SDG 3 · Sağlık ve Kaliteli Yaşam · SDG · Erzincan Binali Yıldırım Üniversitesi

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MakaleYalnız künye2024/r/ebyu/12769
Diagnostic Value of GSTP1, RASSF1, and RASSF2 Methylation in Serum of Prostate Cancer Patients
Aykanli, Emre; Arisan, Serdar; Arisan, Elif Damla; Yavuzsan, Abdullah Hizir
Purpose: Considering the inadequacy of PSA measurement in the diagnosis of prostate cancer, it is aimed to establish a potential liquid biopsy diagnostic panel. Materials and Methods: 39 patients who underwent TRUS-biopsy and 15 healthy volunteers were included. Approximately 15 ml of venous blood samples taken from healthy volunteers and patients before biopsy were separated as plasma. Hypermethylation status of GSTP1 and RASSF1:RASSF2 genes was revealed in cfDNA materials collected from plasma samples. Correlation of this epigenetic change detected in PCa, BPH and healthy volunteer groups with pathology results was examined. Results: Pathology reports of 39 patients included were 13 PCa, 3 ASAP, 3 HGPIN, and 20 BPH. In total, 3 of the patients with PCa had positive GSTP1, 4 had RASSF1 and 9 had positive RASSF2 methylation. It was seen that RASSF2 had the highest sensitivity (69%), specificity (39%) and NPV (80%), while RASSF1 had the highest PPV (30%). When the binary combinations of genes were examined it was observed that the GSTP1:RASSF1 combination had the highest sensitivity (46%), specificity (76%) and NPV (82%). When the methylation of all three genes was examined, it was observed that the sensitivity was quite low (8%), but the specificity (83%) increased significantly. Conclusion: Although we observed that the GSTP1 and RASSF1 methylation positivity rates that we examined in our study were higher in patients without prostate cancer, we found that the RASSF2 methylation rate was higher in patients with prostate cancer. randomized controlled studies are needed.
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MakaleAçık Erişim2024/r/ebyu/12767
Importance of parental anxiety in management of developmental breast conditions in children A study with a prospective hierarchical regression model
Özdamar, Mustafa Yaşar; Biçer, Şenol
The parent is the most critical link and decision-maker between the patient and the healthcare provider in treating many pediatric diseases. This entity is essential for the management of pediatric breast diseases for which the rate of surgical intervention is known to be very low. Although previous publications have emphasized that pediatric breast diseases may cause alarming anxiety in parents, the demographic factors that influence this anxiety have not been investigated. Even if practitioners complete patient management with appropriate procedures, treatment is incomplete if the questions remain unanswered. In this observational prospective study, we investigated the demographic factors that affect parental anxiety, which should be prioritized to prevent incomplete management. The Beck Anxiety Inventory score (BAS) created by the parents of 409 boys and girls aged 0 to 17 with breast conditions was recorded at the diagnosis, termination of treatment, and final control stages. A 2-stage hierarchical logistic regression model was applied to show how strongly the demographic characteristics of parents and their children predicted the parental BAS. Of the demographic characteristics, there was a significant correlation (P < .05) between the patient’s sex, age, developmental period, Tanner stage, referral status, management method, family’s place of residence, economic distress, and BAS. However, according to the 2-stage hierarchical regression model, only 3 demographic characteristics, the patient’s gender, place of residence, and method used in patient management, significantly predicted BAS (P < .05, ΔR2 = .35). Among the many factors that affect anxiety experienced by parents whose son or daughter has breast problems, the gender of the child, place of residence of the family, and management methods used by the practitioner are demographic characteristics that should be taken into consideration.
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MakaleYalnız künye2024/r/ebyu/12762
Syringic acid guards against indomethacin-induced gastric ulcer by alleviating inflammation, oxidative stress and apoptosis
Ferah Okkay, Irmak; Okkay, Ufuk; Cicek, Betul; Karatas, Ozhan; Yilmaz, Aysegul; Yesilyurt, Fatma; Hacimuftuoglu, Ahmet
The purpose of this study was to evaluate the effects of syringic acid, an anti-oxidant, on indomethacin induced gastric ulcers in rats. Experimental groups were control, ulcer, ulcer treated with 20 mg/kg esomeprazole (a proton pump inhibitor that reduces acid secretion), and ulcer treated with 100 mg/kg syringic acid. Rats were pretreated with esomeprazole or syringic acid two weeks before ulcer induction. Our histopathological observations showed that either syringic acid or esomeprazole attenuated the severity of gastric mucosal damage. Moreover, syringic acid and esomeprazole pretreatments alleviated indomethacin-induced damage by regulating oxidative stress, inflammatory response, the level of transforming growth factor-β (TGF-β), expressions of COX and prostaglandin E2, cell proliferation, apoptosis and regulation of the NF-κB signaling pathway. We conclude that either esomeprazole or syringic acid administration protected the gastric mucosa from harmful effects of indomethacin. Syringic acid might, therefore be a potential therapeutic agent for preventing and treating indomethacin-induced gastric damage.
