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- Suleyman, Halis(167)
- Mammadov, Renad(95)
- Türkeş, Cüneyt(84)
- Aydin, Sonay(83)
- Altuner, Durdu(74)
- Suleyman, Bahadir(68)
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- Arslan, Yusuf Kemal(50)
- Sofu, Hakan(50)
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- Karakeçili, Faruk(47)
- Altay, Ahmet(46)
- Huyut, Mehmet Tahir(46)
- Aydin, Suleyman(45)
- Türkmenoğlu, Burçin(45)
- Kuzucu, Mehmet(40)
- Yazici, Gulce Naz(40)
- Peker, Kemal(39)
- Algul, Oztekin(38)
- Cicek, Betul(37)
- Mertoglu, Cuma(36)
- Camurcu, Yalkin(35)
- Suleyman, Zeynep(34)
- Bozkurt, Aliseydi(32)
- Mendil, Ali Sefa(32)
- Gunay, Murat(31)
- Gözcü, Sefa(31)
- Köksal, Ekrem(31)
- Yuca, Hafize(31)
- Dilek, Esra(30)
- Karakurt, Papatya(30)
- Ozcicek, Fatih(30)
- Süleyman, Halis(30)
- Onk, Didem(29)
- Ucpunar, Hanifi(29)
Yayınlar
- ArticleAçık Erişim2026Role of ureteral wall thickness and computed tomography imaging in predicting spontaneous passage of ureteral stones
Kadırhan, Özlem; Aydın, Sonay; Keskin, Ercüment; Kantarcı, Mecit
PURPOSE Urolithiasis is a common health problem with a high recurrence rate, and effectively balancing follow-up with intervention is important for patient safety. In this context, our study aims to identify criteria that can predict the likelihood of spontaneous passage (SP) of ureteral stones. METHODS A retrospective analysis was performed on 2,773 patients who presented to our hospital with renal colic over a 4-year period. The study included 897 patients with unilateral ureteral stones measuring ≤10 mm, identified using non-contrast computed tomography, and inflammatory serum markers assessed through biochemical testing. Variables analyzed to predict the likelihood of SP included stone size, lateralization and location, ureteral wall thickness (UWT) at the stone site, stone density, degree of hydronephrosis (HN), ureteral length, parenchymal thickness and density, and various biochemical parameters. RESULTS It was determined that the SP of ureteral stones was considerably affected by larger stone size (right >6.5 mm, left >6 mm), higher stone density (>957 Hounsfield units), increased UWT (>1.7 mm), presence of high-grade HN (grade ≥2), and elevated neutrophil-lymphocyte ratio (NLR) (>2.15) and platelet-lymphocyte ratio (PLR) (>10.28) values in blood. No statistically significant relationship was observed between SP and ureteral length, renal parenchymal thickness, or renal parenchymal density. It was found that when the UWT at the level of the ureteral stone exceeded 1.7 mm, the risk of the stone not passing spontaneously increased by 706.5 times in univariate logistic regression (LR) analysis and by 337.9 times in multivariate LR analysis compared with individuals with a wall thickness below this threshold. CONCLUSION Our study demonstrated that, in addition to stone size and location, increased UWT at the stone level, higher stone density, the presence of concomitant high-grade HN, and elevated NLR and PLR values in the blood could be used as criteria to determine the likelihood of SP of ureteral stones. According to our results, UWT was shown to be a stronger risk factor for failure of SP than stone size. CLINICAL SIGNIFICANCE The findings indicate that wall thickness around ureteral stones is a risk factor with a higher negative predictive value for SP than the stone size and location. Copyright @ 2026 Author(s)
- ArticleAçık Erişim2026Association of mandibular third molar positions, ramus height, and gonial angle on panoramic radiographs: a retrospective cross-sectional study
Altındağ, Ali; Akyüz, Mehmet; Uzun, Sultan; Yüksel, İbrahim Burak; Mağat, Güldane
Background: The aim of this study was to investigate the relationship between the positions of impacted mandibular third molars and the gonial angle and ramus height, as measured on panoramic radiographs, according to the Pell and Gregory/Winter classifications. Methods: This retrospective study included panoramic radiographs of 700 patients (350 males and 350 females) aged 18–62 years, who presented with bilaterally impacted mandibular third molars. The gonial angle and ramus height were measured, and the position of the impacted third molars was categorized based on the Pell and Gregory/Winter classification systems. Results: The most common third molar impaction position observed was vertical, followed by mesioangular and horizontal positions. The study found that a high gonial angle was more frequently observed in males, while a low gonial angle was more common in females. No significant association was identified between the type of mandibular third molar impaction and the gonial angle or ramus height. Additionally, individuals with high gonial angles had lower ramus heights. Individuals with impacted teeth in the “other” position had statistically higher gonial angles (p < 0.05). Conclusion: The study concludes that there is no significant correlation between the gonial angle, ramus height, and the type of mandibular third molar impaction. However, individuals with impacted teeth in the “other” position were found to have higher gonial angles, which may increase the risk of mandibular fractures. These findings show that although certain impaction types are more prevalent, mandibular anatomical parameters such as gonial angle and ramus height do not appear to significantly influence impaction patterns when assessed on panoramic radiographs.
