SDG 3 · Sağlık ve Kaliteli Yaşam · SDG · Erzincan Binali Yıldırım Üniversitesi

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ArticleYalnız künye2026/r/ebyu/11163
Asymmetric benzazole derivatives as dual antibiofilm and anti-wall teichoic acid agents against Staphylococcus aureus
Buga Aktekin, Mine; Oksuz, Zehra; Alagoz, Mehmet Abdullah; Uluca Han, Bahar; Algul, Oztekin
Aims: Antibiotic-resistant bacteria, particularly methicillin-resistant Staphylococcus aureus (MRSA), have renewed interest in antivirulence strategies. This study aimed to design, synthesize, and evaluate asymmetric di‑heterocyclic benzazole derivatives as antibiofilm agents targeting wall teichoic acid (WTA) biosynthesis. Materials and methods: Twenty‑one novel bis‑heterocyclic benzazole derivatives bearing an ethyl‑thio linker were synthesized and characterized. Antibacterial activity was determined by broth microdilution. Antibiofilm activity (inhibition and eradication) was assessed at sub‑minimal inhibitory concentrations (sub‑MICs) using crystal violet. Molecular docking and 200 ns molecular dynamics (MD) simulations were performed against key WTA enzymes (TarGH, TarS, TarM, TarL, and TarJ). Results: Compounds 18, 21, 23, and 24 showed potent antibacterial activity against methicillin‑susceptible S. aureus (MSSA) and MRSA, with MIC values as low as 15.62 µg/mL. All selected compounds significantly inhibited biofilm at sub‑MIC levels. Compound 24 was most effective, with an MBIC50 of 3.90 µg/mL against MSSA and eradicated pre‑formed biofilms at 7.81 µg/mL. Docking and MD simulations revealed stable interactions of compounds 23 and 24 with TarGH and TarS, suggesting a WTA‑targeting mechanism. Conclusions: Di‑heterocyclic benzazole derivatives, especially compound 24, are promising scaffolds for antibiofilm agents against S. aureus. Our findings support further mechanistic and preclinical evaluation as potential antivirulence therapeutics.
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ArticleYayıncıda açık erişim2026/r/ebyu/11155
Association between postoperative arterial blood gas parameters and cognitive dysfunction following coronary artery bypass grafting: a prospective observational study
Demir, Özge; Üstündağ, Hilal; Onk, Oruç Alper
Background: Postoperative cognitive dysfunction (POCD) is a significant complication of coronary artery bypass grafting (CABG), with impaired quality of life and long-term outcomes. The association between postoperative arterial blood gas (ABG) abnormalities and early cognitive outcomes remains unclear. Objectives: To investigate the association between perioperative ABG parameters and SMMSE-assessed cognitive function in patients undergoing elective CABG. Methods: This prospective study of 41 CABG patients recorded SMMSE scores and ABG parameters (pH, PO₂, PCO₂, HCO₃⁻, SO₂, base excess) preoperatively and 3–7 days postoperatively; POCD was defined as a ≥ 2-point SMMSE decline. Analyses included correlation, regression with VIF diagnostics, logistic regression, ROC analysis, and FDR correction. Results: Age was 63.15 ± 7.42 years; 29 of 41 patients (70.7%) met POCD criteria, with SMMSE declining (p < 0.001). Postoperative pH, PO₂, SO₂, HCO₃⁻, and base excess correlated with SMMSE change (ρ = 0.567–0.608, all p < 0.001) and differed between groups (all p ≤ 0.003); PCO₂ showed neither association. In multivariable regression, the model was significant (adjusted R² = 0.332, p = 0.002), but no individual parameter remained significant, reflecting multicollinearity (VIF up to 5.21); these also predicted POCD by logistic regression, and ROC analysis identified HCO₃⁻ as the strongest discriminator (AUC = 0.885), followed by pH (0.866) and base excess (0.851). Conclusions: Postoperative ABG derangements, metabolic acidosis and hypoxemia, are significantly associated with early cognitive decline after CABG. No single parameter was an independent predictor, reflecting interrelation; pH, HCO₃⁻, and base excess showed the strongest discriminative accuracy. These observational associations warrant confirmation in interventional studies.
