kemal; Suleyman, Halis
5-Fluorouracil (5-FU) is also known to cause ocular toxicity. The purpose of this study was to investigate the effects of Carvacrol upon possible 5-FU-induced optic nerve damage in rats biochemically and histopathologically. In this study totally, eighteen albino Wistar male rats were used. The rats were grouped under three categories as the healthy (HG) (n=6), 100 mg/kg 5-FU treated alone (FUG) (n=6) and 50 mg/ kg Carvacrol+100 mg/kg 5-FU (CFU) groups (n=6). Carvacrol at 50 mg/kg was injected intraperitoneally (ip) to the CFU group. The same volume of 0.9% NaCl was administered as the solvent ip to HG and FUG groups. One hour after administrating Carvacrol and solvent, 100 mg/kg 5-FU was ip injected to CFU and FUG groups. Carvacrol was administered once daily for 10 days. Three-dose 5-FU was administered on the 1st, 3rd, and 5th days as one dose each. On the 10th day, the animals were euthanised with high-dose anesthesia and optic nerve tissues were removed. Malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), and Interleukin (IL-6) levels were measured in optic nerve tissues. The tissues were also examined histopathologically. The results obtained from HG and CFU groups were compared with the FUG group. Significantly higher MDA and IL-6 levels (p < 0.001) and significantly lower (p < 0.001) tGSH, SOD, and CAT levels were determined in optic nerve samples of the FUG group rather than HG and CFU groups. In histopathological analyses, determined that astrocyte and oligodendrocyte nuclei were mostly darkly stained, the majority of astrocytes were hypertrophied and degenerated, and some oligodendrocytes were degenerated in the FUG group unlike HG and CFU groups. Moreover, the optic nerve tissue was determined to be vacuolized and intensely edematous, and blood capillaries were intensely dilated and congested. In conclusion, 5-FU caused optic neuropathy due to oxidative and inflammatory damage, and Carvacrol reduced this oxidative and inflammatory damage and histopathological changes in tissues. This revealed that Carvacrol was possibly to be efficient upon 5-FU-induced optic nerve toxicity.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2022Otoprotective Effect of Nimesulide: Biochemical and Histopathologic Evaluation
Kuduban, Ozan; Suleyman, Zeynep; Gulaboglu, Mine; Yazici, Gulce Naz; Suleyman, Halis
Background and Objective: It is known that nimesulide reduces the toxic effects of Non-Steroidal Anti-Inflammatory Drugs (NSAID), eliminating their inhibitory effects on cyclooxygenase-1 (COX-1) enzyme. COX-1 enzyme inhibition is thought to be responsible for aspirin ototoxicity therefore current study aimed to investigate the effect of nimesulide on aspirin ototoxicity in rats. Materials and Methods: Nimesulide 100 mg kg(-1) orally was administered to the nimesulide+aspirin group (NASA) and the same volume of distilled water as solvent was administered to the aspirin group (ASA) and the healthy (HG) group. Aspirin at a dose of 1000 mg kg(-1) was administered orally to the NASA and ASA group one hour after the administration of nimesulide and solvent. This procedure was repeated once a day for 7 days. Then, the animals were euthanized with a high dose of anaesthesia (50 mg kg(-1) thiopental sodium) and their cochlea and cochlear nerve tissues were removed. Results: Malondialdehyde (MDA) level was significantly higher and total glutathione (tGSH) and cyclooxygenase-1 COX-1 levels were significantly lower in the ASA group in which prominent histopathological damage was found in the cochlea and cochlear nerve, compared to the HG and NASA group. The COX-2 level was found to be almost the same in all the g roups. This indicates that aspirin reduces the COX-1 and tGSH levels and increases the MDA level, causing ototoxicity. Conclusion: Aspirin significantly decreased the COX-1 activity in the cochlea and cochlear nerve tissue, compared to the healthy and nimesulide group. Current study concluded that the co-administration of nimesulide and aspirin will increase the therapeutic effect of aspirin and reduces its side-effects.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2022Effect of Thymoquinone on Diclofenac-Induced Liver Injury
Ekinci, Bilge; Altuner, Durdu; Suleyman, Bahadir; Mammadov, Renad; Bulut, Seval; Suleyman, Zeynep; Gul, Mehmet A.; Ergul, Cetin; Suleyman, Halis
