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- Suleyman, Halis(167)
- Mammadov, Renad(95)
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- Huyut, Mehmet Tahir(46)
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- Kuzucu, Mehmet(40)
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- Algul, Oztekin(38)
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- Mertoglu, Cuma(36)
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- Dilek, Esra(30)
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- Ozcicek, Fatih(30)
- Süleyman, Halis(30)
- Onk, Didem(29)
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Yayınlar
- MakaleAçık Erişim2025Dual-functionality of Hibiscus sabdariffa-CuO nanoparticles in chemotherapy and textile screen-printing on cellulose-based textiles
Tüzün, Elif; Erol, Ayşe; Kalındemirtaş, Ferdane Danışman; Özbaş, Fatih; Sert, Esra; Soomro, Razium Ali; Nafady, Ayman; Karakuş, Selcan
The dual-functional nanostructures show great promise for biomedical applications, exhibiting selective cytotoxicity against cancer cells while also serving as a crucial component in textile screen-printing for smart materials. In this study, we successfully synthesized polyethylene glycol-hibiscus extract copper (II) oxide nanoparticles (PEG/HS/CuO NPs) using a simple one-step sonosynthesis method that leverages ultrasonic irradiation. Comprehensive characterization of the synthesized PEG/HS/CuO NPs was performed using transmission electron microscopy (TEM), X-ray diffraction (XRD), scanning electron microscopy (SEM) coupled with energy-dispersive X-ray (EDX) analysis, and Fourier-transform infrared spectroscopy (FTIR). The incorporation of PEG/HS/CuO NPs into guar gum photochromic solution (GP) caused a significant color change after 6 ± 1 min of UV light exposure and resulted in visible coloration on cellulose-based textiles after screen printing, providing an alternative strategy for smart fabrics. Moreover, cytotoxicity experiments demonstrated the selective toxicity of green PEG/HS/CuO NPs against cancer cells. In this study, the human colon cancer cell line HCT116, breast cancer cell line MCF-7, and normal HUVEC cells were examined. PEG/HS/CuO NPs NPs induced apoptosis, cell cycle arrest, and down-regulation of CD44 antibody expression in MCF-7 cells, highlighting their potential as effective chemotherapy agents.
- MakaleAçık Erişim2025Irisin and adropin decrease in preobese or obese individuals with nonalcoholic fatty liver disease
Mertoğlu, Cuma; Örek, Yaprak Şule; Albayrak, Bülent; Arslan, Yusuf Kemal; Çoban, Abdulkadir
Background/aim: Nonalcoholic fatty liver disease (NAFLD) is a metabolic disease that is commonly observed in individuals with obesity. The present study investigates the irisin and adropin levels of sera in preobese or obese adults with NAFLD. Materials and methods: Included in the study were 89 patients who were categorized into four groups: Group 1: 25 normal-weight individuals without NAFLD (Control group); Group 2: 17 preobese or obese individuals without NAFLD; Group 3: 24 preobese or obese individuals with Grade 1 NAFLD; and Group 4: 23 preobese or obese individuals with Grade 2–3 NAFLD. The demographic details of all participants were recorded. Abdominal ultrasonography and anthropometric assessments were conducted. Serum adropin and irisin levels were determined using the Enzyme-Linked Immunosorbent Assay (ELISA) method. Results: Serum adropin and irisin levels were lower in Groups 3 and 4 than in Group 1 (between the all groups; p = 0.006, p = 0.001, respectively), but were comparable between Groups 3 and 4. Furthermore, the atherogenic index of Group 1 was lower than that of Group 4 (p < 0.001). Among the hemogram parameters, the red cell distribution width value was higher in Group 3 than in Group 1 (p = 0.031). Conclusion: Serum adropin and irisin levels decrease in the presence of NAFLD, regardless of disease severity, which may play a role in the development and exacerbation of NAFLD in preobese and obese individuals.
- MakaleYalnız künye2025Investigation of Antioxidant and Enzyme Inhibitory Activities of Echinophora chrysantha: Microwave-Assisted Hydrodistillation and SPME Analysis of its Essential Oils
Şahin, Osman; Kanbolat, Şeyda; Çolak, Nevin Ulaş; Badem, Merve; Subaş, Tuğba; Şener, Sıla Özlem; Korkmaz, Nuriye; Kalaycıoğlu, Atila Taner; Öztürk, Ergün; Sayım, Alperen Ümit
Echinophora genus is represented by six species in Türkiye, including three endemics. The objective of this study was to ascertain the antioxidant, tyrosinase, and collagenase inhibitory activities and to analyse the volatile oil and extracts derived from E. chrysantha using GC/MS/SPME. The antioxidant and enzyme inhibition activities were evaluated using spectrophotometric methods. The findings indicated that the methanol extract of E. chrysantha exhibited the highest antioxidant activity. Furthermore, both the methanol extract and volatile oil of the plant demonstrated potent inhibitory effects on tyrosinase and collagenase. A total of 84 volatile compounds were identified from the hydrodistilled oil obtained using a microwave oven, and 23 compounds were detected by the solid-phase microextraction (SPME) method. The most frequently occurring volatile components were α-Phellandrene, o-Cymene, p-Cymene, E-β-Farnesene, Carotol, trans-Caryophyllene, and β-Phellandrene. The results of this study indicate that E. chrysantha has the potential to be developed for the treatment of hyperpigmentation issues and anti-aging applications. Furthermore, it may also have applications in pharmacology, cosmetics and the food industry.
