Yazarlar
- Suleyman, Halis(167)
- Mammadov, Renad(95)
- Türkeş, Cüneyt(84)
- Aydin, Sonay(83)
- Altuner, Durdu(74)
- Suleyman, Bahadir(68)
- Isik, Arda(67)
- Beydemir, Şükrü(66)
- Kantarci, Mecit(64)
- Kurt, Nezahat(57)
- Demir, Yeliz(54)
- Bulut, Seval(51)
- Arslan, Yusuf Kemal(50)
- Sofu, Hakan(50)
- Coban, Taha Abdulkadir(49)
- Karakeçili, Faruk(47)
- Altay, Ahmet(46)
- Huyut, Mehmet Tahir(46)
- Aydin, Suleyman(45)
- Türkmenoğlu, Burçin(45)
- Kuzucu, Mehmet(40)
- Yazici, Gulce Naz(40)
- Peker, Kemal(39)
- Algul, Oztekin(38)
- Cicek, Betul(37)
- Mertoglu, Cuma(36)
- Camurcu, Yalkin(35)
- Suleyman, Zeynep(34)
- Bozkurt, Aliseydi(32)
- Mendil, Ali Sefa(32)
- Gunay, Murat(31)
- Gözcü, Sefa(31)
- Köksal, Ekrem(31)
- Yuca, Hafize(31)
- Dilek, Esra(30)
- Karakurt, Papatya(30)
- Ozcicek, Fatih(30)
- Süleyman, Halis(30)
- Onk, Didem(29)
- Ucpunar, Hanifi(29)
Yayınlar
- MakaleAçık Erişim2025Investigation of Bromelain, Inulin and Saccharomyces cerevisiae Effects Against Escherichia coli Infection Developed in Fibroblast Wound Model; [Bromelain, İnulin ve Saccharomyces cerevisiae’nin Fibroblast Yara Modelinde Gelişen Escherichia coli Enfeksiyonuna Karşı Etkilerinin Araştırılması]
Başer, Sümeyye; Çelebi, Demet; Çelebi, Özgür; Genç, Sıdıka Tülay; Taghizadehghalehjoughi, Ali
Aim: Wound infections, especially in today’s world where antibiotic resistance is increasing, pose a serious health problem. Escherichia coli complicates treatment by forming a biofilm. In this study, the individual and combined effects of bromelain, inulin, and Saccharomyces cerevisiae on an Escherichia coli infection model created in fibroblast cells were investigated. Methods: The antibacterial activity of bromelain, inulin, and Saccharomyces cerevisiae was evaluated using the disk diffusion method; minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and fractional inhibitory concentration (FIC) analyses were conducted. Biofilm inhibitory activity was evaluated using the crystal violet staining method. In vitro wound model has been created using human fibroblast cells. Bromelain, inulin, and Saccharomyces cerevisiae agents were applied to the cells infected with Escherichia coli. Cell viability was determined by the MTT test and analyzed through measurements of total oxidant status (TOS) and total antioxidant capacity (TAC). To evaluate the inflammatory response, IL-1β and IL-10 levels were quantitatively measured using the ELISA method. Results: According to disk diffusion, MIC/MBC, FIC, and antibiofilm analyses, it has been determined that the triple combination (Bromelain + Inulin + Saccharomyces cerevisiae) provides the highest antibacterial and synergistic effect. In the antibiofilm tests, bromelain showed the strongest inhibitory effect (58.3%), but the effect of the triple combination was more limited. In the MTT analysis, cell viability increased in the treatment groups, reaching 112% especially in the triple combination. While TOS levels increased with infection, they significantly decreased after treatment, whereas TAC levels increased. In ELISA analyses, the IL-1β level significantly increased with infection, but decreased with treatment. IL-10 levels, on the other hand, significantly increased, especially in the triple combination, demonstrating an anti-inflammatory response. Conclusions: The combination of bromelain, inulin, and Saccharomyces cerevisiae has shown antimicrobial, antioxidant, and anti-inflammatory activity against Escherichia coli infection, thereby supporting wound healing.
