Yazarlar
- Osma, Etem(20)
- Yangılar, Filiz(17)
- Kaya, Ozkan(16)
- Katipoğlu, Okan Mert(15)
- Yangilar, Filiz(15)
- Yildirim, Nalan(15)
- Karakus, Sinem(14)
- Turan, Metin(13)
- Özyürek, Selçuk(13)
- Elveren, Müjgan(12)
- Kandemir, Ali(12)
- Agar, Guleray(9)
- Bulut, Hüseyin(9)
- Ozturk, Halil Ibrahim(9)
- Varol, Tuğçe(8)
- Öztürk, Halil İbrahim(8)
- Haliloğlu, Kamil(7)
- Kılıç, Engin(7)
- Mutlu, Salih(7)
- Yildiz, Faruk(7)
- Akşit, Zeynep(6)
- Cebi, Kadir(6)
- Ertugay, Mustafa Fatih(6)
- Karakaya, Songül(6)
- Kiliçgün, Hasan(6)
- Kumbasaroglu, Hediye(6)
- Köksal, Ekrem(6)
- Türkoğlu, Aras(6)
- Yuca, Hafize(6)
- Achite, Mohammed(5)
- Akşit, Hüseyin(5)
- Atici, Ökkeş(5)
- Demirci, Betül(5)
- Poczai, Peter(5)
- Türkyılmaz, Doğan(5)
- Çebi, Kadir(5)
- Özkan, Güller(5)
- Öztürk, Halil Ibrahim(5)
- Aydın, Bilge(4)
- Bozkurt, Abdurrahim(4)
Yayınlar
- ArticleYalnız künye2024Essential oils in post-harvest disease management: Metabolic impact on Narince (Vitis vinifera L. cv) grapes against Botrytis cinerea
Kaya, Ozkan; Bozkurt, Abdurrahim; Karakus, Sinem; Daler, Selda; Yilmaz, Turhan; Turan, Metin
There is an urgent need for alternative, environmentally friendly post-harvest disease management solutions because of the growing concern over the use of chemical fungicides in agriculture and their detrimental impact on human health and the environment. This study examines the effects of thymol, eugenol, and 1,8-cineole essential oils on the sugar, organic acid, and amino acid profiles of 'Narince' grape berries as well as their effectiveness against gray mold (Botrytis cinerea). Our study has noted that the application of EOs, particularly eugenol, significantly alters the metabolic profile of 'Narince' grapes, enhancing sugar content and modifying the levels of organic acids and amino acids, which suggests a potential for improving grape quality and resistance to Botrytis cinerea. The combined use of eugenol with other EOs and fungicides, as indicated by the treatments OF and OSF, not only displayed a synergistic effect in elevating the concentration of key metabolites but also appeared to be more effective in combating fungal infection compared to individual EO applications. The PCA analysis and subsequent heatmap visualization revealed distinct metabolic clustering among the treatments, with combined EO treatments, especially those including eugenol, markedly influencing the amino acid profile of the berries. Our investigation's results suggest that EOs may offer a viable, eco-friendly alternative to traditional fungicides. Our study also adds to the body of knowledge supporting the advancement of integrated pest management strategies that are efficacious yet environmentally considerate, aligning with the global shift towards more sustainable practices in agriculture and food security.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Zinc Oxide Nanoparticles: An Influential Element in Alleviating Salt Stress in Quinoa (Chenopodium quinoa L. Cv Atlas)
Türkoğlu, Aras; Haliloğlu, Kamil; Ekinci, Melek; Turan, Metin; Yildirim, Ertan; Öztürk, Halil Ibrahim; Stansluos, Atom Atanasio Ladu; Nadaroğlu, Hayrunnisa; Piekutowska, Magdalena; Niedbała, Gniewko
Climate change has intensified abiotic stresses, notably salinity, detrimentally affecting crop yield. To counter these effects, nanomaterials have emerged as a promising tool to mitigate the adverse impacts on plant growth and development. Specifically, zinc oxide nanoparticles (ZnO-NPs) have demonstrated efficacy in facilitating a gradual release of zinc, thus enhancing its bioavailability to plants. With the goal of ensuring sustainable plant production, our aim was to examine how green-synthesized ZnO-NPs influence the seedling growth of quinoa (Chenopodium quinoa L. Cv Atlas) under conditions of salinity stress. To induce salt stress, solutions with three different NaCl concentrations (0, 100, and 200 mM) were prepared. Additionally, Zn and ZnO-NPs were administered at four different concentrations (0, 50, 100, and 200 ppm). In this study, plant height (cm), plant weight (g), plant diameter (mm), chlorophyll content (SPAD), K/Na value, Ca/Na value, antioxidant enzyme activities (SOD: EU g−1 leaf; CAT: EU g−1 leaf; POD: EU g−1 leaf), H2O2 (mmol kg−1), MDA (nmol g−1 DW), proline (µg g−1 FW), and sucrose (g L−1), content parameters were measured. XRD analysis confirmed the crystalline structure of ZnO nanoparticles with identified planes. Salinity stress significantly reduced plant metrics and altered ion ratios, while increasing oxidative stress indicators and osmolytes. Conversely, Zn and ZnO-NPs mitigated these effects, reducing oxidative damage and enhancing enzyme activities. This supports Zn’s role in limiting salinity uptake and improving physiological responses in quinoa seedlings, suggesting a promising strategy for enhancing crop resilience. Overall, this study underscores nanomaterials’ potential in sustainable agriculture and stress management.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Physicochemical, bioactive, microbial, and sensory characteristics of homemade vinegar produced with a local apple variety using a traditional method