- Derleme MakaleYalnız künye2025Design, spectroscopic characterization, in silico and in vitro cytotoxic activity assessment of newly synthesized thymol Schiff base derivatives
Başaran, Eyüp; Çakmak, Reşit; Sahin, Dicle; Köprü, Semiha; Türkmenoğlu, Burçin; Akkoc, Senem
Cancer is a global public health problem affecting millions of people every year. New anticancer drug candidates are needed to overcome the resistance to drugs used in the treatment of various types of cancer. In this study, two new series of benzenesulfonate-based thymol derivatives (14–19 and 20–25) were synthesized for the first time as promising chemotherapeutic agents and characterized using FT-IR, 1D NMR (1H- and 13C-NMR, APT, DEPT 135), 2D NMR (HETCOR and HMBC), and elemental analysis (CHNS). Antiproliferative activity of the molecules was determined against cancer cell lines, namely, the human lung adenocarcinoma cell line (A549) and the colorectal adenocarcinoma cell line (DLD-1), using MTT method for both 48 and 72 h. Compounds (14–25) showed cytotoxic activities against A549 with IC50 values ranging from 9.98 to 81.83 μM, respectively, compared to cisplatin (6.65 μM). These compounds exhibited antiproliferative activities against DLD-1 cancer cells at concentrations ranging from 4.29 to 53.62 μM, respectively, compared to cisplatin (9.91 μM). Especially, compound 16 displayed significant cytotoxicity on A549 and DLD-1 cancer cells with IC50 values of 9.98 and 10.75 μM, respectively. Finally, molecular docking studies were performed with Bcl-2, VEGFR-2, EGFR, and HER2 targets using the Schrödinger 2021-2 Maestro Glide program. The binding energy values and binding interactions of compounds 16 and 22 were determined to be the result of their interactions with these targets. Schrödinger 2021-2 Qikprop wizard drug similarity ratios and ADME prediction of all compounds 14–25 were also calculated.
- MakaleAçık Erişim2025Effects of Selective Serotonin Reuptake Inhibitor Treatment on Blood Pressure in Resistant Hypertensive Patients with Anxiety Disorder
Vatansever, Mehmet; Çakır, Osman Kağan
Objectives: This study aimed to evaluate the effects of selective serotonin reuptake inhibitor (SSRI) treatment on blood pressure (BP) control in resistant hypertensive patients with anxiety symptoms. Methods: This interventional study was conducted between January 2021 and March 2022 at a family medicine outpatient clinic. Patients with primary hypertension (HT), uncontrolled with monotherapy, and scoring ≥16 on the Beck anxiety inventory were included. All were switched to combination antihypertensive therapy. Those who accepted SSRI treatment constituted the case group, while those who declined formed the control group. Demographic and clinical characteristics were recorded. BP was measured at baseline and after 1 month. Results: Ninety-one patients were included, 30 (33.0%) male, the median age was 53.5 (35.0–68.0) years. Of the participants, 72 (79.1%) received combined therapy plus SSRI, while 19 (20.9%) received combined therapy only. After 1 month, systolic BP decreased by 15.0 (1.0–22.0) mmHg and diastolic BP by 13.0 (4.0–18.0) mmHg in the case group, compared to decreases of 8.0 (1.0–17.0) mmHg and 6.0 (2.0–13.0) mmHg in the control group (respectively, p<0.001 and p<0.001). Conclusion: SSRI therapy provided significantly greater reductions in both systolic and diastolic BP compared with combination antihypertensive treatment alone. Anxiety should be considered an important factor in resistant HT, and psychological as well as pharmacological interventions may enhance treatment outcomes.
