Yazarlar
- Suleyman, Halis(167)
- Mammadov, Renad(95)
- Türkeş, Cüneyt(84)
- Aydin, Sonay(83)
- Altuner, Durdu(74)
- Suleyman, Bahadir(68)
- Isik, Arda(67)
- Beydemir, Şükrü(66)
- Kantarci, Mecit(64)
- Kurt, Nezahat(57)
- Demir, Yeliz(54)
- Bulut, Seval(51)
- Arslan, Yusuf Kemal(50)
- Sofu, Hakan(50)
- Coban, Taha Abdulkadir(49)
- Karakeçili, Faruk(47)
- Altay, Ahmet(46)
- Huyut, Mehmet Tahir(46)
- Aydin, Suleyman(45)
- Türkmenoğlu, Burçin(45)
- Kuzucu, Mehmet(40)
- Yazici, Gulce Naz(40)
- Peker, Kemal(39)
- Algul, Oztekin(38)
- Cicek, Betul(37)
- Mertoglu, Cuma(36)
- Camurcu, Yalkin(35)
- Suleyman, Zeynep(34)
- Bozkurt, Aliseydi(32)
- Mendil, Ali Sefa(32)
- Gunay, Murat(31)
- Gözcü, Sefa(31)
- Köksal, Ekrem(31)
- Yuca, Hafize(31)
- Dilek, Esra(30)
- Karakurt, Papatya(30)
- Ozcicek, Fatih(30)
- Süleyman, Halis(30)
- Onk, Didem(29)
- Ucpunar, Hanifi(29)
Yayınlar
- MakaleAçık Erişim2024The Association of Neuroendocrine Differentiation with MicroRNA 21 and MicroRNA let7f Expression and the Clinicopathological Parameters in Primary Invasive Breast Carcinomas with Neuroendocrine Features
Usul, Gamze; Kelten Talu, Esra Canan; Yılmaz, İsmail; Issın, Gizem Narlı; Bektaş, Sibel; Can Trabulus, Didem
MiRNAs have been reported as biomarkers with diagnostic, prognostic, and predictive value for many different diseases. Therapeutic agents targeting some miRNAs are currently being developed. We aimed to compare BC-NEFs (carcinoma of the breast with neuroendocrine features) with IDC (invasive ductal carcinoma) cases without neuroendocrine features in terms of the level of miRNA expression known to show the oncogenic (miR-21) and tumor-suppressor effects (miR-let7f) and the clinicopathological features. A total of 29 patients with a diagnosis of BC-NEFs (15 cases with neuroendocrine differentiation >50% of the whole section of tumor and 14 cases with neuroendocrine differentiation 10–50% of the tumor) and 30 patients with a diagnosis of IDC (no neuroendocrine differentiation) were retrospectively re-evaluated. Expression levels of miR-21 and miR-let7f were determined by the qRT-PCR method in paraffin tissue blocks. MiR-21 expression was significantly higher in the IDC group than in the group with BC-NEFs. miR-let7f expression was significantly lower in the group with BC-NEFs compared to the IDC group. A high expression level of miR-21 was found to be associated with progesterone receptor (PR) negativity. Our findings show that the presence of NEFs in breast carcinomas makes a significant difference in the expression levels of the investigated oncogenic (miR-21) and tumor-suppressor (miR-let7f) miRNAs. These findings suggest that miRNAs may be a potential biomarker in BC-NEFs and would benefit from targeted therapy.
- MakaleYalnız künye2024Are micro-RNA 21 and 143 indicative as prognostic biomarkers in dedifferentiated endometrial adenocarcinoma?
Calli, Aylin Orgen; Kurt, Kerem; Narli, Gizem; Kocabey, Duygu Unal; Yilmaz, Alpay; Ocal, Irfan; Yigit, Seyran; Yilmaz, Ismail
Aim: Dedifferentiated endometrial adenocarcinoma (DEAC) is a rare, aggressive subtype, accounting for 2% of all endometrial cancers. Poor survival in DEAC prompts the need for effective treatment modalities through better prognostic classification. MicroRNAs (miRNA) have essential roles in tumor angiogenesis, which might enable their use as novel biomarkers. In this study, we aimed to reveal the relationship between the expression of miRNA-21 and miRNA-143, which are associated with angiogenesis, and the prognosis of DEAC. Method: The study included six cases diagnosed with DEAC. The expression levels of miRNA-21 and miRNA-143 were detected by quantitative real-time PCR. Microvascular density (MVD) was measured by CD34 staining. All data and effects on survival were compared for statistical significance. Results: Six cases diagnosed with DEAC were included in the study. The percentage of undifferentiated components ranged from 50 to 90%. The second component of differentiated carcinoma was detected as endometrioid (3/5 grade I, 1/5 grade II, 1/5 grade III) in five cases and serous in one case. The mean MVD was 27 (range 17–44, SD 9.4). In three cases, miRNA-21 expression was down-regulated in neoplastic areas compared to non-neoplastic areas. On the contrary, it was found to be up-regulated in the remaining three cases. MiRNA-143 expression decreased in four cases and increased in two cases. Conclusions: Based on these findings, we found a significant irregular expression of miRNA-21 in DEACs. As in other cancers, angiogenesis is significantly associated with survival in DEACs. This study provides initial data for revealing possible implications of miRNAs as prognostic indicators in DEAC.