Kiliçgün, Hasan; Yangilar, Filiz
Apple is one of the most widely grown fruits in the world and can be found fresh in the market throughout the whole year due to its excellent storage ability. In addition to fresh consumption, apples are processed into a variety of products including fruit juice, vinegar, jams, and pies. In this study, the bioactive contents and microbial, physicochemical, and sensory properties of seven vinegars traditionally produced using a local apple variety, Kara Sakı, with different yeast materials (Saccharomyces cerevisiae, chickpea, buckwheat, barley, whey, natural home apple vinegar, and commercial apple cider vinegar) were assessed. The results indicated significant differences among vinegar samples for dry matter, ash, pH, total acidity, degrees Brix, alcohol, viscosity, total sugar, and color values. The total antioxidant capacity (DPPH assay as %), total polyphenolic contents, and total flavonoid contents of the seven apple vinegars were in range of 29.17%–82.64%, 44.45–470.30 mg gallic acid equivalent/100 mL, and 34.82–81.02 mg quercetin equivalent/100 mL, respectively. Total mesophilic aerobic bacteria, mold and yeast, lactic acid bacteria, and acetic acid bacteria counts ranged from 2.62 to 5.53 log CFU/mL, 1.0 to 3.4 log CFU/mL, 1.01 to 3.85 log CFU/mL, and 1.35 to 4.48 log CFU/mL, respectively. The sensory evaluation results showed that the panelists preferred the commercial, natural, and chickpea vinegars the most. The findings suggest that the use of traditional processes for the production of fruit vinegars is promising in terms of producing differentiated vinegars and obtaining high levels of human health-promoting contents.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Exploring the potential of bilberry extracts as natural antifungal and bioherbicidal agents in agriculture: composition and antioxidant activity; [Istraživanje potencijala ekstrakta borovnice kao prirodnih antifungalnih i bioherbicidnih agenasa u poljoprivredi: sastav i antioksidativna aktivnost]
Bayar, Yusuf; Yılar, Melih; Akşit, Hüseyin; Genç, Nusret
This study aims to investigate the composition of the leaf essential oil and the total phenolic and flavonoid contents as well as the antioxidant activity of the n-hexane, ethyl acetate, and methanol extracts of Vaccinium myrtillus leaves collected from two different locations (Muğla and Mersin, Turkey). In addition, the antifungal activity against Sclerotinia sclerotiorum (SS), Fusarium oxysporum f sp. melonis (FOM), Fusarium oxysporum f sp. cucumerinum (FOC), and Rhizoctonia solani (RS) and the bioherbicidal activity against Taraxacum officinale and Rumex crispus were evaluated for the first time. The methanolic extract inhibited mycelium growth of SS, FOM, FOC, and RS in a dose-dependent manner. No significant difference was observed in antifungal activity between the two different collection sites. In the bioherbicidal activity tests, the methanol extract of the fruits completely suppressed the root-shoot development of Taraxacum officinale, while also significantly inhibiting the root-shoot development during the seed germination of Rumex crispus compared to the control group at 3 mg/mL concentration for both locations. Based on the results of the GC/MS analysis, the major constituents identified in the leaf essential oils collected from the Muğla and Mersin locations were α-pinene (29.16%/15.75%), eucalyptol (22.19%/26.46%), linalool (12.66%/25.27%), and linalyl acetate (7.43%/2.71%). The total phenolic and flavonoid contents of the plant extracts for the Muğla and Mersin samples were determined as (42.80‒157.58 GAE/g extracts), (29.38‒151.44 GAE/g extracts) and (10.52‒37.88 QE/g extracts), (14.81‒44.18 QE/g extracts) respectively. In addition, the plant exhibited significant antioxidant activity. These findings indicate quantitative differences in the chemical composition of the essential oils between the two geographical locations. These findings are significant as they provide insights for the development of new natural antifungal agents with potential applications in agriculture.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Fatty acids, Vitamins and Antioxidant Properties of Cladophora fracta var. intricata; [Cladophora fracta var. intricata’nın Yağ Asitleri, Vitamin ve Antioksidan Özellikleri Tuğba]