- ArticleAçık Erişim2026Embryologic layers in dermatology: Developmental checkpoint disorders, diagnostic insight, and regenerative futures
KAYA, GÖKHAN; YAZICI, GÜLCE NAZ; ALAVANDA, CEREN; KÜÇÜK, ÖZLEM SU
Objectives: This review aims to present a developmental framework linking embryonic lineage with non-heritable cutaneous anomalies to improve diagnostic precision and educational approaches in dermatology. Materials and Methods: A narrative literature review was conducted using PubMed, Scopus, and Web of Science databases covering the years 2000-2025. Keywords included “skin development” “embryology,” “developmental checkpoint disorders,” and “non-genetic congenital disorders.” Data on morphogenesis, embryologic signaling pathways, and representative disorders were synthesized into a layer-based model. Results: Disorders such as selfhealing collodion baby (periderm retention anomaly), pigmentary mosaicism (postzy-gotic melanocyte patterning defect), and focal dermal hypoplasia (connective tissue maldevelopment) reflect disruptions at specific morphogenetic checkpoints. Mapping these conditions to their embryonic origins revealed layer-specific vulnerability windows and facilitated differential diagnosis from inherited disorders. Understanding these embryologic principles supports earlier diagnosis, informed prenatal counseling, and structured integration into dermatology curricula. Advances in regenerative medicine, particularly stem cell-based strategies, highlight the translational potential of dermatoembryology in developing targeted therapies. Conclusion: A layer-oriented der-matoembryological perspective enhances recognition of developmental skin disorders, especially when genetic analyses are inconclusive. Incorporating embryologic concepts into clinical reasoning not only improves diagnostic accuracy but also fosters regenerative therapeutic innovations and enriches dermatology education.
- ArticleAçık Erişim2026Mothers’ experiences of journaling during their infants’ stay in a baby-friendly NICU: a hermeneutic phenomenological study
Kasımoğlu, Necla; Gürol, Ayşe; Metin, Ayşe; Polat, Şevin
Background: The experience of mothers keeping a diary during their baby’s stay in the Neonatal Intensive Care Unit (NICU) can be deeply significant, serving as a coping mechanism and a means of emotional expression. This study aimed to explore mothers’ experiences during their infants’ hospitalization in the NICU, as reflected in maternal diary entries, using a hermeneutic-phenomenological approach. Methods: A hermeneutic phenomenological approach was employed to gain an in-depth understanding of mothers’ experiences in the NICU. The participating mothers were asked to complete a one-page diary entry for five consecutive days, documenting their perceptions of emotional closeness and the time spent with their infants. Results: The findings revealed that mothers initially experienced emotional detachment and uncertainty due to physical separation from their infants. However, over time, as they engaged in skin-to-skin contact, participated in caregiving activities, and adapted to the NICU routine, their sense of emotional closeness gradually increased. The journaling process provided a structured means for mothers to reflect on their emotions, track their bonding experiences, and process the challenges associated with NICU hospitalization. Additionally, factors such as nursing support, opportunities for parental involvement in infant care, and peer interactions played a significant role in shaping emotional closeness. Conclusion: The study highlights the importance of structured interventions that promote emotional closeness between mothers and their infants in the NICU. Encouraging early parental involvement, facilitating skin-to-skin contact, and incorporating expressive writing techniques such as journaling into nursing practices may help strengthen mother-infant bonding. Future research should focus on evaluating the long-term effects of these interventions on maternal well-being and infant development.
- ArticleAçık Erişim2026Evaluation of Osteonecrosis Risk Using Serum C-Terminal Cross-Linked Telopeptide of Type 1 Collagen (CTX-1) Levels in Osteoporotic Patients: Effects of Drug Holidays and Risk Factors
Ergül, Emine Esra; Laçin, Oğuzhan; Kılıçaslan, Hamide Özge; Bostancı, Fahrettin; Özdemir, Elif Can; Kılıç, Ayşegül; Hayme, Serhat; Ayçiçek, Hilal Büşra
Background: The aim of this study is to evaluate the risk of osteonecrosis of the jaw in patients receiving osteoporosis treatment using C-terminal cross-linked telopeptide of type 1 collagen (CTX-1) values, to analyze follow-ups after drug holidays, and to compare treatment agents and other risk factors to determine the association with CTX-1 results. Methods: A total of 273 patients (266 female and 7 male) who received bisphosphonate and denosumab treatment for osteoporosis were included in this retrospective study. Sociodemographic characteristics, vitamin D levels, serum CTX-1 level, presence of diseases affecting CTX-1 levels, type of bisphosphonate, duration of use, presence of drug holidays, and duration of denosumab use (if any) were recorded. The effects of bisphosphonates and denosumab on CTX-1 levels were compared, and differences in the risk of osteonecrosis between them were evaluated. Results: In this study, a meaningful association was not identified between serum CTX-1 levels and the measured vitamin D values (P = .232). Patients who underwent a drug holiday had significantly higher mean serum CTX-1 levels (266.2 ± 175.1 pg/mL) compared to those without a drug holiday (199.9 ± 138.5 pg/ mL; P = .009). Higher CTX-1 levels were observed in individuals receiving ibandronate and alendronate, whereas the lowest values were detected in patients treated with denosumab. Conclusion: Serum CTX-1 levels appeared unaffected by vitamin D. Although the denosumab group exhibited the highest risk of osteonecrosis, the difference compared to zoledronic acid was not statistically significant. These results suggest caution during jaw-related procedures and consideration of drug holidays when necessary.
