Yazarlar
- Suleyman, Halis(167)
- Mammadov, Renad(95)
- Türkeş, Cüneyt(84)
- Aydin, Sonay(83)
- Altuner, Durdu(74)
- Suleyman, Bahadir(68)
- Isik, Arda(67)
- Beydemir, Şükrü(66)
- Kantarci, Mecit(64)
- Kurt, Nezahat(57)
- Demir, Yeliz(54)
- Bulut, Seval(51)
- Arslan, Yusuf Kemal(50)
- Sofu, Hakan(50)
- Coban, Taha Abdulkadir(49)
- Karakeçili, Faruk(47)
- Altay, Ahmet(46)
- Huyut, Mehmet Tahir(46)
- Aydin, Suleyman(45)
- Türkmenoğlu, Burçin(45)
- Kuzucu, Mehmet(40)
- Yazici, Gulce Naz(40)
- Peker, Kemal(39)
- Algul, Oztekin(38)
- Cicek, Betul(37)
- Mertoglu, Cuma(36)
- Camurcu, Yalkin(35)
- Suleyman, Zeynep(34)
- Bozkurt, Aliseydi(32)
- Mendil, Ali Sefa(32)
- Gunay, Murat(31)
- Gözcü, Sefa(31)
- Köksal, Ekrem(31)
- Yuca, Hafize(31)
- Dilek, Esra(30)
- Karakurt, Papatya(30)
- Ozcicek, Fatih(30)
- Süleyman, Halis(30)
- Onk, Didem(29)
- Ucpunar, Hanifi(29)
Yayınlar
- ArticleAçık Erişim2012Effects of levosimendan on TNF-alpha, BNP and MMP-1 in patients with heart failure with anemia
Büyüklü, Mutlu; Kürüm, Akif Turhan; Tatlís, Ersan; Set, Turan
Background: Levosimendan is known with its two-sided effects of strengthening myofibril contraction without increasing myocardial oxygen demand. Anemia is a deteriorating situation that causes increase of drug dosing in patients with heart failure. Objectives: In this study, we compared the effectiveness of levosimendan treatment in decompensated heart failure patients with or without anemia. Methods: Twenty-three anemic patients having class 3 or 4 heart failure according to New York Heart Association (NYHA) and an ejection fraction of below 35% were included to the study. Another 23 patients with the same cardiac diagnosis but without anemia served as control group. Twenty-four hours levosimendan treatment was added to the traditional heart failure treatment of these patients. Samples were taken to measure serum tumor necrotizing factor alpha (TNF-alpha), aminoterminal pro-brain natriuretic peptide (NT-proBNP) and matrix metalloproteinase-1 (MMP-1) levels before and after the administration. Results: There was no significant difference between serum TNF-alpha and MMP-1 levels before and after the treatment (p>0.05). Although NT-proBNP level decreased in both groups after the treatment this was not statistically significant (p=0.531 and p=0.913 for anemia and control groups respectively). Significant restoration of functional capacity was seen in both groups assessed according to NYHA (p<0.001 and p=0.001 for anemia and control groups respectively). Conclusion: Levosimendan treatment shows similar effects in heart failure patients with anemia to that of patients without anemia. However, the early effect of this treatment on TNF-alpha, NT-proBNP and MMP-1 levels is not evident. It provides significant improvement in functional capacity without influence from anemia.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2012Effects of some drugs on human cord blood erythrocyte carbonic anhydrases I and II: An in vitro study
Cankaya, Murat; Aktas, Mehmet; Kuzucu, Mehmet; Gül, Ilhami; Coban, T. Abdulkadir
In the present study, we purified hcbCA I and II from human cord blood erythrocytes using by Sepharose-4B-l tyrosine-sulfanilamide affinity gel chromatography. Also, it was checked the inhibition effects of ampicillin sulfate, ceftriaxone, ceftizoxime and ranitidine on hcbCA I and hcbCA II. IC50 values for ceftriaxone, ceftizoxime and ranitidine were found to be 27.l, 79.4 and 55.5 M for hcbCA I and of 21.0, 79.1 and 66.1 M for hcbCA II, respectively. According to these results, Ampicillin sulfate inhibited only hcbCAII and IC50 values of this antibiotic was found to be 56.8 M. All these substances were found non-competitive inhibitors. It is important to study the inhibition effects of these drugs on hcbCA I and II izoenzymes. Because, pregnant woman is take all of these substance. For this reason, these drugs should be carefully used and the dosage should be very well ordered to minimize side effects.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2012The inhibitory effects of L-adrenaline on lactoperoxidase enzyme purified from bovine milk