Yücer, Demiriz; Pabuçcu, Köksal
In this study investigations of the fatty acids, vitamins and antioxidant properties of cultured (CFI) Cladophora fracta var. intricata. CFI was isolated from benthic habitats of the Tokat Yeşilırmak River (Tokat) and axenic cultured. According to the antioxidant analysis results, a dose-dependent activity was observed in CFI in terms of DPPH free radical scavenging activity, FRAP and TEAC reducing power, but it was not found to be significant as it remained above IC50 values. In CFI, C14:0 Myristic acid, C16:0 Palmitic acid and C16:1 Palmitoleic acid, C18: 1 N9C Oleic fatty acids and vitamins A, C, E were found to be high. According to antioxidant analysis results, CFI was found to be notable for the use of its fatty acid and vitamin values in food and pharmaceutical. Since it has low contamination properties, the algal taxon examined can be easily used in culture media.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Enhancing Post-Harvest Resilience: Investigating the Synergistic Effects of Essential Oil Combinations on Biochemical Profiles in Botrytis cinerea-Infected Apples
Karakus, Sinem
This study explored the impact of essential oil combinations on Botrytis cinerea-infected apples, focusing on how these treatments affect the levels of amino acids, anthocyanins, organic acids, phenolic compounds, and hormones. We discovered significant differences in amino acid concentrations, particularly asparagine, serine, histidine, glycine, and arginine, between control and fungus-treated apples. Preventive measures notably increased anthocyanins like delphinidin-3-glycoside and peonidin-3-glycoside, while combinations such as thymol + fungus balanced anthocyanin profiles effectively. Organic acid and phenolic compound analyses showed that curative strategies generally increased concentrations, with the thymol + cineole + fungus treatment being especially effective. Hormonal analysis highlighted the benefits of preventive measures in raising indole-3-acetic acid and gibberellic acid levels, whereas curative treatments increased abscisic acid and salicylic acid concentrations. The combination of cineole and thymol with fungicide emerged as a potent strategy for enhancing phenolic content. These findings underscore the potential of specific essential oil combinations in improving the biochemical composition of B. cinerea-infected apples, offering new avenues for enhancing fruit quality and sustainability in the agriculture sector.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Effects of Nettle (Urtica dioica) Extract on Versus Pathogenic Microorganisms in Yogurt Production; [Yoğurt Üretiminde Isırgan Otu (Urtica dioica) Ekstraktının Patojenik Mikroorganizmalara Karşı Etkileri]
Gülhan, Barış; Yangilar, Filiz
Contamination with pathogenic bacteria is the most common cause of foodborne illness and represents a public health problem worldwide. These pathogens can be controlled by adding extracts to fermented milk. In the present study, the effects of nettle extract on selected pathogenic bacteria in yogurt production were investigated. For this purpose, the antibacterial effectiveness of the extract additives in the presence of bacteria was examined and compared. Yoghurt samples, 24 different samples contaminated with 7 standard pathogen strains, were analyzed for chemical properties (pH and acidity) and antimicrobial activity after 1, 7, 14 and 21 days of storage at 4 °C. In particular, it was found that the decrease in pH and increase in acidity in the nettle extract samples after 21 days were significantly greater than in natural yogurt and yogurt samples containing nettle extract (0.5% and 1%, respectively) (p<0.05). Pathogens were reduced more significantly in samples treated with nettle extracts, which have antibacterial activity against pathogens. In particular, compared to natural yogurt, these samples showed significant antibacterial effects on the standard strains Streptococcus pneumoniae ATCC 45615, Klebsiella pneumoniae ATCC 70063 and Pseudomonas aeruginosa ATCC 27853 (p<0.01). Furthermore, in the samples containing nettle extract, the 1% concentration had synergistic effects on Streptococcus pneumoniae ATCC 45615, while the 0.5% concentration had synergistic effects on Klebsiella pneumoniae ATCC 70063 and Pseudomonas aeruginosa ATCC 27853. Overall, these results suggest that the nettle extract could be used as a natural preservative to improve the safety of yogurt and other dairy products.