- MakaleYalnız künye2024The effect of social support and optimism on depression in mothers of premature babies hospitalized in neonatal intensive care unit
Köse, Semra; Ayran, Gülsün
Background: This study was carried out to investigate the effect of social support and optimism upon the level of depression among mothers of premature babies hospitalized in the neonatal intensive care unit. Methods: This descriptive and relationship-seeking study was carried out between February and October 2022 with mothers of premature babies hospitalized in the Neonatal Intensive Care Clinic of a university hospital. The study was carried out with 152 mothers who met the inclusion criteria. The data were interpreted using percentage, mean, Pearson correlation, and regression analysis. Results: In the study, it was found that there was a statistically significant positive correlation between mothers' perception of social support and their level of optimism. A statistically significant negative correlation was found between the level of depression and the level of optimism. The model established to determine the effect of social support and optimism on the level of depression was found to be statistically significant. Conclusions: In conclusion, it was found that mothers of premature infants with social support who were hospitalized in the neonatal intensive care unit had an increased level of optimism, and this optimism reduced the level of depression among the mothers.
- MakaleAçık Erişim2024Comparative content analysis and biological activity studies of fatty and essential oils from some garlic products sold in Turkish community pharmacies, natural, and fermented garlic
Deniz, Elif Ulutaş; Yuca, Hafize; Aydın, Bilge; Öztürk, Gözde; Çoban, Furkan; Göger, Gamze; Demirci, Betül; Karakaya, Songül
Garlic (Alliaceae), an annual herb, is renowned not only for its distinctive flavor but also for its extensive therapeutic applications in managing various ailments and health conditions. In this study, garlic products identified as the best-selling items in Turkish pharmacies for various purposes were compared with garlic grown under standard conditions in terms of chemical composition and antidiabetic, anticholinesterase, antimicrobial, and antioxidant properties. Three of garlic samples were prepared by researchers. According to survey results, the most commonly sold garlic-related products in pharmacies are black garlic extract tablets (4), capsules (5), garlic oil (6), garlic oil pearls (7), and fermented garlic (8). Diallyl disulfide (DADS) was identified as a predominant compound in sampled oils, ranging from 4.9% to 48.6%. Another noteworthy finding is the identification of allyl methyl disulfide and allyl methyl trisulfide as major components in sampled oils, with concentrations spanning from 2.9% to 9.8% and 0.4% to 17.5%, respectively. In both 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS·+) and 2,2-diphenyl-1-picrylhydrazyl (DPPH•) tests, Sample 1 (fermented) exhibited the highest antioxidant activity. Sample 3 (cultivated) was richer in total phenol–total tannin content. Sample 6 exhibited the highest α-glucosidase inhibition among antidiabetic activities, reaching 28.93%. Sample 5 capsules demonstrated the highest α-amylase inhibition at 51.50%. Sample 7 exhibited the most notable inhibition against both acetylcholinesterase (22.92%) and butyrylcholinesterase (13.37%). Samples 3, 6, and 8 were found to be more effective against Candida tropicalis with minimum inhibitory concentration (MIC) = 625 μg/mL. A comprehensive study on garlic products, including popular items from Turkish pharmacies and those grown under standard conditions, revealed diverse chemical compositions and multifaceted health properties.