Şisecioglu, Melda; Gülçin, Ilhami; Çankaya, Murat; Ozdemir, Hasan
L-Adrenaline belongs to a group of compounds known as catecholamines, which play an important role in the regulation of the physiological process in living organisms. In the present study, the inhibitory effect of L-adrenaline on lactoperoxidase was examined. Lactoperoxidase (E.C.1.11.1.7) was purified from bovine milk with three consecutive steps: Amberlite CG-50 resin, CM-Sephadex C-50 ion-exchange, and Sephadex G-100 gel filtration chromatography. Lactoperoxidase was purified with a yield of 42.18%, a specific activity of 30.33 EU/mg proteins, and 20.77 purification fold. Enzyme purity was determined with SDS-PAGE, where a single band was observed. The Rz (A 412/A280) value for lactoperoxidase was 0.9. The effect of L-adrenaline on lactoperoxidase was determined using ABTS as a chromogenic substrate. The half maximal inhibitory concentration (IC50) value and an inhibition constant (Ki) values for L-adrenaline were 34.5 and 2.26 μM, respectively. L-Adrenaline was found to be a non-competitive inhibitor.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2012Examination of the tissue ghrelin expression of rats with diet-induced obesity using radioimmunoassay and immunohistochemical methods
Aydin, Suleyman; Sahin, Ibrahim; Ozkan, Yusuf; Dag, Ersel; Gunay, Ahmet; Guzel, Saadet Pilten; Catak, Zekiye; Ozercan, Mehmet Resat
Currently, obesity is an important health problem in all countries, both developed and developing. Dietary habits and neurohormonal imbalances play a critical role in obesity. Circulating amounts of ghrelin, which is a neurohormonal hormone, decrease with obesity and increase with weight loss. Although it is known that both mRNA and peptide version of the ghrelin hormone are expressed in almost all tissues of both humans and animals, it is not known how obesity changes the expression of this hormone in the tissues, with the exception of the gastrointestinal system tissues. Therefore, the objective of the present study is to show how diet-induced obesity in rats changes ghrelin expression in all system tissues, and thus, to shed light on the etiopathology of obesity. The study included 12 male and 12 female 2-month-old Wistar albino species rats. The animals in the control group were fed on standard rat pellet, while those in the experiment group were fed ad libitum on a cafeteria-style diet for 2 months. When their body mass index reached 1 g/cm 2, diet-induced obese (DIO) rats were sacrificed in a sterile environment after one night fasting. Ghrelin localizations in the tissues were studied immunohistochemically using avidin-biotin-peroxidase complex (ABC) method, while tissue ghrelin amounts were analyzed using radioimmunoassay (RIA) method. When the ghrelin amounts in the urogenital system (with the exception of kidney tissues), sensory organs, respiratory system, immune system, skeletal muscle system, cardiovascular system, nervous system, and adipose tissue of rats analyzed by RIA method were compared to those in the control group, tissue ghrelin amounts in the DIO group were found lower. Immunohistochemical findings which showed a similar fall in ghrelin concentrations in the tissues were parallel to RIA results. In addition, ghrelin was shown to be synthesized in the cardiovascular system, heart muscle cells, tails of the sperms, hair follicles, lacrimal glands, tongue, and teeth of rats for the first time in this study and ghrelin syntheses in these tissues were found to decrease in obesity. Nutritional obesity is among the most common causes of obesity and the findings we have obtained through diet-induced obesity will contribute to the illumination of the etiopathology of obesity.