RDL Kalıcı Kaydıİncele - Review ArticleYalnız künye2024An overview of the roles of critical insiders and outsiders for reciprocal plant–microbe interaction: Heterotrimeric G-proteins, small RNAs, pollinators, microalgae
Avşar, Bihter; Erendemir, Eda; Taşkin, Zeynep
Plants are exposed to various abiotic stresses, which lead to crop losses and become a significant threat to agriculture worldwide. To survive, they have developed a range of mechanisms throughout their life cycle. While they develop immunity against stressors through their metabolic and hormonal pathways, as another strategy, they can also modify their environment by interacting with symbiotic microorganisms involved in the critical pathways for plant health. Several beneficial microorganisms can be used as plant stimulants to augment plant health and growth. Therefore, elucidating the cause-effect relationship and possible roles of critical players during plant–microbe interaction may help us better understand the usage of microorganisms for plant benefits. The presented review discusses the molecular mechanisms regulating the responses of plants and their environment during plant–microbe interactions. We focus on the potential roles of plant heterotrimeric G-proteins and small RNAs as inside players and the possible roles, connections, and effects of microalgae and pollinators as outside players/elements during plant–microbe interactions under abiotic stress. Utilizing microbial inoculants with a better combination of endophytes based on various plant species/populations under different environmental conditions is critical for successful field applications. The current knowledge in this review may provide a detailed assessment to gain insight into unraveling different parameters for efficient use of the endophytes on plants/crops to address food security challenges and mitigate the impact of climate change on agriculture.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2024Virtual insights into the quercetin- Melampsora lini-derived effector AvrM14 interaction: An In silico exploration of plant defense mechanisms
Shadidizaji, Azizeh; Tolga Cinisli, Kağan; Warda, Mohamad; Cicek, Betul; Hacimuftoglu, Ahmet
The co-evolutionary struggle between plants and pathogens shapes vital defense mechanisms crucial for survival. AvrM14 selectively removes the 5′ cap from mRNA, disrupting key aspects of plant mRNA regulation, enhancing the flax rust fungus's infection capabilities. In contrast, quercetin, a plant flavonoid, serves as an antioxidant, stress reliever, and immune protector. This in silico study explores quercetin's inhibition of AvrM14, revealing high-affinity binding to the Nidux domain through molecular docking. Confirming stability in dynamic simulations, this pioneering research establishes quercetin as a functional AvrM14 inhibitor, suggesting its potential as a therapeutic agent against Melampsora lini's harmful impact on plant health.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Influence of nitrate levels on plant growth and carbon and nitrogen metabolism in cucumber (Cucumis sativus L.) under low light stress
Shah, Syed Aizaz Ali; Shi, Yuzi; Nie, Jing; Yao, Xuehui; Guo, Yicong; Ahmad, Muhammad; Shah, Syed Atizaz Ali; Hayat, Faisal; Niamat, Yasmin; Öztürk, Halil İbrahim
Photosynthesis is critically dependent on both adequate light and nitrogen availability. In controlled agricultural environments with limited natural light, cucumber faces substantial challenges, including reduced photosynthetic efficiency and biomass accumulation. Nitrogen (N) is a vital component for the synthesis of various macromolecules, metabolites, and signaling molecules in plants. This study assessed the impact of different nitrate levels (LL-N, LL-1/2N, and LL-2N) on the physiological, biochemical, and metabolic responses of cucumber under low light conditions (80–100 µmol m–2 s–1 photosynthetic photon flux density) in comparison to standard light (400–500 µmol m–2 s–1 photosynthetic photon flux density) and unchanged nitrate. We observed that increasing nitrate levels to twice the standard concentration (LL-2N) significantly enhanced shoot morphology, including weight and size, and improved photosynthetic performance, stomatal conductance, and transpiration rates. In contrast, lower nitrate levels (LL-1/2N) exacerbated stress, leading to diminished photosynthesis and biomass. Biochemical analyses indicated that elevated nitrate concentrations activated key enzymes involved in the metabolism of carbon and nitrogen, including glutamine synthetase, sucrose phosphate synthase, nitrate reductase, and glutamate synthase. This led to improvement in the amounts of sucrose, fructose, soluble sugars, free amino acids, and soluble proteins. Additionally, the activities of antioxidant enzymes including superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase were considerably greater under conditions of increased nitrate, which reduced the levels of malondialdehyde and H2 O2 as oxidative stress markers. Furthermore, enhanced nitrate levels upregulated critical photosynthetic genes (rbcL, rbcS, FBPase, and SBPase mRNA), supporting the RuBisCO contents and overall photosynthetic capacity. These findings underscore the effectiveness of strategic nitrate enrichment in mitigating low light stress, providing essential insights for optimizing cucumber cultivation in controlled environments and improving productivity.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2024Improving Biogenic Amines and Combating Botrytis cinerea Decay in ‘Golden Delicious’ Apples With Post-Harvest Essential Oil Treatments
Karakuş, Sinem; Kaya, Ozkan; Şahin, Müge