Background and Objective: Thymoquinone (TQ) is an active phenolic compound obtained from Nigella sativa L. It has anti-inflammatory and antioxidant activity by inhibiting the overproduction of certain inflammatory molecules and lipid peroxidation. TQ also has a hepatoprotective effect. This study, it was aimed to biochemically investigate the effect of TQ on diclofenac (DC)-induced liver damage in rats. Materials and Methods: The animals were divided into healthy control (HG), only diclofenac (DG), only thymoquinone (TQG) and diclofenac+thymoquinone (DTQG) groups. The DC was injected intraperitoneally at a dose of 25 mg kg(-1). The TQ was administered orally to the stomach at a dose of 20 mg kg(-1). This procedure was repeated once a day for 7 days. Results: Biochemical test results showed that TQ significantly prevented the increase in oxidant parameters and the decrease in antioxidants in liver tissue, which is formed by DC. Also, DC and TQ inhibited the increase of proinflammatory cytokines in liver tissue. The TQ also prevented the decrease of COX-1 activity by DC and increased the inhibition of COX-2. The TQ also significantly suppressed the elevation of liver function markers such as Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST) with DC. Conclusion: These findings indicated that the hepatotoxic effect of DC was due to oxidative stress and inhibition of cytoprotective structural COX-1 enzyme. It suggested that TQ may be useful in the treatment of DC-related liver injury.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2022Effect of tocilizumab on ischemia-reperfusion-induced oxido-inflammatory renal damage and dysfunction in rats
Erdem, Kezban Tuna Ozkaloglu; Bedir, Zehra; Kuyrukluyildiz, Ufuk; Tas, Hakan Gokalp; Suleyman, Zeynep; Bulut, Seval; Mendil, Ali Sefa; Sarigul, Cengiz; Unver, Edhem; Suleyman, Halis
Ischemia-reperfusion-induced (I/R) renal damage is a pathogenic process that starts with ischemia, then progresses through oxidative stress and inflammation. Tocilizumab (TCZ), a recombinant human monoclonal antibody produced against the IL-6 receptor, will be tested against renal I/R injury. TCZ is known to lower the levels of proinflammatory cytokines and oxidant mediators while raising the amounts of antioxidant molecules. Our purpose is to evaluate the biochemical and histological effects of TCZ against I/R-induced oxido-inflammatory kidney damage and dysfunction in rats. Animals were divided into 3 groups as renal I/R (RIR), I/R+ TCZ (IRT), and healthy group (HG). TCZ was administered at a dose of 8 mg/kg to the IRT group (n=6) of the animals, and distilled water as a solvent was administered intraperitoneally (ip) to the RIR (n=6) and HG (n=6) groups. Then, two hours of ischemia and six hours of reperfusion were applied to the left kidneys of IRT and RIR animals. TCZ significantly inhibited the increase in the levels of malondialdehyde (MDA), nuclear kappa B (NF-κB), tumour necrosis factor alpha (TNF-α), interleukin 1-β (IL-1β), IL-6, creatinine (Cr) and blood urea nitrogen (BUN) and decrease in total glutathione (tGSH) with I/R in renal tissue. TCZ also attenuated severe histopathological damage due to I/R in renal tissue. TCZ protected renal tissue from I/R-induced oxidative and inflammatory damage. These results indicate that TCZ may be useful in the treatment of renal I/R injury.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2022Effects of ketamine, thiopental and their combination on the rat liver: A biochemical evaluation
Bedir, Zehra; Ozkaloglu Erdem, Kezban Tuna; Ates, Irem; Olmezturk Karakurt, Tulay Ceren; Gursul, Cebrail; Onk, Didem; Kurt, Nezahat; Suleyman, Zeynep; Suleyman, Halis
Background. In the literature, it has been suggested that ketamine-related oxidative organ damage results from increased blood adrenaline level, and thiopental-related oxidative damage is caused by decreased adrenaline level, suggesting that ketamine-thiopental combination (KT) may be beneficial in reducing the hepatotoxic effect of ketamine. Objectives. To biochemically investigate the effects of ketamine, thiopental and KT on the liver in rats. Materials and methods. Male albino Wistar type rats received intraperitoneally (ip.) 30 mg/kg ketamine in the ketamine alone (KG) group (n = 6), 15 mg/kg thiopental in the thiopental alone (TG) group (n = 6), and 30 mg/kg ketamine + 15 mg/kg thiopental in the