Yazarlar

  • ArticleYalnız künye2017
    Protective role of humic acids against picloram-induced genomic instability and DNA methylation in Phaseolus vulgaris

    Taspinar, Mahmut Sinan; Aydin, Murat; Sigmaz, Burcu; Yildirim, Nalan; Agar, Guleray

    Picloram (4-amino-3,5,6-trichloropicolinic acid) is a liquid auxinic herbicide used to control broad-leaved weeds. Picloram is representing a possible hazard to ecosystems and human health. Therefore, in this study, DNA methylation changes and DNA damage levels in Phaseolus vulgaris exposed to picloram, as well as whether humic acid (HA) has preventive effects on these changes were investigated. Random amplified polymorphic DNA (RAPD) techniques were used for identification of DNA damage and coupled restriction enzyme digestion-random amplification (CRED-RA) techniques were used to detect the changed pattern of DNA methylation. According to the obtained results, picloram (5, 10, 20, and 40 mg/l) caused DNA damage profile changes (RAPDs) increasing, DNA hypomethylation and genomic template stability (GTS) decreasing. On the other hand, different concentrations of applied HA (2, 4, 6, 8, and 10%) reduced hazardous effects of picloram. The results of the experiment have explicitly indicated that HAs could be an alternative for reducing genetic damage in plants. In addition to the alleviate effects of humic acid on genetic damage, its epigenetic effect is hypomethylation.

    • SDG 2 · Açlığa Son
    • SDG 15 · Karasal Yaşam
    RDL Kalıcı Kaydıİncele
  • ArticleAçık Erişim2017
    Genetic diversity of local endemic Teucrium leucophyllum montbret & aucher ex Bentham. (Lamiaceae) in Turkey

    Sözen, Emel; Hilooğlu, Muhip; Kandemir, Ali

    Background and Objective: Teucrium leucophyllum is endemic plant species having considerable narrow distribution in Erzincan (Turkey) Region. The aim of this study was to determine genetic diversity levels of T. leucophyllum in order to obtain data for its conservation. Method: Genomic DNA was isolated from 81 plants using CTAB method. Genetic diversity in four natural populations of T. leucophyllum was investigated by ISSR markers. A binary matrix was produced by scoring each amplified fragment as present (1) or absent (0) from each individual. The matrix was used to produce an input file and analyzed using the software programs; POPGENE 1.32 and GenAlex. Results: Twenty primers amplified a total of 434 bright and discernible bands of 220-3000 bp, of which 431 bands (PPB: 99.31%) were polymorphic in 4 populations. The genetic diversity was high at the species level; the observed number of alleles (Na) was 1.993, the effective number of alleles (Ne) was 1.404, the Nei’s genetic diversity (H) was 0.263, and the Shannon’s information index (I) was 0.418. A high level of gene flow (Nm: 2.208) and low-moderate genetic differentiation (Gst: 0.184) were also observed. Analysis of molecular variance showed that 11% of the total genetic diversity resided among populations, while 89% within the populations. Conclusion: Our results indicated that the conservation efforts should aim to preserve the extant populations of this endangered species in its natural habitat. Seeds could be collected from Kayacik and Bagistas populations for ex situ conservation practices.

    • SDG 15 · Karasal Yaşam
    RDL Kalıcı Kaydıİncele
  • ArticleAçık Erişim2017
    Mapping land use with using Rotation Forest algorithm from UAV images

    Akar, Özlem

    The aim of this study is to test the performance of the Rotation Forest (RTF) algorithm in areas that have similar characteristics by using Unmanned Aerial Vehicle (UAV) images for the production of most up-to-date and accurate land use maps. The performance of the RTF algorithm was compared to other ensemble methods such as Random Forest (RF) and Gentle AdaBoost (GAB). The accuracy assessments showed that the RTF with 84.90% and 93.33% accuracies provided better performance than RF (7% and 4%) and GAB (15% and 11%) in urban and rural areas, respectively. Subsequently, in order to increase the classification accuracy, a majority filter was applied to post-classification images and the overall classification accuracy of the RFT was increased approximately up to 3%. Also, the results of classification were also analysed using the McNemar test. Consequently, this study shows the success of the RTF algorithm in the classification of UAV images for land use mapping.

    • SDG 15 · Karasal Yaşam
    RDL Kalıcı Kaydıİncele
  • ArticleAçık Erişim2015
    Uptake of selected heavy metals and their effects on some physiologic parameters and mineral nutrition in Phragmites Australis in Karasu river-Turkey

    Osma, E.; Ilhan, V.; Yalcin, I.E.