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MakaleAçık Erişim2024/r/ebyu/12759
Mean S1 inlet and outlet view angles are not safe for all individuals according to three-dimensional tomographic measurements
Tanoğlu, Oğuzhan; Gökgöz, Mehmet Burak; Subaşı, İzzet Özay; Arıcan, Gökhun; Koçkara, Nizamettin
Although there are many studies evaluating optimal inlet and outlet angles required for the correct placement of S1 iliosacral screws, there is no study evaluating reliability and feasibility of these angles for all individuals on three-dimensional (3D) anatomical models. A total of 100 women and 100 men were selected randomly. A vertical line was created according to long axis of the tomography device on which patient was lying in supine position. The automatized best-fit planes were created on superior and inferior endplates, anterior cortex including notch region and posterior cortex of first sacral vertebrae using 3D imaging software to measure mean inlet and outlet angles. We observed no statistically significant difference between gender groups in terms of inlet and outlet angles. Mean inlet view is obtained for anterior cortex of S1 in 22.5 ± 9.5° and for posterior cortex in 46.5 ± 9.3°. Mean fluoroscopic view angle of S1 for superior outlet is 40.3 ± 7.6 and for inferior outlet is 46.9 ± 8.8. Mean anterior and posterior S1 inlet view angles do not accurately visualize anterior cortex of 74 (37%) and posterior cortex of 66 (33%) individuals. Mean superior and inferior S1 outlet view angles do not accurately visualize superior endplate of 74 (37%) and inferior endplate of 56 (28%) individuals. Due to individual alterations of spatial position of sacrum, mean inlet and outlet view angles of S1 are not sufficient to visualize the iliosacral screws under fluoroscopy in many individuals.
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DüzeltmeAçık Erişim2024/r/ebyu/12758
Correction to: Beyond expectations: safinamide’s unprecedented neuroprotective impact on acute spinal cord injury (European Journal of Trauma and Emergency Surgery, (2024), 10.1007/s00068-024-02513-y)
Gökten, Murat; Zırh, Selim; Sezer, Can; Bahador Zırh, Elham; Bulut Gökten, Dilara
The original version of this article, published on 11 April 2024, unfortunately contained a mistake. The following Authors and their affiliations were missing from the author list. Selim Zırh Department of Histology, Binali Yıldırım University, Erzincan, Turkey Can Sezer Department of Neurosurgery, University of Health Sciences, Adana City Training and Research Hospital, Adana, Turkey Elham Bahador Zirh Department of Histology, TOBB University of Economics and Technology, Ankara, Turkey Dilara Bulut Gökten Department of Internal Medicine-Rheumatology, Tekirdag Namik Kemal University, Tekirdag, Turkey The publisher apologizes for the error. The original article has been corrected.