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ArticleAçık Erişim2026/r/ebyu/11136
Morphometric analysis and typing of talus and calcaneus bones from the Eastern Anatolian population
Karakas, Asli Beril; Akbulut, Yalcin; Yarar, Burhan; Sunar, Mukadder
BACKGROUND: The talus and calcaneus are critical components of foot biomechanics, contributing to stability and movement. Despite their importance, data on their morphometric and articular characteristics in the Eastern Anatolian population are scarce. This study aimed to address this gap by analyzing the morphometry and facet configurations of the talus and calcaneus in this region and highlighting their clinical and biomechanical significance. MATERIALS AND METHODS: A total of 32 tali and 29 calcanei were analyzed using high-resolution computed tomography (CT) and 3D reconstruction. Morphometric measurements, including lengths, widths, and facet areas, were performed, and articular facet types were classified based on established criteria. Comparative analyses were conducted with data from other populations. RESULTS: Side-specific variations were observed in lengths and sulcus dimensions. Type B was the most prevalent facet configuration in both the talus and calcaneus, with unique patterns such as Type B3 emphasizing the anatomical distinctiveness of the Eastern Anatolian population. Comparisons with other populations revealed larger morphometric dimensions and distinct facet distributions, underscoring population-specific differences. CONCLUSIONS: This study provides valuable morphometric and facet classification data for the talus and calcaneus in the Eastern Anatolian population. These findings contribute to a deeper understanding of regional anatomical variations and have significant implications for clinical applications, forensic investigations, and prosthetic design.
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ArticleAçık Erişim2026/r/ebyu/11134
Isovanillin-derived bis-hydrazones as dual cholinesterase and carbonic anhydrase inhibitors: synthesis, enzymatic profiling, and computational insights from molecular docking and dynamics
Türkeş, Cüneyt; Yapar, Gönül; Duran, Hatice Esra; Lolak, Nebih; Akocak, Suleyman
Aims: To develop isovanillin-based bis-hydrazones as multitarget inhibitors of acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and human carbonic anhydrase I/II (hCA I/II). Materials & methods: Twelve bis-hydrazones (4a–4l) were synthesized in two steps and evaluated by spectrophotometric enzyme assays, Lineweaver–Burk kinetics, molecular docking, MM-GBSA, molecular dynamics simulations, and in silico ADME/Tox profiling. Results: All compounds showed nanomolar inhibition. Compound 4d was the most potent AChE/BChE inhibitor (KI = 10.46 and 3.56 nM), while 4a and 4j led the hCA I/II panel (KI = 3.46 and 16.12 nM). Docking, MM-GBSA, and molecular dynamics supported dual-site cholinesterase engagement and non-zinc, peripherally anchored hCA inhibition. Conclusions: Isovanillin-based bis-hydrazones, particularly 4d, 4a, and 4j, represent promising multitarget leads for cholinergic and hCA-linked disorders.
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Review ArticleAçık Erişim2026/r/ebyu/11121
Emergency radiology in displaced populations: imaging and practical challenges
Çakmak, Vefa; Çakmak, Pınar; Herek, Duygu; Aydın, Sonay
Migrants and refugees tend to use emergency departments as their primary source of care, leading to a substantial increase in emergency radiological imaging. Migrants and refugees have risk factors, such as low vaccination rates, poor hygiene, malnutrition, and inadequate self-care, which increase their risk of contracting infectious diseases. For example, the prevalence of tuberculosis among refugee populations in host countries is increasing, and imaging findings related to tuberculosis are frequently observed by radiologists. Strengthening screening programs in host countries for tuberculosis and other infectious diseases among migrant populations can help mitigate the risk of transmission within migrant communities. Another condition, cystic echinococcosis, is more common among refugees and migrants from the Middle East and Afghanistan. For radiologists working in host countries, echinococcosis involving the liver and lungs should be considered in the differential diagnosis. Both intentional (e.g., violence, assault) and unintentional (e.g., workplace injuries, accidents) traumas are frequently encountered in emergency radiology, particularly among refugees and immigrants. Workplace injuries are four times more common among migrants and refugees than among the local population due to their work in high-risk industries, such as construction and heavy industry, and emergency radiology frequently encounters radiological findings of organ injuries due to falls from height. In addition, healthcare professionals in emergency radiology face various challenges when dealing with migrant and refugee patients, such as communication barriers, social security problems, and psychological distress. Copyright @ 2026 Author(s)
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ArticleYalnız künye2026/r/ebyu/11113
Measuring food insecurity in Türkiye: validation of the four domain food insecurity scale (4D-FIS)
Memiç-İnan, Cansu; Başpınar, Büşra; İmrol, Fatih
The aim was to conduct the Turkish validity and reliability study of the Four-Domain Food Insecurity Scale (4D-FIS). In this cross-sectional study, confirmatory factor analysis (CFA) was used to test the validity of the 4D-FIS scale. The 4D-FIS scale consists of four domains: quantitative, qualitative, psychological, and social. The Cronbach-α of the 4D-FIS scale was 0.84, indicating that the scale was reliable. CFA confirmed the four-factor structure of the 4D-FIS, with fit indices indicating good model fit. The Turkish 4D-FIS scale was found to be valid and reliable for evaluating food security in the quantitative, qualitative, psychological, and social domains.