- MakaleAçık Erişim2024Interactions of novel 1,3-diaryltriazene-sulfamethazines with carbonic anhydrases: Kinetic studies and in silico simulations
Lolak, Nabih; Türkeş, Cüneyt; Akocak, Suleyman; Duran, Hatice Esra; Işık, Mesut; Durgun, Mustafa; Beydemir, Şükrü
Sulfonamides, recognized as carbonic anhydrase (CA, EC 4.2.1.1) inhibitors, are crucial in treating diverse diseases, including epilepsy, glaucoma, bacterial infections, and various pathological processes, e.g., high blood pressure, rheumatoid arthritis, ulcerative colitis, pain, and inflammation. Additionally, therapeutically, 1,3-diaryl-substituted triazenes and sulphamethazines (SM) are integral components in various drug structures, and the synthesis of novel compounds within these two categories holds substantial significance. Herein, ten 1,3-diaryltriazene-substituted sulphamethazine derivatives SM(1–10), which were created by reacting the diazonium salt of sulphamethazine with substituted aromatic amines, were synthesized and the physiologically and pharmacologically relevant human (h) isoforms hCA I and II, cytosolic isozymes, were included in the study. The synthesized compounds showed excellent inhibition versus hCAs; the 4-butoxy (SM7, KI of 5.69 ± 0.59 nM) compound exhibited a potent inhibitory effect against the hCA I compared with the reference drug acetazolamide (AAZ, KI of 116.00 ± 8.48 nM). The 4-cyano (SM4, KI of 5.87 ± 0.57 nM) compound displayed higher potency than AAZ (KI of 57.25 ± 4.15 nM) towards hCA II. Meanwhile, among the synthesized molecules, the 3,4-dimethoxy (SM9, KI of 74.98 ± 10.49 nM, SI of 9.94) compound (over hCA I) displayed a noticeable selectivity for hCA isoform II. The target compounds in the molecular docking investigation were determined to take part in various hydrophilic and hydrophobic interactions with nearby amino acids and fit nicely into the active sites of the hCAs. This research has yielded compounds displaying varying affinity toward hCA isoenzymes, ultimately serving as potent and selective hCA inhibitors. Given its substantial biological inhibitory potency, this particular derivative series is determined to hold the potential to serve as a promising lead compound against these hCAs.
- MakaleYalnız künye2024Bioactivity, cytotoxicity, and molecular modeling studies of novel sulfonamides as dual inhibitors of carbonic anhydrases and acetylcholinesterase
Güleç, Özcan; Türkeş, Cüneyt; Arslan, Mustafa; Demir, Yeliz; Dincer, Busra; Ece, Abdulilah; Küfrevioğlu, Ömer İrfan; Beydemir, Şükrü
In the present medical epoch, the prevailing approach of drug discovery, which focuses on inhibiting a single target, has been superseded by the concept of designing drugs that target more than one specific site. Herein, we present the design and synthesis of a novel series of 1-oxo-3,4-dihydronaphthalene substituted sulfonamides (5a-j) with human carbonic anhydrase (hCA) and acetylcholinesterase (AChE) inhibitory activities as potential dual-target agents. The design of target compounds 5a-j incorporated two key elements: i) The sulfonamide group, which is widely recognized for its high affinity for the active site of hCAs, and ii) the effectiveness of 2-benzylidene-1-tetralone class compounds in some neurodegenerative disorders. All reported sulfonamides were evaluated for their inhibitory effect against hCA I, II isoforms, and AChE. Both hCAs, and AChE were potently inhibited by 5a-j with KI constants in the nanomolar range; 47.71–113.20 nM, 28.05–206.60 nM, and 87.38–136.60 nM, respectively, compared to standard inhibitors, acetazolamide (KIs of 439.17 and 98.28 nM for hCA I and hCA II, respectively) and tacrine (KI of 159.61 nM). 5i exhibited the best multi-target inhibitory activity against hCA I (KI of 47.71 nM), hCA II (KI of 30.75 nM), and AChE (KI of 88.97 nM), while 5b showed a selectivity index (hCA I/II) of 3.61. Moreover, a cytotoxic activity assay for sulfonamides 5a-j was applied on the human breast adenocarcinoma MCF-7 cell line as well as on the non-tumoral mouse fibroblast L929 cell. Thereafter, extensive molecular dynamics simulations revealed the dynamic behavior of ligand/enzyme complex and predicted vital ligand contacts in the binding pocket.
- MakaleAçık Erişim2024The correlation between children’s health literacy level and healthy eating self-efficacy
Kasimoğlu, Necla; Gürarslan Baş, Nazan
Background The school environment provides an important opportunity for children and educators to develop health education and health literacy the ability to make rational health decisions and identify and determine factors that affect health. The aim of this study is to determine the correlation between health literacy and eating self-efficacy levels of primary school students and the affecting factors. Methods The study was conducted with 486 fourth graders studying in a primary school located in eastern Turkey between September 2021 and June 2022. The data were collected through face-to-face interviews using the Personal Information Form, the Health Literacy for School-Age Children Scale (HLSAC), and the Healthy Eating Self-Efficacy Scale for Children (HESES-C). Data were analyzed by number, percentage, mean, independent samples t-test, non-parametric Mann–Whitney U and Kruskal–Wallis tests, and Pearson’s correlation tests. Results The gender of the students and the education level of their mothers had a statistically significant correlation with their mean HESES-C and HLSAC scores scores (p<0.05). There was a significant negative correlation between the students’ HLSAC and their HESES-C mean scores. Conclusions According to the findings of the study, the students had a moderate level of health literacy and a low level of healthy eating self-efficacy. There was a negative relationship between health literacy levels and healthy eating self-efficacy.