ketamine+thiopental (KTG) group (n = 6). The same volume of distilled water as solvent was given to the healthy (HG) animal group. This procedure was repeated once daily for 30 days. At the end of this period, the animals were killed by decapitation and their livers were removed. In liver tissue, malondialdehyde (MDA), total glutathione (tGSH), total oxidant status (TOS), total antioxidant status (TAS), tumor necrosis factor alpha (TNF-α), interleukin 1 beta (IL-1β), and interleukin-6 (IL-6) levels were measured. The IL-1β, IL-6, TNF-α, adrenalin (ADR), noradrenalin (NDR), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) levels were determined in blood samples taken from the tail veins. Results. In the group treated with ketamine and thiopental alone, MDA, TOS, IL-1β, IL-6, TNF-α, ADR, NDR, ALT, and AST levels were found to be high, and those of tGSH and TAS to be low. However, there was no significant change in the levels of these parameters in the KTG. Conclusions. These results indicate that oxidative stress and inflammation developed in the liver tissue of the group that used ketamine and thiopental alone, suggesting that the KT form may be safer in terms of toxicity in the clinical usage.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2022The Effect of Thiamine Pyrophosphate and Tocilizumab Alone and in Combination upon Ethanol-Induced Peripheral Neuropathy and Neuropathic Pain in Rats
Ekinci, Bilge; Suleyman, Bahadir; Mammadov, Renad; Cankaya, Murat; Yazici, Gulce Naz; Suleyman, Zeynep; Dal, Cagatay Nusret; Ergul, Emine Esra; Onk, Didem; Suleyman, Halis
The aim of the study to determine the effects of thiamine pyrophosphate (TPP) and tocilizumab (TCZ) either alone or in combination on ethanol-induced neuropathic pain and peripheral neuropathy in rat nerve tissues, biochemically and histopathologically. Albino Wistar male rats were divided into five groups as the healthy (HG), ethanol alone (EtOH), TPP + ethanol (TPE), TCZ + ethanol (TCE), and TPP + TCZ + ethanol (TPTE) groups. TPE (n = 6), TCE (n = 6) and TPTE (n = 6) groups were injected with 20 mg/kg TPP, 8 mg/kg TCZ, and 20 mg/kg TPP+8 mg/kg TCZ intraperitoneally (respectively). Distilled water were given the HG (n = 6) and EtOH (n = 6) groups. One hour after the drugs and distilled water were administered, 35% ethanol was administered orally by lavage at a 5 g/kg dose to the TPE, TCE, TPTE, and EtOH groups. Paw pain thresholds of animals were measured using Basile Algesimeter. TPTE, which suppressed the decrease of the pain threshold with ethanol more significantly than TPP and TCZ. The best inhibitor of MDA increase and inhibit the reduction of tGSH by etanol was listed as TPTE > TPE > TCZ. The potency of suppressing the increase in cytokine production with ethanol was listed as TPTE > TCE > TPE. In conclusion, the results indicate that TPP+TCZ may be more beneficial than TPP and TCZ alone in treating ethanol-related neuro-pathic pain.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2022Effect of Thymoquinone on Everolimus-Induced Oral Toxicity in Rats. A Macroscobic and Biochemical Evaluation
Dilber, Muhammet; Salcan, Ismail; Suleyman, Bahadir; Mammadov, Renad; Altuner, Durdu; Aggul, Ahmet G.; Esen, Mehtap; Bulut, Seval; Suleyman, Zeynep; Yavuzer, Bulent
Everolimus is a drug within the class of mammalian target of rapamycin inhibitors (mTOR). The most widespread side effect observed is oral mucositis.Thymoquinone is a phenolic compound obtained antioxidant and anti-inflammatory activities. The aim of this study is to investigate the protective effect of thymoquinone against oral toxicity that may develop due to everolimus in rats. Albino Wistar male rats were divided into healthy(HG), everolimus(EVR), thymoquinone(TQG) and thymoquinone+everolimus(TQE) groups. Thymoquinone (20 mg/kg) was administered to the TQG and TQE groups orally. One h after, TQE and EVR groups were given everolimus to (2 mg/kg) the stomach. This procedure was repeated once a day for four weeks. After 4 weeks, animals were euthanized, tongue and cheek tissues were examined biochemically and histopathologically. Thymoquinone prevented the increase of oxidant and proinflammatory cytokines and the decrease of antioxidants in the tongue and cheek tissues of animals, and also reduced the histopathological damage.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2022The effect of taxifolin on experimental testicular ischaemia reperfusion injury in rats. A biochemical and histopathological analysis; [Efecto de la taxifolina en la lesión experimental testicular por reperfusión-isquemia en ratas. Análisis bioquímico e histopatológico]