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ArticleYalnız künye2026/r/ebyu/11105
Comparative evaluation of the effects of bisphenol A and/or bisphenol F on oxidative stress and cytokine responses in SH-SY5Y neuroblastoma cells: in silico and in vitro
Özçelik, İbrahim; Çakir, Deniz Arca; Yildiztekin, Kübra Gizem; Erkekoğlu, Pınar
Bisphenol A (BPA) is a well-known endocrine-disrupting chemical, whereas bisphenol F (BPF), a common BPA substitute, is less characterized, particularly regarding neuronal-derived cells and combined BPA + BPF exposure. Human SH-SY5Y neuroblastoma cells were exposed to BPA, BPF, or BPA + BPF for 24 h. Cell viability was assessed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), and oxidative stress and cytokine endpoints were evaluated at IC20 concentrations. SwissTargetPrediction and molecular docking were used to examine potential interactions with estrogen receptor alpha (ERα), estrogen receptor beta (ERβ), and androgen receptor (AR). BPA was more cytotoxic than BPF, with IC50 values of 173.2 and 456.8 µM, respectively; the BPA + BPF combination IC50 was 196.9 µM. BPA and BPA + BPF significantly increased reactive oxygen species by 15.74% and 18.32% and protein carbonyl levels by 227.69% and 172.67%, respectively. BPA markedly increased interleukin-6, whereas BPF strongly increased tumor necrosis factor-alpha. In contrast, TNF-α decreased in the BPA and BPA + BPF groups, while malondialdehyde showed a non-significant downward trend. Docking predicted interactions of both compounds with AR, ERα, and ERβ, with binding energies of −7.3 to −8.5 kcal/mol. BPA and BPF produced distinct cytotoxic, oxidative, and cytokine-response profiles after acute exposure. BPA-containing treatments were associated with greater oxidative stress-related changes, whereas BPF elicited a distinct inflammatory response. Predicted receptor interactions require functional validation, and further studies should assess chronic, low-dose, and mixed exposures.
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ArticleAçık Erişim2026/r/ebyu/11080
Evaluation of the Efficacy of Acarbose on Ovarian Microbiota Profile, Ovary–Gut Axis, and SAP (1–10) Gene Expression in Rats Administered With Candida albicans Exosomes
Dabanlioglu, Bulent; Celebi, Demet; Celebi, Ozgur; Baser, Sumeyye; Guler, Mustafa Can; Tanyeli, Ayhan; Ari, Betul; Kalayci, Sıla Ektas; Fakirullahoglu, Mesud; Kiliclioglu, Metin
Candida albicans is a commensal fungus of the vaginal and reproductive tract microbiota, but its overgrowth contributes to mucosal infections and reproductive dysfunctions. The fungus secretes exosomes carrying virulence factors, including secreted aspartyl proteinase (SAP) genes, which are critical for tissue invasion and immune modulation. Acarbose, an α-glucosidase inhibitor, has been shown to suppress C. albicans biofilm formation and hyphal transition. This study is aimed at evaluating the effects of acarbose on the ovarian microbiota, gut–ovary axis, and SAP gene expression profile in a rat model following exposure to C. albicans exosomes. Rats were divided into two groups: the control group received intraperitoneal C. albicans exosomes (8 log10 CFU/mL), whereas the acarbose group received the same exosomes followed by oral acarbose (25 mg/kg/day). Exosomes were characterized by NTA and SEM. Ovarian tissue gene expression (SAP1–10) was analyzed by qRT-PCR. Inflammatory cytokines and tight junction proteins were assessed via ELISA, and microbiota composition was determined using 16S rRNA sequencing. Acarbose significantly reduced IL-8 and TNF-α levels while increasing IL-10, ZO-1, claudin-5, and occludin expression compared with Candida-infected controls (p < 0.05). Gut microbiota diversity and classification success were higher in the acarbose group, indicating microbial balance restoration. Acarbose mitigated C. albicans exosome-induced inflammation and barrier dysfunction while enhancing microbial diversity, suggesting its potential role in modulating the ovarian–gut axis and reducing fungal virulence through SAP gene suppression.