- MakaleAçık Erişim2024Harnessing the power of social media: transforming nursing education for unmatched academic success
Erden, Yasemin; Aydın Kahraman, Handan; Çiftçi, Bahar
Aim: This study aimed to determine the effect of social media-supported learning on the academic achievement of nursing students and their social media usage levels in nursing education. Background: Using social media in nursing education enables students to experience interactive learning, strengthening learning outcomes and creating an enriched learning environment with up-to-date information. Method: The study was conducted as a randomized controlled study. CONSORTchecklist was completed. The subject of parenteral drug administration was explained interactively to all students by the same instructors for a total of 12 h for weeks. After the theoretical course, each skill was demonstrated by the instructor in the laboratory setting in a 24-h laboratory course with the demonstration method in line with the checklists. After the training was completed, the students filled out the necessary forms. Videos and informative images about parenteral drug applications were shared with the intervention group for 4 weeks through an Instagram page created for social media-supported learning. Questions and answers were shared on the subject every day in the story section. The answer to the question was shared in the story section the next day with explanations. At the end of the 4th training week, the students were asked to complete the necessary forms as a post-test. Changes in academic achievement and social media usage after the intervention were measured using achievement tests and the Social Media Scale in Nursing Education. Data were analyzed with SPSS 23.0. Normality was tested using the Shapiro–Wilk test. Independent t-tests and the Chi-Square test for categorical variables were used for normally distributed continuous variables. Pre-test and post-test comparisons within groups were evaluated with the Paired t-test if normality was met, or the Wilcoxon test. The effect size was calculated using Cohen’s d. Results: After social media-supported learning, it was found that the difference between the post-test mean scores of the nursing students in the intervention and control group was statistically significant(p < 0.001). It was also found that the average of the exam results after the training increased compared to the average before the training, and the difference between the two scores was statistically significant(p < 0.001). Conclusion: Social media-supported education positively affected the academic achievements of nursing students and the level of social media use in nursing education.
- MakaleYalnız künye2024Hepatoprotective effects of resveratrol on α-amanitin-induced liver toxicity in rats
Gezer, Arzu; Ustundag, Hilal; Mendil, Ali Sefa; Bedir, Gursel; Duysak, Lale
Objective: The hepatoprotective effects of resveratrol against α-Amanitin (α-AMA)-induced liver toxicity were investigated in an experimental rat model, focusing on oxidative stress, inflammation, apoptosis, and liver function. Methods: Thirty-two male Sprague-Dawley rats were divided into four groups (n = 8 per group): Control, resveratrol, α-AMA, and resveratrol+α-AMA. The resveratrol group received 20 mg/kg resveratrol orally for 7 days. The α-AMA group received 3 mg/kg α-AMA intraperitoneally on the 8th day. The resveratrol+α-AMA group received 20 mg/kg resveratrol orally (7 days) followed by 3 mg/kg α-AMA intraperitoneally on the 8th day. Liver tissues and blood samples were collected 48 h after α-amanitin administration for histopathological, immunohistochemical (NFkB, LC3B), and biochemical analyses (GSH, MDA, CAT, GPx, MPO, NOS, AST, ALT). Results: α-AMA significantly increased AST and ALT levels, oxidative stress marker (MDA), and inflammatory marker (MPO), while reducing antioxidant levels (GSH, CAT, GPx) and NOS concentration (P < 0.001 for all parameters). Histopathological analysis showed severe liver damage with increased NFkB and LC3B expression. resveratrol treatment significantly reduced AST and ALT levels (P < 0.01 for both parameters), decreased MDA and MPO levels, and increased NOS concentration, GSH, CAT, and GPx levels (P < 0.05 for all parameters). Reduced NFkB and LC3B expression in the resveratrol+α-AMA group and showed histopathological improvements. Conclusion: Resveratrol demonstrated substantial hepatoprotective effects against α-AMA induced liver toxicity by reducing oxidative stress, inflammation, and apoptosis, and improving liver function. These findings suggest that resveratrol could be a potential therapeutic agent for treating liver damage caused by potent hepatotoxins like α-AMA.