Malus Domestica Borkhausen, commonly known as ‘Golden Delicious’ apples, are highly favored for their nutritional attributes, including crisp texture, robust flavor, sweetness, firmness, color, and a rich array of phytochemical compounds, predominantly polyphenols. Despite their popularity, apples have a limited shelf life, particularly when stored at room temperature. EOs have emerged as a secure postharvest technology with the potential to mitigate post-harvest losses and preserve both labile constituents and overall quality. This study sought to assess the impact of EOs treatments on BAs within ‘Golden Delicious’ apple cultivar and their efficacy in combatting Botrytis cinerea during both protective and curative phases. Interestingly, a strong positive interplay was observed among Cad, His, and Try, while these BAs exhibited a negative association with Dop, Tma, and Nor. EOs treatments exhibited a general tendency to augment the levels of BAs during both preventive and curative periods. Put, Cad, His, and Try were identified as the predominant BAs in ‘Golden Delicious’ apple cultivar during the preventive and curative phases. The interrelation between EOs treatments and application timelines was found to be significantly associated with the levels of BAs, particularly involving combinations such as Eug + Fun, Cin + Fun, Thy + Eug + Fun, Thy + Cin + Fun, Thy + Cin + Eug + Fun, and curative phases. The outcomes of the investigation revealed that the combination of Fun + Thy + Cin + Eug treatments during the protective and curative phases was notably effective in reducing the incidence of fruit decay attributed to B. cinerea. Conversely, the study established correlations with terms such as Fungus, Cin + Eug + Fun, Thy + Fun, control, and protective. In conclusion, the research indicates the dual functionality of EOs application, both as a preventive measure against pathogenic agents such as B. cinerea and as a regulator of potentially harmful BAs within the apples. These findings hold promise for improving the quality and safety of ‘Golden Delicious’ apples in the marketplace, addressing both shelf life and consumer health considerations.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2024Preserving the richness of nature: cultural and ecological importance of edible wild plants in Sivas
Kadioglu, Zakine; Yildiz, Faruk; Kandemir, Ali; Cukadar, Kemal; Kalkan, Nalan Nazan; Vurgun, Huseyin; Donderalp, Veysel; Korkut, Recep; Kaya, Ozkan
This comprehensive article offers a thorough exploration of the abundant plant species that hold a deep connection with the history and daily life of Sivas, within the context of its traditional culture and ethnobotany. The Asteraceae family, which encompassed 22 species, emerged as the most diverse family. Other notable families included Brassicaceae (8 species), Fabaceae (9 species), Polygonaceae (10 species), Apiaceae (11 species), and Lamiaceae (21 species), and the study systematically classified 128 plant species and subplants into 29 different families. The study categorized these plants based on their edible parts to understand their role in Sivas’ culinary traditions, revealing a wide range of edible components, including leaves, seeds, flowers, roots, tubers, gum, fruits, branches, and more. Cerinthe minor, Berberis vulgaris, Stachys lavandulifolia and others provided nourishing leaves and young shoots. The study categorized plants based on their preparation methods, highlighting their integral role in Sivas’ traditional cuisine. Seeds namely Echinops orientalis and Cephalaria procera introduced culinary diversity, while certain plants, such as Geranium tuberosum and Cirsium rhizocephalum, contributed edible roots, expanding the repertoire of local dishes. Whether utilized in cooked dishes, salads, or as spices, these plants impart unique flavors and aromas to the local culinary creations. Whether used in cooked dishes, salads, or as spices, these plants add unique flavors and aromas to local food. Spices such as Mentha longifolia and Thymus leucotrichus enriched the culinary landscape, while Thymus sipyleus and Ziziphora clinopodioides brought their distinct tastes to dishes. The repeated use of common names across different species, such as “Yemlik” “Yağlıca” “Nane” and “Kekik” suggests a shared cultural heritage and linguistic connection among these plants, further emphasizing their significance in the local context. Five species have been newly documented as additions to the flora of Sivas. In summary, this study underscores the importance of preserving and appreciating the botanical knowledge and practices of Sivas traditional culture and ethnobotany. The numerical values within the findings quantitatively showcase the ecological and cultural richness of these plant species, thus making this subject a vital area of study for researchers and a valuable resource for conservationists aiming to safeguard this unique cultural and botanical heritage.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2024Antioxidant potential and stability of bovine liver peptides in fermented sausage (sucuk): A pathway to value-added functional meat products
Arslan, Betul; Xiong, Youling L.; Soyer, Ayla