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ArticleAçık Erişim2026/r/ebyu/11063
Predictors of procedural intervention in otolaryngology consultations: a retrospective multivariable analysis of 1,366 cases
Altıparmak, Serkan; Kars, Ayhan
OBJECTIVE: The objective of this study is to evaluate the demographic and clinical characteristics of cases consulted with the ear, nose, and throat department in a tertiary hospital, the consultation indications, and independent factors associated with the need for procedural intervention. METHODS: This retrospective single-center study included patients undergoing ear, nose, and throat consultation. Demographics, consultation characteristics, indications, imaging, procedures, and hospitalization were recorded. Procedural intervention rates and independent factors associated with intervention requirements were also examined. RESULTS: Consultations for 1,366 patients were analyzed. The great majority of consultations (76.5%) originated from the emergency department, and procedural interventions were performed in approximately half the cases (48.8%). The most common reasons for consultation were suspected foreign bodies (n=248, 18.2%), nasal fracture (n=231, 16.9%), and epistaxis (n=132, 9.7%). The highest procedural intervention requirements were in the soft tissue trauma group (n=118; 90.7%) and in the epistaxis (87.9%) and nasal fracture (78.3%) cases. Consultation indication was the strongest independent predictor. Epistaxis, trauma-related consultations, and suspected foreign bodies were significantly associated with the need for procedural intervention. CONCLUSION: Consultation indication was the main determinant of intervention. Epistaxis, trauma-related conditions, and suspected foreign bodies were significantly independently associated with intervention requirements. Early identification of these high-risk indications may assist in the optimization of ear, nose, and throat emergency resource planning and consultation triage.
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ArticleAçık Erişim2026/r/ebyu/11060
Earthquake safety culture scale development study
Tercan, Bahadır; Çelik, İbrahim Halil; Yılmaz, Gürkan; Usta, Galip
Earthquakes have been one of the most significant natural hazards threatening humanity throughout history. In this study, we aimed to develop an earthquake safety culture scale to psychometrically measure individuals' attitudes and behaviors regarding their personal life safety before and after an earthquake. During the scale development phase, two different surveys were administered for exploratory factor analysis (EFA) and confirmatory factor analysis (CFA). The study population consisted of individuals aged 18 and over living in Türkiye. 445 Individuals participated in the EFA and 309 in the CFA. The earthquake safety culture scale, a two-factor structure ("pre-earthquake" and "during and after earthquake"), yielded highly reliable and valid results. It is believed that the resulting scale will contribute to disaster managers in the process of building an earthquake-resilient society, as well as to local and national disaster management policies such as training, awareness, planning, and exercises for community-supported disaster management. It is recommended that this developed scale be retested in different cultures and its relationship with various variables that may influence "pre-earthquake" and "during and after earthquake" attitudes regarding disaster management be investigated.
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ArticleYalnız künye2026/r/ebyu/11052
Evaluation of the Learning Curve of Freehand Transperineal Prostate Biopsy Performed Under Local Anesthesia
Fakir, Ali Emre; Bitkin, Alper; Ekşi, Mithat; Akdağ, Azad; Karadağ, Mehmet; Koşargelir, Ahmet; Karadağ, Serdar; Polat, Hakan; Kargı, Taner; Taşçı, Ali İhsan
Background: To evaluate the learning curve (LC) of office-based freehand transperineal prostate biopsy (f-TPBx) performed under local anesthesia and its correlation with the operator experience. Methods: A total of 176 patients who underwent f-TPBx between June and November 2023 were retrospectively analyzed. The first 44 cases of four different physicians (2 consultant urologists and 2 urology residents) were included, and patients were stratified into quartiles (Q1–Q4). The LC was assessed based on differences in procedure time, fibromuscular core rates, cancer detection rates, and complication rates across quartiles. A multivariate analysis was performed to identify factors affecting procedure time. LC of consultant urologists and urology residents was compared. Results: There was a significant reduction in procedure duration across quartiles (Q1: 22.3 ± 4.2 minutes versus Q4: 13.7 ± 4.1 minutes; P < .001), alongside an increase in clinical significant prostate cancer detection rates (P = .034). Fibromuscular core rates significantly decreased, and overall complication rates declined over time (both P = .001). It has been observed that the LC for consultant urologists was completed after approximately 20 cases. Cumulative SUM and locally weighted scatterplot smoothing analyses demonstrated that consultant urologists reached the plateau phase earlier. According to multivariate regression analysis, quartile variable were among the factors significantly affecting procedure time (P < .001). Conclusions: The LC for f-TPBx appears to plateau after approximately 20 cases, suggesting that stable procedural performance can be achieved within a relatively short period. The technique provides notable advantages, including high patient comfort, a strong safety profile, and rapid learnability, which support its successful integration into clinical practice.