- MakaleAçık Erişim2024Invasive Aspergillosis with Cavernous Sinus Thrombosis Following High-Dose Corticosteroid Therapy: A Challenging Case of Rhino-Orbital-Cerebral Mycosis
Karakeçili, Faruk; Barkay, Orçun; Sümer, Betül; Binay, Umut Devrim; Memiş, Kemal Buğra; Yapıcıer, Özlem; Balcı, Mecdi Gürhan
Invasive aspergillosis is a rare but severe fungal infection primarily affecting immunocompromised individuals. The Coronavirus Disease-2019 (COVID-19) pandemic has introduced new complexities in managing aspergillosis due to the widespread use of corticosteroids for treating COVID-19-related respiratory distress, which can increase susceptibility to fungal infections. Here, we present a challenging case of progressive cerebral aspergillosis complicated by cavernous sinus thrombosis (CST) in a 67-year-old male with a history of COVID-19. The patient, initially misdiagnosed with temporal arteritis, received pulse corticosteroid therapy twice before presenting with persistent left-sided headaches and vision loss. Cranial imaging revealed findings consistent with fungal sinusitis, Tolosa–Hunt syndrome, and orbital pseudotumor, which progressed despite initial antifungal therapy. Subsequent magnetic resonance imaging indicated an invasive mass extending into the left cavernous sinus and other intracranial structures, raising suspicion of aspergillosis. A transsphenoidal biopsy confirmed Aspergillus infection, leading to voriconazole therapy. Despite aggressive treatment, follow-up imaging revealed significant progression, with extension to the right frontal region and left cavernous sinus. The patient then developed visual impairment in the right eye and was diagnosed with CST secondary to fungal sinusitis. Management included a combination of systemic antifungals and antibiotics; however, the patient declined surgical intervention. This case underscores the diagnostic challenges and rapid progression associated with cerebral aspergillosis in post-COVID-19 patients treated with corticosteroids. This report highlights the need for heightened clinical suspicion and prompt, targeted interventions in similar cases to improve patient outcomes. Further research is required to understand the optimal management of invasive fungal infections.
- MakaleAçık Erişim2024Associations between the Framingham Risk Score and cognitive functions in people with multiple sclerosis
Ersoy, Alevtina; Baloglu, Senanur; Tanoglu, Ceyda; Hayme, Serhat
Background & Objective: Cognitive impairment is common in people with multiple sclerosis (MS). The Framingham Risk Score (FRS) is an algorithm used to estimate the 10-year risk of developing cardiovascular disease (CVDr). This study aimed to evaluate the possible associations between FRS and cognitive impairment in people with MS. Methods: Demographic characteristics, laboratory parameters, clinical findings, vascular risk factors, and Brief International Cognitive Assessment for MS (BICAMS) test results, FRS and CVDr scores were recorded for 110 MS patients. Results: FRS and CVDr scores exhibited a significant negative correlation with the number of correct responses in the Symbol Digit Modalities Test (p < 0.001 for all) and California Verbal Learning Test–Second UK Edition (CVLT-II) test (p < 0.001 for all), and significant positive correlation with the number of incorrect responses in the CVLT-II (p < 0.001 for all). The total score of the Brief Visuospatial Memory Test Revised had a negative correlation with FRS and CVDr (p < 0.001 for both). BICAMS scores showed no significant correlation with smoking status, homocysteine, and uric acid levels. Conclusion: We suppose that vascular risk factors and FRS and CVDr scores may be associated with the deterioration of working memory, information processing speed, verbal learning, and memory in people with MS. Based on the potential impact of vascular risk factors on cognitive functions, our findings suggest that lifestyle changes, appropriate treatment, and using a multidisciplinary approach toward vascular risk factors during MS management may exhibit a positive effect on cognition in people with MS.
- MakaleYalnız künye2024The efficacy of interventions designed for parents of children with developmental disabilities in Türkiye: A systematic review and meta-analysis
Erbiçer, Eyüp Sabır; Koç, Muharrem; Haskan Avcı, Özlem; Ağırkan, Murat; Şen, Sedat; Akın, Ercan; Metin, Ahmet; Erbiçer, Sinem
Previous studies have revealed that parents of children with developmental disabilities (DD) experience various mental health problems. The number of intervention studies has increased for parents of children with DD to reduce symptoms of mental health conditions and increase positive functioning in recent years in Türkiye. The main purpose of this systematic and meta-analytical review is to address the efficacy of interventions for parents of children with DD on symptoms of mental health conditions and positive functioning in Türkiye. The second purpose of this study is to examine the effect of the potential moderators on the efficacy of the interventions in symptoms of mental health conditions and positive functioning. For these purposes, studies published between 2000 and October 2022 were searched in various databases. As a result of the literature search, 61 studies met the inclusion criteria, and 23 studies with 17 independent effect sizes for symptoms of mental health conditions and 12 independent effect sizes for positive functioning were included in the current study. Cohen's d effect size index was used to calculate the efficacy of intervention studies included in the meta-analysis on symptoms of mental health conditions and positive functioning. Results revealed that the overall mean effect size for the 17 mental health studies was −1.15 and for the 12 positive functioning studies was 2.03 in favor of experimental group. Moderator analyses showed that the study design (experimental vs. quasi-experimental) was a statistically significant moderator of positive functioning. The average effect size for experimental studies was higher than that of quasi-experimental studies. However, other moderators (the gender of the participants, publication type, the type of intervention, the disability type of children, the sample size, and the number of sessions) were not statistically significant for symptoms of mental health conditions and positive functioning. Although the current study has some limitations, the findings revealed that the interventions designed for parents of children with DD result in an increase in positive functioning and a reduction in symptoms of mental health conditions in favor of experimental group.