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MakaleYalnız künye2024/r/ebyu/12756
The Protective Effect of Gallic Acid Against Bisphenol A-Induced Ovarian Toxicity and Endocrine Disruption in Female Rats
Gezer, Arzu; Üstündag, Hilal; Kılıç Baygutalp, Nurcan; Erbaş, Elif; Özkaraca, Mustafa
Purpose: This study aimed to investigate the protective effects of gallic acid (GA) against ovarian damage induced by bisphenol A (BPA) exposure in female rats. We evaluated whether GA can mitigate the adverse effects of BPA on ovarian structure, inflammatory markers, oxidative stress, apoptosis, and reproductive hormone levels. Methods: Thirty-two female rats were categorized into four groups: control, GA, BPA, and GA+BPA. Histopathological evaluations of ovarian tissue were performed using hematoxylin-eosin staining. The immunohistochemical analysis was conducted for inflammatory, oxidative DNA damage, and apoptotic markers (Tumor necrosis factor alpha [TNFa], cyclooxygenase-2 [COX2], interleukin-1 beta [IL-1b], 8-hydroxydeoxyguanosine [8-OHdG], and caspase 3). Oxidative stress was assessed by measuring malondialdehyde and superoxide dismutase levels. Furthermore, follicle-stimulating hormone (FSH), luteinizing hormone (LH), estrogen, and progesterone levels were quantified using enzyme-linked immunosorbent assay. Results: Histopathological outcomes revealed that BPA significantly induced follicular degeneration, which was effectively mitigated by GA treatment (P < 0.05). Immunohistochemical analysis highlighted the exacerbation of inflammatory responses and oxidative DNA damage and apoptosis (TNFa, COX-2, IL-1b, 8-OHdG, and caspase 3) in BPA-exposed tissues, which were reduced in the presence of GA (P < 0.05). The assessment of oxidative stress demonstrated that GA could significantly decrease lipid peroxidation and partially restore antioxidant defense mechanisms disrupted by BPA (P < 0.05). Hormonal profiling indicated that BPA exposure altered the levels of FSH, LH, estrogen, and progesterone, with GA treatment showing a capacity to modulate these changes, especially in progesterone levels (P < 0.05). Conclusions: The findings suggest that GA exhibits protective properties against BPA-induced ovarian damage through its antioxidative and anti-inflammatory activities, alongside its ability to modulate hormonal imbalances. This research underscores the therapeutic potential of GA in safeguarding reproductive health against environmental toxicants.
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MakaleAçık Erişim2024/r/ebyu/12751
EFFECT OF TAXIFOLIN ON DOXORUBICIN-INDUCED OXIDATIVE CARDIAC DAMAGE IN RATS: A BIOCHEMICAL AND HISTOPATHOLOGICAL EVALUATION
Aldemir, M.N.; Kara, A.V.; Mammadov, R.; Yazici, G.N.; Cicek, B.; Yavuzer, B.; Coskun, R.; Sakin, A.; Gulaboglu, M.; Suleyman, H.
Doxorubicin is a widely used anthracycline-derived broad-spectrum antitumoral antibiotic drug. However, cardiotoxicity due to doxorubicin treatment has warranted dose reduction or complete discontinuation in certain cases. The role of oxidative stress in the pathogenesis of doxorubicin-induced cardiotoxicity has been previously demonstrated. Against this background, this study aimed to investigate the protective effect of the potent antioxidant flavone taxifolin against possible oxidative heart damage biochemically and histopathologically induced by doxorubicin. Albino Wistar male rats were divided into three groups: healthy controls (HG), a group given doxorubicin alone (DG), and a group given taxifolin + doxorubicin (TDG). Taxifolin was administered orally at a dose of 50 mg/kg via gavage. Doxorubicin was injected intraperitoneally at a dose of 5 mg/kg. This procedure was repeated for 7 days. The results of the biochemical experiment showed that taxifolin significantly inhibited doxorubicin-induced malondialdehyde increases and glutathion decreases in heart tissues. In addition, taxifolin significantly suppressed the increases in cardiac damage markers, such as serum troponin I, creatine kinase, and creatine kinase-MB, induced by doxorubicin. Taxifolin treatment has also been histopathologically shown to alleviate doxorubicin-induced heart tissue damage. Accordingly, the results of the present study suggest that taxifolin may be useful in the treatment of doxorubicin-induced oxidative heart damage.
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MakaleAçık Erişim2024/r/ebyu/12745
Two new compounds from endemic Centaurea paphlagonica (Bornm.) Wagenitz and their cytotoxic activities
Tüfekçi, Ali Rıza; Demirtaş, İbrahim; Akşit, Hüseyin; Arslan, Şevki; Kocabıyık, Kübra; Zeybek, Sibel; Ozen, Tevfik; Köksal, Ekrem
Centaurea paphlagonica (Bornm.) Wagenitz is an endemic plant in Turkey. Pyrocatechol, vanillic acid, 3,4-dihydroxy benzoic acid, 5-O-caffeoylshikimic acid, tamarixetin, chlorogenic acid methyl ester, quercetin, 1,3-dicaffeoylquinic acid, tamarixetin-7-O-β-D-glucopyranoside, quercimetrin, daucosterin, paphlagonicanin B, tamarixetin-7-O-β-rutinoside, rutin, chlorogenic acid, isoorientin, orientin, 3-O-feruloylquinic acid, quercetagetin-3-methyl ether 6-O-β-glucopyranoside, diosmetin 6-C-β-glucopyranoside, quercetagetin 4′-methyl ether 7-O-β-glucopyranoside, paphlagonicanin A, nepetin, cirsiliol, desacylcynaropicrin, and 8α-O-(2′,3′-dihydroxyisobutyryl) desacylcynaropicrin were isolated from both flower and aerial parts of C. paphlagonica. These compounds were identified using 1D and 2D NMR methods and ESI-MS. The MTT assay assessed the antiproliferative activities of all isolated (known and new compounds) compounds on Caco-2, LNCaP, A549, HeLa, and HEK-293 cell lines. The 8α-O-(2′,3′-dihydroxyisobutyryl) desacylcynaropicrin demonstrated the highest activity against CaCo-2 and HeLa cancer cell lines.