- MakaleAçık Erişim2025Diagnostic value of super microvascular imaging in differentiating axillary lymph nodes: semi-quantitative and qualitative approach in primary breast cancer and suspicious axillary nodes
Tokur, Oguzhan; Aydin, Sonay; Kilinc, Funda; Degirmenci, Tulin; Oztekin, Pelin Seher
Background: The evaluation of axillary lymph nodes (ALNs) using ultrasound (US) plays a crucial role in the diagnosis and management of breast cancer. This study aims to assess the diagnostic performance of both quantitative and qualitative features of suspicious ALNs using superb microvascular imaging (SMI). To the best of our knowledge, this is the first prospective study in the literature to evaluate the largest number of suspicious ALNs using SMI and to analyze the combined diagnostic value of these findings. Methods: Our prospective study involved 102 patients with suspicious lymph nodes in the axilla. SMI method was employed individually to assess distribution, appearance, and number of vascular structures. After histopathological or follow-up confimation, images were re-evaluated. We also examined the diagnostic value of various combinations of these vascular features. Lastly, two radiologists, each with 22 and 4 years of experience, independently reviewed the images, blinded to clinical and pathological data, to assess interobserver agreement. Results: A total of 108 lymph nodes were evaluated in 102 patients, including 67 benign and 41 malignant cases. The diagnostic performance of various combinations in receiver operating characteristic (ROC) analysis revealed that the highest accuracy (78.70%) and area under the curve (AUC) value in ROC analysis were achieved by distribution-appearance combinations (AUC: 0.776, p < 0.001). Interobserver agreement was significant for both the SMI and PDUS methods. In patients diagnosed with breast cancer, the diagnostic accuracy for suspicious axillary lymph nodes reached 92.76%, with an AUC value of 0.855. Conclusion: In conclusion, our findings suggest that SMI facilitates more accurate identification of suspicious axillary lymph nodes prior to surgical intervention.
- MakaleAçık Erişim2025Relationship Between the Duration of Intravenous Ketamine Anesthesia and Postoperative Oxidative Stress and Inflammatory Response in Rats
Ince, Ramazan; Ozgodek, Habip Burak; Kahramanlar, Agah Abdullah; Yucel, Nurinisa; Sarıgül, Cengiz; Suleyman, Halis
Surgical trauma triggers oxidative and inflammatory responses that contribute to postoperative complications. Although the antioxidant and anti-inflammatory effects of ketamine have been reported, the impact of anesthesia duration on these mechanisms remains unclear. Forty-two male Wistar rats were randomized into healthy control (HG), ketamine only (KET; 60 mg/kg, i.p.), or laparotomy plus ketamine with 0–4 additional ketamine doses at 20 min intervals (KET + L, KET + L1–L4). At 24 h, levels of MDA, tGSH, SOD, CAT, IL-1β, IL-6, TNF-α, adrenaline and noradrenaline were measured in tail-vein blood. One-way ANOVA with Tukey’s post hoc test was used. Laparotomy under single-dose ketamine increased MDA and pro-inflammatory cytokines and decreased tGSH, SOD, CAT, ADR, and NDR versus HG and KET (all p < 0.001). After laparotomy, repeated ketamine dosing produced graded decreases in MDA and cytokines and increases in tGSH, SOD, CAT, ADR, and NDR toward control levels; effects were most pronounced in KET + L4 (all p < 0.001). Ketamine alone did not differ significantly from HG. In rats, ketamine modulates postoperative biological stress in a duration-dependent manner; prolonging anesthesia reduces oxidative–inflammatory load and restores catecholaminergic tone. These findings strongly support revisiting dose–duration protocols and underscore the need for mechanistic and clinical studies.
- MakaleAçık Erişim2025ML-based tooth shade assessment to prevent metamerism in different clinic lights
Karcioglu, Abdullah Ammar; Efitli, Esra; Simsek, Emrah; Ozdogan, Alper; Karatas, Furkan; Senocak, Tuba
The aesthetic understanding has found its place in dental clinics and prosthetic dental treatment. Determining the appropriate prosthetic tooth color between the clinician, patient and technician is a difficult process due to metamerism. Metamerism, known as the different perception of the color of an object under different light sources, is caused by the lighting differences between the laboratory and the dental clinic. The traditional trial-error color determination method, coupled with the high cost of instrumental color value determination, has prompted the need for alternative technologies. The integration of AI technologies into dental practices aims to minimize errors in tooth shade assessment, reduce equipment usage, eliminate the impact of clinic lighting on color detection, and decrease costs for patients, dentists, and laboratories. In this study, a machine learning (ML) based approach that can correctly detect tooth shade even under different clinical lights has been developed. A dataset consisting of 580 dental images taken under four different clinical lights and with five repetitions was created using the Vita color shade guide. Experimental studies were performed using the HSV color space, 6 different ML algorithms and color histograms. As a result, 97.93% accuracy rate was achieved by using cross-validation (cv = 5) in the classification of 29 color values independent of clinical lights. It has been shown that the tooth colors can be determined with high accuracy using ML algorithms and metamerism can be prevented.
- MakaleYalnız künye2025Turkish Adaptation of Social Media Questionnaire for Nursing Training
Erden, Yasemin; Dogan, Sevgi; Aydın Kahraman, Handan; Çiftçi, Bahar
Background and Purpose: The use of social media in education has many effects such as rapid dissemination of information, crowdsourced information sharing, learning, and social interaction. In the study, it was aimed to determine the validity and reliability of the Social Media Questionnaire for Nursing Training (SMQNT), which can be used to determine the effect of social media in nursing education. Methods: The data of this methodological study were obtained from 262 nursing students. Confirmatory factor analysis (CFA) was used to determine the scale’s factor structure, while Cronbach’s alpha, test–retest method, and split-half reliability method were used for reliability. Results: In line with the results of the CFA, it was determined that the scale had 13 items and a three-factor structure as “attitudes,” “use,” and “contrast information.” Similar to the original scale, a three-factor establishment was approved. SMQNT Cronbach’s alpha was found to be.87, while Cronbach’s alpha was found to be.658 for the “F1” factor,.760 for the “F2” factor, and.688 for the “F3” factor. Conclusions: As a result of this study, the Turkish form of SMQNT was a valid and reliable measurement instrument.