- MakaleAçık Erişim2025Therapeutic Effects of tDCS on Calcium and Glutamate Excitotoxicity in a Cerebral Ischemia–Reperfusion Rat Model
Akcay, Guven; Demirdogen, Filiz; Kotan, Dilcan; Gul, Tuba; Yilmaz, Ali; Ergul, Yusuf Muslum; Celik, Cagla
AIM: To investigate the therapeutic effects of transcranial direct current stimulation (tDCS) on calcium and glutamate excitotoxicity caused by ischemia–reperfusion (IR). MATERIAL and METHODS: The IR model was generated by transient middle cerebral artery occlusion. tDCS treatment was applied at 1 mA for 30 min daily at the 2nd, 24th, and 48th h of IR. The motor and cognitive functions and the concentrations of Ca2+, glutamate, and N-methyl-D-aspartate receptor (NMDAR) in the hippocampus tissues were evaluated. RESULTS: Results showed a reduction in motor and cognitive functions in the IR group compared with that in the sham group, whereas these functions increased in the IR+tDCS group compared with those in the IR group. Ca2+, glutamate, and NMDAR concentrations were higher in the IR group than in the sham group but lower in the IR+tDCS group than in the IR group. CONCLUSION: These results suggest that tDCS treatment improves motor and cognitive dysfunctions after IR and exerts therapeutic effects on learning and memory through the regulation of Ca2+ and glutamate excitotoxicity.
- MakaleAçık Erişim2025Can digital leadership transform AI anxiety and attitude in nurses?
Tarsuslu, Sinan; Agaoglu, Ferhat Onur; Bas, Murat
Background: The lack of artificial intelligence applications in nursing education and the nursing profession in Turkey and the need for strategies for integrating artificial intelligence into the nursing profession continues. At this point, there is a need to transform the negative attitudes and anxiety that may occur in nurses. Objectives: It was aimed to reorganize the professional transformation in this parallel by analyzing the effect of digital leadership perception, which is explained as how nurses approach digital technologies and innovations and their awareness of how and with which methods they can use these technologies on artificial intelligence anxiety and attitude in the nursing profession. Design: The study was designed as descriptive, correlational, and cross-sectional. Participants: The research was conducted by reaching 439 nurses working in hospitals operating in three different regions of Turkey by simple random sampling method. Methods: In the first part of the data collection tool used in this study, digital leadership scale, artificial intelligence use anxiety, and artificial intelligence attitude scales were used, including questions determining the demographic information of nurses, their relationship with technology, artificial intelligence usage status and its importance in the profession. Results: It was determined that 29.8% of the nurses had a good relationship with technology, 66.3% knew about using artificial intelligence in health, and 27.3% wanted it to be more involved in their lives. It was determined that nurses' perceptions of digital leadership were at a medium level of 46.9% and a high level of 41.7%, 82.7% had a positive attitude towards artificial intelligence, and 82.7% had low or medium level anxiety when their artificial intelligence anxiety status was examined. There was a significant and negative relationship between digital leadership and AI anxiety (r = −0.434; p < 0.01), a significant and positive relationship between digital leadership and AI attitude (r = 0.468; p < 0.01), and a significant and negative relationship between AI attitude and AI anxiety (r = −0.629; p < 0.01). Finally, it was determined that nurses' perception of digital leadership indirectly affected AI anxiety through AI attitude (β = −0.230, 95% CI [−0.298, −0.165]). Conclusion: It is suggested that the anxiety and attitude towards artificial intelligence can be transformed positively with the effect of digital leadership, and in this parallel, the digital leadership phenomenon should be evaluated as a practical implementation strategy in integrating artificial intelligence into the nursing profession. Clinical Relevance: Our study showed that artificial intelligence attitude has a mediating role in the indirect effect of the perception of digital leadership in nursing on AI anxiety. It was determined that nurses' digital leadership perception, artificial intelligence anxiety, and artificial intelligence attitude differed significantly with demographic variables.
- MakaleAçık Erişim2025Structure-based inhibition of acetylcholinesterase and butyrylcholinesterase with 2-Aryl-6-carboxamide benzoxazole derivatives: synthesis, enzymatic assay, and in silico studies
Kuzu, Burak; Alagoz, M. Abdullah; Demir, Yeliz; Gulcin, Ilhami; Burmaoglu, Serdar; Algul, Oztekin
An important research topic is the discovery of multifunctional compounds targeting different disease-causing components. This research aimed to design and synthesize a series of 2-aryl-6-carboxamide benzoxazole derivatives that inhibit cholinesterases on both the peripheral anionic and catalytic anionic sides. Compounds (7–48) were prepared from 4-amino-3-hydroxybenzoic acid in three steps. The Ellman test, molecular docking with Maestro, and molecular dynamics simulation studies with Desmond were done (Schrodinger, 12.8.117). Compound 36, the most potent compound among the 42 new compounds synthesized, had an inhibitory concentration of IC50 12.62 nM for AChE and IC50 25.45 nM for BChE (whereas donepezil was 69.3 nM and 63.0 nM, respectively). Additionally, compound 36 had docking values of − 7.29 kcal/mol for AChE and − 6.71 kcal/mol for BChE (whereas donepezil was − 6.49 kcal/mol and − 5.057 kcal/mol, respectively). Furthermore, molecular dynamics simulations revealed that compound 36 is stable in the active gorges of both AChE (average RMSD: 1.98 Å) and BChE (average RMSD: 2.2 Å) (donepezil had average RMSD: 1.65 Å and 2.7 Å, respectively). The results show that compound 36 is a potent, selective, mixed-type dual inhibitor of both acetylcholinesterase and butyrylcholinesterase. It does this by binding to both the catalytically active and peripheral anionic sites of cholinesterases at the same time. These findings show that target compounds may be useful for establishing the structural basis for new anti-Alzheimer agents.