RDL Kalıcı Kaydıİncele - Review ArticleAçık Erişim2012The past and present of paraoxonase enzyme: Its role in the cardiovascular system and some diseases
Aydin, Suleyman; Sahin, Ibrahim; Aydin, Suna; Aksoy, Aziz; Citil, Cihan
Although paraoxonase is synthesized in many tissues including the heart, colon, kidneys, lungs, small intestines and brain, its major locus of synthesis is the liver. PON1 is in close association with apolipoproteins and protects LDL against oxidation. It was reported that PON1 quantities dropped to 40 times lower than normal in cardiovascular diseases and diseases like diabetes, ulcerative colitis, Crohn's disease, chronic renal failure, SLE, Behcet's disease, cancer, hepatitis B, obesity, metabolic syndrome, Alzheimer's and dementia. It is speculated that the concerning decline in serum PON1 amount results from single nucleotide polymorphism in the coding (Q192R, L55M) and promoter (T-108C) sites of the PON1 gene. Additionally, circulating amounts of PON1 are affected by vitamins, antioxidants, fatty acids, dietary factors, drugs, age and lifestyle. This collection attempts to review and examine the past and present studies of paraoxonase and its relation with the cardiovascular system and some relevant diseases.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2012Life span effects of Hypericum perforatum extracts on Caenorhabditis elegans under heat stress
Kiliçgün, Hasan; Göksen, Gülden
Background: The beneficial effects of antioxidants in plants are mainly extrapolated from in vitro studies or short-term dietary supplementation studies. Due to cost and duration, relatively little is known about whether dietary antioxidants are beneficial in whole animals′ life span or not. Materials and Methods: To address this question, under heat stress (35°C), Hypericum perforatum was extracted with petroleum ether and the nematodes Caenorhabditis elegans exposed to three different extract concentrations (1mg/mL, 0.1mg/mL, 0.01mg/mL) of H. perforatum. Results: We report that Hypericum perforatum extracts did not increase life span and slow aging related increase in C. elegans. Moreover, one fraction (1mg/mL) increased declines of C. elegans life span and thermotolerance. Conclusion: Given this mounting evidence for life span role of H. perforatum in the presence of heat stress in vivo, the question whether H. perforatum acts as a prooxidant or an antioxidant in vivo under heat stress arises.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2012The effect of education given to patients with type 2 diabetes mellitus on self-care
Karakurt, Papatya; Kaşikçi, Maǧfiret Kara
This research was performed as a single group pretest-posttest experimental design to determine the effect of education given to patients with type 2 diabetes mellitus on self-care. The research was performed between October 2007 and June 2008 in the Internal Medicine Outpatient Clinics located in A and B Blocks of Erzincan State Hospital. The research population included 100 patients with type 2 diabetes mellitus who attended the above mentioned units between the specified dates and met the inclusion criteria. Patients were subjected to a pretest using a patient identification form, Diabetes Self-Care Scale (DSCS) form in Turkish language and metabolic control parameters. A statistically significant difference was found between the mean values of pre-education and posteducation DSCS scores with an increase in mean posteducation scores. These results demonstrate that the education given to patients improves their self-care and metabolic control variables.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2012Nesfatin-1 and other hormone alterations in polycystic ovary syndrome
Deniz, Rulin; Gurates, Bilgin; Aydin, Suleyman; Celik, Husnu; Sahin, Ibrahim; Baykus, Yakup; Catak, Zekiye; Aksoy, Aziz; Citil, Cihan; Gungor, Sami
Polycystic ovary syndrome (PCOS) is commonly characterised by obesity, insulin resistance (IR), hyperandrogenemia and hirsutism. Nesfatin-1 a recently discovered hormone, acts upon energy balance, glucose metabolism, obesity and probably gonadal functions. This study was to evaluate the circulating levels of nesfatin-1 in patients with PCOS (n = 30) and in age and body mass index (BMI)-matched controls (n = 30). PCOS patients had significantly lower levels of nesfatin-1 (0.88 ± 0.36 ng/mL) than healthy controls (2.22 ± 1.14 ng/mL). PCOS patients also had higher gonadotropin and androgen plasma concentrations, Ferriman-Gallwey scores, blood glucose levels and a homeostasis model of assessment-IR index (HOMA-IR) index than in healthy women. Correlation tests in PCOS subjects detected a negative correlation between nesfatin-1 levels and BMI, fasting blood glucose, insulin levels and a HOMA-IR index. Lower nesfatin-1 concentration may plays a very important role in the development of PCOS.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2012Sulfapyridine-like benzenesulfonamide derivatives as inhibitors of carbonic anhydrase isoenzymes I, II and VI
Alp, Cemalettin; Özsoy, Şeyda; Alp, Nurdan Alcan; Erdem, Deryanur; Gültekin, Mehmet Serdar; Küfrevioǧlu, Ömer Irfan; Şentürk, Murat; Supuran, Claudiu T.