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ArticleAçık Erişim2026/r/ebyu/11037
Direct Relationship Between Heparin Binding to Midkine and Pleiotrophin and the Development of Acute Deep Vein Thrombosis
Aydin, Suna; Polat, İsmail; Tural, Kevser; Duger, Nurullah; Ugur, Kader; Sahin, İbrahim; Aydin, Suleyman; Lee, Do-Youn
Background/Objectives: The underlying molecular mechanisms of deep vein thrombosis (DVT), which continues to be a major global public health concern, remain unclear. A key component of anticoagulant therapy, heparin (HP) interacts with heparin-binding growth factors including pleiotrophin (PTN) and midkine (MK), both of which have basic amino acid-rich domains that have a strong affinity for HP. The purpose of this study was to determine if changes in the levels of circulating HP, MK, and PTN are linked to the onset of acute DVT. Methods: Thirty patients diagnosed with acute DVT by venous Doppler ultrasonography (VDU) and 28 healthy controls with normal VDU findings were enrolled. Serum HP, MK, and PTN concentrations were measured using ELISA. In DVT patients, blood samples were obtained before and after routine subcutaneous low-molecular-weight heparin treatment; controls provided a single blood sample. ROC curve analysis was used to assess diagnostic performance. Results: Prior to treatment, patients with acute DVT exhibited significantly lower serum HP levels (p < 0.05) and significantly higher MK and PTN levels compared with healthy controls (both p < 0.05). Following heparin administration, serum HP levels increased significantly (p < 0.05), while MK and PTN levels showed a decreasing trend that did not reach statistical significance (p > 0.05). ROC curve analysis demonstrated limited diagnostic performance for HP (sensitivity 10.3%, specificity 68.8%), PTN (62.1%, 54.2%), and MK (82.8%, 35.4%). Conclusions: Decreased circulating HP and increased MK and PTN levels are characteristics of acute DVT that may indicate endogenous HP sequestration through binding to these growth factors. This imbalance could lead to less free HP being available, which would encourage the formation of thrombus. Therapeutic approaches that target MK- and PTN-mediated HP interactions may constitute a unique approach for the therapy of acute DVT, as evidenced by the partial normalization seen after exogenous heparin delivery.
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ArticleAçık Erişim2026/r/ebyu/11030
Therapeutic Effects of Cichorium intybus L. Extract in an Experimentally Induced Insulin Resistance Model in Rats
Ermis, Uğur; Gül, Hüseyin Fatih; Gözcü, Sefa; Özdemir, Bengül; Hamdemir, Özgecan; Binnetoğlu, Damla
Introduction: Insulin resistance (IR) is a central metabolic feature of polycystic ovary syndrome (PCOS). Althou gh metformin improves insulin sensitivity, its adverse effects limit long-term use, prompting interest in herbal alternatives. The present study aimed to investigate the effects of Cichorium intybus L. ethanolic extract on PCOS-associated IR in a letrozole-induced rat model, compared with metformin. Materials and Methods: A total of twenty-four female Sprague–Dawley rats were allocated into four groups: healthy control (H), PCOS-associated insulin resistance (IR), metformin-treated (MET), and Cichorium intybus ethanolic extract–treated (C.I). PCOS was induced by oral letrozole administration (1 mg/kg/day) for 21 days. Following confirmation of IR by estrous cycle disruption and oral gl ucose tolerance test (OGTT), treatments were administered orally for 15 da ys. Metabolic parameters, serum insulin, glucose, lipid profile, sex hormones, and HOMA-IR index were evaluated. Skeletal muscle tissues were examined histopathologically and by immunohistochemical analysis of insulin receptor (INSR), insulin receptor subsrate 1 (IRS1), and insulin receptor subsrate 2 (IRS2) expression. Results: PCOS-associated IR rats showed impaired glucose tolerance, hyperinsulinemia, increased HOMA-IR, dyslipidemia, and hormonal imbalance. C. intybus ethanolic extract treatment significantly improved glucose tolerance, insulin levels, HOMA-IR, and lipid profile, with effects comparable to metformin. While metformin markedly restored INSR, IRS1, and IRS2 expression in skeletal muscle, C. intybus produced only moderate improvements. Conclusion: C. intybus ethanolic extract improved metabolic, hormonal, and biochemical paramete rs in PCOS-associated IR. However, its effects on skeletal muscle insulin signaling markers were less pronounced than metformin, suggesting a complementary therapeu tic role mainly through systemic metabolic regulation.