Bedir, Fevzi; Kocaturk, Huseyin; Ozgeris, Fatma Betul; Yazici, Gulce Naz; Suleyman, Zeynep; Suleyman, Halis
Objectives: The aim of the study is to investigate the protective effect of taxifolin (3,5,7,3,4-pentahydroxy flavanone), a strong antioxidant, against testicular I/R injury in rats biochemically and histopathologically. Materials and methods: 50 mg/kg taxifolin was administered to taxifolin + testicular torsion–detorsion (TTTD, n-10) group of Albino Wistar male rats by oral gavage. Distilled water .5 ml as a solvent was administered to testicular torsion–detorsion (TTD, n-10) and Healthy Control (SG, n-10) groups using the same method. An hour after the administration of taxifolin and distilled water, anaesthesia (ketamine 60 mg/kg) was administered to all animal groups. TTD and TTTD group animals were subjected to testicular torsion at 720 degrees for four hours during anaesthesia. At the end of this period, testicular detorsion was applied and perfusion was allowed for four hours. Sham operation was applied to SG group. Results: Our biochemical experiment results showed that the amount of malondialdehyde (MDA) in testicular tissue of TTD group presented a significant increase compared to SG and TTTD groups whereas total glutathione (tGSH) and superoxide dismutase (SOD) levels decreased. In addition, while TTD group presented severe histopathological damage in germinal epithelium cell and seminiferous tubule, mild damage was observed in TTTD group. Conclusions: The results of our experiment indicate that taxifolin could be useful in the treatment of testicular I/R damage.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2022Effect of taxifolin on methotrexate-induced oxidative and inflammatory oral mucositis in rats: biochemical and histopathological evaluation
Bayramoglu, Zeynep; Mokhtare, Behzad; Mendil, Ali Sefa; Coban, Taha Abdulkadir; Mammadov, Renad; Bulut, Seval; Suleyman, Zeynep; Suleyman, Halis
The role of oxidative stress, as well as inflammation in the pathogenesis of methotrexate (MTX)-induced oral mucositis, is a known fact. The anti-inflammatory, antitumor, antimicrobial, and antioxidant properties of taxifolin—the effect we tested against MTX-induced oral mucosal damage—are well known. Objective: Evaluating biochemically and histopathologically the effects of taxifolin on methotrexate-induced oral mucosal damage in rats. Methodology: In the taxifolin+MTX (TMTX) group, 50 mg/kg taxifolin was orally administered to rats by gavage. In the MTX and healthy (HG) groups, normal saline was applied to rats as solvent by the same method. One hour after administration of taxifolin and solvent, 5 mg/kg MTX was orally administered to rats in the MTX and TMTX groups. Taxifolin and methotrexate were administered once a day for 30 days. Macroscopic, biochemical, and histopathological evaluations were performed on the inner cheek and tongue tissues of rats. These parts were removed after rats were killed with a high-dose anesthesia. Results: Taxifolin with MTX prevented the increase in oxidant and pro-inflammatory parameters, such as malondialdehyde (MDA), tumor necrosis factor alpha (TNF-α), interleukin 1 beta (IL-1β), interleukin 6 (IL-6), on the inner cheek and tongue tissues of rats. Moreover, taxifolin antagonized the decrease in total glutathione (tGSH). Taxifolin decreased MTX-induced histopathological damage. Conclusion: These findings suggest that taxifolin may be useful to treat MTX-associated oral mucositis.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2022Molecular Mechanism and Pathogenesis-Oriented Treatment of Antipsychotic Drug-Induced Infertility in Female Rats
Ozyurt, Ramazan; Suleyman, Halis; Dinc, Kemal; Yildiz, Sevler; Coban, Taha Abdulkadir; Bulut, Seval; Suleyman, Zeynep; Altuner, Durdu; Keskin, Deha Denizhan; Keskin, Seda