The inhibition of two human cytosolic carbonic anhydrase (hCA, EC 4.2.1.1) isozymes I, II and human serum isozyme VI, with a series of tosylited aromatic amine derivatives was investigated. The KI ranges of compounds 1-14 and acetazolamide against hCA I ranged between 1.130 and- 448.2 μM, against hCA II between 0.103 and- 14.3 μM, and against hCA VI ranged between 0.340 and- 42.39 μM. Tosylited aromatic amine derivatives are thus interesting hCA I, II and VI inhibitors, and might be used as leads for generating enzyme inhibitors eventually targeting other isoforms which have not been assayed yet for their interactions with such agents.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2012Genotoxic evaluation of selective serotonin-reuptake inhibitors by use of the somatic mutation and recombination test in Drosophila melanogaster
Gürbüzel, Mehmet; Oral, Elif; Kizilet, Halit; Halici, Zekai; Gulec, Mustafa
This study evaluated different concentrations of selective serotonin-reuptake inhibitors (citalopram and sertraline) for genotoxicity by use of the somatic mutation and recombination test (SMART) in Drosophila melanogaster. Three-day-old larvae, trans-heterozygous for the multiple wing hairs (mwh) and flare (flr3) genes were treated with these two compounds. Two recessive markers were located on the left arm of chromosome 3, i.e. 'multiple wing hairs' (mwh) in map position 0.3 and 'flare-3' (flr3) at 38.8, while the centromere was located in position 47.7. SMART is based on the loss of heterozygosity, which may occur through various mechanisms, such as mitotic recombination, mutation, deletion, half-translocation, chromosome loss, and non-disjunction. Genetic changes occurring in somatic cells of the wing's imaginal discs, cause the formation of mutant clones on the wing blade. The results of this study show that citalopram had a genotoxic effect in the Drosophila SMART. Sertraline, however, did not show any genotoxic effect in balancer heterozygous wings. This study concluded that more information is needed to be certain regarding the mutagenic effects of sertraline.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2012Hierarchical factor structure of the Turkish version of the posttraumatic growth inventory in a normal population; [Travma sonrası büyüme envanteri'nin Türkçe versiyonunun normal toplumda hiyerarşik faktör yapısı]
Kaǧan, Mücahit; Güleç, Mustafa; Boysan, Murat; Çavuş, Hayati
AIM: Negative consequences of traumatic experiences, such as depression, anxiety, or dissociative symptoms, etc. have been reported by many researchers. However, it is proposed that stressful events not only lead to poor psychological outcomes but also may trigger positive changes. Several instruments gauging posttraumatic benefits have been developed to examine the effects of factors that may promote positive psychological outcomes in the aftermath of stressful events. The Posttraumatic Growth Inventory (PTGI) is one of the prominent instruments that assess posttraumatic positive changes. In this study we aimed to assess psychometric properties of the Turkish version of the PTGI. METHOD: This study differed to an extent from previous studies concerning the features of the PTGI. We used a dispositional form of the instrument in a sample recruited from high school and university students. Our data were collected from 723 volunteers. 367 subjects were males (50.76%) and 356 subjects were females (49.24%). Also we did not specify any selection criteria in recruiting subjects owing to their adverse life experiences with a presumption that stressful life events are not uncommon in normal population. We administered to participants a dispositional form of the Posttraumatic Growth Inventory and the Personal View Survey-III. Item statistics for the measure were computed. We performed an explanatory factor analysis by using principal components with promax rotation and a confirmatory factor analysis by using structural equation modeling. Since the factor inter-correlations were higher than.40 we computed Schmid-Leiman transformation to obtain second-order general factor loadings. Inner consistencies and 15-day test-retest intracorrelations were calculated. RESULTS: Item discrimination indexes ranged from.28 to.72. Promax rotated principal components analysis pointed out a three-factor structure. It was found in model testing with structural equation modeling that three-factor structure was valid for the Turkish version of the PTGI. After computing second-order factor loadings, we detected that general factor accounted for 64 percentage of the total variance. Three subscales of the measure were the Changes in Self-Perception, Changes in Philosophy of Life, and Changes in Relationship. Internal consistency for the Changes in Self-Perception subscale was 0.88, for the Changes in Philosophy of Life subscale was 0.78, for the Changes in Relationship was 0.77, and for the overall items was 0.92. 15-day test-retest intra-correlation for the composite scores was 0.83 and intra-correlations for the subscale scores ranged from 0.70 to 0.85. CONCLUSION: Turkish version of the measure revealed a three-factor first-order structure. However, it seems that the concept of posttraumatic benefits measured by the PTGI has a tendency to represent a unidimensional psychological construct in Turkish sample. Since the three-factor structure was validated, three-subscales may also be used to make an extensive assessment instead of composite scores. The PTGI is a valid and reliable measure to be used in research purposes among Turkish individuals.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2012Knowledge, behavior and beliefs related to cervical cancer and screening among Turkish women