- DüzeltmeAçık Erişim2024Correction to: Comparison of Selenic Acid and Pyruvic Acid-Loaded Silver Nanocarriers Impact on Colorectal Cancer Viability (Journal of Fluorescence, (2024), 34, 3, (1025-1037), 10.1007/s10895-023-03339-4)
Erdemir, Gökçe; DANIŞMAN-KALINDEMIRTAŞ, Ferdane; KARIPER, İshak Afşin; KURUCA, Dürdane Serap; ÖZERKAN, Dilşad
The original version of this article should be updated. One of the author of this article ‘Gökçe Erdemir’ want to add his another affiliation. The author’s affiliations are now updated. The original article has been corrected.
- MakaleYalnız künye2024Effect of taxifolin on clozapine-induced experimental oxidative and inflammatory heart damage in rats; [Effet de la taxifoline sur les lésions cardiaques oxydatives et inflammatoires expérimentales induites par la clozapine chez le rat]
Akyuz Cim, Emine Fusun; Suleyman, Halis
Aim: Clozapine is an atypical antipsychotic (AAP) drug used in treatment-resistant schizophrenia patients. The adverse effects of clozapine on the heart may result in death and require drug discontinuation. Inflammatory mechanisms are thought to be responsible for the negative effect of clozapine on the heart. This suggests that using an agent with anti-inflammatory and antioxidant properties, which may be specific to clozapine-induced heart damage, may prevent possible damage. The aim of our study is to evaluate the effect of taxifolin (3,5,7,3’,4’-pentahydroxy flavanone), an agent with known antioxidant and anti-inflammatory effects, on myocardial damage caused by clozapine with biochemical and histopathological data. Material and method: The rats were divided into three equal groups: healthy control group (HC), clozapine-treated group (CLN), and taxifolin + clozapine-treated group (TCL). To perform this experiment, taxifolin was administered to TCL (n-6) rats at a dose of 50 mg/kg orally by gavage into the stomach. In the HC (n-6) and CLN (n-6) groups, the same volume of distilled water was administered orally as a solvent. One hour after the administration of taxifolin and distilled water, clozapine was administered orally at a dose of 20 mg/kg to the TCL and CLN groups once a day for 28 days. At the end of the period, troponin I (TP I) and creatine kinase MB (CK-MB) levels were measured in the venous blood of each group. Malondialdehyde (MDA), total glutathione (tGSH), TNF-α, NF-[Formula presented]B, and IL-1β levels were measured in samples taken from heart tissues. Additionally, heart tissues were evaluated histopathologically. Results: Troponin I, CK-MB, MDA, TNF-α, NF-[Formula presented]B, and IL-1β levels, myocardial degeneration, myofiber irregularity, and congestion scores were significantly higher and tGSH levels were lower in the clozapine group than in the healthy control and taxifolin + clozapine groups (P < 0.05). Compared with the healthy control group, troponin I, tGSH, and NF-KB levels were similar (P > 0.05), CK-MB, MDA, TNF-α, and IL-1β levels were higher in the taxifolin + clozapine group, while they were significantly lower than the clozapine group (P < 0.05). Histopathologically evaluated myocardial degeneration, myofiber irregularity, and congestion score were significantly lower in the taxifolin + clozapine group than in the clozapine group. In the clozapine group (CLN group), myofibers were found to have irregular patterns and were observed as irregular. In the taxifolin + clozapine group (TLC group), myofibrils generally showed a regular morphology. Conclusion: We found that taxifolin can ameliorate damage to myocardial tissue by regulating oxidant-antioxidant and proinflammatory cytokine levels. The data of our study suggest that taxifolin may be useful in the treatment of clozapine-induced myocardial injury.