Typical and atypical antipsychotics may cause subfertility through oxidative stress in reproductive cells and endocrine axis. This experimental study was designed to determine the effects of adenosine triphosphate (ATP) treatment on oxidative stress markers and hyperprolactinemia in subfertility due to antipsychotics. A total of 54 Albino Wistar female rats were divided into nine groups as the healthy (HG), haloperidol (HPL), olanzapine (OLZ), clozapine (CLZ), aripiprazole (ARZ), ATP+haloperidol (ATP+HPL), ATP+olanzapine(ATP+OLZ), ATP+clozapine (ATP+CLZ) and ATP+aripiprazole (ATP+ARZ) groups. ATP was injected intraperitoneally (ip) at a dose of 25 mg/kg to animals in ATP+HPL, ATP+OLZ, ATP+CLZ, and ATP+ARZ groups. HG, HPL, OLZ, CLZ, and ARZ groups were given the same volume of distilled water via ip. One hour after administration of drugs and solvent, haloperidol (2 mg/kg) for ATP+HPL and HPL groups, olanzapine (2 mg/kg) for ATP+OLZ and OLZ groups, clozapine (20 mg/kg) for ATP+CLZ and CLZ groups and aripiprazole (3 mg/kg) for ATP+ARZ and ARZ groups was given orally to by gavage. This procedure was repeated once a day for 15 days. After this period, blood samples were taken for oxidant, antioxidant and prolactin measurement and the animals were kept with mature male rats for one month for breeding. The degree of oxidative stress, hyperprolactinemia and subfertility caused by antipsychotics were found to be haloperidol, olanzapine, clozapine, aripiprazole, in order of frequency. ATP administration reversed the increase in oxidants markers and the decrease of antioxidants significantly. ATP administration prevents subfertility due to antipsychotic drugs.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2022The Effect of Adenosine Triphosphate, Benidipine, Sugammadex, and their Combinations upon Cardiac Damage after Temporary Clamping of the Abdominal Aorta in Rats
Emir, Izzet; Altuner, Durdu; Gulaboglu, Mine; Coban, Taha Abdulkadir; Suleyman, Bahadir; Mammadov, Renad; Ozkaraca, Mustafa; Suleyman, Zeynep; Karakurt, Tulay Ceren Olmezturk; Suleyman, Halis
The aim of our study was to biochemically and histopathologically investigate the protective effect of adenosine triphosphate (ATP), benidipine, sugammadex and (ATP + benidipine + sugammadex) ABS against cardiac damage induced by abdominal clamping and unclamping (ACU) procedure in rats. Rats utilized were divided into six groups as Healthy (HG), Abdominal ACU (AACC), ATP + AACU (ATPG), Benidipine + AACU (BNDG), Sugammadex + AACU (SDXG), ABS + AACU (ABSG). One hour before anesthesia, ATP (25 mg/kg; ip) was administered to the ATPG group, Benidipine (4 mg/kg orally) was administered to the BNDG group, Sugammadex (4 mg/kg ip) was administered to the SDXG and ABS was administered with this specified dose and method to the ABSG group. Distilled water as a solvent was injected to AACC and HG groups. One hour after, laparotomy was performed to the rats, and the abdominal cavity was reached. Ischemia was provided for one hour and then reperfusion for 2 h placing an atraumatic clamp on the suprarenal abdominal aorta of the animals in all groups (except from the HG). The heart tissues extracted from the killed animals were examined biochemically and histopathologically. ATP and benidipine suppressed the increase of oxidant and proinflammatory cytokines better than sugammadex. The ABSG group was found to be almost the same as the healthy group biochemically and histopathologically (p > 0.05). ABS drug form alone was possible to be much more beneficial rather than ATP, benidipine and sugammedex in the treatment of cardiac damage induced by AACU procedure.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2022Effect of Rhodiola rosea Root Extract on Stress-Induced Gastric Ulcer in Rats
Isik, Bahar; Altuner, Durdu; Ozcicek, Fatih; Ezmeci, Talat; Aggul, Ahmet G.; Suleyman, Bahadir; Bulut, Seval; Suleyman, Zeynep; Yavuzer, Bulent; Suleyman, Halis
Stress can lead to stomach ulcers. Oxidative stress and inflammation are involved in the pathogenesis of stress ulcers. Rhodiola rosea root extract (RRE) has antistress, anxiolytic, antioxidant and anti-inflammatory effects. In this study, the effect of RRE on gastric ulcers induced by forced immobilization method (FIM) was investigated. Albino Wistar male rats were divided into healthy (HG), control (SC), stress+RRE (SRE) and stress+famotidine (SFM) groups. RRE was given to the rats’ SRE group, and famotidine to the SFM group was administered orally. One hour after, the animals in the SRE, SFM and SC groups were subjected to stress with FIM for one hour once a day. This procedure continued for seven days. After this period, the animals were euthanized. Stomach tissues were examined macroscopically and biochemically. RRE reduced the number and area of stress-induced ulcers and biochemical changes in the gastric tissues of animals better than famotidine.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2022Effect of thiamine pyrophosphate on cyclophosphamide-induced oxidative ovarian damage and reproductive dysfunction in female rats