Reis, Nesrin; Bebis, Hatice; Kose, Sevinc; Sis, Asli; Engin, Raziye; Yavan, Tulay
Objective: The aims of this study were to explore Turkish women's knowledge, behavior and beliefs related to cervical cancer and screening. Methods: The study was performed in two cities in the East of Turkey between September 2009 and April 2010, with a sampling group of 387 women. Data were collected by means of an interview form with the Health Belief Model Scale for Cervical Cancer and Pap Smear Test - Turkish Version. Results: Women in the research group were found to have poor knowledge, inadequate health behavior and low/medium level false beliefs regarding cervical cancer screening. There was relation between health beliefs and characteristics of women and particularly education (F = 10.80, p = 0.01). Similarly, it was found that Pap smear barriers were influenced by demographic characteristics and that women with low-level education (p = 0.001), divorced women (p = 0.05), women with low-income(p = 0.05), women who gave their first birth when they were 18 or younger (p = 0.05) and women not applying any contraceptive method at all (p = 0.01) were determined to have negative Pap smear barriers. Conclusions: Primarily the knowledge, attitudes and beliefs of women in the target group should be evaluated to increase their participation in cervical cancer screening and to prepare effective education strategies.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2012Investigation of radioactivity level in soil and drinking water samples collected from the city of Erzincan, Turkey
Yalcin, P.; Terzi, M.; Bastug, A.; Taskin, H.; Varinlioglu, A.; Tasdelen, B.; Kam, E.; Bozkurt, A.
This study is part of an effort to assess the level of background radiation for Erzincan Province of eastern Turkey. Radionuclide activity concentrations in soil samples were measured through gamma-ray spectrometry and the average activities were determined as 8.93, 11.39, 281.94, and 9.52 Bq/kg for the radionuclides 238U, 232Th, 40K, and 137Cs, respectively. The average annual effective dose from these natural radioactivity sources ( 238U series, 232Th series and 40K,) was calculated to be 27.9 μSv. Radioactivity levels in drinking and potable water samples were studied using a multi-channel low level proportional counter. The average gross alpha activity concentration was found to be 0.0477 Bq/L (min. 0.007 Bq/L; max. 0.421 Bq/L) and the average gross beta activity was measured as 0.104 Bq/L (min. 0.008 Bq/L; max. 1.806 Bq/L). These values lead to an average annual effective dose of 9.75 μSv from the alpha emitters and 56.34 μSv from the beta emitting radionuclides in water. The radioactivity levels in the water samples investigated were found to comply with the reference levels recommended by WHO and the regulations set forth by the Turkish Health Ministry.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2012Comparison of central corneal thickness measurements by Galilei Dual-Scheimpflug Analyzer® and ultrasound pachymeter in myopic eyes
Yeter, Volkan; Sönmez, Baris; Beden, Ümit
■ BACKGROUND AND OBJECTIVE: To compare the central corneal thickness (CCT) measurements by Galilei Dual-Scheimpflug analyzer (GSA) (Ziemer Group, Port, Switzerland) and ultrasound pachymeter (UP) in myopic eyes. ■ PATIENTS AND METHODS: In this prospective study, CCT of 161 myopic eyes of 81 refractive surgery candidates (35 female, 46 male; mean age: 23.18 ± 4.08 years) were measured by GSA and UP consecutively. The data were analyzed statistically by paired t test, Bland-Altman plot, and Pearson correlation test to assess the agreement of the measurements. ■ RESULTS: The mean CCTs obtained by GSA and UP were 559.85 ± 30.87 and 560.41 ± 34.45 μm, respectively. No significant difference was found between the measurements (P = .684) and regression analysis showed a high correlation between the measurements obtained with both devices (r = 0.86; P < .001). A strong agreement between the two methods was also found by Bland-Altman plot. ■ CONCLUSION: In myopic eyes, the CCT measurements of GSA and UP are similar and highly correlated. Because of high agreement between these devices, the GSA is a non-contact method that may be an alternative to UP for measurement of CCT.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2012Changes in serum obestatin, preptin and ghrelins in patients with Gestational Diabetes Mellitus
Baykus, Yakup; Gurates, Bilgin; Aydin, Suleyman; Celik, Husnu; Kavak, Burcin; Aksoy, Aziz; Sahin, Ibrahim; Deniz, Rulin; Gungor, Sami; Guzel, Saadet P.