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MakaleAçık Erişim2024/r/ebyu/12743
The relationship between serum adropin levels and erectile dysfunction
Keskin, Ercument; Ekici, Ozgur; Erdogan, Abdullah; Bozkurt, Aliseydi; Sambel, Murat; Karabakan, Mehmet; Mertoglu, Cuma
Erectile dysfunction (ED) is associated with endothelial damage, especially atheroscle-rosis. The search for biomarkers that can predict ED still continues. Adropin is known to affect nitric oxide (NO) bioavailability and energy homeostasis. In our study, we have aimed to investigate the relationship between serum adropin levels and ED. Male patients with and without ED between 40–60 years of age, who presented to the outpatient clinics of urology between November 2019–February 2020, were prospectively included in the study. Biochemical values measured at the time of admission to the outpatient clinic. According to the International Index of Erectile Function-5 (IIEF-5) scores which range between 5 and 25 points, patients with a score ≤21 were considered to have ED. The patients were divided into two groups: as ED and non-ED control groups. Laboratory values obtained at admission to outpatient clinics of urology were compared between groups. Patients with (n: 40), and without (n: 40) ED were included in the study. The mean age (50.2 ± 5.7 years), average body mass index (BMI) (29.7 ± 2.5 kg/m2 ), IIEF score (15.8 ± 6 pts), serum adropin (584.8 ± 172 pg/mL), and total testosterone (396.4 ± 91.7 ng/dL) levels were recorded. Serum adropin and testosterone levels were statistically significantly higher in the non-ED group than in the ED group (712.3 ± 222 pg/mL vs. 511.1 ± 145 pg/mL, p < 0.001 and 420.5 ± 56 ng/dL vs. 374.3 ± 98 ng/dL, respectively p = 0.032). Whereas fasting blood glucose (FBG) values were found to be statistically significantly higher in the ED group (100.2 ± 14 mg/dL vs. 143.8 ± 78 mg/dL, p = 0.001). According to the results of our study, serum levels of adropin which improves endothelial functions were comparatively lower in ED patients, as expected.
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MakaleAçık Erişim2024/r/ebyu/12742
Correlation of Diffusion weighted MR Imaging and ADC Values of Hepatic Metastasis of Gastrointestinal Stromal and Gastroenteropancreatic Neuroendocrine Tumors
Aydın, Hasan; Metin, Melike Ruşen; Kızılgöz, Volkan
Background: DWI and ADC-mapping was performed to analyze hepatic metastasis of GIST, GEP-NET. Objective: The objective of this study is to present hepatic metastasis of GIST and GEP-NET with Diffusion weighted MR imaging(DWI) and the Apparent diffusion coefficients (ADC) values of masses. Methods: 18 GIST patients and 8 GEP-NET patients were examined retrospectively. 11 males and 6 females were present in GIST group, 7 males to 5 females were involved in GEP-NET group. 18 primary GIST and 10 hepatic metastasis of GIST, 8 original GEP-NET and 19 hepatic metastasis of GEP-NET; total 55 GIST and GEP-NET masses were analysed by ADC mapping. MR images were acquired by 1,5 T MR units (32 mT/min gradient strength-Achieva; Philips Healthcare, Best, Netherlands and 32 channel GE Signa GE-Wisconsin-USA); by using a 4-8 channel standard phased-array torso XL coil, all images were evaluated by an Abdominal MRI experienced radiologist. DWI was performed in the transverse plane by using spin-echo-planar imaging sequence. Results: No statistical differences were observed between GIST and GEP-NET patients according to age and gender variations. No significant statistical differences were observed according to the diameters and ADC values of GIST and GEP-NET patients. A significant statistical difference was observed between GIST and GEP-NET groups in terms of size of liver metastasis which was significantly higher in GIST patients. All three groups (GIST_Hep. MET, GEP-NET_Liver_Met and normal) were statistically differed according to ADC values. With the ROC curve analysis: Hepatic metastasis of GIST(n=10) and normal liver (n:47) had cut-off value for ADC: 0.925 under AUC: 0.939 with regard to ADC values and regarded 89.4% Sensitivity, 100% Specificity, 100% PPV and 66.7% PPV. ROC curve of GEP NET_ Hepatic metastasis (n=19) group and normal liver (n:47) group presented cut-off value for ADC: 0.860 under AUC: 0.967 correlated to ADC values with 93.6% sensitivity, 89.5% specificity, 95.7% PPV and 85% PPV. Conclusion: High cellular tumors resulted from liver metastasis of GIST and GEP-NET’s, and a positive correlation was observed between ADC values and cellularity/differentiation ratios of metastatic masses.