- MakaleAçık Erişim2025In Vitro Evaluation of Antidiabetic, Antioxidant, and Anticholinergic Properties of Different Parts of Bilberry (Vaccinium myrtillus L.) From the Eastern Black Sea Region, With LC/MS–MS Analysis of Their Chemical Composition in Food Applications
Yuca, Hafize; Ayar, Beyzanur; Aydın, Bilge; Çoban, Furkan; Karakaya, Songül
Diabetes, the most prevalent metabolic disorder, is closely linked to Alzheimer's disease, with DM patients showing a two-fold increased risk of developing AD. Bilberry (Vaccinium myrtillus L.) is a prominent wild berry, widely used in juice and food production. This study aimed to evaluate and compare the phytochemical composition and biological activities of different parts of wild V. myrtillus plants, including branches, leaves, and fruits. Using LC–MS/MS, the study quantifies total phenolics, flavonoids, and tannins in each part. Biological activities are assessed through antioxidant activity (ABTS and DPPH assays), anticholinesterase (neuroprotective potential), and antidiabetic effects. The experiments revealed that the Leaf of Yer Ligarba (YLL) extract had the highest values in terms of total phenolic content, total flavonoid content, and total tannin content. Among the extracts, the highest radical scavenging effect was observed with YLL at 86.67% against DPPH. The leaf extracts, Leaf of Dal Ligarba (DLL) and YLL, demonstrated the highest scavenging effects, with inhibition rates of 99.37% and 99.37%, respectively. At one of the common concentrations, 100 μg/mL, acarbose showed 13.12% inhibition, while the Branch of Dal Ligarba (DLB) extract exhibited 98.67% α-glucosidase enzyme inhibition. The highest acetylcholinesterase and butyrylcholinesterase enzyme inhibition activities were observed in the Branch of Yer Ligarba (YLB; 27.08%) and the Fruit of Dal Ligarba (DLF; 16%), respectively. Chlorogenic acid was the most abundant phenolic compound, particularly in the DLL 58,569.80 ng/mL. This study highlighted the significant phytochemical content and biological activities of wild bilberry extracts, demonstrating their potential for managing diabetes and neurodegenerative diseases, particularly Alzheimer's.
- MakaleAçık Erişim2025Analyzing Quinoa Seeds: LC–MS/MS and GC–MS/MS Approaches for Identifying a Nutrient-Rich Functional Food in Alzheimer's and Diabetes Care
Çoban, Furkan; Yuca, Hafize; Aydın, Bilge; Tosun, Murat; Yazıcı, Abdullah; Karakaya, Songül; Tan, Mustafa; Özer, Hakan; Atila, Alptuğ
Quinoa is a versatile, nutrient-dense, gluten-free pseudocereal, increasingly recognized for its potential health benefits, particularly in managing diabetes mellitus and Alzheimer's disease—two conditions with growing links. Recent research on 14 quinoa genotypes explored fatty oil composition and bioactive potential of quinoa extracts. Linoleic acid was predominant fatty acid (46.60%–56.33%), whereas alanine was the most concentrated amino acid across all active extracts (192.4887–1578.0355 nmol/mL). French Vanilla hexane extract exhibited the strongest α-glucosidase inhibition (75.71%), with Santa Ana hexane extract showing the highest α-amylase inhibition (28.58%). Additionally, Titicaca hexane extract displayed notable acetylcholinesterase inhibition (22.22%), and Moqu Arochilla methanol extract had the most potent butyrylcholinesterase inhibition (47.20%). Antioxidant assays revealed that Salcedo and French Vanilla extracts had exceptional 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, with positive correlations found between total phenolic content and antioxidant activity (DPPH, β-carotene, FRAP). These findings emphasize quinoa's potential as a functional food, offering significant benefits for disease management.