The objective of the present study was to evaluate the stability of low-molecular-weight peptides after their incorporation into fermented sausages. Peptide fractions were produced from the bovine liver peptides (BLP) by enzymatic hydrolysis. Optimal conditions were identified as hydrolysis at 45 °C for 24 h, resulting in the generation of <3 kDa and <10 kDa peptide fractions exhibiting the highest antioxidant activity. The antioxidant potential of fermented sausages made from beef meat containing BLPs at 0.5% and 1% concentrations was assessed during manufacturing and storage. The initial antioxidant activity values of the sausages containing BLPs were higher than that of the control. Furthermore, the stability of the peptides in the sausages was demonstrated by their preserved antioxidant activity for 102 days. Peptides extracted from 1% of <10 kDa-peptide containing sausages possessed two-fold higher radical scavenging activity than control. The highest peptide concentration in the sausages containing 1% of <10 kDa-peptides (79.32±1.34–101.56±1.22 μg peptide/mg) was maintained from day 0 to day 102. The SDS-PAGE profile of myofibrillar proteins and peptide extracts revealed the degradation of proteins were delayed in <10 kDa of peptide added sausages, suggesting that the protein oxidation was slowed down. These results contribute to the advancement of peptide commercialization as functional ingredients in fermented food products. Furthermore, our research sheds light on the enduring impact of fermentation on the stability of the added peptides and the synergistic interactions between naturally produced peptides during fermentation and those introduced externally.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Physico-Chemical Responses of Alstroemeria spp. cv. Rebecca to the presence of Salicylic Acid and Sucrose in vase solution during postharvest life
Seyed Hajizadeh, Hanifeh; Bayrami Aghdam, Somayeh; Fakhrghazi, Hadi; Karakus, Sinem; Kaya, Ozkan
Background: The primary challenge in the cut flower industry, specifically in the postharvest phase, is the short vase life of flowers. This issue, along with early leaf yellowing and perianth abscission, significantly diminishes the economic value of flowers due to their accelerated senescence. To tackle this, we conducted a factorial experiment on Alstroemeria cv. Rebecca, utilizing a completely randomized design with three replications. In this experiment the effects of varying concentrations of Salicylic acid (SA) (0, 1.5, and 3 mM) and sucrose (SU) (0% and 3%) were investigated on the postharvest quality of leaves and florets, with systematic evaluations every three days throughout their vase life. Results: This experiment revealed that the specific treatment combination of 1.5 mM SA + 3% SU (T5) markedly improved various parameters, such as vase life, total chlorophyll content, membrane stability index, relative fresh weight, and water uptake of cut flowers. In our analysis, we observed that this preservative solution not only extended the vase life and enhanced water uptake but also effectively preserved total chlorophyll, mitigated the loss of fresh weight, and reduced membrane deterioration in petals. Additionally, our results showed an increase in the activities of catalase (CAT) and peroxidase (POD) enzymes, as well as total protein content, alongside a decrease in malondialdehyde (MDA) and hydrogen peroxide (H2O2) levels. Moreover, this study noted a decrease in microbial populations in solutions containing different concentrations of salicylic acid. Conclusions: Our research demonstrated that alstroemeria flowers maintained in a solution with 1.5 mM SA + 3% SU exhibited a significantly prolonged vase life of up to 21 days, in contrast to the 15 days observed in control flowers kept in water. These results are highly beneficial for manufacturers in the cut flower industry, as they provide a viable method to substantially extend the vase life of cut flowers. Such an enhancement in flower longevity can lead to increased market value and customer satisfaction. Furthermore, the reduction in flower senescence and decay rates can contribute to decreased waste and greater efficiency in cut flower distribution and sales, offering a substantial advantage to manufacturers in this competitive market. The extended vase life and reduced senescence observed in alstroemeria flowers treated with 1.5 mM SA and 3% SU are attributed to SA's role in enhancing endogenous defense responses and sucrose's function as an energy source, collectively improving water uptake, and delaying the natural decay process.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Comparative content analysis and biological activity studies of fatty and essential oils from some garlic products sold in Turkish community pharmacies, natural, and fermented garlic
Deniz, Elif Ulutaş; Yuca, Hafize; Aydın, Bilge; Öztürk, Gözde; Çoban, Furkan; Göger, Gamze; Demirci, Betül; Karakaya, Songül
Garlic (Alliaceae), an annual herb, is renowned not only for its distinctive flavor but also for its extensive therapeutic applications in managing various ailments and health conditions. In this study, garlic products identified as the best-selling items in Turkish pharmacies for various purposes were compared with garlic grown under standard conditions in terms of chemical composition and antidiabetic, anticholinesterase, antimicrobial, and antioxidant properties. Three of garlic samples were prepared by researchers. According to survey results, the most commonly sold garlic-related products in pharmacies are black garlic extract tablets (4), capsules (5), garlic oil (6), garlic oil pearls (7), and fermented garlic (8). Diallyl disulfide (DADS) was identified as a predominant compound in sampled oils, ranging from 4.9% to 48.6%. Another noteworthy finding is the identification of allyl methyl disulfide and allyl methyl trisulfide as major components in sampled oils, with concentrations spanning from 2.9% to 9.8% and 0.4% to 17.5%, respectively. In both 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS·+) and 2,2-diphenyl-1-picrylhydrazyl (DPPH•) tests, Sample 1 (fermented) exhibited the highest antioxidant activity. Sample 3 (cultivated) was richer in total phenol–total tannin content. Sample 6 exhibited the highest α-glucosidase inhibition among antidiabetic activities, reaching 28.93%. Sample 5 capsules demonstrated the highest α-amylase inhibition at 51.50%. Sample 7 exhibited the most notable inhibition against both acetylcholinesterase (22.92%) and butyrylcholinesterase (13.37%). Samples 3, 6, and 8 were found to be more effective against Candida tropicalis with minimum inhibitory concentration (MIC) = 625 μg/mL. A comprehensive study on garlic products, including popular items from Turkish pharmacies and those grown under standard conditions, revealed diverse chemical compositions and multifaceted health properties.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024The correlation between children’s health literacy level and healthy eating self-efficacy