- MakaleYalnız künye2025Synthesis, characterization, cytotoxic activity, and molecular docking of 3,4-diaminobenzophenone-CuhNFs and its major components-based nanoflowers
Somturk-Yilmaz, Burcu; Altiparmak, Ayşegül; Turkmenoglu, Burçin; Akkoc, Senem
In this study, organic–inorganic hybrid nanoflower (hNFs) synthesis was realized using 3,4-diaminobenzophenone and Cu(II) metal ions as organic and inorganic ingredients, respectively. The characterization of the synthesized hNFs was carried out using SEM, EDX, FT-IR, XRD, and elemental mapping. The cytotoxic effect of hNFs was investigated against A549 (lung) and MCF-7 (breast) cancer cell lines. Interactions of 3,4-diaminobenzophenone were also investigated via molecular docking studies at the binding site of the human estrogen receptor (PDB ID:3ERT) and epidermal growth factor receptor tyrosine kinase (PDB ID: 1M17). Physicochemical and pharmacokinetic parameters for ligands that could be potential anticancer drug candidates were determined by ADME prediction calculations from in silico approaches. In vitro results showed an increase in cell death when the synthesized hybrid nanoflowers were applied against both cell lines, depending on the concentrations tested. Additionally, it was determined that the synthesized nanoflower was more effective on the MCF-7 cell line.
- MakaleAçık Erişim2025Antioxidant, and enzyme inhibition effects of chia (Salvia hispanica) seed oil: A comprehensive phytochemical screening using LC-HR/MS
Mutlu, Muzaffer; Bingol, Zeynebe; Ozden, Eda Mehtap; Köksal, Ekrem; Erturk, Adem; Goren, Ahmet C.; Alwasel, Saleh; Gulcin, İlhami
Background: In this study, the antioxidant and anti-Alzheimer's disease properties of Chia (Salvia hispanica) seed oil (CSO) were determined for the first time. Three different metal reduction and two different radical scavenging methods were used to determine the antioxidant properties of CSO. It exhibited higher antioxidant activity than vitamins E and C in the CUPRAC method. Results: CSO has shown excellent efficacy in the antioxidant methods used in this study. In the DPPH radical scavenging method, CSO exhibited higher radical scavenging potential than BHT, a standard and synthetic antioxidant. The anti-Alzheimer's disease properties of CSO were determined by inhibition of acetylcholinesterase enzyme and its IC50 value (17.60 µg/mL) was found to be close to the IC50 value of tacrine (8.82 µg/mL), the standard inhibitor of the enzyme. Inhibition properties of α-glycosidase enzyme and human carbonic anhydrase II isoenzymes were also studied. It was understood that CSO inhibited both enzymes at a lower rate than standard inhibitors. Also, the total phenolic and flavonoid contents of CSO were determined as 784.44 µg gallic acid equivalent (GAE)/mL oil and quercetin 150.00 µg QE)/mL oil, respectively. In addition, LC-HRMS chromatography application was performed to understand the phenolic content of CSO. It was determined that isosakuranetin (29.07 mg/L oil) was the most abundant polyphenolic compound in CSA. Also, seven polyphenolics of the studied remained below the detectable amount. Conclusions: It was found that CSO had effective antioxidant activity, polyphenolic contents and potent enzyme inhibition properties, associated with some global disease. How to cite: Mutlu M, Bingol Z, Ozden EM, et al. Antioxidant, and enzyme inhibition effects of chia (Salvia hispanica) seed oil: A comprehensive phytochemical screening using LC-HR/MS. Electron J Biotechnol 2025;74. https://doi.org/10.1016/j.ejbt.2024.12.002.