- MakaleAçık Erişim2025Nanoemulsion Hydrogel Delivery System of Hypericum perforatum L.: In Silico Design, In Vitro Antimicrobial–Toxicological Profiling, and In Vivo Wound-Healing Evaluation
Kurt, Ahmet Arif; Ibrahim, Bashar; Çınar, Harun; Atsü, Ayşe Nilhan; Bursalıoğlu, Ertuğrul Osman; Bayır, İsmail; Özmen, Özlem; Aslan, İsmail
Hypericum perforatum L. (H.P.), a plant renowned for its wound-healing properties, was investigated for antioxidant/antimicrobial efficacy, toxicological safety, and in vivo wound-healing effects in this research to develop and characterize novel nanoemulsion hydrogel (NG) formulations. NG were prepared via emulsion diffusion–solvent evaporation and polymer hydration using Cremophor RH40 and Ultrez 21/30. A D-optimal design optimized oil/surfactant ratios, considering particle size, PDI, and drug loading. Antioxidant activity was tested via DPPH, ABTS+, and FRAP. Toxicological assessment followed HET-CAM (ICH-endorsed) and ICCVAM guidelines. The optimized NG-2 (NE-HPM-10 + U30 0.5%) demonstrated stable and pseudoplastic flow, with a particle size of 174.8 nm, PDI of 0.274, zeta potential of −23.3 mV, and 99.83% drug loading. Release followed the Korsmeyer–Peppas model. H.P. macerates/NEs showed potent antioxidant activity (DPPH IC50: 28.4 µg/mL; FRAP: 1.8 mmol, Fe2+/g: 0.3703 ± 0.041 mM TE/g). Antimicrobial effects against methicillin-resistant S. aureus (MIC: 12.5 µg/mL) and E. coli (MIC: 25 µg/mL) were significant. Stability studies showed no degradation. HET-CAM tests confirmed biocompatibility. Histopathology revealed accelerated re-epithelialization/collagen synthesis, with upregulated TGF-β1. The NG-2 formulation demonstrated robust antioxidant, antimicrobial, and wound-healing efficacy. Enhanced antibacterial activity and biocompatibility highlight its therapeutic potential. Clinical/pathological evaluations validated tissue regeneration without adverse effects, positioning H.P.-based nanoemulsions as promising for advanced wound care.
- MakaleAçık Erişim2025The effect of body mass index on maternal and neonatal health in term pregnancies: a cross-sectional study in Turkey
Yilmaz, Ayşe Daştan; Çalik, Kıymet Yeşilçiçek; Budak, Mehtap
Backgroud and objective: Maternal obesity and gestational weight gain (GWG) are important determinants of maternal and neonatal health outcomes. This study aimed to investigate the effects of prepregnancy body mass index (PPBMI) and GWG on maternal and neonatal outcomes in Türkiye. Methods: This prospective cross-sectional study was conducted in a public hospital in Turkey between December 2021 and December 2022. A total of 1137 women between the ages of 19 and 45 who had singleton and live births and had complete medical records were included in the study using simple random sampling. Data were collected using medical records and a structured interview form. PPBMI and GWG were calculated from self-reported weight and height data. Kruskal-Wallis, Mann-Whitney U, and binary logistic regression tests were used to analyze the data. Results: Among the participants, 4.7% were underweight, 61% had a normal weight, 27.6% were overweight, and 9.9% were obese on the basis of PPBMI. According to the Institute of Medicine (IOM) GWG guidelines, 17.2% had insufficient GWG, 55.1% had adequate GWG, and 27.8% had excessive GWG. Overweight women had significantly increased odds of gestational diabetes mellitus (OR = 0.479, p = 0.024), genital tract infections (OR = 2.15, p = 0.000), urinary tract infections (OR = 2.42; p = 0.011), elective cesarean delivery (OR = 8.62, p = 0.035), macrosomia (OR = 9.15, p = 0.031), and low APGAR (Appearance, Pulse, Grimace, Activity, Respiration) scores (< 7 at 5 min) (OR = 0.20, p = 0.000). Obese women also showed higher odds of elective cesarean (OR = 9.56; p = 0.030) and macrosomia (OR = 8.27, p = 0.044). Underweight women had higher odds of neonatal hospital stay > 5 days. Insufficient GWG was associated with increased risks of low birth weight (OR = 0.46, p = 0.013), third-trimester bleeding (OR = 0.45, p = 0.016), and neonatal hospitalization > 5 days (OR = 0.44, p = 0.003), while macrosomia risk was lower (OR = 2.06, p = 0.001). Conclusion: PPBMI and GWG are significant predictors of maternal and neonatal health outcomes. Elevated PPBMI and inappropriate GWG are associated with increased risks of gestational diabetes, macrosomia, cesarean delivery, low APGAR scores, low birth weight, and prolonged neonatal hospitalization. These findings highlight the critical role of routine assessment and management of maternal weight status and weight gain during prenatal care in reducing preventable complications for both mothers and their newborns.
- MakaleYalnız künye2025A new series of naphthyl-thiosemicarbazone and thio/carbohydrazone derivatives: Synthesis, spectroscopic elucidation, dual enzyme inhibition targeting carbonic anhydrase/ acetylcholinesterase and molecular docking studies
Yakan, Hasan; Muğlu, Halit; Erdoğan, Musa; Türkeş, Cüneyt; Demir, Yeliz
New naphthyl-thiosemicarbazone derivatives (1–9) were obtained from 1-naphthaldehyde and numerous thiosemicarbazides. New naphthyl-thio/carbohydrazones (10–12) were prepared from 1-naphthaldehyde and various thio/carbohydrazides. FT-IR, 1H NMR, 13C NMR, and elemental analysis were used to elucidate the structures of the newly obtained compounds. The inhibitory effects of these compounds against human carbonic anhydrase isoforms I and II (hCA I and hCA II) and acetylcholinesterase (AChE) were systematically evaluated. Several compounds exhibited potent inhibition, particularly derivatives bearing halogenated and electron-donating aromatic substituents. Among them, compound 11 demonstrated the strongest inhibition for all three enzymes, with KI values of 52.42 nM (hCA I), 59.23 nM (hCA II), and 40.16 nM (AChE), surpassing the reference standards acetazolamide and tacrine. Structure–activity relationship (SAR) analysis highlighted the critical influence of substituent type and position on enzymatic activity. In addition, molecular docking simulations were conducted to elucidate the binding interactions of the most potent compound with hCA I, hCA II, and AChE enzymes. The docking results supported the in vitro findings, revealing favorable binding energies and key interactions such as π–π stacking and hydrogen bonding, especially for compound 11.