Objectives: The present study aims to establish the levels of acylated ghrelin, desacylated ghrelin, obestatin and preptin, during pregnancy and the postpartum period in pregnant women with Gestational Diabetes Mellitus (GDM) and healthy pregnancy women. Design and methods: The study registered 20 pregnant women with GDM and 20 healthy pregnant women. Fasting venous blood samples were collected from all cases between weeks 24 and 28 of pregnancy and after 24. h postpartum. Hormones were analyzed using ELISA method. Results: Serum acylated ghrelin (p:0.001), desacylated ghrelin (p:0.001), obestatin (p:0.006) and preptin (p:0.001) levels were all found statistically higher in both groups during the postpartum period, when compared to the pregnancy period. A positive correlation was established between desacylated ghrelin and acylated ghrelin (p:0.008), desacylated ghrelin and preptin (p:0.012) and preptin and insulin (p:0.039) in the GDM group during pregnancy. Conclusions: The studied hormones (especially desacylated ghrelin and obestatin) are critical in GDM pathophysiology based on the comparison of measure after and before the delivery.
RDL Kalıcı Kaydıİncele - LetterYalnız künye2011We built the first public radial angiography laboratory contrary to the hesitation that conventional coronary angiography could have been performed in Erzincan
Vural, Mutlu
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2011Radiological characterisation of Artvin and Ardahan provinces of Turkey
Kucukomeroglu, B.; Yesilbag, Y.O.; Kurnaz, A.; Celik, N.; Cevik, U.; Celebi, N.
Indoor radon concentration measurements were carried out and corresponding annual effective doses due to exposure to indoor radon were determined in Artvin and Ardahan provinces located in the eastern part of Turkey. The measurements were performed for four seasons in order to determine the seasonal fluctuations mostly observed in indoor environments. Indoor radon concentration values were observed to range from 21 to 321 Bq m-3 for the Artvin province and from 53 to 736 Bq m-3 for the Ardahan province. It was observed that minimum indoor radon concentration values were obtained in summer, while the highest ones were observed in winter. Indoor radon concentration values of the current study were compared with those of other provinces in Turkey. As elevated indoor radon concentrations are mostly correlated with high 238U activity concentrations in soil, a total of 57 and 33 soil samples were collected from the Artvin and Ardahan provinces, respectively, to determine 238U activity concentration as well as the concentration of 232TH and 40K-naturally occurring radionuclides. It was also observed that soil samples collected from the study areas contained 137Cs as an artificial radionuclide.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2011Effects of ceftazidime pentahydrate, prednisolone, amikacin sulfate, ceftriaxone sodium and teicoplanin on bovine milk lactoperoxidase activity
Sisecioglu, M.; Uguz, M.T.; Cankaya, M.; Özdemir, H.; Gülçin, I.