Ozer, Mehmet; Ince, Sefa; Gundogdu, Betul; Aktas, Mehmet; Uzel, Kemine; Gursul, Cebrail; Suleyman, Halis; Suleyman, Zeynep
Background. Cyclophosphamide is a drug used in various types of cancer. It can cause oxidative and inflammatory ovarian damage and infertility. Thiamine pyrophosphate (TPP) to be investigated for its effect on cyclophosphamide-induced ovarian damage and reproductive dysfunction in the present study is the active metabolite of thiamine. It has been shown that TPP protects organs and tissues from oxidative stress and proinflammatory cytokine damage. Objectives. To investigate the effect of TPP against the ovarian damage and reproductive dysfunction caused by cyclophosphamide in rats. Materials and methods. Albino Wistar type female rats were divided into healthy control (HG), cyclophosphamide (CYC) and TPP + cyclophosphamide (TPPC) groups (for each group, n = 12). Thiamine pyrophosphate at a dose of 25 mg/kg was injected intraperitoneally (ip.) in the TPPC group, and 0.9% NaCI solution was injected ip. in the CYC and HG groups. One hour after the injection, 75 mg/kg of cyclophosphamide was administered ip. in the TPPC and CYC groups. This procedure was repeated once a day for 30 days. At the end of this period, 6 rats from each group were euthanized with a high dose of anesthetic (50 mg/kg of sodium thiopental). Biochemical and histopathological examinations were performed on the extracted ovarian tissue. The remaining animals were kept in the laboratory with mature male rats for 2 months for reproduction. Results. Thiamine pyrophosphate significantly decreased the cyclophosphamide-induced increase in the levels of the oxidant parameter malondialdehyde (MDA), proinflammatory nuclear factor kappa B (NF-κB), tumor necrosis factor alpha (TNF-α), and interleukin 1 beta (IL-1β). In addition, TPP decreased the severe histopathological damage associated with cyclophosphamide in the ovarian tissue and prevented infertility. Conclusions. Our experimental results have suggested that TPP could be beneficial in the treatment of cyclophosphamide-induced ovarian injury and infertility.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2021Biochemical and Histopathological Evaluation of Sunitinib Effect on Ovarian Injuries by Ischemia-Reperfusion in Rats
Ince, Sefa; Ozer, Mehmet; Kadioglu, Berrin Goktug; Kuzucu, Mehmet; Gundogdu, Betul; Gursul, Cebrail; Suleyman, Zeynep; Suleyman, Halis
Background and Objective: Interleukin 1 beta (IL-1 beta) and tumour necrosis factor-alpha (TNF-alpha) as the proinflammatory cytokines, whose production increases during reperfusion after ischemia (I/R), were reported to produce the Reactive Oxygen Species (ROS). It was reported that sunitinib has proinflammatory cytokine expression suppression and antioxidant effects. The study aims to investigate the effects of sunitinib on ovarian injuries created by I/R in albino Wistar female rats biochemically and histopathologically. Materials and Methods: Animals divided into 3 groups as sunitinib+ovarian tissues (SOIR), I/R ovarian tissues (OIR) and Sham operation (SG) groups. There were ischemia and reperfusion procedures for 2 hrs on the right ovaries of the OIR and SOIR group rats. Results: The Nuclear Factor kappa B (NF-kappa B), Tumour Necrosis Factor-alpha (TNF-alpha), Malondialdehyde (MDA) and Interleukin 1 beta (IL-1 beta) levels in the OIR group's ovarian tissue were high whereas their total glutathione (tGSH) level was found to be low compared to the SOIR and SG group. While distinct histopathologic injuries were seen in the ovarian tissue of the OIR group, histopathologic injuries in the SOIR group were mild. Conclusion: Sunitinib prevented the oxidant and proinflammatory cytokine increase and antioxidant decrease related to I/R. It is concluded that sunitinib might be used for the treatment of ovarian I/R injuries.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2021The effect of adenosine triphosphate on propofol-induced myopathy in rats: A biochemical and histopathological evaluation