- ArticleAçık Erişim2026Students' Online Burnout, Fatigue Situations and Associated Factors: A Cross-Sectional Study
Karakurt, Papatya; Firat, Meryem; Memiş, Şeyda Yetginler
Aim: This study was planned to determine online burnout, fatigue states of students and the associated factors. Design: This study was designed as a cross-sectional type of research. The study is of a quantitative type. Methods: The universe of this study consisted of all 890 students studying in the Department of Nursing and Nutrition Dietetics at the Faculty of Health Sciences in the spring semester of the 2023–2024 academic year at a university in the east of Türkiye. The sample of the study consisted of 570 students studying in the 2nd, 3rd and 4th grades. The ‘Introductory Information Form’ was used to collect data, and the ‘Zoom Burnout and Fatigue Scale’ was used to determine the students' online burnout and fatigue status. Percentage, mean, standard deviation, t-test in independent groups, variance (ANOVA) and Pearson correlation analysis were used in the analysis of data. Results: The mean age of the students included in the study was determined to be 21.77 ± 2.01; 40.9% were sophomores. It was determined that the students received 2.86 ± 1.07 points from the total scale. The maximum score obtained from the scale is 5. It was determined that there was a significant difference between the mean scores of the scale and its sub-dimensions according to the difficulty of studying with distance education compared with studying with face-to-face education, difficulty in accessing information sources with distance education and difficulty in using information technologies (p < 0.05). Conclusions: As a result, students are showing average levels of the Zoom Burnout and Fatigue. In line with these findings, it can be suggested that when delivering theoretical courses via distance education, active learning methods should be used, students' access to course materials should be facilitated and their participation should be supported or encouraged. Relevance to Clinical Practice: Since nursing and dietetics are hands-on profession, practices must take place in a hospital setting and face-to-face. Online education should be used as a supplementary tool. Students should apply the online education they receive to their practice. Patient or Public Contribution: Participants provided data but were not involved in the study's design, outcome measures or conduct.
- LetterAçık Erişim2026Statins enhance trastuzumab deruxtecan efficacy: preclinical synergy in HER2-negative models and clinical benefit in HER2-positive metastatic breast cancer
Sahin, Taha Koray; Orhan, Cemal; Ozercan, Ibrahim Hanifi; Muglu, Harun; Er, Besir; Akyildiz, Arif; Tunbekici, Salih; Oruc, Ahmet; Aykan, Musa Baris; Koylu, Bahadir
Trastuzumab deruxtecan (T-DXd) has demonstrated significant efficacy in metastatic breast cancer (mBC) with varying HER2 expression. Statins have been shown to increase HER2 membrane localization and stability, potentially enhancing responses to HER2-targeted therapies. We investigated whether statins enhance T-DXd activity through a translational approach combining a preclinical HER2-negative model and a real-world HER2-positive mBC cohort. In the preclinical arm, 60 female Wistar albino rats were induced with mammary tumors using N-methyl-N-nitrosourea (MNU). Animals were randomized into five groups: Control, MNU-only, MNU + T-DXd, MNU+statin, and MNU + T-DXd+statin. In the clinical arm, 109 patients with HER2-positive mBC who received T-DXd were retrospectively analyzed. The combination of T-DXd and statin significantly reduced tumor volume compared to either monotherapy (p < 0.0001) in a preclinical rat model. HER2 protein levels were found to be elevated in the statin and combination groups, as demonstrated by both IHC and Western blot analyses. In a real-world cohort of 109 heavily pretreated patients with HER2-positive mBC receiving T-DXd, concomitant statin use was associated with significantly improved median progression-free survival (mPFS) and median overall survival (mOS). Our findings revealed that statin use significantly enhanced the efficacy of T-DXd in both a preclinical HER2-negative rat model and patients with HER2-positive mBC. Prospective clinical trials are warranted to validate these observations.