Kasimoğlu, Necla; Gürarslan Baş, Nazan
Background The school environment provides an important opportunity for children and educators to develop health education and health literacy the ability to make rational health decisions and identify and determine factors that affect health. The aim of this study is to determine the correlation between health literacy and eating self-efficacy levels of primary school students and the affecting factors. Methods The study was conducted with 486 fourth graders studying in a primary school located in eastern Turkey between September 2021 and June 2022. The data were collected through face-to-face interviews using the Personal Information Form, the Health Literacy for School-Age Children Scale (HLSAC), and the Healthy Eating Self-Efficacy Scale for Children (HESES-C). Data were analyzed by number, percentage, mean, independent samples t-test, non-parametric Mann–Whitney U and Kruskal–Wallis tests, and Pearson’s correlation tests. Results The gender of the students and the education level of their mothers had a statistically significant correlation with their mean HESES-C and HLSAC scores scores (p<0.05). There was a significant negative correlation between the students’ HLSAC and their HESES-C mean scores. Conclusions According to the findings of the study, the students had a moderate level of health literacy and a low level of healthy eating self-efficacy. There was a negative relationship between health literacy levels and healthy eating self-efficacy.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Pollination Strategies and Reproductive Biology of Fritillaria imperialis L. (Liliaceae): Insights from Erzincan, Türkiye
Yildiz, Faruk; Aslay, Meral; Kaya, Ozkan
Fritillaria spp., comprising perennial bulbous plants of significant medicinal and ornamental value, face high endangerment in their natural habitats. Despite their importance, the reproductive characteristics and adaptive evolution mechanisms of these species remain incompletely understood. This study focused on the pollination strategies and reproductive biology of Fritillaria imperialis L. The research was conducted at the Erzincan Horticultural Research Institute in Türkiye. Our investigation categorized the flowering process of F. imperialis into nine distinct phases. Through comprehensive assessments of the pollen/ovule ratio, self-incompatibility index (SII), and ex situ pollination experiments, we observed high levels of self-incompatibility and allogamy in F. imperialis. Our findings revealed that pollination of F. imperialis primarily relied on pollen vectors, with Apis mellifera and Bombus terrestris identified as the most effective pollinators. Furthermore, average seed set rate, seed production, and seed viability were quantified at 80.5%, 228 seeds, and 86.3%, respectively. The average numbers of pollen viability and pollen grains were measured at 93% and 702,000, respectively. This comprehensive analysis of the reproductive biology of F. imperialis provides crucial insights for the conservation and genetic management of this highly valuable species. The findings contribute to a deeper understanding of the reproductive strategies employed by Fritillaria spp., which may inform future conservation efforts and breeding programs for these endangered plants.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2024Evaluation of α-glucosidase inhibitor activity and bioactive compounds in purple wheat flour yogurts
Çebi, Kadir; Yangılar, Filiz
Nowadays, the food industry attaches more importance to studies on using purple components in food formulations due to their bioactive properties. This study evaluated the phenolic compound content, antioxidant activity, physicochemical properties, sensory properties, and α-glucosidase activities in yogurt enriched with purple wheat flour. Yogurts were produced using varying concentrations of purple wheat flour labeled as sample A (1.5 %), sample B (3 %), and sample C (4.5 %). It was observed that incorporating purple wheat flour led to a decrease in pH and an increase in L*, a*, and b* values, as well as the acidity and viscosity of the yogurts. Sample C contained the highest phenolic content (37.6 mg GAE/100 g dry matter) on day 14, along with the highest flavonoid content (14.59 mg CE/100 g) on day 21 when than control yogurt. In addition, sample C had the highest α-glucosidase activity (38.35 %) on day 14 and anthocyanin content (13.55 g/100 g) on day 21. As a result, C yogurt can be consumed as a diabetic product with antidiabetic and antihypertensive properties by reaching optimal α-glycosidase inhibition activity. Furthermore, yogurts containing purple wheat flour received higher sensory scores from panelists.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Assessment of the Antioxidant, Antiproliferative and Antityrosinase Potential of Unripe Fruit of Prunus x domestica L.