- MakaleAçık Erişim2025Comparative in vitro and in silico evaluation of the toxic effects of metformin and/or ascorbic acid, new treatment options in the treatment of Melasma
Yalçln, Hülya Tezel; Çaklr, Deniz Arca; Yirün, Anll; Sanajou, Sonia; Işlk, Gözde; Bozdemir, Özlem; Özçelik, Ibrahim; Güdül Bacanll, Merve; Zeybek, Naciye Dilara; Baydar, Terken
Melasma is a chronic condition that leads to the buildup of melanin pigment in the epidermis and dermis due to active melanocytes. Even though it is considered a non-life-threatening condition, pigment disorders have a negative impact on quality of life. Since melasma treatment is not sufficient and complicated, new treatment options are sought. Research on metformin and ascorbic acid suggested that they might be used against melasma in the scope of "drug repositioning."The MNT-1 human melanoma cell line was used to assess the effects of metformin, ascorbic acid, and metformin+ascorbic acid combination on cytotoxicity and oxidative stress. Melanin, cAMP, L-3,4-dihydroxyphenylalanine (L-DOPA) and tyrosinase levels were determined by commercial ELISA kits and tyrosinase gene expression was analyzed with RT-qPCR. Cytopathological evaluations were performed by phase contrast microscopy. Tyrosinase expression was determined by immunofluorescence (IF) staining of MNT-1 cells. The online service TargetNet was used for biological target screening. The parameters were not significantly altered by ascorbic acid applied at non-cytotoxic concentrations. On the contrary, metformin dramatically raised tyrosinase and intracellular ROS levels. Moreover, intracellular ROS levels and tyrosinase levels were found to be considerably elevated with the combined treatment. Also, potential metformin and ascorbic acid interactions were determined. According to the results, it can be said that these parameters were not significantly altered by ascorbic acid. On the contrary, metformin dramatically raised tyrosinase and intracellular oxidative stress levels. Moreover, intracellular oxidative stress and tyrosinase levels were elevated with the combined treatment. In conclusion, individual treatments of ascorbic acid or metformin may only provide a limited effect when treating melasma and extensive in vitro and in vivo research are required.
- MakaleYalnız künye2025Substituted bisbenzimidazole derivatives as multiple targeting agents to treat Alzheimer's disease, diabetes, and microbial infections
Algul, Oztekin; Mete, Burak; Turkmenoglu, Burcin; Saglamtas, Ruya; Alagoz, M.Abdullah; Dogen, Aylin; Gulcin, Ilhami; Burmaoglu, Serdar
This study presents the synthesis and bioactivity screening of a series of substituted bisbenzimidazoles (3a–l), assessed for their inhibitory effects on α-glycosidase, α-amylase, acetylcholinesterase (AChE), butyrylcholinesterase (BChE), as well as their antibacterial activities and metal chelation properties. Compound 3e exhibited the most significant inhibitory activity against intestinal α-glycosidase and α-amylase, showing IC50 values of 15.51 µM and 18.18 µM, respectively. All bisbenzimidazole derivatives demonstrated significant inhibitory activities, with Ki values between 0.99 and 2.98 nM for AChE and 0.40 to 2.18 nM for BChE. Antimicrobial analyses revealed significant antibacterial efficacy in compounds 3c and 3f, with IC50 values ranging from 10.75 to 12.83 μg/μL. This article presents a thorough evaluation of the pharmacological activities associated with bisbenzimidazole compounds 3a–l. To validate experimental results, selected compounds exhibiting notable enzyme inhibitory potential were subjected to molecular docking studies, which demonstrated their binding interactions within the active sites of target enzymes. Molecular dynamics simulation studies were carried out for 100 ns to determine the stability of the compounds in target proteins. During the simulation, it was observed that 3 h, 3 g, 3l, and 3e were stable in 4EY7, 4BDS, 3TOP, and 2QV4, respectively. Compounds 3 h, 3 g, 3e, and 3l have been identified as promising candidates for the inhibition of AChE, BChE, α-glycosidase, and α-amylase, respectively.
- MakaleAçık Erişim2025Intravesical BCG: A Double-Edged Sword? The Untold Story of Infection Risks
Barkay, Orçun; Keskin, Ercüment
Background and Objectives: Intravesical Bacillus Calmette-Guérin (BCG) therapy remains a cornerstone in the treatment of non-muscle-invasive bladder carcinoma due to its efficacy in reducing recurrence and progression rates. However, its use is associated with various complications—including urinary tract infections (UTIs)—which necessitates further exploration. This study aims to analyze UTIs occurring during intravesical BCG treatment, emphasizing the microbial spectrum, resistance patterns, and risk factors from an infectious diseases and clinical microbiology perspective. Materials and Methods: A retrospective analysis was conducted on 240 patients diagnosed with non-muscle-invasive bladder carcinoma who received intravesical BCG therapy between 2010 and 2021. Data were collected from hospital records, including demographic characteristics, comorbidities, number of intravesical BCG cycles, and microbiological findings. UTIs were classified based on susceptibility patterns, and statistical analyses were performed to determine associations between clinical variables and UTI risk. Results: UTIs developed in 39.1% (n = 94) of patients, with 25.8% (n = 62) caused by susceptible pathogens and 13.3% (n = 32) by resistant pathogens. The most common causative agent was Escherichia coli (80.7% in susceptible UTIs, 43.8% in resistant UTIs), followed by Pseudomonas aeruginosa and Klebsiella pneumoniae. The presence of diabetes mellitus and chronic kidney disease significantly increased the risk of developing a UTI (p < 0.05). A higher number of intravesical BCG cycles correlated with increased UTI occurrence (p < 0.001). Serum C-reactive protein (CRP) levels were significantly elevated in patients with resistant UTIs, while procalcitonin levels were not a reliable predictor of UTI occurrence. Conclusions: Intravesical BCG therapy is associated with a significant incidence of UTIs, particularly among patients with predisposing comorbidities. The increasing prevalence of antibiotic-resistant infections underscores the need for careful monitoring and targeted antimicrobial stewardship strategies. CRP may serve as a useful adjunctive marker for UTI diagnosis in this setting. Future studies should focus on optimizing infection control measures and refining diagnostic criteria to differentiate between BCG-related inflammation and infectious complications.