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MakaleYalnız künye2024/r/ebyu/12740
Liquid chromatography–tandem mass spectrometry profile and antioxidant, antimicrobial, antiproliferative, and enzyme activities of Thymus pectinatus and Thymus convolutus: in vitro and in silico approach
Akman, Tuğrul Çağrı; Şimşek, Samed; Akşit, Zeynep; Akşit, Hüseyin; Aydin, Ali; Tüfekçi, Ali Riza; Adem, Sevki; Yilmaz, Mustafa Abdullah
BACKGROUND: The objective of this study is to investigate the antiproliferative, antioxidant, antimicrobial, and enzyme activity capacities and phytochemical compositions of Thymus pectinatus (TP), Thymus convolutus (TC), which are endemic to Türkiye. Quantitative analysis of phenolic compounds in the extracts was conducted using liquid chromatography–tandem mass spectrometry, targeting 53 phenolic compounds. RESULTS: Rosmarinic acid, quinic acid, and cynaroside were identified as the major compounds, exhibiting quantitative variation in both extracts. The extracts had a high total phenolic content, with 113.57 ± 0.58 mg gallic acid equivalents (GAE)/g extract for TP and 130.52 ± 1.05 mg GAE/g extract for TC. Furthermore, although both extracts exhibited high total flavonoid content; the TP extract (75.12 ± 1.65 mg quercitin equivalents (QE)/g extract) displayed a higher flavonoid content than the TC extract (30.24 ± 0.74 mg QE/g extract) did. The extracts had a promising antiproliferative effect on C6, HeLa, and HT29 cancer cell lines with a less cytotoxic effect (10.5–14.2%) against normal cells. Both extracts exhibited very potent inhibitory activity against the xanthine oxidase enzyme, with half-maximal inhibitory concentration values of respectively 2.07 ± 0.03 μg mL−1 and 2.76 ± 0.06 μg mL−1 and moderate activity against tyrosinase and α-glucosidase. Docking simulations proved that rosmarinic acid and cynaroside, the major components of the extracts, were the most potent inhibitors of xanthine oxidase. According to antimicrobial activity results, the TC extract exhibited moderate activity against Staphylococcus aureus, and the TP extract had strong activity against both Enterococcus faecium and S. aureus. CONCLUSION: These findings emphasize the beneficial effects of the two endemic Thymus species on human health and suggest their potential use as plant-derived bioactive agents.