- LetterAçık Erişim2026Expanding the framework of non-technical skills in resuscitation: why family communication matters
Kantar, Yusuf
- ArticleYalnız künye2026The frequency, predictors, and treatment options of spondyloarthritis in primary Sjögren’s syndrome: a single-center experience
Karataş, Beliz
The aim of this study is to define the frequency and predictors of axial spondyloarthritis and treatment options in patients with Sjögren syndrome. In our study, the frequency of spondyloarthritis according to the International Association for the Assessment of Spondyloarthritis (ASAS) criteria was investigated in 84 patients who were diagnosed with primary Sjögren syndrome according to the 2016 ACR / EULAR (American College of Rheumatology/European Association of Rheumatology Associations) classification criteria in the Internal Medicine Rheumatology clinic of our hospital between November 2023 and January 2025. Patients’ age, gender, HLAB-27, anti-nuclear antibody (ANA), anti-SSA, anti-SSB, inflammatory back pain, presence of sacroiliitis on sacroiliac joint magnetic resonance imaging (MRI), medications used, and Sjögren syndrome complications were recorded retrospectively. A total of 94% of the patients were female and 6% were male. The median age was 51 years. Axial spondyloarthritis (axSpA) was detected in 27% of the patients. Among those who developed axial spondyloarthritis, 15 patients received nonsteroidal anti-inflammatory drugs (NSAIDs), 5 patients received sulfasalazine, 1 patient received methotrexate, 1 patient received TNF (tumor necrosis factor) inhibitor, and 1 patient received secukinumab treatment. Four of those who received sulfasalazine had anterior uveitis and 1 had enthesitis. The only variable found to be associated with the presence of axial spondyloarthritis in primary Sjögren syndrome was ANA (p value: 0.006). Spondyloarthritis is not uncommon in primary Sjögren syndrome. ANA has been found to be associated with the presence of axial spondyloarthritis in primary Sjögren syndrome and has the potential for further research.
- ArticleAçık Erişim2026Awareness, attitudes, and educational use of artificial intelligence among medical students: a large cross-sectional survey
Kara, Ali Veysel; Harmancı, Hatice; Yılmaz, Yusuf
Background: Artificial intelligence (AI) is increasingly influencing medical education and clinical practice. Understanding medical students’ awareness, attitudes, and usage patterns of AI is essential for guiding curriculum development and ensuring responsible integration into undergraduate medical training. Methods: A nationwide cross-sectional survey was conducted between November and December 2025 among undergraduate medical students in Türkiye. A structured questionnaire assessed demographic characteristics, AI awareness and frequency of use, educational and clinical applications, attitudes toward AI, ethical concerns, and future educational expectations. A composite AI Attitude Score was calculated using three Likert-scale items, and internal consistency was evaluated using Cronbach’s alpha. Descriptive and inferential statistical analyses were performed. Results: A total of 1,346 medical students were included. Overall, 81.1% reported awareness of AI applications in medicine. AI tools were predominantly used for educational purposes (73.3%), whereas clinical or simulation-based use was limited (11.6%). The mean composite AI Attitude Score was 3.41 ± 0.61, indicating moderately positive perceptions (Cronbach’s alpha = 0.71). Ethical concerns were reported by 67.4% of participants. A majority (79.1%) expressed interest in receiving formal AI-related education. No significant differences were observed between preclinical and clinical students in overall attitudes or educational demand; however, clinical students reported significantly greater use of AI in clinical contexts (p < 0.001). Conclusion: Medical students demonstrate high awareness and generally positive attitudes toward AI; however, clinical integration remains limited and ethical concerns are prevalent. These findings underscore the need for structured, ethically grounded AI education within undergraduate medical curricula. Findings should be interpreted with consideration of potential self-selection bias due to voluntary online participitation.
- ArticleAçık Erişim2026Investigation of the relationships between polypharmacy, medication adherence, and quality of life in elderly individuals
Yetim, Birol; Selvi Sarıgül, Seval; Uğurluoğlu, Özgür; Çelik, Yusuf
The main purpose of this study is to identify the key factors affecting polypharmacy and medication adherence in elderly patients and to determine the impact of polypharmacy and medication adherence on their quality of life. The research was conducted on 326 patients aged 65 and older who applied to a family health center in Erzincan, Turkiye, between January and February 2025. Multivariate logistic regression analyses were used to analyze the data. The analyses indicated that increasing age affected polypharmacy, medication adherence, and quality of life of the elderly. Moreover, the type of disease and the medication cascade were identified as the determinants of polypharmacy, while the duration of the disease was observed to affect medication adherence. It was determined that polypharmacy affected medication adherence and quality of life, while gender and medication adherence also had a significant impact on the patient’s quality of life (p<0.05). These findings suggest that polypharmacy and medication adherence have significant effects on the quality of life of elderly individuals. Therefore, it is important to monitor and evaluate polypharmacy regimens periodically with evidence-based therapeutic strategies and well-structured strategic plans to improve patients’ quality of life and medication adherence.