- MakaleAçık Erişim2025Myricetin protects against doxorubicin-induced acute kidney injury in rats by mitigating oxidative damage and apoptotic response
Karadogan, Muhammed Talha; Cicek, Betul; Cinisli, Kagan Tolga; Mendil, Ali Sefa; Ozkaraca, Mustafa; Yilmaz, Furkan; Suleyman, Halis
Introduction: Doxorubicin (DOX) is a potent anti-neoplastic agent widely preferred in treating various tumors. However, DOX’s off-target toxicity in healthy tissues, such as nephrotoxicity, limits its clinical utilization. DOX generates oxidative stress and apoptosis in the kidneys, which stimulates cytotoxic cellular signaling. Myricetin (MYC), an important natural flavonoid, exhibits antioxidant and antiapoptotic features. In this regard, the current report was designed to explore the renoprotective potential of MYC on DOX-induced nephrotoxicity. Methods: Animals were divided into four groups with six rats in each group: control, MYC, DOX, MYC + DOX. MYC was given orally to rats at 100 mg/kg for 10 days and DOX was injected intraperitoneally as a single dose of 20 mg/kg on the eighth day. Serum samples were evaluated for creatinine and blood urea nitrogen (BUN), and histopathological analysis of the kidneys was conducted. The levels of malondialdehyde (MDA), myeloperoxidase (MPO), total oxidant status (TOS), glutathione (GSH), glutathione peroxidase (GPx), and total antioxidant capacity (TAC) were measured in the renal tissues. Additionally, biochemical assessments of Bax and Bcl-2 proteins, along with immunohistochemical evaluations of the expression levels of caspase-3 and apoptosis-inducing factor (AIF), were conducted to evaluate apoptosis. Results: Pre-treatment of MYC decreased DOX-elicited elevation in creatinine and BUN levels (p < 0.05). Histopathological findings demonstrated the nephroprotective role of MYC on renal damage (p < 0.05), which was in harmony with the biochemical findings. Furthermore, MYC demonstrated antioxidant properties by reversing the increase in MDA, TOS, and MPO levels and the decrease in GSH, GPx, and TAS levels caused by DOX (p < 0.05). MYC pre-treatment also markedly prohibited DOX-induced elevation of Bax level, and rise of expression of caspase-3 and AIF, and reduction of Bcl-2 levels (p < 0.05). Conclusion: It could be supposed that the nephroprotective role of MYC towards DOX-induced kidney damage might be mediated by its antioxidant and antiapoptotic features.
- MakaleAçık Erişim2025The Relationship Between CRP Levels and Hospitalization, Discharge, Readmission and Mortality Rates of Geriatric Patients Presenting to the Emergency Department; [Acil Servise Başvuran Geriatrik Hastaların CRP Düzeyi ile Hastaneye Yatış, Taburculuk, Yeniden Başvuru, Mortalite Oranları Arasındaki İlişki]
Kantar, Yusuf; Baykan, Necmi; İpekten, Funda; İmamoğlu, Melih
Aim: reactive protein (CRP) levels and outcomes such as hospitalization, discharge, readmission, and mortality in geriatric patients (aged 65 and over) presenting to the emergency department for non-traumatic reasons. Material and Methods: CRP levels were retrospectively analyzed from blood tests taken during routine diagnostic and therapeutic processes for patients over 65 years of age who presented to the emergency department of a secondary state hospital for non-traumatic reasons between January 1, 2023, and June 30, 2023. Patients were categorized into five CRP level groups (5-50 mg/L, 51-100 mg/L, etc.) and evaluated for their medical history, complaints, hospitalization status, consultation requests, and mortality. Results: Patients with CRP levels between 5-100 mg/L were more frequently diagnosed with non-infective causes, while CRP levels over 101 mg/L were predominantly associated with infective causes. A statistically significant association was found between elevated CRP levels and increased rates of hospitalization and mortality (p < 0.001). Conclusion: A significant relationship was observed between CRP levels and the rates of consultation and hospitalization in geriatric patients admitted to the emergency department for non-traumatic reasons. Additionally, elevated CRP levels were strongly associated with increased mortality rates among hospitalized patients. The findings indicate that CRP levels exceeding 101 mg/L were associated with infectious diagnoses and poor outcomes, highlighting the need for close monitoring of these patients. CRP levels should be considered an integral part of the decision-making process in geriatric emergency patients.