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MakaleAçık Erişim2024/r/ebyu/12737
Essential oils combat Botrytis cinerea in apples: unveiling sugar, vitamin, and antioxidant dynamics
Karakus, Sinem; Atıcı, Okkes; Turan, Metin; Kaya, Ozkan
This study investigated the effects of individual and combination applications of EOCs against Botrytis Cinerea in apple fruits, with a focus on sugar, vitamin, and antioxidant enzyme dynamics. In protective and curative treatments, sucrose content varied between 18.9 and 87.9 g.L-1, with the “CT” group exhibiting significantly higher sucrose levels (87.9 g.L-1). Glucose content ranged from 6.2 g.L-1 (“CT” and “C + F”) to 35.5 g.L-1 (“T + C + E + F”). Fructose content varied significantly, with the “F + T + C + E” treatment showing the highest (11.4 g.L-1) and the “CT” group the lowest (2.5 g.L-1). Rhamnose content in the curative treatment reached 14.9 g.L-1 (“F + T”) and in the protective treatment, the “C + E + F” group had the highest (3.3 g.L-1). Galactose content ranged from 0.1 to 13.0 g.L-1. Xylose content in curative treatment ranged from 12.1 to 13.6 g.L-1, with the combination “F + T” displaying the highest. In preventive treatment, arabinose content ranged from 0.7 to 3.0 g.L-1, with the “T + C + F” group having the highest. In terms of vitamins, the combination “T + F” showed the highest B1 (25.4 mg.100 g-1) and B2 (24.6 mg.100 g-1) content. Vitamin A, B1, and B2 increased in the “F + T” group, and vitamin C increased in the “F + T + C” group (33.4 mg.100 g-1). The quantification of oxidative stress markers and antioxidant enzymes revealed significant reductions in H2O2, MDA, and proline levels in EOC applications. In preventive treatment, the combination “T + C + E + F” exhibited the most effective improvement in SOD activity (556.5 EU mg-1), CAT activity increased significantly in the “T + C + F” group (189.3 EU mg-1), and APX activity saw the highest increase in the “T + C + E + F” group (185.6 EU mg-1). Contrastingly, curative treatments showed higher H2O2, MDA, and proline levels. The most significant decrease in H2O2 level occurred in the “T + C + E + F” group (36.7 nmol.g-1), and a significant decrease in MDA level was noted in the same group. Notable changes were observed in the activities of antioxidant enzymes, with the “F + T + C + E” group displaying the highest SOD activity (440.7 EU mg-1), and the “F + T” group showing the highest GST activity (542.0 EU mg-1). This research provided comprehensive insights into the biochemical responses of apple fruits to EOC treatments, offering potential strategies for effective fungal management in agriculture.
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MakaleAçık Erişim2024/r/ebyu/12736
IN VITRO AND IN SILICO STUDIES ON LIGNAN SECOISOLARICIRESINOL DIGLUCOSIDE; [LİGNAN SEKOİZOLARİSİRESİNOL DİGLUKOSİT ÜZERİNE İN VİTRO VE İN SİLİKO ÇALIŞMALAR]
Bayar, İrem; Çağlar Yavuz, Sevtap; Akkoç, Senem
Objective: Lignans are important biologically active compounds in diphenolic structure. Secoisolariciresinol diglucoside (SDG) is a significant type of lignan known to have anti-cancer properties. This study aimed to investigate the antiproliferative activity properties of SDG on hepatocellular carcinoma cells (HepG2), colorectal cancer cells (DLD-1), lung carcinoma (A549), and prostate cancer (PC3) cell lines. Material and Method: Cell viability of cancer cells was determined by the MTT method after treatment with various concentrations of SDG at 48 or 72 hours. The DFT (Density Functional Theory) analysis of the SDG was performed using Spartan'10 and visualized. Drug-likeness and absorption, distribution, metabolism, excretion, and toxicity (ADME-Tox) properties of this compound were examined. Molecular docking was carried out to research the biological activity of SDG. Result and Discussion: Our results showed that SDG exhibited significant cytotoxicity only against DLD-1 cells with IC50 value of 37.45 µM, but inactive against other cancer cell lines as in vitro. 4UYA, which biomarker for colon cancer, is the crystal structure of the MLK4 kinase domain. The binding energy value for the SDG-MLK4 kinase domain was calculated as -6.1 kcal/mol. Anticancer potential was verified by in vitro assay and in silico molecular docking study. In conclusion, this study revealed the protective aspect of SDG against colon cancer and showed that it has promising anticancer activity.
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MakaleYalnız künye2024/r/ebyu/12735
Determining the Mental Status, Sleep Quality, and Eating Behaviors of University Students During the COVID-19 Pandemic: A Cross-Sectional Study
Şahin, Hilal; Çebi, Kadir; Yıldırım, Arzu; Hacıhasanoğlu Aşılar, Rabia
BACKGROUND: The COVID-19 pandemic has resulted in adverse effects on individuals’ physical and mental health worldwide, with university students also being adversely affected by this process. OBJECTIVES: This research aimed to determine the mental condition, sleep quality, and eating behaviors of university students during the COVID-19 process. METHOD: This cross-sectional study was conducted with 1,065 university students. Study data were collected with an online questionnaire including a personal information form, the Depression Anxiety Stress Scale, the Pittsburgh Sleep Quality Index, and the Dutch Eating Behavior Scale. RESULTS: Students’ poor sleep quality was determined to be 69.5%. It was also determined that students’ depression and stress levels were moderate that their anxiety levels were low, their eating behaviors were generally problematic, and more than half had poor sleep quality. In addition, eating disorders and poor sleep quality increased as depression, anxiety, and stress levels increased, and eating disorders increased as poor sleep quality increased (p <.05). CONCLUSIONS: The results emphasize the importance of the strengthening of students’ mental health, particularly by improving their sleep quality and eating habits. Evidence-based interventions regarding the effects of the pandemic should be made for students under the leadership of a psychiatric nurse, and effective strategies must be developed accordingly.