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ArticleYalnız künye2026/r/ebyu/11024
Novel Imino Derivatives of Thymol: Synthesis, in vitro Cytotoxic Activities, Molecular Docking Analysis and ADME Prediction
Başaran, Eyüp; Türkmenoğlu, Burçin; Akkoc, Senem
Cancer is a significant global health challenge that affects millions of individuals every year. The development of new anticancer drugs is imperative to address the issue of drug resistance in cancer therapy. Therefore, in this study, novel imino derivatives of thymol (3a-c) were synthesized and evaluated as anticancer agents. These compounds were structurally elucidated using elemental analysis and various spectroscopic methods, including 1H and 13C Nuclear Magnetic Resonance (NMR) and Fourier Transform Infrared Spectroscopy (FT-IR). The cytotoxic activities of the new compounds on human liver cancer (HepG2) and healthy human kidney (HEK-293T) cell lines were investigated. Compounds were tested at various concentrations to determine their IC50 values. Among the tested new compounds, 3a and 3b demonstrated cytotoxicity in HepG2, with IC50 values of 88.97 µM and 106.10 µM, respectively. Furthermore, compound 3a also exhibited significant toxicity against the HEK-293T cell line, suggesting that further modifications are needed to improve selectivity for healthy cells. ADME properties analysis resulted in the detection of two lead compounds (3a and 3b) among compounds 2a-c and 3a-c. For the most active compounds 3a and 3b, molecular docking study on epidermal growth factor tyrosine kinase receptor (PDB ID: 3POZ), PI3K alpha (PDB ID: 4FA6), c-kit tyrosine kinase (PDB ID:1T46) was performed to verify the in vitro experimental results.
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ArticleYayıncıda açık erişim2026/r/ebyu/11018
Evaluation of gossypetin’s effects on gut microbiota profile and TLR4, Myd88, NFKB, and NLRP3 signaling pathways in rats
Akkas, Onder; Celebi, Demet; Celebi, Ozgur; Baser, Sumeyye; Kalayci, Sıla Ektas; Ari, Betül; Guler, Mustafa Can; Dabanlıoglu, Bulent; Celebi, Selahattin; Kılıclıoglu, Metin
Gut microbiota plays a crucial role in maintaining host homeostasis by regulating metabolic processes and immune responses. Disruptions in microbial composition are closely associated with inflammatory diseases and are often linked to the activation of key signaling pathways such as Toll-like receptor 4/myeloid differentiation primary response 88/nuclear factor kappa TLR4/MyD88/NF-κB and NLR family pyrin domain-containing 3 (NLRP3) inflammasome. Natural bioactive compounds, particularly flavonoids, have gained attention due to their potential to modulate both gut microbiota and inflammation-related pathways. In this context, the present study aimed to evaluate the effects of gossypetin on gut microbiota composition and its regulatory role on TLR4, MyD88, NF-κB, and NLRP3 signaling pathways in a rat model. Adult female Wistar albino rats were divided into control and gossypetin-treated groups (50 mg/kg, oral gavage/56 days dose). Gut microbiota was analyzed by 16S rRNA sequencing, and protein expression levels were assessed using Western blot. Histopathological, immunohistochemical, and immunofluorescence analyses were also in liver, intestinal, and spleen tissue performed. Gossypetin administration reduced microbial diversity and altered microbiota composition, with increases in Mediterraneibacter spp., Blautia spp., and Lactobacillus spp. Western blot results showed significant decreases in NLRP3 (p ≤ 0.01) and NF-κB (p ≤ 0.05) levels, while TLR4 and MyD88 remained unchanged. Histological analyses revealed mild tissue alterations and increased oxidative stress markers. These results suggest that gossypetin modulates microbiota composition and exerts selective anti-inflammatory effects, highlighting its potential in microbiota-associated inflammatory regulation.
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ArticleAçık Erişim2026/r/ebyu/10992
A Case of Dose-Dependent Lamotrigine-Induced Priapism in a Patient with Bipolar Disorder; [Bipolar Bozukluğu Olan Bir Hastada Lamotrijin ile İlişkili Doza Bağlı Priapizm: Olgu Sunumu]
Akkuş, Mustafa
Introduction: Priapism is defined as a prolonged and persistent erection of the penis without sexual stimulation. Priapism can be caused either by organic factors or as a side effect of drugs. Mood stabilizer induced priapism has been reported less frequently compared to other psychotropic agents. This paper reports a case of lamotrigine induced priapism. Case: A 24-year-old patient had a diagnosis of bipolar disorder for 4 years. He had been treated with lamotrigine (200 mg/day), sertraline (25 mg/day) and risperidone (2 mg/day) for last year. He developed priapism one week after the lamotrigine dose was increased to 300 mg/day during a depressive episode. Priapism improved the day after the lamotrigine dose was reduced to 250 mg/day. Conclusion: Epilepsy patients with lamotrigine-induced sexual dysfunction or priapism have been reported previously. In this case, a patient diagnosed with bipolar disorder developed dose dependent lamotrigine-induced priapism. In clinical practice, the possible risk of priapism should be taken into consideration in patients using lamotrigine, especially during dose increases.