Güven, Zühal Bayrakçeken; Yuce, Neslihan; Basaran, A. Ahmet
Prunus x domestica L. is a valuable plant that belongs to the Rosaceae family and is distributed worldwide. The aim of this study is to investigate the biological effect of the unripe green fruits of P. domestica, which have only been studied to a limited extent so far. The antioxidant capacity of the fruit extracts and subfractions was investigated by DPPH, TEAC and CUPRAC assays. The antiproliferative effect was investigated against the cell lines L929, CaCo-2 and PC-3. While the highest antiproliferative effect against cancer cell lines was found in the unripe fruit ethyl acetate subfraction, extracts and fractions showed no cytotoxic effect on the healthy cell line L929. The fruit ethyl acetate fraction showed a strong inhibition of the enzyme tyrosinase with an IC50: 51.83 μg/ mL compared to standard compound kojic acid (IC50: 21 μg/mL). It was found that the methanol extract from unripe fruits as well as the ethyl acetate and aqueous subfractions exhibited strong antioxidant activity, showed concentration-dependent cytotoxic activity on PC-3 and CaCo-2 cells, but had no cytotoxic effect on healthy L929 cells. The high antioxidant capacity of the fruits, their selective cytotoxic effect on prostate and colon cancer cells and their strong tyrosinase inhibitory activity suggest that they could be a new, safe and cost-effective source for the pharmaceutical and cosmeceutical industries.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2024Essential oil compounds modulate nutritional quality and stress response in Botrytis cinerea-infected grape (Vitis vinifera L. cv. ‘Karaerik’)
Kaya, Ozkan; Bozkurt, Abdurrahim; Karakus, Sinem; Daler, Selda; Yilmaz, Turhan
Grapes, rich in vitamins and minerals, could offer a healthy alternative. However, their potential contamination by the fungus Botrytis cinerea poses serious health risks, necessitating cautious consumption, particularly among those on restricted diets, to mitigate adverse effects. Therefore, the study investigated the effects of individual and combined application of individual components of essential oils (EOs) such as thymol, eugenol, and 1,8-cineole on various parameters such as antioxidant enzymes, mineral elements, anthocyanins, and phenolic compounds in the treatment of B. cinerea infection in 'Karaerik' grapes. Our study provided important information about physiology and pathogen response, and results indicated that the pathogen-infected groups had higher levels of oxidative stress indicators, such as proline, malondialdehyde (MDA), and hydrogen peroxide (H2O2). On the other hand, EOs elevate the activity of antioxidant enzymes, notably superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), whereas thymol treatments effectively reduced oxidative stress to levels comparable to the control group. Under thymol and eugenol treatments, nitrogen (N), potassium (K), phosphorus (P), calcium (Ca), magnesium (Mg), sulfur (S), zinc (Zn), manganese (Mn), boron were significantly higher than the control groups. Increases were also determined in the concentrations of mineral elements such as (B) and copper (Cu). Anthocyanin profiles were preserved by EO treatments, with combined EO applications yielding higher anthocyanin profiles percentages compared to individual treatments. In our results, there were notable differences in the overall quantities of phenolic compounds, and the combination of EO applications caused higher levels of phenolic compounds, especially in the groups that include the fungal infection. Overall, the study showed how effective EO treatments are at reducing B. cinerea infections and maintaining grape quality, which has significance for long-term disease control plans and maintaining berry quality in viticulture.
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