- MakaleAçık Erişim2025Protective effect of adenosine triphosphate against hydroxychloroquine ototoxicity in rats
Erhan, Ertugrul; Suleyman, Zeynep; Altuner, Durdu; Demir, Ozlem; Gulaboglu, Mine; Suleyman, Halis
One of the side effects of hydroxychloroquine is ototoxicity. This study examined the effect of adenosine triphosphate (ATP) on ototoxicity in rats treated with hydroxychloroquine (HCQ). In this study, 24 rats (male, albino Wistar) were used. The animals were divided into four groups: healthy control group (HG), ATP alone group (ATPG), HCQ alone group (HCQG), and ATP + HCQ (AHQG) group. The ATPG and AHQG groups were injected with ATP intraperitoneally (IP) at a dose of 4 mg/kg/day. The HG and HCQG groups were treated with the same volume of saline as the solvent by IP route. The AHQG group was administered HCQ orally at a dose of 120 mg/kg/day 1 h after the ATP and saline administration. This procedure was repeated daily for 10 days. The rats were euthanized (50 mg/kg thiopental sodium, IP) and after, vestibulocochlear nerve tissues were removed and the tissue oxidant and antioxidant parameters were studied. The tissues were also subjected histopathological examination. ATP significantly (p < 0.001) suppressed the increase in malondialdehyde associated with HCQ in vestibulocochlear nerve tissues as well as the decrease in total glutathione, superoxide dismutase, and catalase levels. ATP also reduced the severity of oedema, vascular congestion, dilatation, and collagen deposition in degenerated myelinated nerve fibers associated with HCQ. ATP is effective against oxidative damage associated with HCQ in vestibulocochlear nerve tissue.
- MakaleYalnız künye2025Protective effect of deinoxanthin in sorafenib-induced nephrotoxicity in rats with the hepatocellular carcinoma model
Karasu, Nilgun; Kuzucu, Mehmet; Mat, Ozge Cengiz; Gul, Mustafa; Yay, Arzu; Dundar, Munis
Sorafenib is a synthetic compound and an orally administered multichines inhibitor that targets growth signaling and angiogenesis. It is widely recognized as the standard of care for advanced hepatocellular carcinoma (HCC) but has toxic side effects. Deinoxanthin, purified from the radioresistant bacterium Deinococcus radiodurans, has strong antioxidant characteristics. In this study, the protective effect of deinoxanthin against sorafenib-induced nephrotoxicity was investigated in a rat model of hepatocellular carcinoma. In this regard, the expressions of DDAH1, KIM1, and INOS genes were examined, histopathological and immunohistochemical analyses were performed, and various parameters such as SOD, MDA, GST, CAT, TAS, and TOS were tested biochemically. BUN and creatinine levels were measured in renal tissues. RT-qPCR, Western blot, and ELISA methods were used for all these analyses. As a result, the analyses show that deinoxanthin, which has a high antioxidant capacity, reduces kidney injury and can be used as a protective agent. The primary objective of this study is to evaluate the potential of deinoxanthin as a protective agent against the nephrotoxic side effects of sorafenib in HCC. Our study identified the potential synergistic effects of sorafenib and deinoxanthin on nephrotoxicity in rats with hepatocellular carcinoma.