- MakaleYalnız künye2024Docosahexaenoic acid eliminates endoplasmic reticulum stress and inflammatory pathways in diabetic rat keratopathy
Gezer, Arzu; Özkaraca, Mustafa; Üstündağ, Hilal; Soydan, Menekşe; Alkanoğlu, Ömer; Bedir, Gürsel
Diabetic keratopathy, characterized by corneal structural changes, is a common complication of diabetes mellitus (DM). Docosahexaenoic acid (DHA), an omega-3 fatty acid, has shown potential therapeutic benefits in various diabetic complications. This study aimed to investigate the protective effect of DHA on corneal tissue in streptozotocin (STZ)-induced type 2 DM in rats. Forty male Sprague–Dawley rats were randomly assigned to four groups (n = 10 per group): Control, DHA, DM, and DM + DHA. The DHA group received DHA by oral gavage at a dose of 100 mg/kg daily for 10 days. In the DM group, diabetes was induced by a single intraperitoneal injection of STZ at 50 mg/kg. Confirmation of diabetes induction was based on monitoring fasting blood glucose levels on the third day post-injection. The DM + DHA group underwent the same diabetes induction protocol with STZ and received DHA at 100 mg/kg daily via oral gavage for 10 consecutive days. Corneal tissue samples were collected at the end of the study period for histopathological, immunohistochemical, qRT-PCR, and ELISA analyses. Histopathological analysis showed significant edema, angiogenesis, and degeneration in the DM group compared to the control (p < 0.001). DHA treatment significantly mitigated these changes, approaching control levels (p < 0.01). Immunohistochemistry showed increased VEGFR2 and iNOS expression in the DM group, which was significantly reduced in the DM + DHA group (p < 0.01). qRT-PCR results indicated a significant decrease in Bcl-2 expression (p < 0.001) and an increase in ATF-6, IRE1, NF-κB, TNF-α, IL-1β, NLRP3, Bax, and Caspase-3 expressions in the DM group (p < 0.001). ELISA analyses revealed significantly elevated levels of inflammatory markers NF-κB, TNF-α, IL-1β, and IL-6 in the DM group compared to the control (p < 0.001). DHA treatment significantly upregulated Bcl-2 and downregulated apoptotic and inflammatory markers (p < 0.01). DHA demonstrated significant protective effects against STZ-induced corneal damage in diabetic rats by modulating apoptotic and inflammatory pathways. These findings suggest that DHA may be a promising therapeutic agent for preventing diabetic keratopathy.
- MakaleYalnız künye2024Evaluation of α-glucosidase inhibitor activity and bioactive compounds in purple wheat flour yogurts
Çebi, Kadir; Yangılar, Filiz
Nowadays, the food industry attaches more importance to studies on using purple components in food formulations due to their bioactive properties. This study evaluated the phenolic compound content, antioxidant activity, physicochemical properties, sensory properties, and α-glucosidase activities in yogurt enriched with purple wheat flour. Yogurts were produced using varying concentrations of purple wheat flour labeled as sample A (1.5 %), sample B (3 %), and sample C (4.5 %). It was observed that incorporating purple wheat flour led to a decrease in pH and an increase in L*, a*, and b* values, as well as the acidity and viscosity of the yogurts. Sample C contained the highest phenolic content (37.6 mg GAE/100 g dry matter) on day 14, along with the highest flavonoid content (14.59 mg CE/100 g) on day 21 when than control yogurt. In addition, sample C had the highest α-glucosidase activity (38.35 %) on day 14 and anthocyanin content (13.55 g/100 g) on day 21. As a result, C yogurt can be consumed as a diabetic product with antidiabetic and antihypertensive properties by reaching optimal α-glycosidase inhibition activity. Furthermore, yogurts containing purple wheat flour received higher sensory scores from panelists.
- MakaleAçık Erişim2024Assessment of the Antioxidant, Antiproliferative and Antityrosinase Potential of Unripe Fruit of Prunus x domestica L.
Güven, Zühal Bayrakçeken; Yuce, Neslihan; Basaran, A. Ahmet
Prunus x domestica L. is a valuable plant that belongs to the Rosaceae family and is distributed worldwide. The aim of this study is to investigate the biological effect of the unripe green fruits of P. domestica, which have only been studied to a limited extent so far. The antioxidant capacity of the fruit extracts and subfractions was investigated by DPPH, TEAC and CUPRAC assays. The antiproliferative effect was investigated against the cell lines L929, CaCo-2 and PC-3. While the highest antiproliferative effect against cancer cell lines was found in the unripe fruit ethyl acetate subfraction, extracts and fractions showed no cytotoxic effect on the healthy cell line L929. The fruit ethyl acetate fraction showed a strong inhibition of the enzyme tyrosinase with an IC50: 51.83 μg/ mL compared to standard compound kojic acid (IC50: 21 μg/mL). It was found that the methanol extract from unripe fruits as well as the ethyl acetate and aqueous subfractions exhibited strong antioxidant activity, showed concentration-dependent cytotoxic activity on PC-3 and CaCo-2 cells, but had no cytotoxic effect on healthy L929 cells. The high antioxidant capacity of the fruits, their selective cytotoxic effect on prostate and colon cancer cells and their strong tyrosinase inhibitory activity suggest that they could be a new, safe and cost-effective source for the pharmaceutical and cosmeceutical industries.