The antibiotics are substances that kill or inhibit the growth of bacteria. Most antibiotics are modified chemically from original compounds found in nature. The antibiotics were frequently used for antimicrobial therapy. The main objective of this study was to determination of the effects of some antibiotics such as ceftazidime pentahydrate, prednisolone, amikacin sulfate, ceftriaxone sodium and teicoplanin on bovine milk lactoperoxidase activity were investigated in this study. Lactoperoxidase was purified using Amberlite-CG-50 resin, CM Sephadex-C-50 ion-exchange and Sephadex-G-100 gel filtration chromatographies from bovine milk. 2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) was used as a chromogenic substrate for spectrophotometric determination of lactoperoxidase activity. Lactoperoxidase was purified 19 fold with a yield of 24.5%. All of the used antibiotics exhibited inhibitory effects on the enzyme and displayed competitive type of inhibition mechanism. IC50 values of ceftazidime pentahydrate, prednisolone, amikacin sulfate, ceftriaxone sodium and teicoplanin were 0.048, 0.053, 0.26, 0.29 and 1.016 mM, respectively. Ki constants were calculated as 0.018±0.0035 mM, 0.019±0.0005 mM, 0.04±0.015 mM, 0.10±0.055 mM and 0.13±0.022 mM for ceftazidime pentahydrate, prednisolone, amikacin sulfate, ceftriaxone sodium and teicoplanin, respectively. Present study showed that ceftazidime pentahydrate exhibited much higher inhibitory effect at lower concentrations compared to other antibiotics used.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2011Ghrelin expression of endometrium hyperplasia and endometrioid carcinoma
Dagli, Adile F.; Aydin, Suleyman; Kocdor, Hilal; Gurates, Bilgin; Sahin, Ibrahim; Catak, Zekiye; Ozercan, Mehmet R.; Ozercan, Ibrahim. H.
Background. Endometrium carcinoma ranks fourth among female carcinomas. Therefore, early diagnosis of endometrium pre-malignant lesions is emphasised, and attempts are made to identify the risk factors. Since hyperplasias, particularly those with atypia, are held responsible for the development of the most common endometrium carcinomas, it is important to definitely distinguish between well-differentiated carcinomas and hyperplasia with atypia. In the present study, we aimed to explore whether ghrelin expression had a role in distinguishing between benign, pre-malignant and malignant lesions of endometrium. Methods. Tissue ghrelin expressions of a total of 60 cases, who were diagnosed in the Pathology Department Laboratory of Fiirat University Medical School, and of whom 10 were in the proliferation phase, 10 had simple hyperplasia without atypia, 10 had simple hyperplasia with atypia, 10 had complex hyperplasia without atypia, 10 had complex hyperplasia with atypia and 10 had endometrioid carcinoma cases, were examined using immunohistochemical method. Additionally, tissue samples were homogenised to analyse tissue ghrelin levels in the supernatants according to RIA method. Samples from the parotid glands were used as positive control for ghrelin. Cells that exhibited cytoplasmic staining with ghrelin antibody were evaluated as positive. Results. Immunohistochemical examination showed that ghrelin expression increased markedly in the proliferation phase, relative to hyperplasias and carcinoma. These results were parallel to ghrelin levels in tissue supernatants. Immunohistochemical and RIA analysis results indicate that ghrelin expression either markedly decreases or is entirely depleted in endometrial carcinomas. Conclusions. Therefore, we think that ghrelin expression can be useful in differentiating not only endometrium carcinomas from benign lesions but also complex hyperplasias with atypia, which pose diagnostic difficulties.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2011Stress, self-perception and interpersonal style in patients with physical Illnesses
Durak Batigün, Ayşegül; Hisli Şahin, Nesrin; Karsli Demirel, Esra
Objective: This study aimed to investigate the role of self-perception, interpersonal style, and anger in the context of stress in patients with physical illnesses, including coronary artery disease, gastrointestinal disorders, dermatological disorders, and diabetes. Materials and Methods: The study sample included patients with physical illnesses (n = 124) and healthy controls (n = 209). Symptoms of stress, self-perception, interpersonal style, and anger were evaluated using the Stress Symptoms Scale, Social Comparison Scale, Interpersonal Style Scale, and Multidimensional Anger Questionnaire, respectively. The role of self-perception, interpersonal style, and anger in stress experienced in the patients and controls was compared. Results: The patients had higher stress symptoms, perceived themselves more negatively, had more problematic interpersonal styles, and more intense anger than the controls. The higher stress symptoms in the patients and the lower symptoms in the controls was predicted by 4 common variables3/4dissatisfaction with life, dissatisfaction with interpersonal relationships, negative self-perception, and aggressive expression of anger. Another predictive variable in the psychosomatic group was age, whereas in the control group gender and internalized anger were predictive variables. Conclusion: As the physical illnesses had by those in the patient group are stress-related, inclusion of psychosocial interventions in treatment protocols, such as communication skills, and stress and anger management training, and interventions aimed at increasing a positive self-perception might improve their QoL.
RDL Kalıcı Kaydıİncele