- MakaleAçık Erişim2025Evaluation of Treatment Response in Chronic Hepatitis C Patients Receiving Sofosbuvir/Velpatasvir/Voxilaprevir: A Multicenter Real-World Experience from Türkiye
Binay, Umut Devrim; Karakeçili, Faruk; Aygen, Bilgehan; Kılınç Toker, Ayşin; Çelik, İlhami; Demirtürk, Neşe; Şimşek Bozok, Tuğçe; Dursun, Leyla; Akgül, Fethiye; Çınar, Güle
The combination of sofosbuvir/velpatasvir/voxilaprevir (SOF/VEL/VOX) is recommended as a salvage therapy for treatment-experienced chronic hepatitis C (CHC) patients. However, it is used in our country for treatment-naïve and treatment-experienced patients. This study aims to present real-world data from Türkiye on CHC patients treated with SOF/VEL/VOX. The present study was conducted by the Viral Hepatitis Study Group of the Turkish Society of Clinical Microbiology and Infectious Diseases (KLİMİK). It was a multicenter, retrospective, observational study. The data were collected from patients receiving SOF/VEL/VOX therapy at 12 medical centers in Türkiye between 1 June 2022 and 31 December 2024. The patients had received the treatment for 8 to 12 weeks. Of the 139 patients enrolled, 63.3% (n = 88) were male, with a mean age of 54.4 years. Most patients were non-cirrhotic (94.2%, n = 131) and treatment-naïve (92%, n = 128); 49.6% (n = 69) were infected with genotype 1b. Early virologic response (EVR) could be assessed in 126 patients, with an EVR rate of 82.5% (n = 104). End-of-treatment data were available for 113 patients, all achieving an end-of-treatment response. Among the 80 patients for whom week-12 post-treatment data were available, 97.5% sustained virologic response at week 12 (SVR12). Significant improvements were observed in AST, ALT, and platelet levels, along with reductions in APRI and FIB-4 scores (p = 0.001).” No serious adverse events leading to treatment discontinuation were reported. Mild adverse events included pruritus (2.1%, n = 3), fatigue (2.1%, n = 3), and nausea (1.4%, n = 2). The SOF/VEL/VOX combination is a highly effective and well-tolerated treatment option in treatment-naïve CHC patients, achieving an SVR12 rate of 97.5%.
- MakaleYalnız künye2025Analysis of possible effects of recombinant antimicrobial peptide TP4 on colon cancer line HT-29
Kılınç, Seda; Karaoğlan, Mert; Kuzucu, Mehmet
Tilapia piscidin 4 (TP4) is a 25-amino-acid cationic antimicrobial peptide derived from Nile tilapia (Oreochromis niloticus). TP4 has demonstrated strong antimicrobial activity against a broad range of pathogens and exhibits additional biological functions, including wound healing and anticancer activity. In a previous study, recombinant TP4 peptide was produced in the Pichia pastoris KM71H expression system and purified by Ni–NTA affinity chromatography. The aim of this study was to evaluate and compare the cytotoxicity of recombinant TP4 against HT-29 colon cancer and HGF-1 healthy gingival fibroblast cells.The antimicrobial activity of TP4 was first assessed using minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays, confirming its efficacy. Subsequently, the cytotoxic effects of TP4 on HT-29 and HGF-1 cells were further analyzed using the MTT, lactate dehydrogenase (LDH), total antioxidant/oxidant status (TAS/TOS), and malondialdehyde (MDA) assays. TP4 disrupted the antioxidant balance in both cell types, with a significant increase in the oxidant levels and a marked decrease in the antioxidant levels in HT-29 cells. The IC50 values of TP4 were determined to be 15 μg/mL for HT-29 cells and 25 μg/mL for HGF-1 cells. Apoptosis assays, including Annexin V & Dead Cell, RT-qPCR, and ELISA, revealed that TP4 induced apoptosis predominantly in HT-29 cells, as evidenced by the upregulation of BAX and CASP3 and the downregulation of the BCL-2. In conclusion, TP4 exhibited selective and potent apoptotic effects on HT-29 colon cancer cells, suggesting its potential as a novel therapeutic agent for colon cancer treatment.
- MakaleAçık Erişim2025Molecular Characterization of Echinococcus granulosus Isolated from Clinical Samples of Patients Diagnosed with Hydatid Cysts in Eastern Türkiye
Yılmaz, Ahmet; Aydın, Hakan; Akkaş, Önder; Aydın, Yener; Eren, Suat; Uslu, Hakan
Background: We aimed to investigate the genetic diversity and population structure of Echinococcus granulosus using various cyst materials from the parasite obtained from humans in eastern Türkiye. Methods: We used cyst samples from 61 patients diagnosed with hydatid cysts by the Department of Thoracic Surgery and Department of Radiology at Atatürk University Faculty of Medicine between 2019 and 2023. Microscopic examination of protoscolex in cyst samples was performed, and, if available, the patients' ELISA IgG results were obtained from the hospital automation system. Two primer pairs revealed DNA regions specific to E. granulosus (12S rRNA and mt-COX1). Sequence analysis was performed on samples found positive for mt-COX1 PCR, and a phylogenetic tree was constructed from the resulting data. Results: The rate of scolex in microscopic examination was 31/61 (50.8%), and the ELISA IgG positivity rate was 36/51 (70.6%). As a result of PCR analysis of 12S rRNA and mt-COX1 gene regions of 61 cyst samples, positivity was determined in 48 (78.7%) samples. As a result of PCR product sequence analysis, it was found that all samples were of the E. granulosus sensu stricto (G1, G3) genotype. Lung cyst alone was detected in 25 (40.9%) cases, while only liver cyst was detected in 15 (24.6%) cases. In 13 (21.3) cases, cysts were present in both the lungs and the liver. Conclusion: The genotype of E. granulosus isolates obtained from cyst material from patients diagnosed with hydatid cysts in Erzurum and surrounding provinces was a domestic sheep strain (G1, G3).