Erdem, Kezban Tuna Ozkaloglu; Bedir, Zehra; Ates, Irem; Kuyrukluyildiz, Ufuk; Coban, Taha Abdulkadir; Yazici, Gulce Naz; Arslan, Yusuf Kemal; Suleyman, Zeynep; Suleyman, Halis
Propofol infusion syndrome characterized by rhabdomyolysis, metabolic acidosis, kidney, and heart failure has been reported in long-term propofol use for sedation. It has been reported that intracellular adenosine triphosphate (ATP) is reduced in rhabdomyolysis. The study aims to investigate the protective effect of ATP against possible skeletal muscle damage of propofol in albino Wistar male rats biochemically and histopathologically. PA-50 (n = 6) and PA-100 (n = 6) groups of animals was injected intraperitoneally to 4 mg/kg ATP. An equal volume (0.5 ml) of distilled water was administered intraperitoneally to the P-50, P-100, and HG groups. One hour after the administration of ATP and distilled water, 50 mg/kg propofol was injected intraperitoneally to the P-50 and PA-50 groups. This procedure was repeated once a day for 30 days. The dose of 100 mg/kg propofol was injected intraperitoneally to the P-100 and PA-100 groups. This procedure was performed three times with an interval of 1 days. Our experimental results showed that propofol increased serum CK, CK-MB, creatinine, BUN, TP I, ALT, AST levels, and muscle tissue MDA levels at 100 mg/kg compared to 50 mg/kg and decreased tGSH levels. At a dose of 100 mg/ kg, propofol caused more severe histopathological damage compared to 50 mg/ kg. It was found that ATP prevented propofol-induced muscle damage and organ dysfunction at a dose of 50 mg/kg at a higher level compared to 100 mg/kg. ATP may be useful in the treatment of propofol-induced rhabdomyolysis and multiple organ damage.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2021Valeriana officinalis is Effective on Stress-Induced Infertility, Delayed Maternity, and Intrauterine Growth Restriction of the Fetus in Female Rats
Uzel, Kemine; Turkler, Can; Mammadov, Renad; Yazici, Gulce Naz; Arslan, Yusuf Kemal; Coban, Taha Abdulkadir; Suleyman, Zeynep; Gursul, Cebrail; Suleyman, Halis
SUMMARY. Infertility is a tragic option for reproductive-aged women. About 20 % of lost ability to conceive are caused by unexplained etiology. Some of these cases are linked with psychological stress. It can cause depression and anxiety, and the latter can cause infertility. The therapy with sedative, antidepressant, anxi-olytic and antioxidant effects may be beneficial in preventing infertility caused by psychological disorders and maintaining normal intrauterine physical development of the fetus. The aim of our study is to investi-gate the protective effect of Valeriana plant root extract against stress-induced reproductive disorders. The protective effect of Valeriana plant root extract against infertility and delayed maternity was investigated in a stress model induced by the forced immobilization method in albino Wistar female rats and compared with ascorbic acid. Valeriana officinalis plant root extract was effective against psychological stress-related infertility and delayed maternity, ascorbic acid was found to be ineffective. This information suggests that psychological stress-related ovarian damage, infertility, delayed maternity, and great obstetric syndromes are based on the role of psychological disorders such as anxiety and depression rather than oxidative stress. Our experimental results indicate that Valeriana officinalis plant root extract can be useful in the treatment of stress-related ovarian damage and reproductive disorders.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2021The effect of adenosine triphosphate on oral mucosity in rats due to sunitinib administration
Dilber, Muhammet; Salcan, Ismail; Bayram, Rana; Suleyman, Esma; Yildirim, Ugur; Sonmez, Faik; Bakan, Ebubekir; Coban, Taha Abdulkadir; Suleyman, Zeynep; Suleyman, Halis