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ArticleAçık Erişim2026/r/ebyu/10988
The Needs of Parents Caring for Children with Epilepsy Questionnaire (NPCEQ-TR): Turkish adaptation, validity, and reliability study
Kasımoğlu, Necla; Aydın, Muhammet Ali; Tuncay, Birgül; Gürol, Ayşe; Adıyaman, Abdullah
Objectives: This study aimed to translate the Needs of Parents Caring for Children with Epilepsy Questionnaire (NPCEQ) into Turkish (NPCEQ-TR) and evaluate its psychometric properties, including validity and reliability, within a Turkish cultural context. Methods: A cross-sectional and methodological design was employed. Data were collected online from 310 parents of children with epilepsy in 2025. The adaptation process followed international guidelines and included translation, back-translation, content validation, and pilot testing. Construct validity was examined through confirmatory factor analysis (CFA). Internal consistency was assessed using Cronbach’s alpha and McDonald’s omega coefficients. Results: The content validity indices were satisfactory (I-CVI = 0.80– 1.00; S-CVI = 0.91). The KMO value (0.952) and Bartlett’s test (χ² = 5173.501, p < .001) confirmed that the data were suitable for factor analysis. CFA supported the original five-factor structure, with fit indices indicating an excellent model fit (χ²/df = 1.27; CFI = 0.976; TLI = 0.974; RMSEA = 0.030). Cronbach’s alpha and McDonald’s omega coefficients ranged from 0.770 to 0.930 for the subscales, and were 0.958 and 0.950, respectively, for the total scale. Conclusion: The NPCEQ-TR demonstrated strong construct validity, high internal consistency, and cultural relevance. It is a reliable and practical instrument for assessing the multidimensional needs of parents caring for children with epilepsy, and it can be utilized effectively in family-centered nursing and psychosocial interventions in Turkey.
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ArticleYalnız künye2026/r/ebyu/10963
Alpha-pinene mitigates renal ischemia–reperfusion injury via anti-oxidant, anti-inflammatory, anti-apoptotic effects and favorable eNOS/iNOS shifts in rats
Sebin, Saime Ozbek; Gulakar, Basak; Tanyeli, Ayhan; Guler, Mustafa Can; Erbas, Elif; Baser, Sumeyye; Celebi, Demet
Acute kidney damage frequently results from renal ischemia–reperfusion (RI/R). Alpha-Pinene (AP), a plant-derived monoterpene, has antioxidant, anti-inflammatory, and anti-apoptotic properties. We investigated whether AP limits early kidney damage after experimental RI/R. Female Wistar rats (n = 7 per group) were assigned to Sham, RI/R, RI/R + AP 50 mg/kg, or RI/R + AP 100 mg/kg. After 45 min of clamping both renal pedicles, a 24-h reperfusion period ensued. AP (i.p. immediately before reperfusion) was administered immediately before reperfusion. ELISA for inflammatory cytokines (IL-1β, TNF-α, IL-10), oxidative stress markers (MDA, IMA, SOD), and nitric oxide synthase isoforms (iNOS, eNOS). Histopathology (hematoxylin/Mallory's trichrome staining) for structural kidney injury scoring. Immunohistochemistry for apoptosis/autophagy markers (Bcl-2, cleaved caspase-3, LC3B) and NF-κB p65. In kidney homogenates, RI/R increased IL-1β, TNF-α, MDA, IMA, and iNOS and reduced IL-10, SOD, and eNOS compared to Sham. AP counteracted these alterations in a manner proportional to the dose; the 100 mg/kg dose most consistently approached Sham values. Histology showed diffuse vascular congestion/hemorrhage, tubular degeneration, and widening of Bowman's space after RI/R—all ameliorated by AP, with milder lesions at 100 mg/kg. Immunohistochemistry demonstrated lower cleaved caspase-3, LC3B, and NF-κB p65 (trend) with AP, alongside higher Bcl-2, supporting anti-apoptotic and anti-autophagic effects. A single intraperitoneal dose of AP administered immediately before reperfusion mitigated early renal injury after RI/R by improving redox balance, dampening inflammatory signaling, shifting the eNOS/iNOS profile toward endothelial protection, and reducing structural damage. AP merits further study as a potential adjunct to limit I/R–related kidney injury, including longer follow-up with functional renal endpoints and expanded dosing/timing strategies.