- ArticleAçık Erişim2026The effect of povidone-iodine-enhanced rectal enema on procedure-related infectious complications before transrectal ultrasound-guided prostate biopsy: A prospective randomized study
Doğan, Mücahit; Aydin, Cemil; Ekici, Musa; Çağlayan, Mustafa Serdar; Öztürk, Muhammet İhsan; Baykam, Mehmet Murat
Background: – To prospectively evaluate the effects of rectal cleansing using a povidone-iodine (PI) and PI enhanced rectal enema on procedure-related infectious complications in patients undergoing transrectal prostate biopsy. Methods: – Between September 2023 and March 2024, patients scheduled for their first transrectal ultrasound-guided prostate biopsy due to elevated prostate-specific antigen levels, suspicious multiparametric prostate magnetic resonance imaging findings, or abnormal digital rectal examination were prospectively randomized into 2 groups (n = 60 each). All patients received standard prophylactic measures, including antibiotic prophylaxis starting 1 day prior to the procedure and rectal mucosal cleansing with PI immediately before biopsy. Both groups additionally underwent rectal enema administration. In the intervention group, a PI enhanced rectal enema was administered by adding PI to the sodium phosphate enema solution, whereas the control group received a sodium phosphate enema alone. Post-procedural complications, including hematuria, hematospermia, rectal bleeding, fever, acute urinary retention, hospitalization, sepsis, and urine and blood culture results, were recorded and compared using chi-square tests. Results: – In total of 120 patients were enrolled. The mean age in both groups was 66.6 years (± 7.20). Hospitalization due to febrile urinary tract infection occurred in 6 patients (10%) in the control group versus 2 patients (3.3%) in the study group. Positive urine cultures were found in 6 patients (10%) in the control group, while no positive cultures were detected in the study group, a statistically significant difference (P = .046). No significant differences were observed between the groups in terms of hematuria, hematospermia, rectal bleeding or acute urinary retention. Conclusion: – Rectal cleansing with a PI and PI enhanced rectal enema prior to transrectal prostate biopsy significantly reduced the rate of positive urine cultures. Although the reductions in febrile urinary tract infections and sepsis-related hospitalizations were not statistically significant, the overall trend suggests a potential benefit. Given that infectious complications are the most common adverse events associated with transrectal biopsy, this simple and low-cost intervention may improve patient safety.
- ArticleAçık Erişim2026ATP and Liv-52 Ameliorate Linezolid-Induced Liver Injury via Modulation of NF-κB/NLRP3 Pathways
Cerrah, Serkan; Baykan, Ahmed Ramiz; Sezgin, Esra Tuba; Huseynova, Gulbaniz; Karabacak, Elif; Tanas, Serdar; Baykan, Emine Kartal; Gunay, Murat; Gungor, Ali; Suleyman, Halis
Objective: Linezolid (LZD), an oxazolidinone antibiotic widely used against Gram-positive infections, has been associated with mitochondrial dysfunction and hepatotoxicity, particularly during prolonged use. This study aimed to investigate the protective effects of adenosine triphosphate (ATP) and Liv-52 against LZD-induced liver injury, with a focus on oxidative stress, inflammation, and necroptosis pathways. Methods: Twenty-four male Wistar rats were randomly assigned to four groups: healthy control (HG), LZD-treated (LZDG), Liv-52 + LZD (LVLZ), and ATP + LZD (ATLZ). Liv-52 (50 mg/kg, orally) and ATP (5 mg/kg, intraperitoneally) were administered prior to LZD (125 mg/kg, orally) for 14 days. Results: Following LZD administration, malondialdehyde (MDA) levels markedly increased, indicating oxidative stress, while total glutathione (tGSH), superoxide dismutase (SOD), and catalase (CAT) activities significantly decreased. Histopathological examination revealed pronounced hepatocellular damage accompanied by increased NF-κB, NLRP3, RIPK3, and MLKL expression, indicating activation of inflammatory and necroptotic pathways. Treatment with ATP and Liv-52 significantly ameliorated these biochemical, histopathological, and molecular alterations. Conclusions: Treatment with ATP and Liv-52 significantly attenuated oxidative stress, improved histopathological alterations, and suppressed the expression of inflammatory and necroptotic markers. Notably, ATP exhibited a more pronounced protective effect compared to Liv-52. In conclusion, LZD induces hepatotoxicity through oxidative stress-mediated inflammatory and necroptotic mechanisms, while ATP and Liv-52 confer hepatoprotection, with ATP showing superior efficacy.