Sunitinib, a common drug used in cancer treatment, is a multi-targeted tyrosine kinase in-hibitor. It is argued that sunitinib-associated oral mucositis is caused by VEGF inhibition. Previous research has documented that ATP allows for wound healing through an increase in VEGF levels and pro-vides an antioxidant effect. The aim of our study was to biochemically analyze whether adenosine triphos-phate (ATP) offers any protective effects against potential oral oxidative mucosal damage due to sunitinib use in male albino Wistar rats. ATP was injected ip at 25 mg/kg dose to sunitinib + ATP(SAT) (n=8) group. Normal saline (0.9% NaCl) was applied in the same route to the sunitinib-alone(SUN) (n=8) and healthy (HG) (n=8) groups as the solvent. One hour after ATP and 0.9% NaCl were administered, oral de-livery of 25 mg/kg sunitinib was given to the SAT and SUN groups, once a day for four weeks. While MDA, TOS and 8-OHdG levels were significantly higher (p < 0.001) compared to the healthy and ATP group in the oral cavity tissues of subjects treated with sunitinib, tGSH and TAS were significantly lower (p < 0.001). Our experimental results show that ATP protects tongue and cheek tissues of rats from oxida-tive damage of sunitinib, indicating that ATP may be beneficial for sunitinib-related oral mucosal injury.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2020The effect of desloratadine on ischemia reperfusion induced oxidative and inflammatory renal injury in rats
Kocaturk, Huseyin; Bedir, Fevzi; Altay, Mehmet Sefa; Bakan, Ebubekir; Suleyman, Bahadir; Yazici, Gulce Naz; Sunar, Mukadder; Suleyman, Zeynep; Suleyman, Halis
Purpose: To examine the effect of desloratadine on kidney ischemia-reperfusion (I/R) injury in albino Wistar male rats using biochemical and histopathological methods. Methods: The treated with ischemia–reperfusion + 5 mg/kg desloratadine (IRD) group (n–6) was given 5 mg/kg desloratadine by gavage orally, and applied renal ischemia-reperfusion (BIR) group (n–6) and control (SG) group undergoing Sham operation (n–6) rats were given distilled water as solvent one hour before ketamine anesthesia. During the anesthesia period, ischemia was induced for 2 h unilaterally in the left kidney of all rats followed by reperfusion for 6 h. The kidneys of the SG group had sham operation without any intervention. Results: Our biochemical test results showed that malondialdehyde (MDA), nuclear factor kappa (NF-κB), tumor necrosis factor alpha (TNF-α), interleukin one beta (IL-1β), creatinine, and blood urea nitrogen (BUN) levels were significantly increased in the BIR group compared to the healthy control and IRD groups treated with desloratadine. Histopathological results were revealed tubular dilatation, tubular necrosis, loss of brushy margins, cast formation, and apoptotic bodies in tubular epithelial cells in the BIR group. There were no histopathological findings except for the swelling of tubule epithelial cells and the accumulation of proteinous material in some tubule lumens in renal tissue of desloratadine-treated rats. Conclusions: Experimental results suggested that desloratadine may be useful in the treatment of renal I/R injury.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2020Effect of desloratadine on oxidative and inflammatory ovarian ischemia-reperfusion injury in female rats biochemical and histopathological evaluation
Kadıoglu, Berrin Goktug; Gundogdu, Betul; Kurt, Nezahat; Suleyman, Halis; Suleyman, Zeynep
OBJECTIVE: To examine the effect of desloratadine on ovarian ischemia-reperfusion (I/R) injury in rats by biochemical and histopathological methods. STUDY DESIGN: I/R was induced in the right ovaries after distilled water was given to a group of female albino Wistar rats in this study. 15 mg/kg desloratadine was orally administered to another group and I/R was induced in their ovaries, whereas I/R was not induced in the sham operation group. After 2 hours of ischemia and 2 hours of reperfusion, malondialdehyde (MDA), total glutathione (tGSH), nuclear factor-κB (NF-κB), interleukin 1 beta (IL-1β), and tumor necrosis factor alpha (TNF-α) levels were measured in ovarian tissues extracted from the body of the animals sacrificed with a high dose of ketamine (120 mg/kg). Tissues were also evaluated histopathologically. RESULTS: It has been revealed that desloratadine significantly inhibited the increase of I/R-associated MDA, NF-κB, IL-1β, and TNF-α levels in the ovarian tissues of animals and the decrease in tGSH levels. In addition, desloratadine decreased severe I/R-associated histopathological damage. CONCLUSION: Our experimental results have shown that desloratadine is beneficial in terms of protecting ovarian tissue against I/R injury.
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