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ArticleYayıncıda açık erişim2026/r/ebyu/10955
18β-Glycyrrhetinic acid-loaded silver nanoparticles mitigate neuroinflammation and endoplasmic reticulum stress in the brain tissue of diabetic rats
Parlak, Seçil Nazife; Yakut, Seda; Kara, Adem; Demir, Özlem; Şebin, Saime Özbek
Objective(s): Diabetes mellitus (DM) causes oxidative stress, neuroinflammation, and endoplasmic reticulum (ER) dysfunction that contribute to neurodegeneration. This study investigated the effects of 18β-glycyrrhetinic acid-loaded silver nanoparticles (18β-GA-AgNPs) on brain injury in diabetic rats. Materials and Methods: Fifty-six male Wistar rats were divided into eight groups: Sham, 18β-GA, AgNPs, 18β-GA-AgNPs, DM, DM+18β-GA, DM+AgNPs, and DM+18β-GA-AgNPs. Diabetes was induced by alloxan (120 mg/kg, IP), and treatments were administered orally for 14 days. Biochemical markers (MDA, GSH, SOD), histopathology, and expression of ER stress and apoptotic proteins (ATF6, IRE1, Caspase-3, BCL-2, CREB, TNF-α, and IL-1β) were evaluated. Results: The DM group exhibited significant increases in MDA, TNF-α, IL-1β, ATF6, and Caspase-3 with reduced GSH, SOD, and BCL-2, indicating oxidative stress, inflammation, apoptosis, and ER stress. In contrast, IRE1 levels remained unchanged in DM rats but showed a slight elevation in the AgNPs group. Treatment with 18β-GA-AgNPs markedly reduced MDA, TNF-α, IL-1β, ATF6, and Caspase-3, while restoring GSH, SOD, BCL-2, and CREB expression. Histopathological analysis confirmed neuronal apoptosis and perivascular and extracellular space enlargement in DM rats, whereas 18β-GA-AgNPs substantially attenuated these changes. Overall, 18β-GA-AgNPs provided synergistic neuroprotection by suppressing oxidative stress, inflammation, and ER stress while enhancing antioxidant and anti-apoptotic defenses. Conclusion: These findings suggest that 18β-GA-AgNPs may represent a promising therapeutic strategy against diabetes-associated neurodegeneration, although further long-term, ultrastructural, and sex-inclusive studies are warranted.
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ArticleYayıncıda açık erişim2026/r/ebyu/10928
Optical Coherence Tomography Angiography–Based Evaluation of Foveal Avascular Zone and Macular Vessel Density in Prediabetic Patients
Utlu, Bahadır; Utlu, Elif Sedanur; Çinici, Emine; Akgöz, Hasan; Bayrakçeken, Kemal; Kozan, Betül Dertsiz
Background: To assess macular layer thickness, macular vessel density, and foveal avascular zone (FAZ) parameters in prediabetic patients compared with healthy normoglycemic controls using optical coherence tomography angiography (OCTA). Methods: Thirty prediabetic patients (group A; fasting plasma glucose 100-125 mg/dL, postprandial plasma glucose 140-199 mg/dL, glycated hemoglobin 5.7%-6.4%) and 30 age-matched normoglycemic subjects (group B) were included. The OCTA imaging was used to evaluate superficial capillary plexus (SCP) and deep capillary plexus (DCP) vessel densities, as well as FAZ area and perimeter. Participants with poor image quality, high refractive error, glaucoma, prior intraocular surgery, chorioretinal atrophy, or other ocular/sys-temic comorbidities were excluded. Results: The SCP and DCP perfusion densities (PDs) were lower in the prediabetic group, with significant reductions in the inferior and temporal quadrants of the DCP and the temporal quadrant of the SCP (P <.05). The FAZ area and perimeter were larger in the prediabetic group but not statistically significant (P >.05). Macular thickness was greater in all quadrants in group A, with significant thickening in the nasal and inferior quadrants (P <.05). Conclusion: Prediabetic patients demonstrated early microvascular and structural changes, including reduced macular PD, increased macular thickness, and FAZ enlargement. These findings suggest subclinical retinal involvement in prediabetes, warranting larger longitudinal studies.
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