- ArticleAçık Erişim2026First Report on the Phytochemical and Pharmacological Properties of Hedysarum yilmazunalii
Osma, Etem; Varol, Tuğçe; Şimşek, Samed; Altın, Sevgi; Yılmaz, Mustafa Abdullah; Aydın, Ali; Elveren, Müjgan; Kandemir, Ali
This study presents the phytochemical and biological evaluation of the species Hedysarum yilmazunalii Kandemir, F. Yıldız & H.İ. Turkoğlu. Methanolic extracts from leaves, stems, and roots were characterized by LC-MS/MS, and their antioxidant, enzyme inhibitory, and antiproliferative activities were investigated. The analyses showed organ-specific differences in phenolic composition, with quinic acid as the major compound in leaves together with higher levels of flavonoid glycosides, including astragalin and isoquercitrin. Aerial extracts showed strong enzyme inhibitory activity. Leaf and stem extracts exhibited cholinesterase inhibition, with IC50 values of 5.82 and 6.42 µg/mL for AChE and 5.41 and 5.50 µg/mL for BChE, respectively. The leaf extract showed α-glucosidase inhibitory activity (4.10 µg/mL), comparable to acarbose, and antioxidant activity (DPPH: 6.46 µg/mL), together with the highest phenolic (145.43 mg GAE/g extract) and flavonoid contents (87.20 mg QE/g extract). Antiproliferative assays demonstrated growth inhibition of lung and breast cancer cell lines, with the lowest GI50 values of 1.00 µg/mL for H1650 and 2.34 µg/mL for MDA-MB-231 cells. LDH cytotoxicity remained low (2.25%–12.59%), indicating limited membrane damage in both cancer and normal cells. These findings identify H. yilmazunalii as a source of compounds with antioxidant, neuroprotective, antidiabetic, and anticancer potential.
- ArticleAçık Erişim2026Evaluation of S100A4, CA-12, CA-9, Annexin A1/A2, and Galectin-3 in Prostate Cancer: Diagnostic and Prognostic Implications
Aslan, Rahmi; Huyut, Zübeyir; Keleş, Muhammed Fatih; Bedir, Nedim; Eryılmaz, Recep; Huyut, Mehmet Tahir; Saygın, Murat; Demir, Murat; Taken, Kerem
Objective: The limited specificity of prostate-specific antigen (PSA) increases the risk of unnecessary biopsies and overtreatment. This study aimed to evaluate the diagnostic and prognostic value of serum S100A4, along with CA-9, CA-12, Annexin A1/A2, and Galectin-3, in prostate cancer. Methods: This prospective case-control study, conducted between July 2024 and September 2025, included 80 patients with histopathologically confirmed prostate cancer and 40 age-matched healthy controls. Patients were stratified into low-risk (ISUP grades 1-2) and high-risk (ISUP ≥ 3) groups. Serum biomarker levels were measured using ELISA. Diagnostic and prognostic performance was assessed by receiver operating characteristic (ROC) analysis, with cut-off values determined using the Youden index. Logistic regression analyses were performed to identify independent biomarkers. Results: Serum CA-12 and CA-9 levels were significantly higher in prostate cancer patients than in controls (p = 0.004 and p = 0.032, respectively). Although S100A4 levels were lower in prostate cancer patients compared with controls, they were significantly higher in high-risk patients within the cancer cohort (p = 0.002). Logistic regression identified S100A4 (p = 0.032) and CA-12 (p = 0.004) as independent predictors of prostate cancer diagnosis. ROC analysis demonstrated excellent diagnostic accuracy for CA-12 (>52.9 ng/mL; AUC = 0.99) and S100A4 (<9.96 ng/mL; AUC = 0.98). For prognostic stratification, S100A4 (>8.27 ng/mL; AUC = 0.97) and CA-12 (>72.8 ng/mL; AUC = 0.91) effectively distinguished high-risk from low-risk disease. Conclusion: S100A4 and CA-12 exhibit strong diagnostic and prognostic performance in prostate cancer. When used alongside PSA, S100A4 and CA-12 may improve risk stratification in patients with suspected prostate cancer and help reduce unnecessary prostate biopsies. This work is licensed under a Creative Commons Attribution-Non Commercial 4.0 International License.