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MakaleAçık Erişim2024/r/ebyu/12732
Investigation of Newly Synthesized Fluorinated Isatin-Hydrazones by In Vitro Antiproliferative Activity, Molecular Docking, ADME Analysis, and e-Pharmacophore Modeling
Başaran, Eyüp; Köprü, Semiha; Akkoç, Senem; Türkmenoğlu, Burçin
In this study, we investigated the in vitro antiproliferative activities and performed computational studies of newly synthesized fluorinated isatin-hydrazones. The chemical structures of the synthesized compounds were confirmed by FT-IR, 1D NMR (1H- and 13C NMR and APT), 2D NMR (HETCOR and HMBC), and elemental analysis. All compounds (1-15) were tested in human lung (A549) and liver (HepG2) cancer cell lines for 72 h. The compounds were screened against a healthy embryonic kidney cell line (HEK-293T) under the same conditions to determine their toxic effects. According to the results obtained, one of the compounds, in particular, compound 8 was effective at inhibiting the growth of cancerous cells, and its effects on both cancer cell lines were similar to IC50 values of 42.43 and 48.43 μM for A549 and HepG2, respectively. Compound 8, which was determined to be the best anticancer agent in vitro, was chosen to interact with the target via molecular docking. This selected ligand (compound 8) interacted with the targets 4HJO, 4ASD, 3POZ, and 7TZ7, and docked into the active sites. The docking score, Glide energy, and Glide emodel values were calculated and determined to be lower than those of the reference compound cisplatin. The pharmacokinetic properties, stability, and drug-likeness parameters of all designed compounds were estimated using SwissADME. Finally, the binding affinities of compound 8 for all four targets were calculated using the MM-GBSA method.
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MakaleAçık Erişim2024/r/ebyu/12730
How does rest interval duration affect performance? An experiment on high-intensity sprint exercises
Akcan, İbrahim Orkun; Ölmez, Cengiz
Introduction: The purpose of this study was to examine the effects of interval and high-intensity sprint training (RST) with different rest intervals on the respiratory function, aerobic and anaerobic power. (2) Materials and Methods: The study involved the voluntary participation of 20 male participants. The participants were divided into two groups: 2IRG (2-minute rest between repeated sprints) and 4IRG (4-minute rest between repeated sprints). The changes in the respiratory function, aerobic, and anaerobic power parameters were examined in the 2IRG and 4IRG athletes who performed two training sessions per week for 4 weeks. (3) Results: The findings indicated that there was no improvement in the respiratory function among the athletes, but there was a significant improvement in aerobic and anaerobic power parameters. However, this improvement was similar in both the 2IRG and 4IRG groups. (4) Conclusions: A 4-week high-intensity interval training program with two RST sessions per week was insufficient to elicit improvements in the respiratory function. However, RST protocols with 2-minute and 4-minute rest intervals provided similar aerobic and anaerobic benefits.