    Water contamination by heavy metals is a serious global problem due to increased environmental and health-related issues. In this study, we have comparatively investigated the heavy metal concentrations and mineral nutrient levels in Karasu river sediments and in plants samples of Phragmites australis (Cav.) in order to assess the biomonitoring capacity. Plant and sediment samples were collected from four different localities along Karasu River, and those samples were analyzed in terms of heavy metals such as Fe, Zn, Mn, Ni, Cr, Cu, Pb, Co and Cd, and in terms of mineral elements such as K, Ca, Mg, Na, Al, and B using ICP-OES. In addition, lipid peroxidation, chlorophyll and carotenoid levels in plant samples were also checked. Sediments in Karasu River contained high concentrations of Ni and Cr metals. Moreover, P. australis showed hyper-accumulation for Cd and Zn in root and leaf samples, respectively. Although there was no significant difference in chlorophyll amounts among locations, lipid peroxidation was found to be high in samples taken from İliç.

    • SDG 6 · Temiz Su ve Sanitasyon
    • SDG 15 · Karasal Yaşam
    RDL Kalıcı Kaydıİncele
  • ArticleYalnız künye2015
    Integrating multiple texture methods and NDVI to the Random Forest classification algorithm to detect tea and hazelnut plantation areas in northeast Turkey

    Akar, Ö.; Güngör, O.

    Hazelnuts and tea are two major agricultural crops grown in the eastern Black Sea region in Turkey. Since this part of Turkey is not industrialized, most of the local people work in agriculture, making hazelnuts and tea a part of their lives. For the government side, it is crucial to keep records of the amount of harvested croplands to implement agricultural policies. In fact, the harvested area and crop type of each cadastral parcel are collected either during cadastral surveys or with the declaration of individual farmers, yet this information is mostly not up-to-date and does not reflect the current land-use status. This study aims to determine the extent and distribution of hazelnuts and tea grown areas using the Random Forest (RF) classification algorithm. Tea and hazelnuts give similar spectral reflectance values to surrounding vegetation, which makes it difficult to distinguish them using only their spectral properties. To tackle this problem, the normalized difference vegetation index (NDVI) and texture extraction methods such as the Grey Level Co-occurrence Matrix (GLCM) and Gabor filter were integrated with the RF algorithm, and their contributions to the success of the RF classification method were examined. WorldView-2 satellite images, which have eight multispectral bands (MS: 2 m) and one higher spatial resolution panchromatic band (PAN: 0.5 m), were used. Since the study area contains agricultural products grown in different seasons, satellite images belonging to both summer and winter periods were used. Preliminary results acquired using only spectral values indicated that the RF method gives 79.05% and 71.84% overall accuracies for summer and winter periods, respectively. Integrating texture information improves the performance of the RF algorithm such that the overall classification accuracies are increased to 83.54% and 87.89% when texture information extracted with GLCM and the Gabor filter is added. The classification performance of the winter image is also boosted to be 77.41% and 79.73% with the contribution of texture information obtained with GLCM and the Gabor filter, respectively. Finally, produced thematic maps were compared with the latest cadastral maps to validate classification results with ground truth data. The obtained results reveal the success of integrating texture features in classification since the created thematic maps are consistent with actual land use. The results also show that the crops grown on some cadastral parcels are not coherent with the most current cadastral database, which implies that the cadastral maps need to be updated.

    • SDG 2 · Açlığa Son
    • SDG 15 · Karasal Yaşam
    RDL Kalıcı Kaydıİncele
  • ArticleAçık Erişim2014
    Assessment of genetic diversity and phylogenetic relationships of endangered endemic plant Barbarea integrifolia DC. (Brassicaceae) in Turkey

    Filiz, Ertuğrul; Osma, Etem; Kandemir, Ali; Tombuloğlu, Hüseyin; Tombuloğlu, Güzin; Birbilener, Seda; Aydin, Mehtap

    Barbarea integrifolia DC. (Brassicaceae) is an endangered and endemic species located in Erzincan and Gümüşhane provinces of Turkey. In total, 27 individuals from 2 natural populations were assessed using the random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) method coupled with sequence analysis of the internal transcribed spacer (ITS1) rDNA region. Genetic accuracy of RAPD-PCR was tested with 25 RAPD primers, and it resulted in 115 clear and reproducible DNA fragments from 13 RAPD primers. Among these, 76 (66.1%) fragments were found to be polymorphic. According to the ITS1 analysis of B. integrifolia populations, the plants exhibited a relatively poor genetic diversity. In total, 60 ITS1 nucleotide sequences from the GenBank database with 2 newly identified ITS1 sequences from the Turkish populations were used to construct a maximum-likelihood tree. The lengths of ITS1 sequences of B. integrifolia were found to be 268 bp for population I and 271 bp for population II. ITS1 sequences of B. integrifolia species showed 97% sequence identity. Phylogenetic analysis revealed that Barbarea species were monophyletic and showed high bootstrap values. B. integrifolia had closer relationships with Cardamineae taxa, including Cardamine, Rorippa, and Armoracia. On the contrary, allied genus Nasturtium species were separated from Cardamineae taxa. The genetic information obtained from this study could be used for the development of conservation strategies not only for B. integrifolia but also for rare and endangered plant species.