- MakaleYalnız künye2024The effect of caregivers’ health literacy levels on the quality of life and self-care of patients with heart failure
Selvi Sarıgül, Seval; Ürek, Duygu; Uğurluoğlu, Özgür
Background: The aim of this study is to examine the effects of health literacy levels of caregivers on the self-care behaviors and quality of life of patients aged 65 years and older with heart failure. Methods: The study was conducted in a university hospital in Erzincan, Türkiye between November 4, 2023, and February 12, 2024, on patients hospitalized in internal medicine, cardiology, and cardiovascular surgery wards and their caregivers (n = 206 pairs). Results: As a result of the multivariate regression analysis, it was determined that the access, appraisal, and application sub-dimensions of the health literacy of the caregivers positively affected the self-care behaviors and quality of life levels of the patients. Conclusions: These results reveal the significant effect of the health literacy levels of the caregivers in the management process of heart failure disease. Healthcare providers -especially nurses- can improve patient outcomes of patients with heart failure by considering the health literacy level of their caregivers and using innovative educational methods that can improve the health knowledge and skills of caregivers.
- MakaleAçık Erişim2024The Efficacy of Glecaprevir/Pibrentasvir in Chronic Hepatitis C Patients and the Impact of the COVID-19 Pandemic: Multicenter Real-Life Data
Gürbüz, Yunus; Kocagül-çelikbaş, Aysel; Öztoprak, Nefise; Aygen, Bilgehan; Batırel, Ayşe; Habiloğlu, Arif Doğan; Aktuğ-Demir, Nazlım; Çeken, Sabahat; Demirtürk, Neşe; Ceylan, Mehmet Reşat
Objective: This study aimed to demonstrate the real-life efficacy and safety of glecaprevir / pibrentasvir in the treatment of chronic hepatitis C, as well as to identify the problems caused by the COVID-19 pandemic in the follow-up and treatment of patients. Materials and Methods: The study was conducted retrospectively with the participation of researchers from universities or training and research hospitals. It included patients with chronic hepatitis C who were over 18 years of age, treatment-naïve or treatment-experienced, had detectable HCV RNA and were receiving glecaprevir/pibrentasvir treatment Results: Only 188 of the 385 patients who participated in the study came to the follow-up visit 12 weeks after treatment, and all of them had a sustained virological response. It was thought that a significant portion of the 177 patients who did not come to the follow-up visit at 12 weeks after treatment refrained from coming to the hospital due to the COVID-19 pandemic. None of the patients who attended the follow-up visits required treatment discontinuation due to adverse events. Conclusion: Glecaprevir/pibrentasvir is a highly effective and relatively safe drug in the treatment of chronic hepatitis C. The COVID-19 pandemic has negatively affected the follow-up and treatment processes of patients. New measures are needed for the follow-up and treatment of patients with chronic hepatitis C during pandemics.
- Derleme MakaleAçık Erişim2024Wastes in Automotive Maintenance Businesses, Its Effects on Employees and Precautions
Çetin, Murat; Demirci, Oğuz Kürşat
This study offers an exhaustive analysis of the emissions and waste produced by vehicle maintenance and repair activities, underscoring their detrimental impacts on the health and ergonomic conditions of service workers. It underscores the critical need for the implementation of straightforward yet effective measures to mitigate these adverse effects. The paper delineates a variety of strategies aimed at enhancing the well-being of maintenance personnel by minimizing their exposure to harmful substances and improving workplace ergonomics. Heightened environmental awareness has positively influenced research on waste disposal methods. Implementing environmentally friendly waste and emission disposal methods in automotive maintenance is projected to reduce costs for mechanics and operators. Furthermore, the study elaborated on the comprehensive processes undertaken in automotive maintenance facilities, detailing the journey of waste from its origin to its final disposal. Effective management of these processes provides significant environmental and economic benefits. Additionally, the study presents a series of evidence-based recommendations designed to cultivate a safer and healthier working environment for automotive service professionals. These recommendations aim to improve overall working conditions in the industry by contributing to the enhancement of occupational health and safety standards. Through these measures, the research advocates for a comprehensive approach to occupational health within the automotive repair industry.