- ArticleYalnız künye2026A Novel Biomarker-Based Decision Support System for Pediatric Appendicitis Diagnosis: A Comparative Study of Ensemble Models Algorithms
Avci, Veli; Huyut, Mehmet Tahir; Velichko, Andrei; Belyaev, Maksim
Introduction: Acute appendicitis is the commonest cause of surgical abdominal pain, yet diagnosis in children remains challenging; delays increase the risk of perforation, peritonitis and sepsis. We sought to develop a rapid, inexpensive and interpretable clinical-decision support system (CDSS) that leverages routine blood tests (RBT) to assist early paediatric triage. Materials and Methods: In this retrospective single-centre study (January 2020–December 2024) we analysed 275 emergency-department encounters for abdominal pain (75 histology-confirmed appendicitis, 200 controls). The six-stage pipeline comprised (1) cohort selection; (2) exploratory logistic-regression screening of RBT variables; (3) training of Random Forest, Gradient Boosting and LightGBM ensembles (with/without SMOTE) under 10 × 10 stratified cross-validation; (4) SHAP-based feature interpretation; (5) exhaustive generation of every two- and three-parameter arithmetic biomarker from seven RBT features; and (6) derivation of probability-threshold curves and a three-zone rule tree for the top biomarker. Performance was reported with accuracy (ACC), Matthews correlation coefficient (MCC), AUC-ROC, sensitivity, specificity, F1-Score PPV and NPV. Results: Logistic regression and SHAP confirmed CRP, WBC and neutrophil count as strong positive predictors, whereas MPV and PDW were protective; PLT remained non-informative. All three ensemble classifiers surpassed 97% accuracy, 98% AUC-ROC and 0.93 MCC, with no gain from SMOTE. An extensive formula search, the best two-parameter marker was Neutrophil ÷ PDW (MCC = 0.73, specificity 95%). Its ensemble curve crosses P = 0.5 five times; practical cut-offs of < 0.633 (strongly indicate healthy) and > 0.794 (strongly indicate appendicitis) retain high NPV (∼91%) and PPV (∼86%). Among triple formulas that do not rely on PLT, the leading biomarker was CRP+WBC+Neutrophil (MCC = 0.85, PPV 92%, NPV 95%). The ensemble curve intersects at P = 0.5 at three points; values >27 strongly predict appendicitis, <23 indicates a healthy state, and values 23–27 leave a small uncertain band. A rule-based CDSS built on these two biomarkers correctly classified all controls (specificity 100%), sensitivity 95%, achieved 91% overall accuracy, and offers interpretable, electronic health records (EHRs)-ready cut-offs for paediatric appendicitis triage. Conclusion: Routine haematology-biochemistry data, interpreted through ensemble learning and engineered biomarkers, can deliver fast, transparent and highly accurate support for paediatric appendicitis triage. Given its zero false-positive rate, the proposed CDSS is best suited to in-hospital monitoring, where minimising false negatives is critical. Prospective multi-centre validation is warranted.
- ArticleYalnız künye2026Development of nimesulide-loaded pH-triggered ocular in situ gels: in vitro characterization, MTT assay, and Drazie test
Karakuyu, Nasif Fatih; Polat, Heybet Kerem; Unal, Sedat; Haydar, Muhammet Kerim; Mokhtare, Behzad; Aykurt, Furkan; Aksit, Aleyna
Objective: The present study created nimesulide-loaded in situ gels and assessed their potential application in ocular inflammation. Methods: Because in situ gels have a low viscosity, sodium alginate, and hydroxypropyl methylcellulose combined to create a nimesulide CD complex that is easy to apply. However, due to the gelation creation with pH, washing and removing it from the ocular surface becomes challenging. Production was carried out using different ratios of sodium alginate, hydroxypropyl methylcellulose, and hyaluronic acid. Results: Characterization studies led to the determination of the optimized formulation. The optimized formulation viscosity pH 5.5 = 547.3 ± 76cP, viscosity pH 7.4 = 8798.3 ± 254 cP, and pH = 5.4 ± 0.01 were obtained. In in vitro release experiments, approximately 53% of nimesulide was released by burst action within 2 h, followed by a controlled release over 12 h. Mathematical modeling of the formulations’ release kinetics revealed that they were consistent with the Korsemeyer-Peppas and Weibull models. It was determined that nimesulide-loaded in situ gels showed over 80% viability in the L929 cell line. Rats underwent no adverse conditions in Draize’s in vivo experiment. Conclusion: For this reason, the TB5 formulation may be a good choice when treating ocular inflammation. These findings may be supported by future efficacy studies using this formulation.
- ArticleAçık Erişim2026Carmofur inhibits ovarian cancer cell proliferation, migration, and invasion via disrupting the YAP-1/TEAD pathway: in vitro and in silico approaches
Cengiz, Celal; Vejselova Sezer, Canan; Gür, Bahri; Kutlu, Hatice Mehtap; Ayhanci, Adnan; Bakan, Nuri; Bayrakdar, Alpaslan; Cengiz, Mustafa
The current study aims to present a novel approach to alleviate the aggressiveness of ovarian cancer by pharmacological disruption of the Yes-Associated Protein (YAP) − 1/Transcriptional Enhancer Factor domain (TEAD) heterodimer structure using Carmofur. The Ovcar-3 cell line was incubated with progressively diluted Carmofur solutions. Proliferation inhibition was assessed using the MTT assay. Flow cytometry was employed to determine the pro-apoptotic properties. Cellular morphological alterations were assessed with confocal microscopy. The migration inhibition status was assessed via the scratch assay, whilst the colony formation inhibition level was evaluated using the colony assay. The western blotting technique was employed to determine YAP-1 expression levels. The disruptive impact of Ceramide and Carmofur on the YAP-1/TEAD heterodimer structure was elucidated via molecular modeling, considering three interfaces: the β-strand, the α-helix, and the Ω-loop. Furthermore, validation was conducted by assessing the disruptive impact of Verteporfin on the YAP-1/TEAD heterodimer. The findings indicated that Carmofur diminished cell viability in a concentration- and time-dependent manner, with IC50 values of 17.26 µM at 24 h, 10.15 µM at 48 h, and 9.22 µM at 72 h, respectively. Carmofur showed a 26.65% apoptotic effect at the IC50 value over 24 h. Additionally, Carmofur diminished the relative YAP-1 levels from 9.72 to 2.98 in the Ovcar-3 cell line and exhibited an anti-metastatic effect by suppressing colony formation and migratory capabilities. This is the first study to provide in silico and in vitro evidence that Carmofur induces apoptosis by disrupting the YAP-1/TEAD heterodimer, thereby inhibiting the proliferation and migration of Ovcar-3 cells.