- MakaleAçık Erişim2025Exploring the Nexus of Professional Commitment, Emotional Labor, and Self-Efficacy Among Community Pharmacists: Implications for Healthcare Delivery
Özkaya, Yağmur; Özçelikay, Gülbin
Objectives: This study aimed to explore the relationship between emotional labor, professional commitment, and self-efficacy among community pharmacists. Specifically, this study examined how self-efficacy influences professional commitment and the mediating role of emotional labor strategies in this relationship. Materials and Methods: A cross-sectional survey design was used to collect data from 396 community pharmacists. The study used a convenience sampling method and included standardized measures of emotional labor, professional commitment, and self-efficacy. Descriptive statistics were used to examine the levels of these variables among the participants. Multiple regression analyses were conducted to assess the interdependencies and mediating effects of emotional labor strategies. Results: General self-efficacy was positively correlated with emotional commitment (β=0.275, p<0.05) and continuance commitment (β=0.364, p<0.05), explaining 5% and 8% of their variances, respectively. A normative commitment was influenced by self-efficacy (β=0.464, p<0.05) and deep emotional labor (β=0.134, p<0.05), explaining 11% of its variance. Self-efficacy and deep emotional labor positively affected overall professional commitment (β=0.368, p<0.05), accounting for 15% of the variance. Conclusion: The results highlight the crucial role of self-efficacy in managing the emotional demands of the pharmacy profession and in fostering stronger professional commitment. Enhancing pharmacists’ self-efficacy and emotional management skills can improve their job satisfaction and commitment to the profession. These findings have clinical implications for the development of training interventions aimed at supporting pharmacists in coping with the emotional aspects of their work and improving their overall professional well-being.
- MakaleAçık Erişim2025Exploring chemical composition, anti-acetylcholinesterase, and anticancer activities of Pistacia atlantica Desf
Bakka, Chahrazad; Akşit, Huseyin; Zahnit, Wafa; Şahin, Bihter; Aydin, Ali; Tokul-Olmez, Ozge; Ozturk, Mehmet; Smara, Ouanissa; Dendougui, Hocine; Hadjadj, Mohamed
Context: The Atlas Pistachio (Pistacia atlantica Desf.) possesses numerous applications for therapeutic purposes. Objective: We set out to test various extracts from Pistacia atlantica Desf. fruits and leaves for their anticancer and acetylcholinesterase inhibitory effects. In addition to discovering the phytochemical profile of P. atlantica extracts responsible for the pharmacological effects that were researched. Materials and methods: The anti-acetylcholinesterase activity was performed against the acetylcholinesterase (AChE) enzyme. Anticancer assays were investigated by evaluating the antiproliferative and cytotoxicity potentials against five cancerous cell lines using the [3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MMT:Medical training therapy) assay. The phenolic profile was established against phenolic standards using high-performance liquid chromatography with diode-array detection (HPLC-DAD). Results: In anticholinesterase activity, the extracts of P. atlantica inhibited AChE activity in a dose-dependent manner. The stronger AChE inhibition activity was obtained for the n-BuOH extract of the fruits (IC50 47.80 ± 1.03 µg/mL) and compared to the leaf extracts. The results, compared with galantamine, showed a close inhibition activity. P. atlantica extracts also showed the most optimal antiproliferative and cytotoxic effect against cancer cell lines compared to positive control 5FU (Fluorouracil); the best antiproliferative activity was obtained for the fruit extracts compared to the leaf extracts. The spotted biological activities can be attributed to flavonoids and phenolic compounds in the extracts. The HPLC-DAD analysis identified the presence of 18 phytochemicals. The main compounds detected were luteolin, gallic acid, epicatechin, and protocatechuic acid. Discussion and conclusion: This study demonstrated moderate anticholinesterase and suitable antiproliferative activities of P. atlantica Desf. extracts, which opens up new possibilities for the pharmaceutical and food industries. This study suggests that the concentrations of phenolic compounds, along with the results of anticholinesterase and antiproliferative activities found in the fruit and leaf extracts of P. atlantica Desf, indicate this plant holds promise for pharmaceutical and food application industries.