- MakaleAçık Erişim2025Investigation into the Effects of Tramadol, Citalopram, Tianeptine, and Their Combinations on Rat Brain Tissue
Ates, Irem; Isik, Bahar; Gozen, Fusun; Yazici, Gulce Naz; Gulaboglu, Mine; Mammadov, Renad; Huseynova, Gulbeniz; Altuner, Durdu; Suleyman, Halis
Background: Tramadol binds to opioid receptors and inhibits norepinephrine and serotonin reuptake, causing serotonin syndrome. Tianeptine stimulates serotonin reuptake and reduces serotonin levels. The aim of this study was to investigate whether tianeptine is effective against serotonin syndrome that may occur with serotoninergic drugs such as tramadol and citalopram. Methods: Rats were divided into eight groups (n = 6) that received tramadol (50 mg/kg), citalopram (10 mg/kg), or tianeptine (5 mg/kg) alone or a combination of tramadol + citalopram, tramadol + tianeptine, citalopram + tianeptine or tramadol + citalopram + tianeptine at the same doses administered to the stomach by oral gavage for 3 weeks. The healthy control group was given saline. Malondialdehyde, total glutathione, superoxide dismutase, and catalase levels were measured in removed brain tissues. The tissues were also examined histopathologically. Results: In the tramadol, tramadol + citalopram, and tramadol + citalopram + tianeptine groups, malondialdehyde levels were found to be higher compared to the control group, while glutathione, superoxide dismutase, and catalase levels were found to be lower. In other groups, values close to the control group were measured. Morphological degeneration was observed in neurons in the tramadol + citalopram group. The swelling of astrocytes and pericellular edema in oligodendrocytes were also observed. A significant population increase was noted in microglial cells. Blood vessels belonging to the tissue were observed to be severely dilated and congested. Histopathological damage was partially resolved in the group given tramadol + citalopram + tianeptine. Conclusions: The tramadol + citalopram combination caused severe oxidative stress in brain tissue. Tramadol alone caused mild damage in brain tissue, whereas tianeptine prevented the brain damage caused by tramadol.
- MakaleAçık Erişim2025Phenolic Content, Antioxidant Capacity, and Therapeutic Potential of Mango (Mangifera indica L.) Leaves
Işık, Mesut; Dikici, Emrah; Altın, Sevgi; Alp, Cemalettin; Kırboğa, Kevser Kübra; Köksal, Ekrem; Beydemir, Şükrü
The phenolic composition, antioxidant capacity, and enzyme inhibition activities of Mangifera indica L. leaf (MLs) ethanol extract were comprehensively evaluated to explore its therapeutic and industrial applications. Quantitative profiling of 21 phenolic compounds was performed using the LC–MS/MS method, with vanillic acid (1242.47 μg/L), gallic acid (283.58 μg/L), and quercetin (102.40 μg/L) identified as the most abundant constituents. Antioxidant activities were assessed through DPPH, ABTS, FRAP, and CUPRAC assays, revealing moderate radical scavenging (DPPH: 26.87% ± 2.25%, ABTS: 14.65% ± 1.83%) and metal reduction capacities (FRAP: 0.118 ± 0.07, CUPRAC: 0.172 ± 0.03). In addition, MLs extract demonstrated dose-dependent inhibitory effects on acetylcholinesterase (AChE, IC50: 18.73 μg/mL), butyrylcholinesterase (BChE, IC50: 8.56 μg/mL), and α-glucosidase (IC50: 10.83 μg/mL), highlighting its potential in the management of neurodegenerative diseases such as Alzheimer's and metabolic disorders including diabetes. The findings emphasize the bioactive potential of M. indica leaves, positioning them as a promising resource for sustainable valorization. By showcasing the applicability of this agricultural by-product, the study provides a foundation for innovations in the food, nutraceutical, and pharmaceutical sectors. Nevertheless, further in vivo and clinical investigations are essential to fully validate their safety and therapeutic efficacy.
- MakaleAçık Erişim2025Enhanced therapeutic efficacy of platinum-doxorubicin nanoparticles on colon and breast cancer cell lines
Kalındemirtaş, Ferdane Danışman; Cilasun, Gökçe Erdemir; Kariper, Afşin
In this study, platinum nanoparticles (PtNPs) were synthesized and their potential to improve the efficacy of doxorubicin (DOX) in cancer treatment was investigated. H2PtCl6, LiAlH4, and trisodium citrate were used during the synthesis of PtNPs. They were characterized using dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR), and scanning transmission electron microscopy (STEM). The diameter of the PtNPs was measured to be 21.72 nm without DOX loading and approximately 212 nm after DOX loading (DOX-PtNPs). FTIR confirmed the binding of DOX to PtNPs. In addition, MTT assays showed that DOX-PtNPs have a stronger effect on MCF-7 and HCT116 cancer cells than free DOX, even at low doses. The IC50 value for MCF-7 cells treated with DOX was determined to be 4.81 µg/ml, while it was significantly lower for the DOX-PtNP group at 0.64 µg/ml. A similar trend was observed in HCT116 cells, where the IC50 value for DOX was 5.03 µg/ml, while for DOX-PtNPs it was 0.62 µg/ml. In summary, the activity of DOX in these cells was increased approximately eightfold by PtNPs. Moreover, DOX-PtNPs showed no significant cytotoxic effects on normal HUVEC cells at low doses. Moreover, DOX-PtNPs enhanced apoptotic activity in HCT116 cells without inducing drug resistance as demonstrated by Rho123 staining and annexin/PI analyses. The significance of this study lies in the pioneering use of DOX-PtNPs in colon cancer, the synthesis of smaller PtNPs, the eightfold increase in the efficacy of DOX, and the demonstration that DOX-PtNPs do not significantly increase drug resistance.