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MakaleAçık Erişim2024/r/ebyu/12728
Paediatric liver biopsies: A single-centre experience in Erzincan Binali Yıldırım University
Kazci, Omer; Kadirhan, Ozlem; Uner, Cigdem; Karavas, Erdal; Ucan, Berna; Aydin, Sonay
Introduction: Liver biopsies are the main method in the diagnosis and treatment of paediatric liver pathologies. Major complication rates of paediatric liver biopsies range from 0% to 6.6% in the literature and minor complication rates range from 0% to 25%. In this study, we aimed to review the complications, indications and results of percutaneous core liver biopsies with paediatric sonography in a tertiary care centre by an interventional radiologist. Methods: We retrospectively evaluated the results, indications and complications of paediatric liver biopsies performed in our tertiary health centre between January 2017 and December 2020. Biopsies were performed with a 16G semi-automatic needle in 17 patients (29.8%) and with an 18G semi-automatic needle in 40 patients (70.2%). Biopsies were performed only with local anaesthesia in patients older than 12 years; in younger patients, it was performed under general anaesthesia. Results: Fifty-eight liver biopsies were obtained from 57 children (34 males, 23 females). The most common indications were elevated liver enzymes (33 patients), cholestasis (14 patients), and adiposity and metabolic problems (6 patents). The most common pathological diagnoses were chronic hepatitis (33 patients) and steatosis (10 patients). Major complication in the form of symptomatic subcapsular haematoma developed after liver biopsy performed with 18G needle in only one patient (1.8%). Conclusions: As previously stated in the literature, percutaneous biopsies performed by interventional radiologists in paediatric patients under the guidance of sonography can be used in diagnosis and treatment; the complication rate is low and it is a safe method.
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MakaleAçık Erişim2024/r/ebyu/12725
Temporal Assessment of Gadolinium Deposition and T1 Signal Intensity Changes in Rat Kidney with Single and Multiple Doses of Injection: An Experimental Study
Alper, Fatih; Karaman, Adem; Yalçın, Ahmet; Atila, Alptug; Dincer, Busra; Sirinoglu, Busra Diyarbakir; Kaya, Serhat; Tavaci, Taha
Background: Gadolinium deposition in biological tissues was first reported in patients with renal failure. We aimed to investigate gadolinium deposition in the rat kidney after exposure to single and multiple doses of gadolinium and evaluate deposition for 1-and 3-month periods. We also aimed to determine any correlation between the amount of deposition and T1-weighted image intensity. Methods: Seventy rats (5 animals per group) were included in the sample, and 9 groups received a single dose (0.3, 0.6, and 1.2 mmol/kg) of gadolinium, and 1 group for each dose was sacrificed at the end of the first day, week, and month. Four groups received weekly doses (0.3 and 0.6 mmol/kg) and were sacrificed at the end of 6 and 12 weeks. Measurement of T1 intensities was carried out with postinjection images before sacrifice, and deposition was determined using inductively coupled plasma mass spectrometry. Results: The number of injections was associated with increased gadolinium deposition (P <.001) in the kid-ney. After the weekly injections, the deposited gadolinium levels did not significantly differ between the low and medium doses at the end of the sixth week (P =.067). There was no agreement between the observers regarding the measurement of T1 signal intensity in both single-dose and multidose experiments (P =.263 and P =.307, respectively). Conclusion: Deposition was dose dependent in the postinjection stage in contrast to the late stage in which deposition was not associated with dose or number of injections until the 12th week. T1 signal intensity measurement is unreliable for assessing deposition in the rat kidney.
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MakaleYalnız künye2024/r/ebyu/12724
A Case of Mediterranean Spotted Fever with Cerebral Infarction
Binay, Umut Devrim; Barkay, Orçun; Karakeçili, Faruk; Gül, Özlem; Tanoğlu, Ceyda; Sümer, Betül
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MakaleAçık Erişim2024/r/ebyu/12720
Antiproliferative effects of 5FU-AgNPs on different breast cancer cells
Danışman-Kalındemirtaş, Ferdane; Kariper, İ. Afşin; Üstündağ, Hilal; Özsoy, Ceylan; Erdem-Kuruca, Serap
Breast cancer is a leading cause of cancer-related deaths in women, and researchers are seeking more effective treatments. Nanotechnology offers a promising approach by creating nanocarriers to reduce the side effects of cancer drugs. This study synthesized AgNPs-5FU nanoparticles by binding the anticancer drug 5-fluorouracil (5FU) to a silver nanocarrier, significantly increasing 5FU's cytotoxicity. Characterization using DLS, SEM, UV-Vis, and FTIR confirmed the nanoparticles' properties, with a size of 18–28 nm and a PDI of 0.598. The release of 5FU from AgNPs-5FU was about 70%, demonstrating sustained and controlled release. Cytotoxicity results showed that AgNPs-5FU had an IC50 value of 23.006 in MCF-7 cells and 10.41 in 4T1 cells, making it 2.14 and 4.64 times more effective than free 5FU, respectively. Low cytotoxicity was observed in HUVEC cells. This study indicates that AgNPs-5FU has significantly higher potential activity against cancer cells compared to free 5FU.
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