- MakaleYalnız künye2025Chalcone-based imidazo[2,1-b]thiazole derivatives: synthesis, crystal structure, potent anticancer activity, and computational studies
Dadou, Said; Altay, Ahmet; Baydere, Cemile; Anouar, El Hassane; Türkmenoğlu, Burçin; Koudad, Mohammed; Dege, Necmi; Oussaid, Abdelouahad; Benchat, Noureddine; Karrouchi, Khalid
In this work, two novel chalcone-based imidazothiazole derivatives ITC-1 and ITC-2 were synthesized and characterized by 1H NMR, 13C NMR and high-resolution mass spectrometry with electrospray ionization, and chemical structure of ITC-1 was confirmed by single-crystal X-ray diffraction. Also, the anticancer activity of ITC-1 and ITC-2 was evaluated. First, antiproliferative activity tests were performed against cancer cells namely, human-derived breast adenocarcinoma (MCF-7), lung carcinoma (A-549), and colorectal adenocarcinoma (HT-29) cell lines, and mouse fibroblast healthy cell line (3T3-L1) by XTT assay. Afterward, mitochondrial membrane disruption (MMP), caspase activity, and apoptosis tests were performed on MCF-7 cells to elucidate the anticancer mechanism of action of the test compounds by flow cytometry analysis. XTT results revealed that both compounds exhibited a very high degree of antiproliferative effects on each tested cancer cell line with very low IC50 values while showing much lower antiproliferation on 3T3-L1 normal cells with much higher IC50 values. Besides, ITC-2 was determined to have a striking cytotoxic power competing with the chemotherapeutic drug carboplatin. Flow cytometry results demonstrated the mitochondrial-mediated apoptotic effects of both compounds through membrane disruption and multi-caspase activation in MCF-7 cells. Finally, molecular docking studies were performed to determine the structural understanding of the test compounds by their interactions on caspase-3 and DNA dodecamer enzymes, respectively. The interactions between the compound and the crystal structure were determined according to parameters such as free binding energies (ΔGBind), Glide score values, and determination of the active binding site. The obtained data suggest that ITC-1 and ITC-2 may be considered remarkable anticancer drug candidates. In addition to molecular docking via in silico approaches, the pharmacokinetic properties of compounds ITC-1 and ITC-2 were calculated using the Schrödinger 2021-2 Qikprop wizard. Communicated by Ramaswamy H. Sarma.
- MakaleAçık Erişim2025The Mediating Role of Career Stress and the Moderating Role of Psychological Resilience in the Relationship Between the Future Anxiety and the Psychological Well-Being of Prospective Social Studies Teachers
Bayram, Hüseyin; Pala, Şenol Mail
Prospective social studies teachers experience future anxiety and career stress in Turkey just as in different countries around the world. The aim of the study was to examine this issue. The mediating role of career stress and the moderating role of psychological resilience in the relationship between the future anxiety and psychological well-being of prospective social studies teachers were examined. A cross-sectional research model was used throughout the study. Maximum diversity sampling formed the participant group, which included 988 prospective social studies teachers from various universities in Turkey. The Future Anxiety Scale in University Students, the Psychological Well-Being Scale, the Korean Career Stress Inventory, and the Adult Resilience Measure provided the data used in the study. The data were analyzed using the SPSS 25 package program and the Process Macro 4.2 plug-in. It was concluded that future anxiety has a significant negative effect on psychological well-being. In addition, it was concluded that career stress has a mediating role and that psychological resilience has a moderating role in the relationship between future anxiety and psychological well-being. Suggestions led to an examination the hypotheses of this study with different experimental and longitudinal studies and with the participation of prospective social studies teachers who had graduated from university and could not be appointed.
- MakaleAçık Erişim2025Investigation of genetic causes in non-obstructive azoospermic patients
Dural, Bulut; Gecit, Ilhan; Aykanli, Emre; Ekici, Cemal; Oguz, Fatih; Koc, Ahmet
Background: Male factor infertility is a health problem that affects millions of couples around the world. Male factor infertility is responsible for approximately more than half of all cases of infertility. About 15% of men and 10% of women with infertility may have genetic abnormalities, including chromosomal abnormalities and single gene mutations. In this study, results of genetic analys is of the infertile male patients who underwent testicular sperm extraction (TESE) with the diagnosis of non-obstructive azoospermia were evaluated in order to reveal genetic defects that impair or prevent spermatogenesis in male infertility. Methods: We compared the results of peripheral blood chromosome analysis, molecular karyotyping, male infertility genetic panel, and also testosterone, prolactin, follicular stimulating hormone and luteinizing hormone levels in non-obstructive azoospermic infertile patients aged 26–44 years, and investigated the relationship between these parameters and genetic mutations. Results: As a result of this research, among 26 patients, INSL3 (insulin-like peptide 3) gene mutation, which is considered pathogenic according to the criteria published by the American College of Medical Genetics and Genomics (ACMG) was detected in 1, FSHR (follicle stimulating hormone receptor) gene polymorphism in 17, CFTR (cystic fibrosis transmembrane conductance regulator) mutations in 5, CATSPER1 (cation channel sperm associated 1) and TEX101 (testis expressed 101) in 1, LHCGR (luteinizing hormone/choriogonadotropin receptor) in 1, ZMYND15 (zinc finger mynd-type containing 15) in 1, DNAH5 (dynein axonemal heavy chain 5) in 2, and DNAH11 (dynein axonemal heavy chain 11) changes in 1 patient. In the chromosome analysis, 47XXY Klinefelter syndrome was observed in 6 patients. Conclusions: The results have shown that non-obstructivea zoospermic patients with complaints of infertility may have other genetic abnormalities leading to infertility, despite the results of chromosomal analysis of the peripheral blood samples were within normal reference limits. Investigating these underlying genetic disorders helped us find the cause of infertility in ourpatient population.