    • SDG 15 · Karasal Yaşam
    RDL Kalıcı Kaydıİncele
  • ArticleYalnız künye2013
    Cement dust pollution induces toxicity or deficiency of some essential elements in wild plants growing around a cement factory

    Mutlu, Salih; Atici, Ökkeş; Gülen, Yasir

    In the present study, it was aimed to determine the effects of cement dust pollution on contents of some significant essential elements (P, S, K, Ca, Fe and Cl) in wild plants (Medigago varia, Anchusa leptophylla, Euphorbia orientalis, Lactuca serriola, Artemisia spicigera, Crambe orientalis, Convolvulus sepium and Senecio vernalis) using wavelength-dispersive spectrometer X-ray fluorescence technique. Plant samples were collected from different locations around a cement factory which is located at Askale about 50 km from Erzurum (Turkey). The element contents in the plant specimens that existed in both 0–100 m (dense dusted) and 2000 m (undusted) areas were compared. P, S, K and Cl contents were found to be high in the plants growing in areas 0–100 m from the cement factory, compared to same plants at 2000 m far from the factory. However, Ca and Fe contents were determined to be low in plants growing in 0–100 m area from the factory. Results of the study can contribute to understand how mineral deficiency and toxicity lead to detrimental effects on plant growth and development in the fields contaminated by cement dust.

    • SDG 15 · Karasal Yaşam
    RDL Kalıcı Kaydıİncele
  • ArticleAçık Erişim2013
    Soil-plant relations in the annual Gypsophila (Caryopyhllaceae) taxa of Turkey

    Korkmaz, Mustafa; Özçelik, Hasan

    The soil properties of the annual Gypsophila L. taxa of Turkey and their effects on plant morphology were investigated. The taxa studied were Gypsophila heteropoda Freyn & Sint., G. parva Barkoudah, G. elegans M.Bieb., G. bitlisensis Barkoudah, G. viscosa Murray, G. antari Post & Beauverd, G. muralis L., G. tubulosa (Jaub. & Spach) Boiss., G. confertifolia Hub.-Mor., and G. pilosa Hudson. The soils of the plant taxa were medium textured, sand or sandy-loam, saltless or a little salty, neutral, limy, rich in potassium and with very little phosphorous, and with medium levels of nitrogen and organic matter. G. antari, which had the greatest lime content, had the lowest number of leaves. G. muralis and G. tubulosa had the lowest saturation values and lime content but the greatest number of leaves. If the lime content increased, the development of roots decreased, and when the rate of sand increased, the development of roots increased. For G. muralis, phosphorus concentration, seed size, and salt content were negatively related to number of leaves. For G. tubulosa and G. antari, potassium and organic matter concentrations were negatively related to number of leaves. In G. muralis, salt content was positively related to calyx diameter and seed size. In G. confertifolia, organic matter content was positively correlated with height of the calyx, petal, and bract. It was observed that G. pilosa, G. bitlisensis, and G. viscosa species are also distributed in limeless steppe areas. The most important distribution areas of the species in Turkey are Sivas, Erzincan, Çanki{dotless}ri{dotless}, Eskişehir, and Ankara.

    • SDG 2 · Açlığa Son
    • SDG 15 · Karasal Yaşam
    RDL Kalıcı Kaydıİncele
  • ArticleAçık Erişim2008
    General View to Turkey Forage Crops Cultivation

    Yolcu, Halil; Tan, Mustafa

    Forage crops cultivation in meeting roughage requirement of our animal existence has an important role. Our pastures and rangelands have been destroyed because of excessive and early grazing and not making attention works. Notwithstanding in amount of crossbred and import breed animals compared native breed have been nowadays important increases. Consequently, in order to meet hay requirement of our animal existence apt gradually intensive agriculture, it is necessary increase of forage crops field and yields. With decision 2000/467 numbered council of ministers of Ministry of Agriculture and Rural affairs, in supported forage crops cultivation has occurred important increase. But in these increases is not sufficient to meet requirement hay of available animal existence. Consequently, making supports to increase field and yield of forage crops cultivation have been continued increasing and diversifying. Absolutely seed problem must be solved in forage crops. Farmers must informed about forage crops cultivation and important of forage crops for animal feeding.

    • SDG 2 · Açlığa Son
    • SDG 15 · Karasal Yaşam
    RDL Kalıcı Kaydıİncele