- ArticleYalnız künye2026Clinical decision support system for detecting right ventricular dysfunction in acute pulmonary embolism: Explainable a new Binary Rule Search (BRS) ensembles and robustness evaluation
Huyut, Mehmet Tahir; Velichko, Andrei; Belyaev, Maksim; Izotov, Yuriy; Tosun, Mustafa; Sertoğullarından, Bünyamin; Karaoğlanoğlu, Şebnem; Demir, Abdussamed Yasin; Korzun, Dmitry
Background Right-ventricular dysfunction (RVD) in acute pulmonary embolism (PE) carries excess short-term mortality; fast, transparent risk stratification is needed. Objective To develop and evaluate an interpretable Binary Rule Search (BRS) framework for RVD detection on a fully binarized clinical dataset, emphasizing conservative robustness. Methods We analyzed a single-center cohort ( N = 363; development = 250, external validation = 113) recoded into 0/1 predictors. For subset sizes k = 1–5, a Rust BRS searched bit-mask rules maximizing Matthews correlation coefficient (MCC) on development data and was assessed over 500 stratified 250/113 hold-out repeats. We report MCC with bootstrap 95 % CIs plus sensitivity, specificity, precision, F1, and AUC-ROC. Robustness was summarized by the Stability-Bound Rule Score (SBRS). Results Performance increased with k; triplets offered the best parsimony–robustness balance, with modest conservative gains for quintets. The strongest quintet by point estimate was S{Age65_79; ThromMain; ThromBilat; DVTuni; Malig} (MCC = 0.326; 95 % CI 0.152–0.498). A balanced k = 4 rule, S{SexMale; ThromMain; Hypertens; HeartFail}, achieved Sensitivity 0.791, Specificity 0.557, F1 0.630, AUC-ROC 0.674 on external validation. Decision-tree checks yielded high lower-bound performance (e.g., L95 = 0.302 for S{ThromMain; DVTdist; COPD}), whereas LogNNet often matched mean MCC but showed consistently lower L95, indicating greater dispersion. PFI highlighted a thrombus-centric signal (ThromMain/ThromBilat/DVT) with meaningful secondary contributions from heart failure and hypertension. Conclusions Interpretable BRS ensembles deliver clinically acceptable, conservatively bounded performance using few binary predictors and transparent logic, supporting clinician-facing decision support in resource-constrained settings. We provide rule masks, uncertainty summaries, and an offline CDSS prototype; prospective multicenter validation is warranted.
- ArticleYalnız künye2026Bee venom–derived exosomes combined with metronidazole: antimicrobial effect on Campylobacter jejuni and fibroblast wound healing model
Celebi, Ozgur; Celebi, Demet; Baser, Sumeyye; Genc, Sidika; Taghizadehghalehjoughi, Ali
Background: Campylobacter jejuni is one of the most common causes of bacterial gastroenteritis worldwide, and its increasing resistance to antibiotics highlights the urgent need for alternative therapeutic strategies. Methods: This study investigates the antibacterial, antibiofilm, anti-inflammatory, and wound-healing effects of bee venom-derived exosomes (BVE), alone and in combination with metronidazole, against Campylobacter jejuni. Exosomes were isolated from bee venom. Their morphology was confirmed using scanning electron microscopy. Antimicrobial activity was assessed using disc diffusion, minimum inhibitory concentration, minimum bactericidal concentration, and fractional inhibitory concentration methods. A fibroblast wound infection model was developed to evaluate the effect on cell viability (MTT assay), oxidative stress (TOS/TAC), cytokine release (IL-1β, IL-10), and apoptosis-related gene expression (BAX, BCL-2). Results: The results demonstrated that both BVE and metronidazole possess antibacterial and antibiofilm activity, with the combination treatment showing additive effect (inhibition zone: 20 mm, biofilm inhibition: 82.6%). The combined treatment significantly decreased oxidative stress markers and IL-1β levels, while increasing IL-10 and total antioxidant capacity. Gene expression analysis showed that the treatment reduced pro-apoptotic BAX levels and upregulated anti-apoptotic BCL-2 expression, contributing to improved cell survival and wound regeneration. Conclusion: Bee venom-derived exosomes, especially when combined with metronidazole, exhibit strong potential as an alternative or adjunct therapeutic approach against Campylobacter jejuni infection. These findings suggest that BVE may offer a novel strategy to combat antibiotic resistance and promote tissue repair in bacterial gastroenteritis.