- MakaleYalnız künye2025A Mixture Modeling of the Behavioral Activation System and Problematic Internet Use in Adolescents: The Role of Metacognition, Online Dissociation, and Insomnia
Çelik, Oğuzhan; Yalçın, Ragıp Ümit; Eşkisu, Mustafa
Due to the rapidly developing technological advancements, the overuse of the Internet has led to the emergence of problematic Internet use, which has become a part of our daily lives. This study aims to investigate the relationships between problematic Internet use, Behavioral Activation System (BAS), metacognition, online dissociative experiences, and insomnia. Participants were 341 Turkish adolescents aged between 15 and 19 years (Mean = 15.49, SD = 1.03), of whom 63% were female. Participants responded to the Behavioral Inhibition/Behavioral Activation System Scale, Metacognition Questionnaire, Van Online Dissociative Experiences Schedule, Insomnia Severity Index, and Chen Internet Addiction Scale. The mixture structural equation modeling approach was used to analyze the data. We found a positive relationship between BAS and metacognition, as well as insomnia, and a negative relationship with online dissociation. Furthermore, BAS was indirectly related to problematic Internet use via metacognition, online dissociation, and insomnia. The mixture analysis grouped participants into two latent classes. The first class (76%) had low levels of metacognition, online dissociation, insomnia, and problematic Internet use and high levels of behavioral activation while the second class (24%) had low levels of behavioral activation and high levels of metacognition, online dissociation, insomnia, and problematic Internet use. These findings indicate that individuals with high impulsivity, a tendency toward fun-seeking, and reward sensitivity are predisposed to excessively use the Internet and that metacognition, online dissociative experiences, and insomnia play a determining role in this behavior.
- MakaleAçık Erişim2025Intestinal endometriosis: A rare cause of acute care surgery; [Bağırsak endometriozisi: Acil cerrahinin nadir bir nedeni]
Kutluk, Fadime; Ergün, Sefa; Uludag, Server Sezgin
BACKGROUND: Intestinal endometriosis is a rare condition that can cause gastrointestinal symptoms such as abdominal pain, constipation, and diarrhea. It occurs in approximately 5%–15% of women with endometriosis. Although it rarely leads to obstruction or perforation, there is no clear consensus on its optimal management. Hormonal therapy is considered the first-line treatment for endometriosis; however, the best approach for intestinal involvement remains controversial. This study aims to contribute to the literature by evaluating patients with intestinal endometriosis who underwent surgery for acute abdomen. METHODS: Sixteen patients who underwent emergency surgery for acute abdomen at the Emergency General Surgery Clinic of İstanbul University-Cerrahpaşa, Cerrahpaşa Medical Faculty of Medicine, between February 2016 and April 2023 were identified. Four patients voluntarily withdrew from the study, and 12 were included in the analysis. Demographic data, laboratory findings, surgical records, length of hospital stay, pathology reports, postoperative complications within the first 30 days, and their management were reviewed. Patients’ current health status was assessed through telephone interviews. RESULTS: The mean age of the patients was 39.3±9.2 years. Nine patients underwent surgery with a preoperative diagnosis of intestinal obstruction, while three were operated on for suspected acute appendicitis. Among the patients with intestinal obstruction, three underwent laparoscopic ileocecal resection, and three had segmental small bowel resection. One patient underwent anterior resection with appendectomy, and another underwent both anterior resection and ileocecal resection with ileocolostomy. One patient underwent a left hemicolectomy. The mean hospital stay was 7.9±5.9 days. Postoperative complications occurred in three patients (25%). The mean age of patients who experienced complications was significantly higher than that of those who did not (p<0.007). Histopathological examination revealed benign full-thickness endometriosis in all cases. The mean follow-up period was 50.6 months, with no recurrences observed. CONCLUSION: Acute abdomen due to intestinal endometriosis-related obstruction is extremely rare. In reproductive-aged women presenting with acute abdomen, intestinal endometriosis should be considered in the differential diagnosis. In cases of clinical suspicion, intraoperative consultation with a gynecologist is recommended, and a multidisciplinary approach should be adopted to optimize treatment planning.
